RESUMEN
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the standard procedures used for the isolation of TM cells from several animal species including humans, and the methods used to validate their identity. Having a set of standard practices for TM cells will increase the scientific rigor when used as a model, and enable other researchers to replicate and build upon previous findings.
Asunto(s)
Técnicas de Cultivo de Célula , Separación Celular/métodos , Guías como Asunto , Malla Trabecular/citología , Factores de Edad , Animales , Biomarcadores/metabolismo , Consenso , Feto , Humanos , Donantes de Tejidos , Conservación de Tejido , Recolección de Tejidos y Órganos , Malla Trabecular/metabolismoRESUMEN
The identification of a new class of potent and selective ROCK-II inhibitors is presented. Compound 5 (SR-3677) had an IC 50 of approximately 3 nM in enzyme and cell based assays and had an off-target hit rate of 1.4% against 353 kinases, and inhibited only 3 out of 70 nonkinase enzymes and receptors. Pharmacology studies showed that 5 was efficacious in both, increasing ex vivo aqueous humor outflow in porcine eyes and inhibiting myosin light chain phosphorylation.
