Virgin coconut oil - its methods of extraction, properties and clinical usage: a review.

Abstract: Virgin coconut oil (VCO) is a processed edible oil, which is removed from the mature coconuts. It is a colourless water insoluble liquid and obtained by the hot and cold extraction processes. The nutritional components of VCO are mainly contributed to by lauric acid, its primary content. VCO has shown its anticancer, antimicrobial, analgesic, antipyretic and antiinflammatory properties. Because of these medicinal properties, VCO has gained the wider attention among the medical field. Most evidently VCO has shown its potential antioxidant property, because of its phenolic compounds and medium chain fatty acids. It is one of the beneficial compounds used to prevent and treat the oxidative stress induced neurological disorders like stress, depression and Alzheimer's disease. Dietary supplementation of VCO is easy and economical and safer in daily life among all age groups. It is also beneficial for the cardiovascular, respiratory, dermatological, reproductive and bone health. It can also be applied to the skin as a moisturizer in the paediatric age group. Hence, exploration of antioxidant property as well as other beneficial effects of VCO in various health conditions will be valuable.

Cone Beam Computed Tomography in observing the presence and location of canalis sinuosus.

OBJECTIVE: Cone Beam Computed Tomography (CBCT) was used to observe and describe the distribution of canalis sinuosus (CS) in the Chinese population and the location of CS in the maxillary alveolar bone, so as to help oral surgeons evaluate the intraoperative risk and prognosis before maxillary surgery and reduce the complications caused by the injury of this structure in anterior surgery. PATIENTS AND METHODS: CBCT images of 600 patients admitted from 2021 to 2022 were collected to observe the anatomical structure of CS in the maxillary region. The following parameters were recorded: age, sex, number of CS, left and right distribution of CS, CS diameter, and location. Statistical analysis was performed on all of the collected data. RESULTS: The discovery rate of CS in this study was 59.75%, and it is commonly found in the lateral incisor area (64.82%). No significant difference can be found in the presence and number of CS in different gender and age groups (p>0.05). CONCLUSIONS: The use of high-resolution CBCT before implantation is of irreplaceable significance in the diagnosis and analysis of CS, which is conducive to reducing implantation complications and failure rate. The incidence of CS was independent of age or sex, while the location of CS was statistically significant.

Battery waste-derived magnetic Fe-Mn-Zn/C composites as an electro-Fenton-like catalyst for the degradation of sodium dodecyl sulfate surfactant.

A batch-scale electro-Fenton (EF) process was performed using graphite anode and waste battery-based Fe -Mn -Zn/C electrocatalyst coated on low-cost graphite felt cathode. The effectiveness of the EF's performance was evident with around 83.9 + 4.1% removal of 20 mg/L of sodium-dodecyl sulfate surfactant (SDS) at an optimum current density (CD) of 5.0 mA/cm2, Na2SO4 of 0.05 M, initial pH of 7.2, and electrolysis time of 180 min. Moreover, nearly 1.78-fold more removal of SDS was achieved in EF than in the electro-oxidation process operated without any catalyst. The operating cost of 0.35 $ of per m3 per order was needed to treat SDS wastewater. The remediation of SDS follows pseudo-first-order kinetics with a rate constant of 0.0095 min-1. Additionally, 90.3 + 2.1% of SDS and 57 + 2.6% of total organic carbon (TOC) removal was attained during 240 min of treatment time in secondary treated real wastewater; hence, additional 60 min of treatment time is required for effectively treating real wastewater than synthetic wastewater. Thus, EF is effective with battery waste-derived magnetic catalyst for treating wastewater containing SDS, which can lead to achieving sustainable environmental goals.

Effects of bed depths and the ratio of aerobic to anaerobic zone on the performance of horizontal subsurface flow macrophyte-assisted high-rate vermifilters treating synthetic brewery wastewater.

Effects of total vermibed depth, as well as the ratio of aerobic (the unsubmerged) to anaerobic (the submerged) zone on the performance of the horizontal subsurface flow macrophyte-assisted vermifilters (HSSF-MAVFs) treating synthetic brewery wastewater at a higher hydraulic loading rate (HLR), were investigated for the first time. Results showed that the HSSF-MAVF with a 50 cm total and 18 cm submerged vermibed depth yielded the optimum removal of the pollutants, ensuring a (91.2 ± 1.7)%, (81.8 ± 1.9)%, (67.4 ± 3.9)%, and (63.1 ± 2.3)% removal of chemical oxygen demand (COD), ammonium N (NH4 + -N), total N (TN), and organic N, respectively, whereas there was an increase of (142 ± 6.3)% in the effluent nitrate-N (NO3 - -N) than that in the influent. At the optimum condition, the effluent concentrations of all the pollutants including COD, NH4 + -N, NO3 - -N, TN, and organic N were well below the surface water discharge standards specified by the Central Pollution Control Board (CPCB), and thus, the effluent of the HSSF-MAVF could be safely discharged into the surface water bodies. PRACTITIONER POINTS: Total vermibed depth of HSSF-MAVFs was optimized for organic and nitrogen removal. HSSF-MAVFs were subjected to the higher HLR of synthetic brewery wastewater. Removal of COD and NH4 + -N was decreased with the increase in submerged bed depth. Removal of organic N and TN was increased with the increase in submerged bed depth. Total/unsubmerged bed depth had a positive impact on the organic and N removal.

3D electro-Fenton augmented with iron-biochar particle electrodes derived from waste iron bottle caps and sugarcane bagasse for the remediation of sodium dodecyl sulphate.

The present work demonstrates a novel strategy of synthesizing iron-biochar (Fe@BCSB) composite made with the waste iron bottle cap and sugar cane bagasse for implementation in the three-dimensional electro-Fenton (3DEF) process. The catalytic ability of the Fe@BCSB composite was explored to remediate the sodium dodecyl sulphate (SDS) surfactant from wastewater at neutral pH. At the optimum operating condition of Fe@BCSB dose of 1.0 g L-1, current density of 4.66 mA cm-2, and Na2SO4 dose of 50 mM, nearly 92.7 ± 3.1% of 20 mg L-1 of SDS abatement was attained during 120 min of electrolysis time. Moreover, the Fe@BCSB showed significant recyclability up to six cycles. Besides, other organics were successfully treated with more than 85% abatement efficiency in the proposed Fe@BCSB-supported 3DEF process. The total operating cost obtained during SDS treatment was around 0.31 US$ m-3 of wastewater. The phytotoxicity test revealed the positive impact of the 3DEF-treated effluent on the germination of the Vigna radiata. The electron paramagnetic resonance conveyed •OH as the prevailing reactive species for the oxidation of SDS in the 3DEF process. Further, about 81.3 ± 3.8% of SDS and 53.7 ± 4.1% of mineralization efficacy were acquired from the real institutional sewage.

[Changes of brain network in patients with insomnia following radiotherapy for cervical cancer and their correlation with IT-TMS treatment efficacy: a graph-theory analysis].

OBJECTIVE: To analyze the changes of brain small-world and node function network properties in patients with insomnia following radiotherapy for cervical cancer based on graph theory analysis and explore the correlation between functional networks and the clinical efficacy of individual-target transcranial magnetic stimulation (IT-TMS) for treatment of insomnia. METHODS: The resting state functional magnetic resonance imaging (rs-fMRI) data were collected from 30 patients with insomnia following radiotherapy for cervical cancer and 30 matched healthy individuals. All the patients received accelerated intelligent neuromodulation TMS therapy. Using graph theory analysis and GRETNA software, the functional connectivity matrices were constructed and the attribute features were extracted. The scores on the Pittsburgh Sleep Quality Index (PSQI), Insomnia Severity Index (ISI), Self-Rating Anxiety Scale (SAS), and Self-Rating Depression Scale (SDS) of the participants were collected before and after IT-TMS, and the correlation between improvement in insomnia and the functional network was investigated. RESULTS: The two groups matched for age, gender, and education level (P>0.05) showed significant differences in PSQI, ISI, SAS and SDS scores (P<0.05). Both groups showed attributes of the small-world network. Compared with the healthy individuals, the patients showed significantly decreased σ, EI, Cp and Lp (P<0.05) and increased Eg (P<0.05) at baseline, which, along with insomnia symptoms, were all improved after IT-TMS treatment. The patients showed reduced functional connections of the node network at follow-up compared with the baseline, and the low functional connectivity between the right insula and left superior frontal gyrus was correlated with the improvement of ISI scores. CONCLUSION: The patients with insomnia following radiotherapy for cervical cancer have impaired information integration ability of the brain network, IT-TMS can significantly improve insomnia symptoms by reducing the hyperconnectivity between the default mode network and the salience network.

Spatiotemporal-based automated inundation mapping of Ramsar wetlands using Google Earth Engine.

Wetlands are one of the most critical components of an ecosystem, supporting many ecological niches and a rich diversity of flora and fauna. The ecological significance of these sites makes it imperative to study the changes in their inundation extent and propose necessary measures for their conservation. This study analyzes all 64 Ramsar sites in China based on their inundation patterns using Landsat imagery from 1991 to 2020. Annual composites were generated using the short-wave infrared thresholding technique from June to September to create inundation maps. The analysis was carried out on each Ramsar site individually to account for its typical behavior due to regional geographical and climatic conditions. The results of the inundation analysis for each site were subjected to the Mann-Kendall test to determine their trends. The analysis showed that 8 sites exhibited a significantly decreasing trend, while 14 sites displayed a significantly increasing trend. The accuracy of the analysis ranged from a minimum of 72.0% for Hubei Wang Lake to a maximum of 98.0% for Zhangye Heihe Wetland National Nature Reserve. The average overall accuracy of the sites was found to be 90.0%. The findings emphasize the necessity for conservation strategies and policies for Ramsar sites.

Mapping and assessment of river water quality under varying hydro-climatic and pollution scenarios by integrating QUAL2K, GEFC, and GIS.

Water quality modelling has proved to be effective method for managing river water quality. But the most effective and comprehensive approach involving integration of river water quality simulation and pollution visualization with the objective of pollution reduction and maintenance of environmental flow strategies has gained less attention. Thus, the objective of this study was to employ an integrated approach for mapping and analysing river water quality under various hydro-climatic and pollution scenarios. Specifically, this approach involved the integration of a river water quality simulation model, QUAL2K, Global Environmental Flow Calculator (GEFC), and Geographical Information System (GIS) to develop water quality index (WQI) based map charts of water quality. The calibrated QUAL2K model was utilized to simulate WQI parameters including water temperature, pH, electrical conductivity, dissolved oxygen (DO), biological oxygen demand (BOD), nitrates (NO3), ammonia (NH4), and alkalinity. To analyse the WQI, the Weighted Arithmetic-Water Quality Index (WA-WQI) method was employed for various individual and combined pollution scenarios, environmental flow (Eflow), and climate change scenarios. The developed integrated approach was applied to the Bhadravati segment of Bhadra River, India. The findings revealed that the prevailing WQI status of the study stretch ranged from poor to unsuitable for drinking purposes. This deterioration can be attributed to the impact of both industrial and municipal effluents. By maintaining the effective Environmental Management Class (EMC) flow rates (class C flowrate of EMC (40.32 m3/s)) in conjunction with appropriate Pollution Reduction (PR) level (10% PR) at headwater and incoming drains, the stream self-purification capacity was enhanced resulting in the Bhadravati River stretch water quality transitioning to favourable water quality condition.

Effect of resveratrol and combination of resveratrol and donepezil on the expression of microglial cells and astrocytes in Wistar albino rats of colchicine-induced Alzheimer's disease.

Aim: The goal was to evaluate the effect of resveratrol (RS) and combination therapy of RS and donepezil (DPZ), on the numerical expression of microglial cells and astrocytes, in the frontal cortex, regions of the hippocampus in colchicine-induced Alzheimer's disease (AD) model. Methods: The study involved male albino Wistar rats of three months, age and consisted of 6 groups, with six animals each. The immunohistochemical staining with mouse monoclonal anti-human CD 68 and mouse monoclonal anti-GFAP was performed to assess the number of microglial cells and astrocytes, respectively. Results: AD group showed an increase in the number of microglia, and the numbers declined in the treatment groups, RS 10, RS 20, RS10/10 and DPZ + RS (p < 0.001). Astrocyte count was increased in the treatment groups in contrast to the AD group (p < 0.05). The DPZ + RS combination group revealed substantial elevation in the number of astrocytes and decreased microglial number among all the groups (p < 0.001). Conclusion: RS administration has diminished the microglial number and elevated the number of astrocytes. The elevated reactive astrocytes have decreased the microglial population. However, the limitation of our study is utilizing the colchicine for the induction of neurodegeneration. Using the transgenic models of AD may give a better insight into the pathogenesis and effect of RS. Another limitation of this study is the administration of RS and DPZ through different routes. The prospects of this research include studying the probiotic nature of RS and the effect of RS in other neurodegenerative disorders.

A Randomized, Single-Blind Study Comparing the Clinical Equivalence of Truglyde Fast® and Safil Quick® Polyglycolic Acid Fast-Absorbing Sutures for Episiotomy Repair Following Vaginal Delivery.

INTRODUCTION: Episiotomy, the deliberate surgical incision on the vaginal orifice during vaginal delivery, requires prompt repairing of the incised tissue. It may be associated with bleeding, infection, dehiscence, dyspareunia, short-term pain, and prolonged hospital stay. The outcome of surgery depends on the suture material and technique to repair the episiotomy. OBJECTIVE: We aim to subjectively assess perineal pain and maternal morbidity following episiotomy repair with Truglyde Fast® (Healthium Medtech Limited, Bengaluru, India) and Safil Quick® (B. Braun Medical Private Limited, Mumbai, India) polyglycolic acid fast-absorbing suture. MATERIALS AND METHODS: This multicentric, prospective, randomized (1:1), two-arm, parallel-group, single-blind study was started in August 2020 and completed in March 2021. Ninety-nine primiparous or multiparous eligible women requiring episiotomy were recruited to Truglyde Fast® (n=51) and Safil Quick® (n=48) groups. The primary outcome measure was post-episiotomy perineal pain to be assessed using a visual analog scale (VAS). Secondary endpoints included evaluation of local anesthesia (quantity), intraoperative suture handling, number of sutures utilized, time spent for episiotomy repair and complete healing, analgesic number and dosage, early and late wound complications, presence of residual suture and frequency of re-suturing, resumption of sexual activity and dyspareunia, and adverse events. The threshold to discriminate significant from non-significant outcomes was p<0.05. RESULTS: At all visits, a non-significant change in perineal pain was noted between Truglyde Fast® and Safil Quick® groups. A significant difference (p<0.05) in the number of sutures used and intraoperative handling characteristics was observed between thegroups. Results of other secondary endpoints showed non-significant differences. CONCLUSION: Truglyde Fast® and Safil Quick® polyglycolic acid fast-absorbing sutures are clinically equivalent. Both sutures are safe and effective for episiotomy repair following vaginal delivery with minimal perineal pain and risk of maternal morbidity.

Toxicity evaluation of a heavy-metal-polluted river: Pollution identification and bacterial community assessment.

The Salt River is an important urban river in Kaohsiung, Taiwan. In this study, the source identification and risk and toxicity assessment of the heavy-metal-contaminated sediments in the Salt River were investigated. The geo-accumulation index (Igeo), enrichment factor (EF), sediment quality guidelines (SQGs), potential ecological risk index (RI), pollution load index (PLI), and toxic units (TU) were applied to determine effects of heavy metals on microbial diversities and ecosystems. Results from the ecological and environmental risk assessment show that high concentrations of Zn, Cr, and Ni were detected in the midstream area and the sum of toxic units (ΣTUs) in the midstream (7.2-32.0) is higher than in the downstream (14.0-19.7) and upstream (9.2-17.1). It could be because of the continuous inputs of heavy-metal-contained wastewaters from adjacent industrial parks. Results also inferred that the detected heavy metals in the upstream residential and commercial areas were possibly caused by nearby vehicle emissions, non-point source pollution, and domestic wastewater discharges. Results of metagenomic assays show that the sediments contained significant microbial diversities. Metal-tolerant bacterial phyla (Proteobacteria: 24.4%-46.4%, Bacteroidetes: 1.3%-14.8%, and Actinobacteria: 2.3%-11.1%) and pathogenic bacterial phyla (Chlamydiae: 0.5%-37.6% and Chloroflexi: 5.8%-7.2%) with relatively high abundance were detected. Metal-tolerant bacteria would adsorb metals and cause the increased metal concentrations in sediments. Results indicate that the bacterial composition in sediment environments was affected by anthropogenic pollution and human activities and the heavy-metal-polluted ecosystem caused the variations in bacterial communities. PRACTITIONER POINTS: Microbial community in sediments is highly affected by heavy metal pollution. Wastewaters and vehicle traffic contribute to river sediments pollution by heavy metals. Proteobacteria, Bacteroidota, and Actinobacteria are dominant heavy-metal-tolerant bacterial phyla in sediments. Toxicity assessment is required to study risk levels of heavy-metal contained sediments.

A critical review on the vermicomposting of organic wastes as a strategy in circular bioeconomy: mechanism, performance, and future perspectives.

To meet the current need for sustainable development, vermicomposting (VC), a natural, eco-friendly, and cost-effective technology, can be a wise selection for the bioconversion of organic wastes into value-added by-products. However, no one has tried to establish the VC technology as an economically sustainable technology by exploring its linkage to circular bioeconomy. Even, no researcher has made any effort to explore the usability of the earthworms (EWs) as a protein supplement while assessing the economic perspectives of VC technology. Very few studies are available on the greenhouse gas (GHG) emission potential of VC technology. Still, the contribution of VC technology towards the non-carbon waste management policy is not yet explored. In the current review, a genuine effort has been made to inspect the contribution of VC technology towards the circular bioeconomy, along with evaluating its capability to bioremediate the organic wastes generated from domestic, industrial, and agricultural premises. The potential of the EWs as a protein source has also been explored to strengthen the contribution of VC technology towards the circular bioeconomy. Moreover, the linkage of the VC technology to the non-carbon waste management policy has been comprehensively demonstrated by highlighting its carbon sequestration and GHG emission potentials during the treatment of organic wastes. It has been observed that the cost of food production was reduced by 60-70% by replacing chemical fertilizers with vermicompost. The implication of the vermicompost significantly lessened the harvesting period of the crops, thereby helping the farmers attain higher profits by cultivating more crops in a single calendar year on the same plot. Furthermore, the vermicompost could hold the soil moisture for a long time, lessening the water demand up to 30-40%, which, in turn, reduced the frequency of irrigation. Also, the replacement of the chemical fertilizers with vermicompost resulted in a 23% increment in the grapes' yield, engendering an extra profit of up to 110000 rupees/ha. In Nepal, vermicompost has been produced at a cost of 15.68 rupees/kg, whereas it has been sold to the local market at a rate of 25 rupees/kg as organic manure, ensuring a net profit of 9.32 rupees/kg of vermicompost. EWs embraced 63% crude protein, 5-21% carbohydrates, 6-11% fat, 1476 kJ/100 g of metabolizable energy, and a wide range of minerals and vitamins. EWs also contained 4.11, 2.04, 4.43, 2.83, 1.47, and 6.26 g/kg (on protein basis) of leucine, isoleucine, tryptophan, arginine, histidine, and phenylalanine, respectively, enhancing the acceptability of the EW meal (EWM) as the protein supplement. The inclusion of 3 and 5% EWM in the diet of broiler pullets resulted in a 12.6 and 22.5% increase in their feed conversion ratio (FCR), respectively after one month. Similarly, when a 100% fish meal was substituted by 50% EWM and 50% fish meal, the FCR and growth rate of Parachanna obscura were increased substantially. The VC of maize crop residues mixed with pig manure, cow dung, and biochar, in the presence of Eisenia fetida EWs, yielded only 0.003-0.081, 0-0.17, and 130.40-189.10 g CO2-eq.kg-1 emissions of CO2, CH4, and N2O, respectively. Similarly, the VC of tomato stems and cow dung ensured 2.28 and 5.76 g CO2-eq.kg-1 CO2 emissions of CH4 and N2O, respectively. Additionally, the application of vermicompost at a rate of 5 t/ha improved the soil organic carbon proportion and aggravated carbon sequestration. The land application of vermicompost improved micro-aggregation and cut down the tillage, reducing GHG emissions and triggering carbon sequestration. The significant findings of the current review suggest that VC technology potentially contributes to the concept of circular bioeconomy, substantially negotiates potential GHG emissions, and complies with the non-carbon waste management policy, reinforcing its acceptability as an economically sound and environmentally benevolent organic waste bioremediation alternative.

Using innovative copper-loaded activated alumina (Cu/AA) as the catalyst for catalytic wet peroxidation (CWPO) of catechol.

In this study, copper-loaded activated alumina (Cu/AA) was synthesized and used for the CWPO of catechol (a representative refractory organic pollutant). Various characterization techniques were deployed to characterize the catalysts, e.g., activated alumina (AA), as well as pristine and spent 1% Cu/AA. The innovative 1% Cu/AA catalyst exhibited good thermal stability up to 1173 K with a marginal weight loss of 13%. The Cu species were well dispersed on the activated alumina framework with no significant cluster formation. Typically, the average copper particle size of 5 nm was dispersed on the AA framework. Catechol removal was observed to be 92% with 87% mineralization at optimized conditions (initial catechol concentration = 200 mg/L, catalyst dose of 1% Cu/AA = 2 g/L; temperature = 323 K; pH = 6; and H2O2/catechol stoichiometric ratio = 0.5). The mineralization of catechol was analyzed using mass spectroscopy, with the associated mechanism has been elucidated. Results of this study indicated that synthesized catalyst has phenomenal advantages in terms of simple separation and high removal efficiency of catechol, suggesting the feasibility of employing Cu/AA as the effective catalyst for the CWPO of catechol.

Vermifiltration: Strategies and techniques to enhance the organic and nutrient removal performance from wastewater.

The vermifiltration (VF) technology has gained significant attention as a green alternative for remediating domestic and industrial wastewater over the last few decades. Of late, the implementation of various modifications to the orthodox VF technology, including tweaks in the design and operation of the vermifilters, has been portrayed in the available literature. However, owing to the scatteredness of the available information, the knowledge regarding the execution of the modified vermifilters is still inadequate. Hence, an effort has been made to comprehensively overview the innovative strategies and techniques adopted to improve the organic and nutrient removal potential of the VFs from wastewater. In addition, future perspectives have been recognized to design more efficient and sustainable VFs. This review explores more of such novel tactics to improve the performance of the VF technology regarding organic and nutrient removal from wastewater. PRACTITIONER POINTS: Innovative strategies and techniques implemented to VF technology were comprehensively overviewed. Design modification and advantages of each innovation were highlighted. The pollutant removal performance of every modification was emphasized. Modified vermifilters were better than the conventional vermifilters in terms of organic and nutrient removal from the wastewater.

Comparison of GHG emissions from open field burning and anaerobic digestion of rice straw.

Rice straw (RS) has been burned in open fields in many countries, which is a growing environmental concern. Anaerobic digestion (AD) of RS is one of the sustainable alternatives to open burning of RS for converting the biomass into methane, carbon dioxide, and digestate for use as agricultural manure. The present study evaluates the RS generation, use patterns, and greenhouse gas emissions from open field burning and the AD of RS. Results indicate the AD of RS offers greater environmental benefits than that by RS open field burning. As a result of the open burning of RS in India emitted 592032.52 kg of CO2 equivalents/kg per year, whereas AD of RS contributes 122.86 kg of CO2 equivalents/kg per year. If RS were burned in an open field, its Global Warming Potential would be 493,873 times greater than that of AD. By minimizing GHG emissions and creating sustainable energy from waste, sustainable development, and circular economies are achieved.

[Thoracic paravertebral block improves the prognosis of patients undergoing lung cancer surgery].

OBJECTIVE: To explore the effect of thoracic paravertebral anesthesia (TPVB) on prognosis of patients undergoing resection of lung cancer. METHODS: This study was conducted among the patients undergoing surgical resection of primary lung cancer under general anesthesia or TPVB combined with general anesthesia (TPVB+GA) between January, 2017 and May, 2018.The patients were enrolled in TPVB+GA group and GA group (control group) using a propensity score matching (PSM) method at the ratio of 1:2 based on their baseline characteristics.The clinical parameters, 5-year overall survival (OS), progression-free survival (PFS) and intraoperative dosage of opioids were compared between the two groups to assess the impact of TPVB on prognosis of the patients. RESULTS: Forty-seven patients were enrolled in TPVB+GA group and 94 in the control group.Kaplan-Meier survival analysis showed a significantly prolonged PFS in the patients with TPVB+GA (log-rank P=0.034), with an odds ratio (OR) of 0.45(95%CI: 0.33-0.89).Consistently, univariate and multivariate Cox regression analyses identified TPVB as an independent protective prognostic factor for patients with lung cancer resection (P=0.002, OR=0.33, 95%CI: 0.16-0.66).Cox regression analyses indicated that a lower intraoperative dose of remifentanil was significantly correlated with a longer PFS of the patients following lung cancer resection (P=0.017, OR=0.47, 95%CI: 0.25-0.87).Chi-square test confirmed that TPVB, but not general anesthesia, significantly reduced intraoperative dose of remifentanil, indicating a possible synergistic effect of TPVB with opioids to affect the survival of the patients. CONCLUSION: TPVB can prolong the survival time and improve the prognosis of the patients undergoing surgical resection of lung cancer.

Toxicity determination, pollution source delineation, and microbial diversity evaluation of PAHs-contaminated sediments for an urban river.

The Feng-Sang River is a metropolitan river in Kaohsiung City, Taiwan. In this study, Feng-Sang River sediments were analyzed to investigate the distributions and sources of polycyclic aromatic hydrocarbons (PAHs). The Sediment Quality Guidelines (SQGs), potentially carcinogenic PAHs (TEQcarc ), and toxic equivalence quotient (TEQ) were applied to evaluate influences of PAHs on ecosystems and microbial diversities. Results indicate that PAHs concentrations varied between seasons and locations. The concentrations of ∑16 PAHs ranged from 73.6 to 603.8 ng/kg in dry seasons and from 2.3 to 199.3 ng/kg in wet seasons. This could be because of the flushing effect during wet seasons, which caused the movement and dilution of the PAH-contaminated sediments. Diagnostic ratio analysis infers that high PAHs levels were generated by combustion processes and vehicle traffic, and results from multivariate descriptive statistical analysis also demonstrate that the vehicular traffic pollution could be the major emission source of PAHs contamination. Comparisons of PAHs with SQGs indicate that PAHs concentrations in sediment were below the effects range low (ERL) values, and thus, the immediate threat to organisms might not be significant. The diagnostic ratio analyses are effective methods for PAH source appointment. The metagenomic assay results imply that sediments contained essential microbial species with eminent diversity. The detected PAH-degrading bacteria (Desulfatiglans, Dechloromonas, Sphingomonas, Methylobacterium, Rhodobacter, Clostridium, and Exiguobacterium) played a key role in PAHs biotransformation, and Dechloromonas and Rhodobacter had a higher relative abundance. Results of microbial diversity analyses indicate that the contaminated environment induced the changes of governing microbial groups in sediments. PRACTITIONER POINTS: Diagnostic ratio analyses are effective methods for PAHs source appointment. Microbial composition in sediments are highly affected by anthropogenic pollution. Combustion and vehicle traffic contribute to urban river sediments pollution by PAHs. Dechloromonas and Rhodobacter are dominant PAHs-degrading bacteria in sediments.

Access to Spiroindanolactones/lactams through an Aryne Insertion/Spirocyclization Strategy.

An efficient and metal-free strategy for the synthesis of spiro-fused indanolactones/lactams has been developed for the reaction of arynes with α-chloroacetyl lactones/lactams. This strategy provides access to spiroindanone derivatives via aryne insertion/spirocyclization.

Novel MSH6 mutation predicted metastasis in eyelid and periocular squamous cell carcinoma.

BACKGROUND: Our previous research revealed the relative local aggressiveness of eyelid and periocular squamous cell carcinoma (EPSCC), but its distinct genetic characteristics involved remain unknown. OBJECTIVES: We conducted this study based on next-generation sequencing to identify the genetic distinctiveness of EPSCC and damaging mutations for possible aetiology and poor prognosis. METHODS: We performed sequencing using a 556-gene panel (SmartOnco) in 48 EPSCCs. Cox hazards model was applied to explore mutated genes that increase the risk of metastasis and death. Pathogenesis of the mutations was predicted by sequence alignment algorithms. RESULTS: The most commonly mutated genes were KMT2C (N = 17, 35%), LRP1B (N = 14, 29%), KMT2D (N = 12, 25%), PTCH1 (N = 10, 21%) and TP53 (N = 10, 21%). DNA mismatch repair (MMR) genes (42%) like MSH6 (19%) and MLH3 (12%) were among the most frequently mutated genes. Cell cycle regulators including TP53 (21%) and CDKN2A (10%) were less frequently mutated than in other squamous cell carcinomas (SCCs). Ultraviolet exposure, MMR deficiency and ageing were the main aetiology. Of note, KMT2C has a deleterious mutation hotspot. Patients burdened with MSH6 mutation has a higher risk of overall metastasis (P = 0.045, HR = 5.165) and nodal metastasis (P = 0.022, HR = 14.038). Moreover, a hotspot mutation MSH6E52A brought an even higher risk of nodal metastasis (P = 0.011, HR = 18.745). CONCLUSIONS: EPSCCs displayed a unique mutation profile from cutaneous SCCs and mucosal SCCs. We have identified novel damaging mutations in epigenetic regulators like KMT2C boosted early onset of EPSCCs in addition to UVR, ageing or MMR deficiency. And malfunction of MMR genes worsened prognosis.

Hippocampus and its involvement in Alzheimer's disease: a review.

Hippocampus is the significant component of the limbic lobe, which is further subdivided into the dentate gyrus and parts of Cornu Ammonis. It is the crucial region for learning and memory; its sub-regions aid in the generation of episodic memory. However, the hippocampus is one of the brain areas affected by Alzheimer's (AD). In the early stages of AD, the hippocampus shows rapid loss of its tissue, which is associated with the functional disconnection with other parts of the brain. In the progression of AD, atrophy of medial temporal and hippocampal regions are the structural markers in magnetic resonance imaging (MRI). Lack of sirtuin (SIRT) expression in the hippocampal neurons will impair cognitive function, including recent memory and spatial learning. Proliferation, differentiation, and migrations are the steps involved in adult neurogenesis. The microglia in the hippocampal region are more immunologically active than the other regions of the brain. Intrinsic factors like hormones, glia, and vascular nourishment are instrumental in the neural stem cell (NSC) functions by maintaining the brain's microenvironment. Along with the intrinsic factors, many extrinsic factors like dietary intake and physical activity may also influence the NSCs. Hence, pro-neurogenic lifestyle could delay neurodegeneration.