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1.
Prikl Biokhim Mikrobiol ; 17(2): 238-40, 1981.
Artículo en Ruso | MEDLINE | ID: mdl-6787580

RESUMEN

The possibility of concentrating proteases from Streptomyces griseus by the ultrafiltration technique using Soviet membranes of the MB-I-IV type was explored. This method allowed a 3-10 fold reduction of the native solution of enzymes and their simultaneous purification from by-products.


Asunto(s)
Streptomyces griseus/enzimología , Péptido Hidrolasas/aislamiento & purificación , Ultrafiltración
2.
Prikl Biokhim Mikrobiol ; 15(5): 702-6, 1979.
Artículo en Ruso | MEDLINE | ID: mdl-42050

RESUMEN

From the culture liquid filtrate of Aspergillus awamori 78-2 a preparation of acid proteinase was isolated and its properties were described. This producer was shown to have a peculiar capacity of synthesizing under submerged conditions extracellular acid proteinase as the sole proteolytic enzyme. The preparation of acid proteinase showed a comparatively high hydrolyzing capacity. Under optimal conditions the enzyme degraded hemoglobin and albumin almost completely. The possibility of purifying and concentrating acid proteinase on the ion-exchange resin KB 51 X 2 was explored.


Asunto(s)
Aspergillus/enzimología , Péptido Hidrolasas/metabolismo , Cromatografía por Intercambio Iónico/métodos , Concentración de Iones de Hidrógeno , Cinética , Péptido Hidrolasas/aislamiento & purificación
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