RESUMEN
The possibility of concentrating proteases from Streptomyces griseus by the ultrafiltration technique using Soviet membranes of the MB-I-IV type was explored. This method allowed a 3-10 fold reduction of the native solution of enzymes and their simultaneous purification from by-products.
Asunto(s)
Streptomyces griseus/enzimología , Péptido Hidrolasas/aislamiento & purificación , UltrafiltraciónRESUMEN
From the culture liquid filtrate of Aspergillus awamori 78-2 a preparation of acid proteinase was isolated and its properties were described. This producer was shown to have a peculiar capacity of synthesizing under submerged conditions extracellular acid proteinase as the sole proteolytic enzyme. The preparation of acid proteinase showed a comparatively high hydrolyzing capacity. Under optimal conditions the enzyme degraded hemoglobin and albumin almost completely. The possibility of purifying and concentrating acid proteinase on the ion-exchange resin KB 51 X 2 was explored.