RESUMEN
We studied the kinetics of liver aldehyde dehydrogenase in rats with cold injury. Low-temperature exposure was followed by a decrease in activity and catalytic efficiency of aldehyde dehydrogenase in rat liver mitochondria. Atypical changes in kinetic characteristics of aldehyde dehydrogenase were found in the cytoplasmic fraction during cold injury.
Asunto(s)
Aldehído Deshidrogenasa/metabolismo , Frío , Hígado/enzimología , Animales , Cinética , Ratas , Ratas WistarRESUMEN
Lactate dehydrogenase activity, crystallogenic and initiator characteristics of biological substrates were evaluated by enzymological and crystalloscopic analysis of rat serum and urine. Changes in these characteristics of biological media in combined thermal injury were shown. This approach is effective for evaluation of the metabolic status in rats with experimental burn disease.
Asunto(s)
Quemaduras/sangre , Quemaduras/orina , Calor , L-Lactato Deshidrogenasa/sangre , L-Lactato Deshidrogenasa/orina , Animales , Quemaduras/metabolismo , Cristalografía , Eritrocitos/enzimología , Eritrocitos/metabolismo , L-Lactato Deshidrogenasa/metabolismo , Ratas , Ratas WistarRESUMEN
We studied enzyme systems (lactate dehydrogenase) of mitochondria in cerebral nerve cells in experimental encephalopathy developing after thermal injury. In animals receiving neuromedin at the early terms after injury, the ratio of forward to reverse lactate dehydrogenase reactions significantly increased over the first day after injury and returned to normal on day 7.