RESUMEN
The receptors and signaling pathways for nongenomic effects of aldosterone (Aldo) on the proximal Na+/H+ exchanger are still unknown; therefore, the aim of this study was to investigate the mineralocorticoid receptor (MR) and/or glucocorticoid receptor (GR) participation in rapid Aldo effects on NHE1 (basolateral Na+/H+ exchanger isoform) and cytosolic calcium concentration ([Ca2+]i). In addition, phospholipase C (PLC), protein kinase C (PKC), and mitogen-activated protein kinase kinase (MEK) involvement in signaling pathways of such effects was evaluated, using immortalized proximal tubule cells of rat (IRPTC) as an experimental model. MR and GR expression was investigated using reverse transcription polymerase chain reaction and immunoblotting. The intracellular pH recovery rate (after acid loading) and [Ca2+]i were determined by the probes BCECF-AM and FURA 2-AM, respectively. Aldo (10-12â¯M) promoted a moderate increase in [Ca2+]i and stimulation of NHE1, whereas Aldo (10-6â¯M) greatly increased the [Ca2+]i, but inhibited the NHE1. BAPTA-AM (a calcium chelator), GR antagonism and inhibition of PLC, PKC and MEK pathway abolished the biphasic and dose-dependent effect of Aldo on NHE1 and decreased the [Ca2+]i; whereas MR do not appear to participate in this rapid signaling in IRPTC cells. The reduction of GR content, by gene silencing, abolished the Aldo effect on NHE1, in low concentration, confirming the importance of this receptor in the rapid modulation of proximal sodium and hydrogen transports.
Asunto(s)
Aldosterona/farmacología , Regulación de la Expresión Génica/efectos de los fármacos , Túbulos Renales Proximales/metabolismo , MAP Quinasa Quinasa 1/metabolismo , Proteína Quinasa C/metabolismo , Intercambiador 1 de Sodio-Hidrógeno/metabolismo , Fosfolipasas de Tipo C/metabolismo , Animales , Calcio/metabolismo , Células Cultivadas , Túbulos Renales Proximales/efectos de los fármacos , MAP Quinasa Quinasa 1/genética , Proteína Quinasa C/genética , Ratas , Receptores de Glucocorticoides/metabolismo , Transducción de Señal/efectos de los fármacos , Intercambiador 1 de Sodio-Hidrógeno/genética , Fosfolipasas de Tipo C/genéticaRESUMEN
Physiological concentrations of angiotensin II (ANG II) upregulate the activity of Na(+)/H(+) exchanger isoform 3 (NHE3) in the renal proximal tubule through activation of the ANG II type I (AT1) receptor/G protein-coupled signaling. This effect is key for maintenance of extracellular fluid volume homeostasis and blood pressure. Recent findings have shown that selective activation of the beta-arrestin-biased AT1 receptor signaling pathway induces diuresis and natriuresis independent of G protein-mediated signaling. This study tested the hypothesis that activation of this AT1 receptor/beta-arrestin signaling inhibits NHE3 activity in proximal tubule. To this end, we determined the effects of the compound TRV120023, which binds to the AT1R, blocks G-protein coupling, and stimulates beta-arrestin signaling on NHE3 function in vivo and in vitro. NHE3 activity was measured in both native proximal tubules, by stationary microperfusion, and in opossum proximal tubule (OKP) cells, by Na(+)-dependent intracellular pH recovery. We found that 10(-7) M TRV120023 remarkably inhibited proximal tubule NHE3 activity both in vivo and in vitro. Additionally, stimulation of NHE3 by ANG II was completely suppressed by TRV120023 both in vivo as well as in vitro. Inhibition of NHE3 activity by TRV120023 was associated with a decrease in NHE3 surface expression in OKP cells and with a redistribution from the body to the base of the microvilli in the rat proximal tubule. These findings indicate that biased signaling of the beta-arrestin pathway through the AT1 receptor inhibits NHE3 activity in the proximal tubule at least in part due to changes in NHE3 subcellular localization.
Asunto(s)
Arrestinas/metabolismo , Túbulos Renales Proximales/citología , Receptor de Angiotensina Tipo 1/fisiología , Intercambiadores de Sodio-Hidrógeno/metabolismo , Bloqueadores del Receptor Tipo 1 de Angiotensina II/farmacología , Animales , Humanos , Concentración de Iones de Hidrógeno , Túbulos Renales Proximales/fisiología , Masculino , Oligopéptidos/farmacología , Ratas , Ratas Sprague-Dawley , Transducción de Señal , Sodio/metabolismo , Intercambiador 3 de Sodio-Hidrógeno , Intercambiadores de Sodio-Hidrógeno/genética , beta-ArrestinasRESUMEN
Angiotensin II (Ang II) exerts an acute bimodal effect on proximal tubule NHE3: while low doses stimulate the exchanger, high doses inhibit it. In the present study, we have investigated the chronic effects of Ang II on NHE3 expression and transcriptional regulation. Treatment of a tubular epithelial cell line, OKP, with Ang II 10(-11)M significantly increased NHE protein expression and mRNA levels, without evidence of bimodal effect. No change in mRNA half-life was detected, but transient transfection studies showed a significant increase in NHE3 promoter activity. Binding sites for Sp1/Egr-1 and AP2 transcription factors of the NHE3 proximal promoter were mutated and we observed that the Sp1/Egr-1 binding site integrity is necessary for Ang II stimulatory effects. Inhibition of cytochrome P450, PI3K, PKA and MAPK pathways prevented the Ang II stimulatory effect on the NHE3 promoter activity. Taking all the results together, our data reveal that chronic Ang II treatment exerts a stimulatory effect on NHE3 expression and promoter activity. The Ang II up-regulation of the NHE3 promoter activity appears to involve the Sp1/Egr-1 binding site and the interplay of several intracellular signaling pathways.
Asunto(s)
Angiotensina II/farmacología , Células Epiteliales/efectos de los fármacos , Riñón/efectos de los fármacos , Intercambiadores de Sodio-Hidrógeno/genética , Transcripción Genética/efectos de los fármacos , Activación Transcripcional , Animales , Sitios de Unión , Línea Celular , Proteína 1 de la Respuesta de Crecimiento Precoz/metabolismo , Células Epiteliales/metabolismo , Riñón/citología , Riñón/metabolismo , Regiones Promotoras Genéticas/efectos de los fármacos , Intercambiador 3 de Sodio-Hidrógeno , Factor de Transcripción Sp1/metabolismoRESUMEN
BACKGROUND: The diagnosis of Adult T-cell leukemia/lymphoma ATLL subtypes in human T-lymphotropic virus type I (HTLV-I) carriers based in morphology and immunophenotype of lymphocytes can be challenger. We propose that polymerase chain reaction (PCR) amplification of the rearranged TCR gene in HTLV-I healthy carriers would be a convenient method for establishing the nature of the circulating T lymphocytes in asymptomatic HTLV-I carriers, presenting only mild and inconclusive signals of deviation from normality. METHODS: Using PCR, we analyzed the genetic recombination pattern of the T-cell beta-chain receptor gene (TCR-beta) in order to identify clonal expansion of peripheral blood T lymphocytes in 17 HTLV-I-positive healthy carriers and in nine normal HTLV-I-negative blood donors. To evaluate the performance of PCR in detection of clonality, we also analyzed 18 patients with post-thymic/mature T-cell malignancies presenting circulating abnormal lymphocytes. RESULTS: Seven of the 17 HTLV-I positive individuals presented circulating abnormal lymphocytes; monoclonal or oligoclonal expansion of T-cells was detected in five of the 17 HTLV-I-positive individuals, all of them presenting abnormal lymphocytes. Clonal expansion was not detected in any of the negative controls or in any of the 12 remaining healthy carriers. All patients in the positive control group tested positive by PCR and Southern blots. Southern blots were negative for all 17 healthy carriers. CONCLUSIONS: PCR amplification of segments of rearranged TCR-beta is reliable for allowing early detection of small populations of clonal T cells in blood samples from asymptomatic HTLV-I carriers, providing an additional alert in the follow-up of carriers with abnormal circulating lymphocytes.
Asunto(s)
Portador Sano/diagnóstico , Reordenamiento Génico de la Cadena beta de los Receptores de Antígenos de los Linfocitos T/genética , Infecciones por HTLV-I/diagnóstico , Infecciones por HTLV-I/inmunología , Linfocitos T/patología , Adulto , Southern Blotting , Portador Sano/inmunología , Estudios de Casos y Controles , Proliferación Celular , Células Clonales , Femenino , Humanos , Leucemia-Linfoma de Células T del Adulto/inmunología , Leucemia-Linfoma de Células T del Adulto/prevención & control , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Linfocitos T/inmunologíaRESUMEN
BACKGROUND: The expression of glucocorticoid-receptor (GR) seems to be a key mechanism in the regulation of glucocorticoid (GC) sensitivity and is potentially involved in cases of GC resistance or hypersensitivity. The aim of this study is to describe a method for quantitation of GR alpha isoform (GRalpha) expression using real-time PCR (qrt-PCR) with analytical capabilities to monitor patients, offering standard-curve reproducibility as well as intra- and inter-assay precision. RESULTS: Standard-curves were constructed by employing standardized Jurkat cell culture procedures, both for GRalpha and BCR (breakpoint cluster region), as a normalizing gene. We evaluated standard-curves using five different sets of cell culture passages, RNA extraction, reverse transcription, and qrt-PCR quantification. Intra-assay precision was evaluated using 12 replicates of each gene, for 2 patients, in a single experiment. Inter-assay precision was evaluated on 8 experiments, using duplicate tests of each gene for two patients. Standard-curves were reproducible, with CV (coefficient of variation) of less than 11%, and Pearson correlation coefficients above 0,990 for most comparisons. Intra-assay and inter-assay were 2% and 7%, respectively. CONCLUSION: This is the first method for quantitation of GRalpha expression with technical characteristics that permit patient monitoring, in a fast, simple and robust way.
Asunto(s)
Reacción en Cadena de la Polimerasa/métodos , Receptores de Glucocorticoides/genética , Adulto , Niño , Femenino , Humanos , Células Jurkat , Masculino , Reacción en Cadena de la Polimerasa/normas , ARN Mensajero/análisis , ARN Mensajero/metabolismo , Receptores de Glucocorticoides/biosíntesis , Estándares de Referencia , Reproducibilidad de los ResultadosRESUMEN
BACKGROUND: Acyclovir (ACY) is a useful therapeutic agent for the systemic treatment of herpes virus infection. An increase in urinary phosphate excretion and polyuria has been described. The objective of this study was to analyze the exact mechanism of the urinary-concentrating dysfunction and the increase in phosphaturia associated with ACY. METHODS: We first analyzed 7 (adult and pediatric) non-AIDS cases of encephalitis receiving 15 mg/kg bw/d of intravenous ACY. Fractional phosphate and sodium excretion, urinary potassium volume, and plasma phosphate concentrations were analyzed. Additional studies in rats treated with intraperitoneal ACY (100 mg/kg bw) were also conducted. Animals were maintained in metabolic cages and 24-hour urine samples were collected to measure volume, osmolality, and sodium/potassium/phosphate excretion. Treated rats were also evaluated after 24 hours and 48 hours of water deprivation. Northern hybridization and semiquantitative immunoblotting were performed to evaluate (in both control and treated animals) expression of the cotransporters Na-Pi type IIa (Na-Pi-IIa) and Na-K-2Cl (NKCC2). Semiquantitative immunoblotting was carried out in the kidneys of ACY rats and control rats in order to analyze aquaporin 2 (AQP2) protein expression. RESULTS: Patients started on ACY developed polyuria and hyperphosphatemia after 48 hours. In rats, ACY-induced hyperphosphaturia and hypophosphatemia were accompanied by increased excretion of sodium, potassium, and magnesium, increased urine output, lower urinary osmolality, and a partial urinary concentrating defect. Concurrent downregulation of Na-Pi-IIa and NKCC2 expression was observed. There was also a decrease in medullar expression of the AQP2 collecting duct water channel. CONCLUSION: Downregulation of Na-Pi-IIa appears to play a crucial role in the downregulation of ACY-induced hyperphosphaturia. The accompanying polyuria and urinary-concentrating defect can in part be explained by the downregulation of NKCC2 and AQP2.
Asunto(s)
Aciclovir/efectos adversos , Antivirales/efectos adversos , Acuaporinas/metabolismo , Hipofosfatemia/metabolismo , Poliuria/metabolismo , Simportadores de Cloruro de Sodio-Potasio/metabolismo , Adulto , Animales , Acuaporina 2 , Acuaporinas/genética , Calcio/sangre , Niño , Regulación hacia Abajo , Expresión Génica/efectos de los fármacos , Humanos , Hipofosfatemia/inducido químicamente , Hipofosfatemia/fisiopatología , Capacidad de Concentración Renal/efectos de los fármacos , Túbulos Renales Proximales/metabolismo , Magnesio/sangre , Masculino , Concentración Osmolar , Fosfatos/sangre , Fosfatos/orina , Poliuria/inducido químicamente , Poliuria/fisiopatología , Potasio/metabolismo , ARN Mensajero/metabolismo , Ratas , Ratas Wistar , Sodio/metabolismo , Simportadores de Cloruro de Sodio-Potasio/genética , Miembro 1 de la Familia de Transportadores de Soluto 12 , Orina , Agua/metabolismoRESUMEN
Isolated kidney mitochondria swell when incubated in hyposmotic solutions containing K+ salts in a manner inhibited by ATP, ADP, 5-hydroxydecanoate, and glibenclamide and stimulated by GTP and diazoxide. These results suggest the existence of ATP-sensitive K+ channels in these mitochondria, similar to those previously described in heart, liver, and brain. Renal mitochondrial ATP-sensitive K+ uptake rates are approximately 140 nmol.min-1.mg protein-1. This K+ transport results in a slight increase in respiration and decrease in the inner membrane potential. In addition, the activation of ATP-inhibited K+ uptake using diazoxide leads to a decrease of ATP hydrolysis through the reverse activity of the F0F1 ATP synthase when respiration is inhibited. In conclusion, we characterize an ATP-sensitive K+ transport pathway in kidney mitochondria that affects volume, respiration, and membrane potential and may have a role in the prevention of mitochondrial ATP hydrolysis.
Asunto(s)
Adenosina Trifosfato/farmacología , Riñón/metabolismo , Mitocondrias/metabolismo , Canales de Potasio/metabolismo , Animales , Transporte Biológico/efectos de los fármacos , Transporte Biológico/fisiología , Respiración de la Célula/fisiología , Precondicionamiento Isquémico , Masculino , Potenciales de la Membrana/fisiología , Dilatación Mitocondrial/efectos de los fármacos , Dilatación Mitocondrial/fisiología , Potasio/metabolismo , ATPasas de Translocación de Protón/metabolismo , Ratas , Ratas WistarRESUMEN
It is well established that after removal of renal mass, the remaining tissue undergoes compensatory growth. Several laboratories have reported that the activity of the apical membrane Na+ - H+ exchanger (NHE3) is increased after a reduction in renal mass. These studies were designed to determine whether NHE3 expression is altered early after loss of renal mass and to investigate the possible role of NHE3 activation in the compensatory tissue growth response. Experiments were performed in young male Wistar rats submitted to left nephrectomy or sham operation. At either 4 or 24 h after the surgery, the right kidney from each animal was removed and weighed. Significant increases in the wet weight of the remaining kidney were only observed 24 h after uninephrectomy (UNX). Western blot analysis of brush-border membranes and Northern blot analysis of cortex RNA showed that NHE3 protein abundance and NHE3 mRNA were greatly enhanced 4 and 24 h after UNX in relation to the sham kidney. To identify which growth pattern was mostly responsible for the enlargement of the remained kidney in our experimental models, we measured 5-bromo-2-deoxyuridine incorporation (BrdU) and protein-to-DNA ratio (protein/DNA ratio). The number of BrdU-positive nuclei increased and protein/DNA ratio slightly decreased, indicating that a hyperplastic response was the main component involved in the early compensatory renal growth in our animals. BrdU incorporation and protein/DNA were also assessed in rats treated with S3226, a selective blocker of NHE3. Neither the number of BrdU-positive nuclei nor the protein/DNA ratio was significantly altered 4 and 24 h after UNX in rats treated with S3226. In conclusion, UNX induced an upregulation of NHE3, which was evidenced at both functional and expression levels. The compensatory growth response in young UNX rats could be blocked by inhibiting NHE3 activity, suggesting that NHE3 activation may result in a facilitator state for the cell growth response in the renal proximal tubule.