Analysis of ovary-specific genes in relation to egg maturation and female nutritional condition in the mosquitoes Georgecraigius atropalpus and Aedes aegypti (Diptera: Culicidae).

Analysis of the reproductive physiology of anautogenous mosquitoes at the molecular level is complicated by the simultaneity of ovarian maturation and the digestion of a blood meal. In contrast to anautogenous mosquitoes, autogenous female mosquitoes can acquire greater nutrient stores as larvae and exhibit higher ovarian production of ecdysteroids at adult eclosion. These features essentially replace the role of a blood meal in provisioning the first batch of eggs and initiating egg development. To gain insight into the process of ovary maturation we first performed a transcript analysis of the obligatory autogenous mosquito Georgecraigius atropalpus (formerly Ochlerotatus atropalpus). We identified ESTs using suppressive subtractive hybridization (SSH) of transcripts from ovaries at critical times during oogenesis in the absence of blood digestion. Preliminary expression studies of genes such as apolipophorin III (APO) and oxysterol binding protein (OSBP) suggested these genes might be cued to female nutritional status. We then applied our findings to the medically important anautogenous mosquito Aedes aegypti. RNAi-based analyses of these genes in Ae. aegypti revealed a reduction in APO transcripts leads to reduced lipid levels in carcass and ovaries and that OSBP may play a role in overall lipid and sterol homeostasis. In addition to expanding our understanding of mosquito ovarian development, the continued use of a comparative approach between autogenous and anautogenous species may provide novel intervention points for the regulation of mosquito egg production.

Epidemiology of injuries requiring surgery among high school athletes in the United States, 2005 to 2010.

BACKGROUND: The proportion of high school sports-related injuries requiring surgery, which pose monetary and time loss burdens, has significantly increased during the last decade. The objective was to investigate the epidemiology of high school athletic injuries requiring surgery. METHODS: High school sports-related injury data were collected for nine sports from 2005 to 2010 from 100 nationally representative US high schools. RESULTS: Athletes sustained 1,380 injuries requiring surgery for a rate of 1.45 injuries per 10,000 athlete exposures. Boys' football had the highest injury rate (2.52) followed by boys' wrestling (1.64). Among gender comparable sports, girls' sports has a higher injury rate (1.20) than boys' (0.94) (rate ratio, 1.28; 95% confidence interval, 1.08-1.51; p=0.004). The rate of injuries was higher in competition (3.23) than practice (0.79) (rate ratio, 4.08; 95% confidence interval, 3.67-4.55; p<0.001) overall and in each sport. Commonly injured body sites were the knee (49.4%), head/face/mouth (9.7%), and shoulder (8.7%). Common diagnoses were complete ligament strain (32.1%) and fracture (26.4%). Nearly half (48.0%) resulted in medical disqualification for the season. CONCLUSIONS: Rates and patterns of injuries requiring surgery differ by sport, type of exposure, and gender. Future studies should identify sport-specific risk factors to drive effective interventions to decrease the incidence and severity of such injuries.

An epidemiologic comparison of high school sports injuries sustained in practice and competition.

CONTEXT: More than 7 million US high school students play sports. OBJECTIVE: To compare practice and competition injury rates and patterns in 5 boys' sports (football, soccer, basketball, wrestling, and baseball) and 4 girls' sports (soccer, volleyball, basketball, and softball) during the 2005-2006 school year. DESIGN: Prospective injury surveillance study. SETTING: Injury data were collected from 100 nationally representative United States high schools via High School RIO (Reporting Information Online). PATIENTS OR OTHER PARTICIPANTS: Athletes from participating high schools injured while participating in a school-sanctioned practice or competition in one of the above sports. MAIN OUTCOME MEASURE(S): Practice and competition injury rates, body site, diagnosis, and severity. RESULTS: High school athletes participating in these 9 sports at participating schools sustained 4350 injuries during the 2005-2006 school year, which corresponds to an estimated 1 442 533 injuries nationally. The rate of injury per 1000 athlete-exposures was higher in competition (4.63) than in practice (1.69) (rate ratio [RR] = 2.73, 95% confidence interval [CI] = 2.58, 2.90). Of all sports, football had the highest competition (12.09) and practice (2.54) injury rates per 1000 athlete-exposures. Compared with injuries sustained during practice, higher proportions of competition injuries were head/face/neck injuries (proportion ratio [PR] = 1.61, 95% CI = 1.34, 1.94), particularly in boys' soccer (PR = 7.74, 95% CI = 2.53, 23.65) and girls' basketball (PR = 6.03, 95% CI = 2.39, 15.22). Competition injuries were more likely to be concussions (PR = 2.02, 95% CI = 1.56, 2.62), especially in boys' soccer (PR = 6.94, 95% CI = 2.01, 23.95) and girls' basketball (PR = 5.83, 95% CI = 2.06, 16.49). Higher proportions of competition injuries caused the athlete to miss more than 3 weeks of play (PR = 1.28, 95% CI = 1.08, 1.52), particularly in baseball (PR = 3.47, 95% CI = 1.48, 8.11) and volleyball (PR = 2.88, 95% CI = 1.01, 8.24). CONCLUSIONS: Rates and patterns of high school sport injuries differed between practice and competition. Providing athletic trainers with this information is a crucial step in developing the targeted, evidence-based interventions required to effectively reduce injury rates among the millions of high school student-athletes.