RESUMEN
Parkinson's disease is characterized by a continuous spectrum of varying severity. The treatment is driven by new and sometimes highly complex therapeutic procedures. These two aspects are responsible for the blurred dividing line between outpatient and inpatient care. The aim of this article is to define criteria that should help determine the indication for inpatient or outpatient treatment. We introduce quality requirements that have already been taken into account in part in therapy modalities such as Parkinson complex treatment. The decision on the appropriate form of care affects the medical freedom of therapy, which must reconcile the legitimate interest of patients to receive optimal care with the given economic conditions. Our aim is to provide guidance on decisions on the best form of treatment in the context of changing framework conditions in the health sector.
Asunto(s)
Atención Ambulatoria , Pacientes Internos , Enfermedad de Parkinson/terapia , Manejo de Atención al Paciente , Hospitalización , HumanosRESUMEN
The capability of the adult brain to generate new hippocampal neurons after brain insults like stroke is decreasing during the aging process. Recent evidence further indicates that the proliferative properties of the precursor cells change in the aged brain. We therefore analyzed the early proliferative response of distinct precursor cell populations in the subgranular zone of the dentate gyrus in 3 and 16 months old transgenic nestin-green-fluorescent protein mice 4 days after ischemic cortical infarcts. A detailed immunocytochemical analysis of proliferating precursors revealed a significant infarct-induced activation of the earliest radial glia-like precursor cells (type 1 cells) and the more differentiated precursor cell subtypes (type 2b cells) in young mice. In contrast the proliferation of early neuronal precursor cells (type 2a cells) was stimulated in the aged brain. Additional long-term experiments further demonstrated that this differential proliferative response of distinct precursor cells is associated with an enhanced number of newborn neurons in the young DG after stroke whereas this increase in neurogenesis was absent in the aged brain. However, our study demonstrates that even precursor cells in the aged hippocampus possess the ability to respond to remote cortical infarcts.
Asunto(s)
Envejecimiento/patología , Accidente Cerebrovascular/patología , Animales , Proliferación Celular , Supervivencia Celular , Infarto Cerebral/patología , Giro Dentado/patología , Genes Reporteros , Proteínas Fluorescentes Verdes/genética , Proteínas de Filamentos Intermediarios/genética , Masculino , Ratones , Ratones Transgénicos , Proteínas del Tejido Nervioso/genética , Nestina , Células-Madre Neurales/patología , Neurogénesis , Neuroglía/patología , Neuronas/patologíaRESUMEN
Recent studies with functional magnetic brain imaging showed different task-related patterns of brain activation and deactivation in aged as compared to young healthy subjects. We hypothesized that these changes of brain activation patterns might be due to age-dependent changes of neuronal excitability. Therefore, we experimentally studied the functional cortical inhibition by paired pulse stimulation in brain slices of young adult (3 months), aged adult (24 months) and old (36 months) male rats. Field potentials were evoked by application of double pulses at layer VI/white matter and recorded in layer II/III. We also analyzed the regional distribution of five major gamma-aminobutyric acid A (GABA(A)) receptor subunits (alpha1, alpha2, alpha3, alpha5, and gamma2) by immunohistochemistry. A reduced functional inhibition in aged as compared to young animals associated with an altered composition of GABA(A)-receptors, especially a reduction of subunit alpha5 in aged animals, was shown. The present study suggests that the age-dependent functional activation patterns and possibly also the cognitive and motor abilities are at least partially modulated by an age-dependent alteration of functional inhibition in the neocortex.
Asunto(s)
Envejecimiento/fisiología , Encéfalo/fisiología , Inhibición Neural/fisiología , Animales , Potenciales Evocados , Inmunohistoquímica , Técnicas In Vitro , Masculino , Microelectrodos , Plasticidad Neuronal/fisiología , Lóbulo Parietal/fisiología , Ratas , Receptores de GABA-A/metabolismoRESUMEN
During sensorimotor recovery following stroke ipsi- and contralesional alterations in brain function have been characterized in patients as well as animal models of focal ischemia, but the contribution of these bilateral processes to the functional improvement is only poorly understood. Here we examined the role of the homotopic contralateral cortex for sensorimotor recovery after focal ischemic infarcts at different time periods after the insult. One group of animals received a unilateral single photothrombotic infarct in the forelimb sensorimotor cortex, while four additional groups received a second lesion in the contralateral homotopic cortex either immediately or 2 days, 7 days, or 10 days after the first infarct. The time course of functional recovery of the impaired forelimbs was assessed using different sensorimotor scores: forelimb-activity during exploratory behavior and frequency of forelimb-sliding in the glass cylinder as well as forelimb misplacement during grid walking. Focal infarcts in the forelimb sensorimotor cortex area significantly impaired the function of the contralateral forelimb in these different behavioral tests. The subsequent damage of the contralateral homotopic forelimb sensorimotor cortex only affected the forelimb opposite to the new lesion but did not reinstate the original deficit. The time course of sensorimotor recovery after bilateral sequential cortical infarcts did not significantly differ from animals with unilateral single lesions. These data indicate that following small ischemic cortical infarcts in the forelimb sensorimotor cortex the contralateral cortex homotopic to the lesion plays only a minor role for functional recovery.
Asunto(s)
Infarto Cerebral/fisiopatología , Miembro Anterior/fisiopatología , Lateralidad Funcional/fisiología , Recuperación de la Función/fisiología , Corteza Somatosensorial/fisiopatología , Animales , Conducta Animal , Infarto Cerebral/metabolismo , Infarto Cerebral/patología , Miembro Anterior/inervación , Masculino , Actividad Motora/fisiología , Desempeño Psicomotor/fisiología , Ratas , Factores de TiempoRESUMEN
Stimulation of cell proliferation and neurogenesis in the adult dentate gyrus has been observed after focal and global brain ischemia but only little is known about the underlying mechanisms. We here analyzed neurogenesis in the dentate gyrus after small cortical infarcts leaving the hippocampal formation and subcortical regions intact. Using the photothrombosis model in adult rats, focal ischemic infarcts were induced in different cortical areas (sensorimotor forelimb and hindlimb cortex) and proliferating cells were labeled at days 3-14 after infarct induction with bromodeoxyuridine. At 2, 4, and 10 weeks after ischemia, immunocytochemistry was performed with immature neuronal (doublecortin), mature neuronal (neuronal nuclei antigen) and glial (calcium-binding protein beta S100beta) markers. When compared with sham-operated controls, animals with infarcts in the forelimb as well as hindlimb cortex revealed an increase in survival of newborn progenitor cells at four and 10 weeks after the insult with predominance at the ipsilateral side. Triple immunofluorescence and confocal laser scanning microscopy revealed an increase in neurogenesis in all groups that was more pronounced 10 weeks after the infarct. Application of the N-methyl-D-aspartate (NMDA)-receptor antagonist MK-801 during lesion induction significantly enhanced neurogenesis in the dentate gyrus. An even stronger increase in newborn neurons was observed after anti-inflammatory treatment with indomethacine during the first 16 days of the experiment. The present study demonstrates that small cortical infarcts leaving subcortical structures intact increase neurogenesis in the dentate gyrus and that these processes can be stimulated by N-methyl-D-aspartate receptor blockade and anti-inflammatory treatment.
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Antiinflamatorios/farmacología , Infarto Cerebral/patología , Giro Dentado/efectos de los fármacos , Giro Dentado/patología , Antagonistas de Aminoácidos Excitadores/farmacología , Regeneración Nerviosa/efectos de los fármacos , Neuronas/patología , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Animales , Antimetabolitos , Bromodesoxiuridina , Supervivencia Celular/efectos de los fármacos , Maleato de Dizocilpina/farmacología , Proteína Doblecortina , Miembro Posterior/inervación , Inmunohistoquímica , Indometacina/farmacología , Masculino , Corteza Motora/efectos de los fármacos , Corteza Motora/patología , Vías Nerviosas/patología , Neuronas/efectos de los fármacos , Ratas , Ratas Wistar , Proteínas S100/metabolismo , Corteza Somatosensorial/efectos de los fármacos , Corteza Somatosensorial/patología , Trombosis/patologíaRESUMEN
Spreading depression (SD) is characterized by a transient breakdown of neuronal function concomitant with a massive failure of ion homeostasis. It is a phenomenon that can be induced in neocortical tissue by raising excitability, e.g. injection of K(+), application of glutamatergic agonists, or blocking Na(+)/K(+) ATPase. Here we report a novel method of SD induction using minimal disinhibition with application of low concentrations (5 microM) of the GABA(A) receptor blocker bicuculline. This procedure-while subthreshold for epileptiform activity-readily induced spontaneous SDs in native rat neocortical slices, accompanied by typical depolarizations of neurons and glial cells. In contrast, in human neocortical preparations obtained from epilepsy surgery, in approximately 20% of the slices spontaneous epileptiform activity appeared with this bicuculline dosage without SDs. Raising the concentration of bicuculline to an epileptogenic dose (10 microM) in human tissue also resulted in the generation of epileptiform activity only. Likewise, in slices from pilocarpine-treated, chronically epileptic rats, bicuculline also only induced epileptiform activity without eliciting SDs. The experiments indicate that chronic epilepsy causes a differential sensitivity to partial GABA(A) receptor blockade with regard to induction of SD.
Asunto(s)
Depresión de Propagación Cortical/fisiología , Epilepsia/fisiopatología , Neocórtex/fisiología , 2-Amino-5-fosfonovalerato/farmacología , Animales , Bicuculina/farmacología , Enfermedad Crónica , Relación Dosis-Respuesta a Droga , Estimulación Eléctrica , Epilepsia/inducido químicamente , Antagonistas del GABA/farmacología , Humanos , Técnicas In Vitro , Potenciales de la Membrana/efectos de los fármacos , Microelectrodos , Neuroglía/efectos de los fármacos , Neuroglía/fisiología , Neuronas/efectos de los fármacos , Neuronas/fisiología , Parasimpaticomiméticos/farmacología , Pilocarpina/farmacología , Ratas , Receptores de GABA-A/efectos de los fármacos , Receptores de N-Metil-D-Aspartato/efectos de los fármacosRESUMEN
Electrophysiological studies in humans and animal models have revealed an intrinsic epileptogenicity of cortical dysplasias which are a frequent cause of drug-resistant epilepsy. An imbalance of inhibition and excitation has been causative related. Receptor-binding studies in rodents demonstrated reduced binding to GABA and increased binding to glutamate receptors within cortical dysplasias and increments of AMPA- and kainate-receptor binding in its surround. Immunohistochemically a differential downregulation of GABA(A) receptor subunits could be demonstrated in widespread areas within and around dysplasias. As receptor binding critically depends on receptor subunit composition the observed changes in binding properties might be related to this. Here, we immunohistochemically analyzed the regional expression of four NMDA receptor subunits and two major AMPA- and kainate-receptor complexes in adult rats after neonatal freeze lesions. These lesions are characterized by a three- to four-layered cortex and a microsulcus which mimic human polymicrogyria. Using antibodies against NR1, NR2A, NR2B, NR2D, GluR2,3, and GluR5,6,7 receptor subunits we demonstrated a pronounced disturbance of cortical immunostaining pattern in the cortical malformation. These changes reflected the structural disorganization of the microgyrus with some distortion of the apical dendrites of paramicrogyral pyramidal cells, a decrease and disorganization of cells at the bottom of the microsulcus, and an inflection of apical dendrites toward the microsulcus. The neuronal staining pattern of large pyramidal cells in the neighborhood of the dysplasia did not differ for any subunit investigated. No remote or widespread changes of glutamate-receptor subunit distribution could be detected. The lack of gross and/or widespread alterations of glutamate-receptor subunit distribution in the surround of focal cortical dysplasia suggests the presence of other or additional mechanisms underlying the increased excitatory neurotransmitter binding and excitability in cortical malformations.
Asunto(s)
Corteza Cerebral/anomalías , Corteza Cerebral/metabolismo , Epilepsia/etiología , Epilepsia/metabolismo , Malformaciones del Sistema Nervioso/complicaciones , Malformaciones del Sistema Nervioso/metabolismo , Receptores de Glutamato/metabolismo , Potenciales de Acción/fisiología , Animales , Animales Recién Nacidos , Sitios de Unión/fisiología , Unión Competitiva/fisiología , Corteza Cerebral/fisiopatología , Epilepsia/fisiopatología , Potenciales Postsinápticos Excitadores/fisiología , Inmunohistoquímica , Malformaciones del Sistema Nervioso/fisiopatología , Inhibición Neural/fisiología , Subunidades de Proteína/metabolismo , Células Piramidales/metabolismo , Ratas , Ratas Wistar , Receptores AMPA/metabolismo , Receptores de Ácido Kaínico/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Transmisión Sináptica/fisiologíaRESUMEN
Recent evidence suggests that the expression of GABA(A) receptor subunits is determined by an early innate program which can be further modified by thalamic input and local factors. We analyzed the GABA(A) subunit distribution in experimentally induced subcortical heterotopia which are a subgroup of neuronal migration disorders. Heterotopias consist of clusters of neurons which have stopped migration early, before they have reached their final commitment and well before thalamic afferents have reached their targets. Immuno- histochemical analyses of five important GABA(A) receptor subunits revealed an expression pattern typical for upper cortical layers reflecting the original commitment of the heterotopic neurons. These results point towards detailed innate determinants of cell fate which even contain information on receptor subunit distribution and are not affected by ectopic positioning.
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Corteza Cerebral/metabolismo , Coristoma/metabolismo , Receptores de GABA-A/metabolismo , Animales , Animales Recién Nacidos , Corteza Cerebral/citología , Corteza Cerebral/crecimiento & desarrollo , Coristoma/inducido químicamente , Coristoma/patología , Agonistas de Aminoácidos Excitadores , Ácido Iboténico , Neuronas/citología , Neuronas/metabolismo , Ratas , Ratas Wistar , Receptores de GABA-A/biosíntesisRESUMEN
Cortical dysgenesis comprises a heterogenous group of genetic or acquired disturbances of cortical development which, due to progress in modern neuroimaging techniques, are increasingly recognized in association with a variety of clinical disorders. The spectrum of clinical manifestations, depending on type and extent of the alterations, includes severe mental retardation and epilepsy as well as neuropsychological deficits and psychiatric disorders. Up to now, the nomenclature of cortical malformations has been difficult and ambiguous. Recently, the understanding and terminology of these disorders has been facilitated by the proposal of a new classification scheme based on pathophysiological as well as pathogenetic mechanisms. This proposal has been elaborated by a group of experts and is not yet well-known in the German literature. Magnetic resonance imaging (MRI) allows diagnosis and classification in many cases of cortical dysgenesis during lifetime, thereby helping to identify prognostic and therapeutic options. Early diagnosis of cortical malformations is of particular importance in patients with drug-resistant epilepsy, as they can either be cured or benefit from epilepsy surgery. This review gives examples of the most relevant cortical malformations using the new classification scheme and summarizes their clinical as well as MRI characteristics. Besides routine MRI applications, some experimental techniques are discussed which may help to identify even subtle alterations.
Asunto(s)
Encefalopatías/clasificación , Encefalopatías/diagnóstico , Corteza Cerebral/anomalías , Imagen por Resonancia Magnética , Mutación , Encefalopatías/congénito , Encefalopatías/genética , Encefalopatías/patología , Corteza Cerebral/patología , Diagnóstico Diferencial , Epilepsia/genética , Epilepsia/patología , Pruebas Genéticas , Humanos , Discapacidad Intelectual/genética , Discapacidad Intelectual/patología , Fenotipo , Factores SexualesRESUMEN
Transient and permanent focal cerebral ischemia results in a series of typical pathophysiologic events. These consequences evolve in time and space and are not limited to the lesion itself, but they can be observed in perilesional (penumbra) and widespread ipsi- and sometimes contralateral remote areas (diaschisis). The extent of these areas is variable depending on factors such as the type of ischemia, the model, and the functional modality investigated. This review describes some typical alterations attributable to focal cerebral ischemia using the following classification scheme to separate different lesioned and perilesional areas: (1) The lesion core is the brain area with irreversible ischemic damage. (2) The penumbra is a brain region that suffers from ischemia, but in which the ischemic damage is potentially, or at least partially, reversible. (3) Remote brain areas are brain areas that are not directly affected by ischemia. With respect to the etiology, several broad categories of remote changes may be differentiated: (3a) remote changes caused by brain edema; (3b) remote changes caused by waves of spreading depression; (3c) remote changes in projection areas; and (3d) remote changes because of reactive plasticity and systemic effects. The various perilesional areas are not necessarily homogeneous; but a broad differentiation of separate topographic perilesional areas according to their functional state and sequelae allows segregation into several signaling cascades, and may help to understand the functional consequences and adaptive processes after focal brain ischemia.
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Isquemia Encefálica/patología , Encéfalo/patología , Encéfalo/fisiopatología , Isquemia Encefálica/diagnóstico , Isquemia Encefálica/fisiopatología , Humanos , Plasticidad Neuronal , Recuperación de la Función , Transmisión SinápticaRESUMEN
Early postnatal injections of ibotenate into the rat neopallium induce cortical dysplasias mimicking human polymicrogyria which often goes along with seizure disorders. Under in vitro conditions these experimentally induced dysplasias cause widespread hyperexcitability. The underlying mechanisms are as yet not fully understood. Electrophysiologically there is clear evidence of widespread alterations of the excitatory system. Intracellular recordings also showed some changes of the inhibitory system but have concentrated on recordings from focal areas close to the microgyrus. We investigated the integrity of functional inhibition using a paired-pulse paradigm to map the whole ipsilateral hemisphere. In rat cortical slices double-pulses were applied in layer VI/white matter and field potentials recorded in layer II/III. The ratio of the field potential amplitude did not show significant alterations in the dysplasias or their surround as compared with control and sham-injected animals. This result was obtained with two different locations of the dysplasias, excluding a mere areal specific effect. Our results show that despite prominent hyperexcitability in the surround of ibotenate-induced cortical dysplasias the inhibitory network appears to be functionally intact.
Asunto(s)
Corteza Cerebral/patología , Epilepsia/patología , Epilepsia/fisiopatología , Inhibición Neural/fisiología , Animales , Animales Recién Nacidos , Modelos Animales de Enfermedad , Electrofisiología , Agonistas de Aminoácidos Excitadores , Ácido Iboténico , Degeneración Nerviosa/inducido químicamente , Degeneración Nerviosa/patología , Degeneración Nerviosa/fisiopatología , Ratas , Ratas WistarRESUMEN
Focal cortical malformations comprise a heterogeneous group of disturbances of brain development, commonly associated with drug-resistant epilepsy and/or neuropsychological deficits. Electrophysiological studies on rodent models of cortical malformations demonstrated intrinsic hyperexcitability in the lesion and the structurally intact surround, indicating widespread imbalances of excitation and inhibition. Here, alterations in regional expression of GABA(A) receptor subunits were investigated immunohistochemically in adult rats with focal cortical malformations attributable to neonatal freeze-lesions. These lesions are morphologically characterized by a three- to four-layered cortex with microsulcus formation. Widespread regionally differential reduction of GABA(A) receptor subunits alpha1, alpha2, alpha3, alpha5, and gamma2 was observed. Within the cortical malformation, this downregulation was most prominent for subunits alpha5 and gamma2, whereas medial to the lesion, a significant and even stronger decrease of all subunits was detected. Lateral to the dysplastic cortex, the decrease was most prominent for subunit gamma2 and moderate for subunits alpha1, alpha2, and alpha5, whereas subunit alpha3 was not consistently altered. Interestingly, the downregulation of GABA(A) receptor subunits also involved the ipsilateral hippocampal formation, as well as restricted contralateral neocortical areas, indicating widespread disturbances in the neocortical and hippocampal network. The described pattern of downregulation of GABA(A) receptor subunits allows the conclusion that there is a considerable modulation of subunit composition. Because alterations in subunit composition critically influence the electrophysiological and pharmacological properties of GABA(A) receptors, these alterations might contribute to the widespread hyperexcitability and help to explain pharmacotherapeutic characteristics in epileptic patients.
Asunto(s)
Encéfalo/metabolismo , Regulación hacia Abajo , Receptores de GABA-A/genética , Animales , Animales Recién Nacidos , Encéfalo/patología , Corteza Cerebral/patología , Modelos Animales de Enfermedad , Epilepsias Parciales/genética , Epilepsias Parciales/metabolismo , Epilepsias Parciales/patología , Congelación , Necrosis , Especificidad de Órganos , Ratas , Ratas WistarRESUMEN
Cortical dysgenesia are a heterogenous group of genetic or acquired disturbances of cortical development which, due to the enormous progress in modern neuroimaging techniques, are increasingly recognized in association with a variety of clinical disorders. The spectrum of clinical manifestations, depending on type and extent of the alterations, includes severe mental retardation and epilepsy as well as neuropsychological deficits and some psychiatric disorders. Although pathogenesis and pathophysiology of cortical dysgenesis are still not fully understood, the recent discovery of responsible genes, growth factors, neurotransmitters, and exogenous factors sheds light on elementary mechanisms. The development of animal models mimicking different types of human cortical malformations helped to increase further the understanding of functional consequences of focal cortical dysgenesis. Several studies on these models reveal widespread alterations of cortical connectivity and excitability which are probably of crucial importance in different clinical disorders.
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Encefalopatías/etiología , Encéfalo/anomalías , Encéfalo/embriología , Anomalías Congénitas/etiología , Animales , Encefalopatías/genética , Encefalopatías/fisiopatología , Inhibición de Migración Celular , Movimiento Celular , Anomalías Congénitas/embriología , Anomalías Congénitas/genética , Anomalías Congénitas/fisiopatología , Modelos Animales de Enfermedad , Humanos , Mutación , NeuronasRESUMEN
Derivatives of the antiepileptic drug valproate (VPA, 2-propylpentanoic acid) have been synthesized and tested in order to improve the intracellular availability of VPA. The buccal ganglia of Helix pomatia were used as a test nervous system and antiepileptic efficacies were reconfirmed using rat cortex in vivo. Epileptiform activities consisted of typical paroxysmal depolarization shifts (PDS) which appeared in the identified neuron B3 with application of pentylenetetrazol. Epileptiform activities were found to be accelerated, unaffected or blocked. (i) The Amide-derivatives 2-propylpentanamide and N,N-dipropyl-2-propylpentanamide, and short chain ester derivatives 1-O-(2-propylpentanoyl)-2,3-propandiol, 2,2-di(hydroxymethyl)-1-O-(2-propylpentanoyl)-1,3-propanediol and 2,2-di(hydroxymethyl)-1,3-di-O-(2-propylpentanoyl)-1,3-propanediol accelerated epileptiform activities. Membrane potential often shifted to a permanent depolarization which corresponded to the PDS-inactivation level. (ii) The structural analogs 1-cycloheptene-1-carboxylic acid and cyclooctanecarboxylic acid accelerated epileptiform activities only slightly or were without effects. (iii) The small VPA-ester, 2-propylpentanoic acid ethyl ester, decreased the epileptiform activities in a way that is comparable to the effects of VPA well known from previous studies. It thus could be thought as a VPA-pro-drug. (iv) The mannitol-esters 1-O-(2-propylpentanoyl)-D-mannitol and 3,4;5,6-Di-O-isopropylidene-1-O-(2-propylpentanoyl)-D-mannitol blocked the PDS in a way which is different from the known effects of VPA. These substances are interpreted not to exert their effects after being metabolized to VPA and thus they are thought to be new antiepileptic substances.
Asunto(s)
Anticonvulsivantes/farmacología , Ácido Valproico/farmacología , Amidas/química , Amidas/farmacología , Animales , Anticonvulsivantes/química , Modelos Animales de Enfermedad , Ésteres/química , Ésteres/farmacología , Caracoles Helix , Relación Estructura-Actividad , Ácido Valproico/análogos & derivados , Ácido Valproico/químicaRESUMEN
The structure effect relationships of derivatives of the antiepileptically active ester of valproate (VPA) 3,4:5,6-Di-O-isopropylidene-1-O-(2-propylpentanoyl)-D-mannitol (1) have been studied using intracellular recording to record the membrane potential of single neurons (buccal ganglia, Helix pomatia). Epileptiform activity was induced by the epileptogenic drug pentylenetetrazol. The effects of several derivatives on epileptiform activity were compared with those of the relay compound 1. Most of the synthesized agents decreased the duration of paroxysmal depolarization shifts (PDS) and increased their repetition rate. It was considered that a decreased the duration of PDS is antiepileptic and an increased repetition rate is pro-epileptic. Compared with the effects of compound 1, the following relationships were found: (1) Derivatives containing glucitol or galactitol were of similar antiepileptic potency. (2) Introduction of pyranoses or furanoses rendered the substances inactive or even pro-epileptic. (3) VPA in position 1 and 6 at the sugar acted as an antiepileptic whereas in position 3 and 4 it proved to be ineffective. (4) Replacement of VPA by ethylhexanoyl reduced the antiepileptic potency slightly and pivaloyl strongly. (5) Replacement of isopropylidene bridges by penta-O-acetyl or cyclohexylidene residues led to largely inactive substances. (6) Compounds having isopropylidene bridges in position 2,4;3,5 proved to be antiepileptic whereas bridges especially in positions 2,3:4,5 slightly enhanced epileptic activities.
Asunto(s)
Anticonvulsivantes/farmacología , Ácido Valproico/farmacología , Animales , Anticonvulsivantes/química , Modelos Animales de Enfermedad , Electrofisiología , Ésteres/química , Ésteres/farmacología , Caracoles Helix , Relación Estructura-Actividad , Ácido Valproico/análogos & derivados , Ácido Valproico/químicaRESUMEN
Focal cortical lesions are associated with a functional downregulation of the GABAergic system in perilesional tissue lasting (at least) several weeks. The molecular mechanisms underlying this phenomenon are still poorly understood. Here we used RT-PCR to investigate whether mRNA-levels of two alpha-subunits of the GABAA-receptor (alpha1- and alpha2-subunits) change following ischemic cortical lesioning. The results show that 7 days after lesion induction mRNA-levels for both the alpha1- and alpha2-subunits are increased threefold in perilesional tissue ipsilateral, but not contralateral to the lesion. Taken together with the results of a previous immunohistochemical study in which a moderate decrease of the alpha1-subunit-protein and no change for the alpha2-subunit [T. Neumann-Haefelin, J.F. Staiger, C. Redecker, K. Zilles, J.M. Fritschy, H. Mohler, O.W. Witte, Immunohistochemical evidence for dysregulation of the GABAergic system ipsilateral to photochemically induced cortical infarcts in rats. Neuroscience (Oxford) 87 (4) (1998) 871-879] was observed, this is interpreted as a partial block of translation in the perilesional tissue surrounding cortical ischemic lesions.
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Isquemia Encefálica/metabolismo , ARN Mensajero/biosíntesis , Receptores de GABA-A/genética , Regulación hacia Arriba , Animales , Corteza Cerebral/irrigación sanguínea , Corteza Cerebral/metabolismo , Lateralidad Funcional , Masculino , Ratas , Ratas Wistar , Receptores de GABA-A/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Factores de TiempoRESUMEN
Malformations of cortical development are increasingly recognized in association with severe epileptic syndromes, neuropsychological disorders and mental retardation. Several clinical and experimental studies suggest that functional consequences of cortical dysplasias are not restricted to the area of the dysplastic lesion but also involve remote brain regions. In the present study cortical malformations were induced in newborn rats at day of birth by intracerebral injection of the glutamatergic agonist ibotenate. The resulting cytoarchitectonic lesion associates neuronal depopulation of deep cortical layers, ectopic neurons in superficial layers and sulcus formation, mimicking human polymicrogyria and migration disorders. Electrophysiological recordings of evoked field potentials in slice preparations of adult animals reveal hyperexcitability in widespread cortical regions surrounding the dysplasia. Low-intensity stimulation induced epileptiform activity consisting of long-lasting, multiphasic and N-methyl-D-aspartate-dependent field responses. They appeared with high variability as all-or-none events. These widespread changes in excitability were not observed in sham-operated animals with small superficial ectopias but intact deep cortical layers, indicating that focal loss of these layers induces extended alterations in cortical connectivity and imbalance of excitation and inhibition. Restricted zones of increased excitability were also found in the forelimb and hindlimb representation cortex in sham-operated and control animals, demonstrating that this activity has to be considered as an intrinsic property of specific cortical areas. Deoxyglucose autoradiography showed that the widespread hyperexcitability in ibotenate-injected animals was not accompanied by alterations in glucose metabolism, although in the area of structural abnormality a typical metabolic pattern was found, revealing an increased glucose uptake in layer I. Hypometabolism as described for many types of human dysplastic lesions was not observed. This difference between the experimental and clinical data may be due to the absence of behavioral seizures in this model. However, it can be hypothesized that in patients with developmental malformations, additional pathogenic factors contribute to the manifestation of seizure disorders.
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Corteza Cerebral/anomalías , Epilepsia/metabolismo , Glucosa/metabolismo , Animales , Animales Recién Nacidos , Autorradiografía , Modelos Animales de Enfermedad , Estimulación Eléctrica , Ácido Iboténico , Inmunohistoquímica , Técnicas In Vitro , Ratas , Ratas WistarRESUMEN
Deficits of GABAergic transmission have been reported to occur in tissue surrounding ischemic cortical lesions between a few days and several weeks after the insult. In the present experiments, we used immunohistochemistry with antibodies against parvalbumin and two major subunits of the GABA(A) receptor (alpha1, alpha2) to characterize the events that underlie these changes at different levels of circuit organization. Neocortical infarcts (2 mm diameter) consistently affecting medial parts of the primary somatosensory cortex were induced photochemically in adult male Wistar rats; animals were allowed to recover for one week before perfusion-fixation. When compared to controls the pattern of immunoreactivity had changed for the al subunit of the GABA(A) receptor seven days after the insult. Ipsilateral to the ischemic lesions, we found a decrease in staining intensity reaching up to 4 mm laterally, resulting in a partial or complete absence of the normal laminar staining pattern. No consistent changes were observed for the alpha2 subunit. Parvalbumin staining revealed pathological alterations in a rim of tissue surrounding the infarct, measuring up to 1 mm from the border of the infarcts. Parvalbumin-positive interneurons in this region showed signs of degeneration; both a reduction of the number of dendrites and, to a lesser extent and only immediately adjacent to the ischemic lesions, a reduction of the number of parvalbumin-positive neurons was readily apparent. The results provide evidence for both a differential regulation of two GABA(A) receptor subunits and degenerative changes of parvalbumin-containing interneurons ipsilateral to cortical infarcts. The relevance of these findings for mechanisms underlying long-term recovery, transient functional deficits and postinfarct seizures warrants further investigation.
Asunto(s)
Infarto Cerebral/metabolismo , Parvalbúminas/biosíntesis , Receptores de GABA-A/biosíntesis , Corteza Somatosensorial/metabolismo , Ácido gamma-Aminobutírico/fisiología , Animales , Isquemia Encefálica/etiología , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patología , Infarto Cerebral/etiología , Infarto Cerebral/patología , Dominancia Cerebral , Regulación de la Expresión Génica/efectos de la radiación , Interneuronas/química , Interneuronas/patología , Masculino , Degeneración Nerviosa , Proteínas del Tejido Nervioso/biosíntesis , Proteínas del Tejido Nervioso/genética , Plasticidad Neuronal , Neuronas/química , Neuronas/patología , Parvalbúminas/genética , Fotoquímica , Ratas , Ratas Wistar , Receptores de GABA-A/genética , Convulsiones/etiología , Convulsiones/fisiopatología , Corteza Somatosensorial/patologíaRESUMEN
Functional recovery after stroke is partly due to cortical reorganization on a structural as well as a functional level. Recent investigations have shown that the excitability of brain areas surrounding cortical ischemic lesions is increased, probably due to a down-regulation of gamma-aminobutyric acid-receptor activity. There is some evidence that these changes might increase the susceptibility of the lesioned brain for adaptive changes and recovery. Here, we investigated the propensity for the induction of long-term potentiation (LTP) in the surround of experimentally induced focal cortical infarcts in rat somatosensory cortex in vitro. By using standard paradigms, LTP induction was found to be facilitated ipsilaterally in slices of lesioned animals 1 week after lesion induction. In homotopic contralateral areas, LTP was not different from control values. As LTP is commonly associated with plasticity and learning, the results provide further evidence for the lesion-induced amplification of network plasticity, as it is required for the reshaping of cortical circuits by timely training procedures.