RESUMEN
ABSTRACT Evaluate the effect of the extract of Ginkgo biloba in the bone alkaline phosphatase, bone mineral density, in the mechanical properties of the tibia in rats with glucocorticoid-induced-osteoporosis. After osteoporosis induction, the rats were divided into five groups: Osteoporosis; EGb1 (28 mg/Kg); EGb2 (56 mg/Kg); alendronate (0.2 mg/animal) and control. The animals were treated during 20 and 30 days. The control group was compared with the osteoporosis's (Student's t-test), while the other were analyzed by ANOVA test followed by Tukey/Dunnett'T3 (p<0.05). In the osteoporosis group the bone alkaline phosphatase, bone mineral density, the bone stiffness, the maximum load and the resilience were reduced. The bone alkaline phosphatase values increased in the EGb1 and EGb2 groups (30 days). In addition, in the EGb2 and alendronate groups (20 and 30 days) the bone mineral density increased. The extract of Ginkgo biloba restored bone alkaline phosphatase and bone mineral density using dual-energy x-ray absorptiometry.
Asunto(s)
Animales , Femenino , Ratas , Osteoporosis/tratamiento farmacológico , Extractos Vegetales/farmacología , Densidad Ósea/efectos de los fármacos , Osteoblastos , Osteoporosis/inducido químicamente , Tibia , Ratas Wistar , Modelos Animales de Enfermedad , Fosfatasa Alcalina/metabolismo , GlucocorticoidesRESUMEN
Evaluate the effect of the extract of Ginkgo biloba in the bone alkaline phosphatase, bone mineral density, in the mechanical properties of the tibia in rats with glucocorticoid-induced-osteoporosis. After osteoporosis induction, the rats were divided into five groups: Osteoporosis; EGb1 (28 mg/Kg); EGb2 (56 mg/Kg); alendronate (0.2 mg/animal) and control. The animals were treated during 20 and 30 days. The control group was compared with the osteoporosis's (Student's t-test), while the other were analyzed by ANOVA test followed by Tukey/Dunnett'T3 (p<0.05). In the osteoporosis group the bone alkaline phosphatase, bone mineral density, the bone stiffness, the maximum load and the resilience were reduced. The bone alkaline phosphatase values increased in the EGb1 and EGb2 groups (30 days). In addition, in the EGb2 and alendronate groups (20 and 30 days) the bone mineral density increased. The extract of Ginkgo biloba restored bone alkaline phosphatase and bone mineral density using dual-energy x-ray absorptiometry.
Asunto(s)
Densidad Ósea/efectos de los fármacos , Osteoporosis/tratamiento farmacológico , Extractos Vegetales/farmacología , Fosfatasa Alcalina/metabolismo , Animales , Modelos Animales de Enfermedad , Femenino , Ginkgo biloba , Glucocorticoides , Osteoblastos , Osteoporosis/inducido químicamente , Osteoporosis/enzimología , Ratas , Ratas Wistar , TibiaRESUMEN
Ginkgo biloba is a plant used in the treatment of Alzheimer's disease, cerebrovascular insufficiency and peripheral vascular diseases that showed reproductive toxicity in vitro and in the mouse model. In this study, pregnant Wistar rats received 0, 3.5, 7 and 14mg/kgbodyweight/day of G. biloba, by gavage, from the 8th to 20th day of pregnancy. Rats were killed on the 21st day and the following parameters were evaluated: maternal body weight; food and water intake; maternal's liver, kidney and ovary weights; resorption index; post-implantation loss; mean of live fetuses; fetuses and placenta mean weight; fetuses' liver, kidney, lung and brain weights; fetuses' external malformations. No significant alteration was observed in maternal parameters of toxicity, but the treatment with 7 and 14mg/kg/day of G. biloba caused significant decrease in the fetuses mean weights. The results indicated that G. biloba was not toxic to mothers, although it caused fetal intra-uterine growth retardation.