Yield parameters and antioxidant compounds of tomato fruit: the role of plant defence inducers with or without Cucumber mosaic virus infection.

BACKGROUND: The production of fruit and vegetables rich in health-promoting components in an eco-friendly context represents the winning answer to the world population demand for food. In this study, the effects of different treatments on the yield and fruit chemical characteristics of tomato (Solanum lycopersicum L.) are reported. The treatments included three inducers of plant defence responses (chitosan, Trichoderma harzianum T-22 and Bacillus subtilis QST713) applied alone or before Cucumber mosaic virus infection. Fruit production and antioxidant compounds were investigated by ultrahigh-performance liquid chromatography (UHPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). RESULTS: Compared to control fruit harvested from untreated and healthy plants, treatment with QST713 increased the fruit number. Furthermore, plant treatments with T22, QST713 and chitosan alone enhanced fruit carotenoids (lutein and ß-carotene), ascorbic acid and phenolic acids (caffeoyl glucoside and p-coumaroyl glucoside). In parallel, compared to fruit harvested from only CMV-infected plants, treatments with T22, QST713 and chitosan before CMV enhanced fruit ascorbic acid and flavonoids (quercetin 3-O-xylosyl-rutinoside and rutin). CONCLUSION: Antioxidant compounds of tomato fruit can increase with the application of the plant defence inducers, thus protecting both the consumer and plant health. © 2019 Society of Chemical Industry.

Chitosan-elicited defense responses in Cucumber mosaic virus (CMV)-infected tomato plants.

The control of plant diseases by inducing plant resistance responses represents an interesting solution to avoid yield losses and protect the natural environment. Hence, the intertwined relationships between host, pathogen and inducer are increasingly subject of investigations. Here, we report the efficacy of chitosan-elicited defense responses in Solanum lycopersicum var. cerasiforme plants against Cucumber mosaic virus (CMV). Chitosan was applied via foliar spray before the CMV inoculation to verify its effectiveness as a preventive treatment against the viral infection. Virus accumulation, photosynthetic performance, as well as genes encoding for proteins affecting resistance responses and biosynthetic pathways, were investigated. It was observed a significant reduction of CMV accumulation in chitosan-treated plants that were successively infected with CMV, compared to only CMV-infected ones (up to 100%). Similarly, a positive effect of chitosan on gas exchange dynamics was revealed. The analysis of gene expression (CEVI-1, NPR1, PSY2 and PAL5) suggested the occurrence of chitosan-induced, systemic acquired resistance-related responses associated with a readjustment of the plant's oxidative status. In addition, the absence of deleterious symptoms in chitosan-treated successively CMV-infected plants, confirmed that chitosan can be used as a powerful control agent. Our data indicate that chitosan, when preventively applied, is able to elicit defense responses in tomato to control CMV infection. Such finding may be recommended to protect the tomato fruit yields as well as other crops.

Plant architecture, auxin homeostasis and phenol content in Arabidopsis thaliana grown in cadmium- and zinc-enriched media.

A screening strategy using micropropagation glass tubes with a gradient of distances between germinating seeds and a metal-contaminated medium was used for studying alterations in root architecture and morphology of Arabidopsis thaliana treated with cadmium (Cd) and zinc (Zn) at the concentration of 10-20µM and 100-200µM, respectively. Metal concentrations in plant shoots and roots were measured by quadrupole inductively coupled plasma mass spectrometry. After 21days from germination, all plants in the tubes were scanned at high resolution and the root systems analyzed. The localization of indole-3-acetic acid (IAA) in the primary root and lateral root apices was monitored using DR5:GUS, LAX3:GUS and AUX1:GUS Arabidopsis transgenic lines. Total phenol content in leaves was measured spectrophotometrically. Shoot and root dry weight and leaf area did not change in Zn-exposed plants and significantly decreased in Cd-exposed plants, compared to control plants. Cadmium induced a reduction of root length, of mean number of roots and of total root surface. Both Cd- and Zn-exposed plants showed a reduced specific root length. This morphological behavior, together with an observed increase in root diameter in metal-exposed plants could be interpreted as compensatory growth, and the observed thicker roots could act as a barrier to protect root from the metals. In comparison with the apical localization of the IAA signal in the control plants, Zn generally reinforced the intensity of IAA signal, without affecting its localization. In Cd-exposed plants, IAA localization remained apical but weaker compared to control plants. Total phenols decreased in plants exposed to Zn and Cd. Therefore, we propose that the remodelling of the root architecture and the production of some secondary metabolites, such as IAA and phenols could be two responses of plants subjected to metal stress. This knowledge can open the way to future phytoremediation strategies of contaminated sites.