RESUMEN
BACKGROUND AND OBJECTIVE: Human gingival epithelial cells (HGECs) are continually exposed to oral bacteria and to other harmful agents. Their responses to stimuli are critical in maintaining periodontal homeostasis. The aim of this study was to investigate the modulating effect of cigarette smoke extract (CSE) on the innate immune responses of HGECs. MATERIAL AND METHODS: Toll-like receptor (TLR) expression of HGECs was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). The effect of CSE or nicotine on the expression of the antimicrobial peptide human beta-defensin-2 (hBD-2) and the pro-inflammatory cytokine interleukin (IL)-8 in stimulated HGEC cultures was evaluated by RT-PCR and enzyme-linked immunosorbent assay. RESULTS: The HGECs expressed mRNA of TLRs 1, 2, 3, 5, 6, 9, 10, and minimally of TLR4, but not of TLRs 7 or 8. Stimulation of HGECs with highly purified TLR2, 3 or 5 ligands led to expression of hBD-2 and of IL-8. Enhancement of hBD-2 and IL-8 was observed in HGECs after combined stimulation with Porphyromonas gingivalis lipopolysaccharide (TLR2 ligand) and tumour necrosis factor-alpha, compared with stimulation using either agent alone. After CSE exposure, hBD-2 expression was markedly reduced in stimulated HGEC cultures, whereas IL-8 expression was markedly increased. These effects were also observed, but were markedly attenuated, upon nicotine treatment. CONCLUSION: Human gingival epithelial cells play a critical role in orchestrating the innate immune responses of periodontal tissue via TLR signalling. Our results represent the first demonstration that CSE can modulate HGEC function by suppressing hBD-2 and enhancing IL-8 production, and this may be, in part, a possible mechanism which promotes periodontal disease.
Asunto(s)
Antiinfecciosos/análisis , Encía/inmunología , Mediadores de Inflamación/análisis , Interleucina-8/análisis , Nicotiana/química , Humo/análisis , beta-Defensinas/análisis , Células Cultivadas , Células Epiteliales/citología , Células Epiteliales/inmunología , Encía/citología , Humanos , Inmunidad Innata/inmunología , Ligandos , Lipopolisacáridos/farmacología , Nicotina/farmacología , Agonistas Nicotínicos/farmacología , Porphyromonas gingivalis , Receptor Toll-Like 1/análisis , Receptor Toll-Like 10/análisis , Receptor Toll-Like 2/análisis , Receptor Toll-Like 2/efectos de los fármacos , Receptor Toll-Like 3/análisis , Receptor Toll-Like 3/efectos de los fármacos , Receptor Toll-Like 4/análisis , Receptor Toll-Like 4/efectos de los fármacos , Receptor Toll-Like 5/análisis , Receptor Toll-Like 6/análisis , Receptor Toll-Like 9/análisis , Receptor Toll-Like 9/efectos de los fármacos , Receptores Toll-Like/análisis , Factor de Necrosis Tumoral alfa/farmacología , beta-Defensinas/efectos de los fármacosRESUMEN
Interleukin (IL)-17 is present in inflammatory periodontal lesions, thus suggesting a role in mediating inflammation. We tested the hypothesis that IL-17, especially when combined with interferon (IFN)-gamma, may modulate the responses of human gingival fibroblasts (HGFs). IL-17 induced IL-8 and minimal intercellular adhesion molecule (ICAM)-1 expression. It had no effect on expression of HLA-DR, CD40, or the immune-suppressive enzyme indoleamine 2,3-dioxygenase (IDO). The effects of IL-17 on HGFs were compared with those of IFN-gamma. Unlike IL-17, IFN-gamma augmented the expression of HLA-DR, ICAM-1, and IDO, but not IL-8. Thus, IL-17 and IFN-gamma induce different HGF responses when administered separately. Interestingly, when IL-17 and IFN-gamma were combined, marked enhancement of ICAM-1, IL-8, and IDO expression by HGFs was observed. These findings suggest that IL-17, especially when combined with IFN-gamma, could play an important role in immune modulation through stimulation of HGFs in periodontal disease.
