RESUMEN
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA suquence from a database provides enough information to make them. (AU)
Asunto(s)
Ratones , Animales , Formación de Anticuerpos , Biblioteca de Péptidos , Fosfoproteínas Fosfatasas , Oligonucleótidos , Ratones Endogámicos C57BL , Análisis de Secuencia de ADN , Conejos , Western Blotting , Inmunohistoquímica , Oligonucleótidos/biosíntesis , Indicadores y Reactivos , Reacción en Cadena de la PolimerasaRESUMEN
Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA suquence from a database provides enough information to make them.