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1.
Sensors (Basel) ; 24(12)2024 Jun 10.
Artículo en Inglés | MEDLINE | ID: mdl-38931556

RESUMEN

This paper reports a rapid and sensitive sensor for the detection and quantification of the COVID-19 N-protein (N-PROT) via an electrochemical mechanism. Single-frequency electrochemical impedance spectroscopy was used as a transduction method for real-time measurement of the N-PROT in an immunosensor system based on gold-conjugate-modified carbon screen-printed electrodes (Cov-Ag-SPE). The system presents high selectivity attained through an optimal stimulation signal composed of a 0.0 V DC potential and 10 mV RMS-1 AC signal at 100 Hz over 300 s. The Cov-Ag-SPE showed a log response toward N-PROT detection at concentrations from 1.0 ng mL-1 to 10.0 µg mL-1, with a 0.977 correlation coefficient for the phase (θ) variation. An ML-based approach could be created using some aspects observed from the positive and negative samples; hence, it was possible to classify 252 samples, reaching 83.0, 96.2 and 91.3% sensitivity, specificity, and accuracy, respectively, with confidence intervals (CI) ranging from 73.0 to 100.0%. Because impedance spectroscopy measurements can be performed with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing, even in places with limited resources, as an alternative to the common diagnostics methods.


Asunto(s)
Técnicas Biosensibles , COVID-19 , Espectroscopía Dieléctrica , Oro , SARS-CoV-2 , COVID-19/diagnóstico , COVID-19/virología , Técnicas Biosensibles/métodos , Técnicas Biosensibles/instrumentación , Humanos , SARS-CoV-2/aislamiento & purificación , SARS-CoV-2/inmunología , Espectroscopía Dieléctrica/instrumentación , Espectroscopía Dieléctrica/métodos , Oro/química , Electrodos , Técnicas Electroquímicas/métodos , Técnicas Electroquímicas/instrumentación , Inmunoensayo/métodos , Inmunoensayo/instrumentación , Proteínas de la Nucleocápside de Coronavirus/inmunología , Proteínas de la Nucleocápside de Coronavirus/análisis , Carbono/química , Fosfoproteínas/análisis
2.
PLoS One ; 19(6): e0304205, 2024.
Artículo en Inglés | MEDLINE | ID: mdl-38857245

RESUMEN

Neuromuscular electrical stimulation (NMES) can improve physical function in different populations. NMES-related outcomes may be influenced by muscle length (i.e., joint angle), a modulator of the force generation capacity of muscle fibers. Nevertheless, to date, there is no comprehensive synthesis of the available scientific evidence regarding the optimal joint angle for maximizing the effectiveness of NMES. We performed a systematic review to investigate the effect of muscle length on NMES-induced torque, discomfort, contraction fatigue, and strength training adaptations in healthy and clinical adult populations (PROSPERO: CRD42022332965). We conducted searches across seven electronic databases: PUBMED, Web of Science, EMBASE, PEDro, BIREME, SCIELO, and Cochrane, over the period from June 2022 to October 2023, without restricting the publication year. We included cross-sectional and longitudinal studies that used NMES as an intervention or assessment tool for comparing muscle lengths in adult populations. We excluded studies on vocalization, respiratory, or pelvic floor muscles. Data extraction was performed via a standardized form to gather information on participants, interventions, and outcomes. Risk of bias was assessed using the Revised Cochrane risk-of-bias tool for cross-over trials and the Physiotherapy Evidence Database scale. Out of the 1185 articles retrieved through our search strategy, we included 36 studies in our analysis, that included 448 healthy young participants (age: 19-40 years) in order to investigate maximum evoked torque (n = 268), contraction fatigability (n = 87), discomfort (n = 82), and muscle strengthening (n = 22), as well as six participants with spinal cord injuries, and 15 healthy older participants. Meta-analyses were possible for comparing maximal evoked torque according to quadriceps muscle length through knee joint angle. At optimal muscle length 50° - 70° of knee flexion, where 0° is full extension), there was greater evoked torque during nerve stimulation compared to very short (0 - 30°) (p<0.001, CI 95%: -2.03, -1.15 for muscle belly stimulation, and -3.54, -1.16 for femoral nerve stimulation), short (31° - 49°) (p = 0.007, CI 95%: -1.58, -0.25), and long (71° - 90°) (p<0.001, CI 95%: 0.29, 1.02) muscle lengths. At long muscle lengths, NMES evoked greater torque than very short (p<0.001, CI 95%: -2.50, -0.67) and short (p = 0.04, CI 95%: -2.22, -0.06) lengths. The shortest quadriceps length generated the highest perceived discomfort for a given current amplitude. The amount of contraction fatigability was greater when muscle length allowed greater torque generation in the pre-fatigue condition. Strength gains were greater for a protocol at the optimal muscle length than for short muscle length. The quality of evidence was very high for most comparisons for evoked torque. However, further studies are necessary to achieve certainty for the other outcomes. Optimal muscle length should be considered the primary choice during NMES interventions, as it promotes higher levels of force production and may facilitate the preservation/gain in muscle force and mass, with reduced discomfort. However, a longer than optimal muscle length may also be used, due to possible muscle lengthening at high evoked tension. Thorough understanding of these physiological principles is imperative for the appropriate prescription of NMES for healthy and clinical populations.


Asunto(s)
Contracción Muscular , Fatiga Muscular , Músculo Esquelético , Torque , Humanos , Adulto , Fatiga Muscular/fisiología , Músculo Esquelético/fisiología , Contracción Muscular/fisiología , Estimulación Eléctrica/métodos , Fuerza Muscular/fisiología , Adaptación Fisiológica/fisiología , Terapia por Estimulación Eléctrica/métodos
3.
J Environ Manage ; 360: 121102, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38759561

RESUMEN

Marine protected areas (MPAs) are zones geographically delimited under pre-defined management goals, seeking to reduce anthropogenic threats to biodiversity. Despite this, in recent years reports of MPAs affected by chemical contamination has grown. Therefore, this study addresses this critical issue assessing legacy and current chemical contamination in filter-feeder bivalves obtained in very restrictive no-take MPAs from Brazil. The detected pollutants encompass polycyclic aromatic hydrocarbons (PAHs), linear alkylbenzenes (LABs), and persistent organic pollutants (POPs) like dichlorodiphenyltrichloroethane (DDTs) and polychlorinated biphenyls (PCBs). Despite protective measures, bivalves from nine MPAs exhibited high LABs (13.2-1139.0 ng g-1) and DDTs levels (0.1-62.3 ng g-1). PAHs were present in low concentrations (3.1-29.03 ng g-1), as PCBs (0.7-6.4 ng g-1), hexachlorobenzene (0.1-0.2 ng g-1), and Mirex (0.1-0.3 ng g-1). Regardless of the sentinel species, MPAs and management categories, similar accumulation patterns were observed for LABs, DDTs, PAHs, and PCBs. Based on the limits proposed by Oslo Paris Commission, the measured levels of PAHs, PCBs and were below the environmental assessment criteria. Such findings indicate the no biological effects are expected to occur. However, they are higher considering background conditions typically measured in remote or pristine areas and potential simultaneous exposure. Such findings indicate an influence of anthropogenic sources, emphasizing the urgency for monitoring programs guiding strategic management efforts to safeguard these areas.


Asunto(s)
Bivalvos , Monitoreo del Ambiente , Bifenilos Policlorados , Hidrocarburos Policíclicos Aromáticos , Contaminantes Químicos del Agua , Animales , Brasil , Hidrocarburos Policíclicos Aromáticos/análisis , Bifenilos Policlorados/análisis , Contaminantes Químicos del Agua/análisis , Contaminantes Orgánicos Persistentes
4.
J Med Virol ; 96(3): e29551, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38506236

RESUMEN

Respiratory Syncytial Virus (RSV) is an important cause of respiratory infection in humans. Severe cases are common in children ≤2 years old, immunocompromised individuals, and the elderly. In 2020, RSV infection reduced in Rio Grande do Sul (RS), southern Brazil; however, in 2021 resurgence of RSV was observed. This study analyzed epidemiological and genetic features of RSV infection cases reported in 2021 in RS. Nasopharyngeal samples collected from individuals with respiratory infection negative for SARS-CoV-2, Influenza A and B viruses were assessed for the presence of RSV by real time RT-qPCR. RSV-A and RSV-B genomic sequencing and phylogenetic reconstructions were performed for genotyping and clade characterization. Among 21,035 respiratory samples analyzed, 2,947 were positive for RSV, 947 of which were hospitalized patients. Positive cases were detected year-round, with the highest number in June-July (winter). Children <1 year comprised 56.28% (n = 533) of the hospitalized patients infected with RSV, whereas 14.46% (n = 137) were individuals >60 years. Of a total of 361 deaths, 14.68% (n = 53) were RSV positive, mostly patients >60 years old (73.58%, n = 39). Chronic kidney disease, cardiopathy, Down syndrome and neurological diseases were associated with RSV infection. RSV-A was identified in 58.5% (n = 117/200) of the patients, and RSV-B in 41.5% (n = 83/200). Of 95 RSV genomes recovered from SARI cases, 66 were RSV-A GA.2.3.5 genotype, while 29 were RSV-B GB.5.0.5a genotype. This study provides epidemiological and molecular data on RSV cases in RS during the COVID-19 pandemic and highlights that investigation of different respiratory viruses is essential for decision-making and disease prevention and control measures.


Asunto(s)
COVID-19 , Gripe Humana , Infecciones por Virus Sincitial Respiratorio , Virus Sincitial Respiratorio Humano , Infecciones del Sistema Respiratorio , Niño , Humanos , Lactante , Anciano , Preescolar , Persona de Mediana Edad , Virus Sincitial Respiratorio Humano/genética , Infecciones por Virus Sincitial Respiratorio/epidemiología , Filogenia , Brasil/epidemiología , Pandemias , COVID-19/epidemiología , SARS-CoV-2/genética , Gripe Humana/epidemiología
6.
Mar Pollut Bull ; 201: 116203, 2024 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-38422825

RESUMEN

Microplastic pollution is becoming a continuously growing environmental concern, while bivalve mollusks are particularly vulnerable due to their sessile habits and feeding through water filtration processes. Microplastic incidence in soft tissues of the clam Amarilladesma mactroides was assessed along unconsolidated substrates distributed in extensive coastal regions of southern Brazil. Influence of urbanization levels, distance to rivers and local hydrodynamics on microplastic accumulation by the clam was tested. The average concentration of microplastics was high (3.09 ± 2.11 particles.g-1), considering 16 sampled sites. Particles were mainly composed by polyamide, polyethylene and polyethylene terephthalate, while were mainly smaller, fibrous and colorless. High urbanization and closer proximity to rivers insured higher contamination, which is a trend observed globally. No influence of coastal hydrodynamics was seen. Considering obtained findings, A. mactroides presents good potential to be used as a valuable tool to assess microplastic contamination in unconsolidated substrates of beach areas.


Asunto(s)
Bivalvos , Contaminantes Químicos del Agua , Animales , Microplásticos , Plásticos , Brasil , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente
7.
Epigenetics ; 19(1): 2305082, 2024 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-38245873

RESUMEN

Physical activity is a first-line treatment for polycystic ovary syndrome (PCOS). Resistance or aerobic exercise improves metabolic complications, reproductive outcomes, and quality of life in PCOS. DNA methylation reprogramming during exercise may be the major modifier behind these changes. We sought to evaluate genome-wide DNA methylation changes after supervised resistance and aerobic exercise in women with PCOS. Exercises were performed in 56 women with PCOS (resistance, n = 30; aerobic, n = 26), for 16 weeks (wks), three times per week, in 50-minute to one-hour sessions. Anthropometric indices and hormonal and metabolic parameters were measured before and after training. Genome-wide leukocyte DNA methylation was analysed by Infinium Human MethylationEPIC 850K BeadChip microarrays (Illumina). Both resistance and aerobic exercise improved anthropometric indices, metabolic dysfunction, and hyperandrogenism in PCOS after the training programme, but no differences were observed between the two exercises. Resistance and aerobic exercise increased genome-wide DNA methylation, although resistance changed every category in the CpG island context (islands, shores, shelve, and open sea), whereas aerobic exercise altered CpG shores and the open sea. Using a stringent FDR (>40), 6 significantly differentially methylated regions (DMRs) were observed in the resistance exercise cohort and 14 DRMs in the aerobic cohort, all of which were hypermethylated. The increase in genome-wide DNA methylation may be related to the metabolic and hormonal changes observed in PCOS after resistance and aerobic exercise. Since the mammalian genome is hypermethylated globally to prevent genomic instability and ageing, resistance and aerobic exercise may promote health and longevity through environmentally induced epigenetic changes.


Asunto(s)
Metilación de ADN , Síndrome del Ovario Poliquístico , Animales , Femenino , Humanos , Síndrome del Ovario Poliquístico/genética , Síndrome del Ovario Poliquístico/terapia , Promoción de la Salud , Calidad de Vida , ADN , Mamíferos
8.
Mar Pollut Bull ; 200: 116045, 2024 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-38266479

RESUMEN

The contamination of mangroves by anthropogenic litter has increased in recent decades. Notably, Brazil occupies a prominent status within Latin America, boasting the second-largest mangrove areas globally. In Santos-São Vicente Estuarine System (SESS), mangroves coexist with a preeminent port complex and substantial urbanization rates. Nevertheless, the anthropogenic litter occurrence and distribution in this ecosystem remains unknown. This study aimed to comprehensively assess anthropogenic litter across 13 strategically positioned sites in the SESS. The total litter density (Mean ± SD) was 22.84 ± 36.47 (0.00-142.00) items·m-2, putting the SESS among the top four most contaminated mangrove ecosystems worldwide. Residential zones accumulated more litter than uninhabited areas and significant correlation was seen with human modification index. Plastic was the prevalent material (70.4 %), measuring mostly between 2.5 and 30 cm (41.1 %). It is imperative that local authorities adopt comprehensive strategies to mitigate contamination, while also curtailing the litter inputs to the SSES mangrove ecosystem.


Asunto(s)
Ecosistema , Monitoreo del Ambiente , Humanos , Brasil , Urbanización , Plásticos
9.
Int J Mol Sci ; 24(21)2023 Nov 01.
Artículo en Inglés | MEDLINE | ID: mdl-37958867

RESUMEN

The skin is essential to the integrity of the organism. The disruption of this organ promotes a wound, and the organism starts the healing to reconstruct the skin. Copaifera langsdorffii is a tree used in folk medicine to treat skin affections, with antioxidant and anti-inflammatory properties. In our study, the oleoresin of the plant was associated with nanostructured lipid carriers, aiming to evaluate the healing potential of this formulation and compare the treatment with reference drugs used in wound healing. Male Wistar rats were used to perform the excision wound model, with the macroscopic analysis of wound retraction. Skin samples were used in histological, immunohistochemical, and biochemical analyses. The results showed the wound retraction in the oleoresin-treated group, mediated by α-smooth muscle actin (α-SMA). Biochemical assays revealed the anti-inflammatory mechanism of the oleoresin-treated group, increasing interleukin-10 (IL-10) concentration and decreasing pro-inflammatory cytokines. Histopathological and immunohistochemical results showed the improvement of re-epithelialization and tissue remodeling in the Copaifera langsdorffii group, with an increase in laminin-γ2, a decrease in desmoglein-3 and an increase in collagen remodeling. These findings indicate the wound healing potential of nanostructured lipid carriers associated with Copaifera langsdorffii oleoresin in skin wounds, which can be helpful as a future alternative treatment for skin wounds.


Asunto(s)
Fabaceae , Repitelización , Ratas , Animales , Ratas Wistar , Piel/patología , Antiinflamatorios/farmacología , Antiinflamatorios/uso terapéutico , Fabaceae/química , Lípidos
10.
Nat Prod Res ; : 1-5, 2023 Nov 02.
Artículo en Inglés | MEDLINE | ID: mdl-37915254

RESUMEN

Propolis is a natural product widely used in folk medicine. Among its various applications, its antiparasitic properties stand out. Due to its great biodiversity, Brazil is a major producer of several types of propolis. This study proposes to evaluate the leishmanicidal properties of the hydroalcoholic extract of propolis collected in the southern region of Brazil (Brown propolis - HEBP) and its main isolated compounds: abietic acid (1), 13-epi-cupressic acid (2), 13-epi-torulosol (3), dehydroabietic acid (4), cis-communic acid (5) and ent-agatic acid (6). In general, the diterpenes did not show activity against the promastigotes of Leishmania (Leishmania) amazonensis at the evaluated concentrations. However, the HEBP was very active with an inhibition concentration of 50% at 8.32 µg/mL. Moreover, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) assays showed morphological and structural alterations in promastigote forms of L. (L.) amazonensis when incubated with HEBP.

11.
Sci Rep ; 13(1): 14397, 2023 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-37658115

RESUMEN

With the SARS-CoV-2 pandemic and the need for affordable and rapid mass testing, colorimetric isothermal amplification reactions such as Loop-Mediated Isothermal Amplification (LAMP) are quickly rising in importance. The technique generates data that is similar to quantitative Polymerase Chain Reaction (qPCR), but instead of an endpoint color visualization, it is possible to construct a signal over a time curve. As the number of works using time-course analysis of isothermal reactions increases, there is a need to analyze data and standardize their related treatments quantitatively. Here, we take a step forward toward this goal by evaluating different available data treatments (curve models) for amplification curves, which allows for a cycle threshold-like parameter extraction. In this study, we uncover evidence of a double sigmoid equation as the most adequate model to describe amplification data from our remote diagnostics system and discuss possibilities for similar setups. We also demonstrate the use of multimodal Gompertz regression models. Thus, this work provides advances toward standardized and unbiased data reporting of Reverse Transcription (RT) LAMP reactions, which may facilitate and quicken assay interpretation, potentially enabling the application of machine learning techniques for further optimization and classification.


Asunto(s)
COVID-19 , Humanos , COVID-19/diagnóstico , Colorimetría , SARS-CoV-2/genética , Bioensayo
12.
BMC Res Notes ; 16(1): 149, 2023 Jul 17.
Artículo en Inglés | MEDLINE | ID: mdl-37461048

RESUMEN

OBJECTIVES: Surveillance of infant and fetal deaths is of paramount importance in thinking about government strategies to reduce these rates, provide greater visibility of these mortality figures in the country, enable the adoption of prevention measures, as well as contribute to a better record of deaths. DATA DESCRIPTION: The dataset comprises fetal, neonatal, early neonatal, late neonatal, and perinatal Mortality Rates of Brazilian municipalities with their respective information, between 2010 to 2020, aggregated by epidemiological week.


Asunto(s)
Muerte Fetal , Mortalidad Infantil , Lactante , Recién Nacido , Embarazo , Femenino , Humanos , Brasil/epidemiología , Mortalidad Perinatal , Atención Prenatal
13.
BMC Res Notes ; 16(1): 151, 2023 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-37475018

RESUMEN

OBJECTIVES: The National Registry of Healthcare Facilities is a system with the registry of every healthcare facility in Brazil with information on the capacity building and healthcare workforce regarding its public or private nature. Despite being publicly available, it can only be accessed in separated disjoint tables, with different primary units of analysis. The objective is to offer an interoperable dataset containing monthly data from 2005 to 2021 with information on healthcare facilities, including their physical and human resources, services and teams, enriched with municipal information. DATA DESCRIPTION: Database with historical data and geographic information for each health facility in Brazil. It is composed by 5 distinct tables, organized according to combinations of time, space, and types of resources, services and teams. This database opens up a range of possibilities for research topics, from case studies in a single health facility and period, analysis of a group of health facilities with characteristics of interest, to a broader study using the entire dataset and aggregated data by municipality. Furthermore, the fact that there is a row for each health facility/month/year facilitates the integration with other datasets from the Brazilian healthcare system. In addition to being a potential object of study in the health area, the dataset is also convenient in data science, especially for studies focused on time series.


Asunto(s)
Conjuntos de Datos como Asunto , Instituciones de Salud , Brasil , Sistema de Registros
14.
Environ Sci Pollut Res Int ; 30(27): 71396-71408, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37199842

RESUMEN

Peru suffers from poor solid waste and coastal management, as well as evidenced plastic pollution in various forms. However, studies in Peru focusing on small plastic debris (i.e., meso- and microplastics) are still limited and inconclusive. Thus, the present study investigated the abundance, characteristics, seasonality, and distribution of small plastic debris along the coast of Peru. The abundance of small plastic debris is predominantly driven by specific locations, where a source of contamination is present, rather than presenting seasonal patterns. Meso- and microplastics were strongly correlated in both seasons (summer and winter), suggesting meso-plastic constantly breaking down as microplastic sources. Additionally, heavy metals (e.g., Cu, Pb) were found in low concentrations (mean concentrations < 0.4%) on the surface of some mesoplastics. Here, we provided a baseline on the multiple factors involving small plastic debris on the Peruvian coast and preliminarily identify associated contaminants.


Asunto(s)
Microplásticos , Contaminantes Químicos del Agua , Plásticos , Perú , Residuos/análisis , Monitoreo del Ambiente , Residuos Sólidos , Contaminantes Químicos del Agua/análisis
15.
Arch Endocrinol Metab ; 67(5): e000627, 2023 May 25.
Artículo en Inglés | MEDLINE | ID: mdl-37249458

RESUMEN

Objective: This study aimed to determine the differences in body fat distribution and central obesity indicators using dual-energy X-ray absorptiometry (DXA), adiposity indices, and anthropometric indices between women with and without polycystic ovary syndrome (PCOS). Materials and methods: Clinical and laboratory examination history, including transvaginal ultrasound, fasting blood samples, anthropometric measurements, and DXA scans were conducted in 179 women with PCOS (PCOS group) and 100 without PCOS (non-PCOS group). The volunteers were grouped by body mass index (BMI): normal (18-24.9 kg/m2), overweight (25-29.9 kg/m2), or obese (>30 kg/m2). The visceral adiposity index (VAI) and lipid accumulation product (LAP) were calculated, regions of interest (ROIs) were determined, and the fat mass index (FMI) was calculated using DXA. Results: VAI, LAP, ROIs, FMI, and adiposity indices by DXA were higher in women with PCOS and normal BMI. In both PCOS and non-PCOS groups, the ROIs progressively increased from normal BMI to overweight and obese, and from overweight to obese. Obese women with PCOS showed high trunk fat mass. However, obesity was not able to modify these trunk/periphery fat ratios in PCOS from overweight to higher BMI. These variables were associated with the incidence of PCOS. Conclusion: In women with PCOS and normal BMI, both DXA and the adiposity indices, VAI and LAP, are more sensitive methods to evaluate total body fat and fat accumulation in the central abdominal region. It was also observed that as BMI increased, the differences in measurements between women with and without PCOS decreased.


Asunto(s)
Resistencia a la Insulina , Síndrome del Ovario Poliquístico , Femenino , Humanos , Adiposidad , Índice de Masa Corporal , Absorciometría de Fotón , Sobrepeso , Obesidad/complicaciones , Obesidad Abdominal/diagnóstico por imagen , Obesidad Abdominal/complicaciones
16.
Mar Pollut Bull ; 191: 114941, 2023 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-37080019

RESUMEN

Marine litter is a complex environmental issue threatening the well-being of multiple organisms. In the present study, we present an overlooked pathway by which marine litter interaction with certain ovigerous skates (Family: Rajidae) communities could compromise their survival. We propose that skates from the genus Sympterygia deposit their egg capsules on marine litter substrates by accident, which are then washed ashore still unhatched. We conducted 10 monitoring surveys on three beaches of La Libertad Region, on the north coast of Peru, looking for marine litter conglomerates to determine the presence of egg capsules. We registered a total of 75 marine litter conglomerates, containing 1595 egg capsules, out of which only 15.9 % were presumably hatched, and 15.8 % were still fresh. Fishing materials were identified as the main item in marine litter conglomerates. We conclude that this behavior could contribute to the decline of Sympterygia communities, although further research is needed.


Asunto(s)
Monitoreo del Ambiente , Residuos , Residuos/análisis , Cápsulas , Plásticos , Caza , Playas
17.
Psicopedagogia ; 40(121): 28-37, jan.-abr. 2023.
Artículo en Portugués | LILACS-Express | LILACS, Index Psicología - Revistas | ID: biblio-1448999

RESUMEN

O presente estudo avaliou a associação da saúde mental com fatores do ambiente de trabalho de professores brasileiros. Para tanto, foram aplicados um questionário semiestruturado com questões socioeconômicas e o Hospital Anxiety and Depression Scale para 499 professores, recrutados por meio do Facebook e Instagram. Desses, 391 eram do gênero feminino e 108 do masculino. Verificou-se que 32,7% apresentaram escore indicativo de improvável quadro de ansiedade; 26,8% possível e 40,5% provável. Por outro lado, 45,1% exibiram escore de improvável depressão; 31,5% possível e 23,4% provável. Foi identificado que ser do gênero feminino interferiu negativamente nos escores de ansiedade e depressão (p<0,001); assim como a inadequação e insatisfação com a renda (p<0,001); a classificação como péssimo ou regular ambiente de trabalho (p<0,001); o espaço físico inadequado (p<0,001); ter sofrido violência provocada pelos estudantes ou seus respectivos pais (p<0,001). Além disso, trabalhar por um tempo ≥40 horas (p=0,030) foi indicativo de maior ansiedade. Muitos professores brasileiros sofrem com quadros de ansiedade e depressão e fatores controláveis interferem diretamente. Sugere-se que o poder público e os proprietários de escolas privadas intervenham nesses contextos para evitar o colapso da saúde desses profissionais.


The present study evaluated the association between mental health and work environment factors among Brazilian teachers. In order to achieve that, a semi-structured questionnaire with socioeconomic questions and the Hospital Anxiety and Depression Scale were applied to 499 teachers, recruited through Facebook and Instagram. Among these, 391 were female and 108 were male. It was found that 32.7% had an improbable anxiety score; 26.8% possible and 40.5% probable. On the other hand, 45.1% had an improbable depression score; 31.5% possible and 23.4% probable. It was identified that being female had a negative effect on anxiety and depression scores (p<0.001); as well as inadequacy and dissatisfaction with income (p<0.001); work environment classified as poor or regular (p<0.001); inadequate physical space (p<0.001); having suffered violence caused by students or their respective parents (p<0.001). Furthermore, working for 40 hours or else (p=0.030) was indicative of greater anxiety. Many Brazilian teachers suffer from anxiety and depression and controllable factors interfere directly. It is suggested that public authorities and private school owners intervene in these contexts to prevent the health of these professionals from collapsing.

18.
Sci Total Environ ; 874: 162468, 2023 May 20.
Artículo en Inglés | MEDLINE | ID: mdl-36858238

RESUMEN

Filter-feeder organisms such as oyster and mussels are exposed to particles like microplastics (MPs). Although widely used to monitor MPs contamination, little is known about their performance as sentinels, which are biological monitors accumulating contaminants without significant adverse effects. This study comparatively evaluated the quantitative and qualitative accumulation of MPs by oysters (Crassostrea brasiliana) and mussels (Perna perna) along a gradient of contamination in a highly urbanized estuarine system of Brazil. In the most contaminated site, both species presented the worst status of nutrition and health, and also one of the highest MPs levels reported for molluscs to date (up to 44.1 particles·g-1). Despite some inter-specific differences, oysters and mussels were suitable and showed an equivalent performance as sentinels, reflecting the gradient condition demonstrated for other contaminants in the region. The similarity in MPs accumulation was also observed for qualitative aspects (polymer composition, sizes, shapes and colors). Particles were mostly <1000 µm, fibrous, colorless and composed by cellulose and polymethyl methacrylate (PMMA). Thus, despite small variations, the usage of C. brasiliana and P. perna is recommended and provides reliable information for environmental levels of microplastics.


Asunto(s)
Crassostrea , Contaminantes Químicos del Agua , Animales , Microplásticos , Plásticos , Contaminantes Químicos del Agua/análisis , Alimentos Marinos/análisis , Monitoreo del Ambiente
19.
Rev Bras Farmacogn ; 33(2): 288-299, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-36908300

RESUMEN

Brazil is one of the largest propolis producers in the world. Propolis is produced by bees from plant exudates and tissues, leading to many variations in the types of propolis. Generally, Brazilian propolis types are green, brown, and red. Despite not being the main research focus as the green and red propolis, brown propolis is the second most produced propolis type in Brazil and has tremendous economic and medicinal importance. Propolis has drawn attention with the rise in the search for healthier lifestyles, functional foods, biocosmetics, and natural products as therapeutic sources. This review covers the main chemical constituents identified in different types of Brazilian brown propolis, and their botanical sources, chemistry, and biological activities. The economic aspect of brown propolis is also presented. There are many gaps to be filled for brown propolis regarding the development of analytical methods, and quality control to allow its standardization, limiting its applicability in the food and pharmaceutical industries. Future perspectives regarding brown propolis research were discussed, especially biological activities, to support the medicinal uses of different types of brown propolis. Supplementary Information: The online version contains supplementary material available at 10.1007/s43450-023-00374-x.

20.
Biomed Chromatogr ; 37(8): e5634, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-36977284

RESUMEN

Propolis is a natural product of great economic and pharmacological importance. The flora surrounding the bee communities is a determining factor in the composition of propolis and therefore in its biological and medicinal properties. Brown propolis is one of the most important types of propolis in Brazil, produced in the southeastern region. The ethanolic extract of a brown propolis sample from Minas Gerais state was chemically characterized for the subsequent development of a RP-HPLC method, validated according to the standards of regulatory agencies. The leishmanicidal activity of this extract was assessed. The brown propolis was characterized by the presence of chemical markers reported on green propolis such as ferulic acid, coumaric acid, caffeic acid, cinnamic acid, baccharin, artepillin and drupanin, indicating a probable origin on Baccharis dracunculifolia. The developed method agreed with the parameters established by the validation guidelines, then proved to be reliable for the analysis of this type of propolis. The brown propolis displayed significant activity against Leishmania amazonensis with IC50 values of 1.8 and 2.4 µg/ml against the promastigote and amastigote forms, respectively. The studied propolis exhibited promising evidence for use as a natural source against L. amazonensis.


Asunto(s)
Própolis , Própolis/farmacología , Própolis/química , Brasil , Cromatografía Líquida de Alta Presión , Extractos Vegetales/química , Estándares de Referencia
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