Field-realistic neonicotinoid exposure has sub-lethal effects on non-Apis bees: A meta-analysis.

Neonicotinoid insecticides can have sub-lethal effects on bees which has led to calls from conservationists for a global ban. In contrast, agrochemical companies argue that neonicotinoids do not harm honeybees at field-realistic levels. However, the focus on honeybees neglects the potential impact on other bee species. We conducted a meta-analysis to assess whether field-realistic neonicotinoid exposure has sub-lethal effects on non-Apis bees. We extracted data from 53 papers (212 effects sizes) and found that it largely consisted of two genera: bumblebees (Bombus) and mason bees (Osmia), highlighting a substantial taxonomic knowledge gap. Neonicotinoid exposure negatively affected reproductive output across all bees and impaired bumblebee colony growth and foraging. Neonicotinoids also reduced Bombus, but not Osmia, individual development (growth and body size). Our results suggest that restrictions on neonicotinoids should benefit bee populations and highlight that the current regulatory process does not safeguard pollinators from the unwanted consequences of insecticide use.

Measuring foraging preferences in bumble bees: a comparison of popular laboratory methods and a test for sucrose preferences following neonicotinoid exposure.

Animals develop food preferences based on taste, nutritional quality and to avoid environmental toxins. Yet, measuring preferences in an experimental setting can be challenging since ecologically realistic assays can be time consuming, while simplified assays may not capture natural sampling behavior. Field realism is a particular challenge when studying behavioral responses to environmental toxins in lab-based assays, given that toxins can themselves impact sampling behavior, masking our ability to detect preferences. We address these challenges by comparing different experimental methods for measuring sucrose concentration preference in bumble bees (Bombus impatiens), evaluating the utility of two preference chamber-based methods (ad libitum versus a novel restricted-sampling assay) in replicating bees' preferences when they fly freely between artificial flowers in a foraging arena. We find that the restricted-sampling method matched a free-flying scenario more closely than the ad libitum protocol, and we advocate for expanded use of this approach, given its ease of implementation. We then performed a second experiment using the new protocol to ask whether consuming the neonicotinoid pesticide imidacloprid, known to suppress feeding motivation, interfered with the expression of sucrose preferences. After consuming imidacloprid, bees were less likely to choose the higher-quality sucrose even as they gained experience with both options. Thus, we provide evidence that pesticides interfere with bees' ability to discriminate between floral rewards that differ in value. This work highlights a simple protocol for assessing realistic foraging preferences in bees and provides an efficient way for researchers to measure the impacts of anthropogenic factors on preference expression.

Microbes and pollinator behavior in the floral marketplace.

Pollinator foraging decisions shape microbial dispersal, and microbes change floral phenotypes in ways perceivable by pollinators. Yet, the role microbes play in the cognitive ecology of pollination is relatively unexplored. Reviewing recent literature on floral microbial ecology and pollinator behavior, we advocate for further integration between these two fields. Insights into pollinator learning, memory, and decision-making can help explain their responses to microbially-altered floral phenotypes. Specifically, considering how pollinators forage for multiple nutrients, cope with uncertainty, structure foraging bouts, and move through their environment could inform predictions about microbial dispersal within plant communities. We highlight how behavior connects microbial changes in floral phenotype to downstream effects on both microbial dispersal and plant fitness.

Asynchronous range shifts drive alpine plant-pollinator interactions and reduce plant fitness.

Climate change is driving species' range shifts, which are in turn disrupting species interactions due to species-specific differences in their abilities to migrate in response to climate. We evaluated the consequences of asynchronous range shifts in an alpine plant-pollinator community by transplanting replicated alpine meadow turfs downslope along an elevational gradient thereby introducing them to warmer climates and novel plant and pollinator communities. We asked how these novel plant-pollinator interactions affect plant reproduction. We found that pollinator communities differed substantially across the elevation/temperature gradient, suggesting that these plants will likely interact with different pollinator communities with warming climate. Contrary to the expectation that floral visitation would increase monotonically with warmer temperatures at lower elevations, visitation rate to the transplanted communities peaked under intermediate warming at midelevation sites. In contrast, visitation rate generally increased with temperature for the local, lower elevation plant communities surrounding the experimental alpine turfs. For two of three focal plant species in the transplanted high-elevation community, reproduction declined at warmer sites. For these species, reproduction appears to be dependent on pollinator identity such that reduced reproduction may be attributable to decreased visitation from key pollinator species, such as bumble bees, at warmer sites. Reproduction in the third focal species appears to be primarily driven by overall pollinator visitation rate, regardless of pollinator identity. Taken together, the results suggest climate warming can indirectly affect plant reproduction via changes in plant-pollinator interactions. More broadly, the experiment provides a case study for predicting the outcome of novel species interactions formed under changing climates.

Consequences of secondary nectar robbing for male components of plant reproduction.

PREMISE OF THE STUDY: Organisms engage in multiple species interactions simultaneously. While pollination studies generally focus on plants and pollinators exclusively, secondary robbing, a behavior that requires other species (primary robbers) to first create access holes in corollas, is common. It has been shown that secondary robbing can reduce plants' female fitness; however, we lack knowledge about its impact on male plant fitness. METHODS: We experimentally simulated primary and secondary robbing in the monocarpic perennial Ipomopsis aggregata (Polemoniaceae), quantifying indirect effects on pollinator-mediated pollen (dye) donation. We also assessed whether continual nectar removal via the floral opening has similar effects on hummingbird-pollinator behavior as continual secondary robbing through robber holes. KEY RESULTS: We found no significant indirect effects of secondary robbing on a component of Ipomopsis male fitness. Although robbing did reduce pollen (dye) donation due to avoidance of robbed plants by pollinating hummingbirds, pollen donation did not differ between the two robbing treatments. The effects of secondary robbing on hummingbird behavior resembled effects of chronic nectar removal by pollinators. Our results indicate that hummingbird pollinators may use a combination of cues, including cues given by the presence or absence of nectar, to make foraging decisions. CONCLUSIONS: Combined with prior research, this study suggests that secondary robbing is less costly to a component of male fitness than to female fitness in Ipomopsis, broadening our knowledge of the overall costs of mutualism exploitation to total plant fitness.

Active pollinator choice by Heliconia 'fits the bill'.

A new study documents that a tropical plant only reproduces when pollen has been deposited by a visitor capable of extracting nectar from its deep flowers. Large, long-billed hummingbirds generally carry greater quantities of, and more genetically diverse, pollen. Thus, plants can exert more active partner choice than previously considered possible.