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J Infect Dev Ctries ; 13(11): 1029-1037, 2019 11 30.
Artículo en Inglés | MEDLINE | ID: mdl-32087075

RESUMEN

INTRODUCTION: The challenges related to the diagnosis of sexually transmitted infections present more complex factors in remote and hard-to-reach areas. The use of self-collection devices that facilitate the obtaining of a biological sample with high quality for sensitive molecular tests have been examined. This study aimed to evaluate the performance and acceptance of the Evalyn® Brush (Rovers® Medical Devices) for detection of T. vaginalis among women living in the riverside communities of Amazonas, Brazil. METHODOLOGY: The study included 300 riverside women. They received instructions for self-collection, carried out the task, and then answered a questionnaire on the use of the device. T. vaginalis was detected by Polymerase Chain Reaction, using primers TVK3/TVK7. RESULTS: The mean age of the women was 35.8 years, and most of them presented low schooling, low income, agricultural activity and lived in a marital union. All samples were positive for human genomic DNA (100%) and the prevalence of T. vaginalis infection was 5.6% (n = 17). Of the 300 women, 293 (97.7%) indicated that they liked the use of the device, 287 (95.7%) reported having had no difficulty in handling it, 265 (88.3%) did not feel any type of discomfort and 228 (76%) said they preferred the self-collection to the collection made by the professional, mainly due to privacy and comfort. CONCLUSIONS: The Evalyn® Brush proved reliable as a device for the collection of biological samples for molecular analysis and was well-accepted by women. Its use can be indicated in remote and hard to reach places.


Asunto(s)
Manejo de Especímenes/instrumentación , Vaginitis por Trichomonas/parasitología , Adulto , Anciano , Brasil/epidemiología , Estudios Transversales , Diseño de Equipo , Femenino , Humanos , Persona de Mediana Edad , Prevalencia , Autocuidado , Parejas Sexuales , Manejo de Especímenes/métodos , Vaginitis por Trichomonas/epidemiología , Trichomonas vaginalis/genética , Trichomonas vaginalis/aislamiento & purificación , Adulto Joven
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