Diversity of Ladybird Beetles (Coleoptera: Coccinellidae) in Tenerife and La Gomera (Canary Islands): The Role of Size and Other Island Characteristics.

This paper provides new data on the ladybird beetles (Coccinellidae) from two islands in the Canary archipelago: Tenerife, the largest island, and La Gomera, the second smallest. As they clearly differ in size but are similar in location and geological age, they are a suitable model for testing the species-area relationship. Our study shows that, in line with this main assumption of the theory of island biogeography, clearly more species occur on a large island (Tenerife) than on a small one (La Gomera). The field surveys documented the occurrence of 35 ladybird species on Tenerife (including 5 not previously reported from this island) and of 20 species on La Gomera (2 species new to the island). Coelopterus sp. collected on Tenerife (a single female that could not be identified to species) is the first record of this genus for the whole Canary Islands. Taking our data and previously published records into account, 47 species of Coccinellidae are known to occur on Tenerife and 26 species on La Gomera. Tenerife has by far the richest ladybird fauna of all the Canary Islands (the next in line, Gran Canaria, has 41 recorded species), but it also has the highest number of non-native ladybird species. All of the ten non-native species recorded in the Canary Islands are found on Tenerife, and for most of them, Tenerife was the island of their first appearance in the archipelago. This island, much more distant from the mainland than the other relatively large islands (Fuerteventura, Lanzarote), appears to be the main recipient of ladybirds immigrating to the Canary Islands. Tenerife can play this role probably because of its great habitat diversity and altitude variation, as well as intensive tourism and trade-related transport.

The Diversity of Small Mammals along a Large River Valley Revealed from Pellets of Tawny Owl Strix aluco.

The Vistula River is one of the largest European semi-natural rivers of high ecological value that functions as an ecological corridor. To assess the structure of small mammal communities along the Vistula River, an analysis of the diet of an opportunistic predator, the tawny owl Strix aluco, was used. A total of 6355 individuals of 19 species were found, including 5 soricomorph species, 12 rodents, 1 carnivore, and 1 bat species. Tawny owls most frequently caught Apodemus agrarius, Clethrionomys glareolus, Apodemus flavicollis, and Microtus arvalis. Rodents dominated small mammal communities (90%), followed by soricomorphs (8%), and the share of Chiroptera was significant (2%). Using Ward's method in cluster analysis, three clusters of sites with similar mammal communities were identified. The cluster that included 17 study sites with the dominance of agriculture habitats was inhabited by diverse mammal communities with a high number of species. In the cluster composed of three suburban forest sites, mammal communities had the lowest diversity, although the high species richness and the highest shares of the forest species (A. flavicollis, C. glareolus, and Nyctalus noctula). Mammal communities in the cluster were composed of three urban sites were dominated by A. agrarius and M. arvalis. The study indicates the high species richness of small mammals in floodplains of the Vistula River and the adjacent areas in central Poland. The floodplain offers suitable habitats for species associated with forests, water bodies, agricultural land, and developed areas. The data collected confirms earlier model predictions about the presence of well-connected local populations of forest mammals along the Vistula River.

The Ladybird Beetles (Coleoptera: Coccinellidae) of La Palma.

This paper provides new data on the ladybird beetles (Coccinellidae) of La Palma, one of the western islands of the Canarian archipelago. The field survey of 54 study sites resulted in recording 2494 ladybird individuals belonging to 26 species. Seven of the species recorded were new to La Palma, including two, Harmonia quadripunctata (Pontoppidan) and Nephus reunioni (Fürsch), which were not registered so far on any of the Canary Islands. Novius conicollis (Korschefsky) is synonymized with N. cruentatus (Mulsant). Taking our survey and literature reports into account, a total of at least 35 species of Coccinellidae have so far been recorded on La Palma. This richness in species is lower compared to that of the central islands of the Canarian archipelago, Gran Canaria (42 species) and Tenerife (41 species), but higher than that of the remaining four islands (between 22 and 27 species). The detection of two alien species new to La Palma, Nephaspis bicolor Gordon and Nephus reunioni (Fürsch), confirms earlier observations that colonization of the Canary Islands by ladybird species of exotic origins seems to be a frequent phenomenon.

First Evidence of Microplastic Occurrence in the Marine and Freshwater Environments in a Remote Polar Region of the Kola Peninsula and a Correlation with Human Presence.

Microplastics (MPs) have even been detected in remote environments, including high-latitude regions, where human activities are restricted or strongly limited. We investigated the surface water of the bays of the Barents Sea and the freshwater lakes that are located close to and several kilometers from a year-round resident field station in the remote tundra region of the Kola Peninsula. The microplastics' presence in aquatic environments in this region has not been indicated yet. Microplastics were detected in all samples collected from the Barents Sea (<4800 items·m-3) and the lakes (<3900 items·m-3). Fibers made from polyethylene terephthalate (PET)-the most common thermoplastic polymer of the polyester family-and semi-synthetic cellulosic rayon were the most dominant. This indicated that the source of fiber contamination may come from protective clothes, ropes, ship equipment, and fishing nets. Small microplastics can spread through current and atmospheric transport. The Norwegian Current is likely responsible for the lack of correlations found between MP contamination and the distance from the field station between the studied bays of the Barents Sea. On the contrary, a significant correlation with human presence was observed in the concentration of microfibers in the water of the tundra lakes. The number of MP fibers decreased with an increase in the distance from the field station. This is the first study, to the best of our knowledge, that reports such a correlation in a remote region. We also discuss implications for animals. Our results show that even the most isolated ecosystems are not free from microplastic pollution.

Hesperomyces (Fungi, Ascomycota) associated with Hyperaspis ladybirds (Coleoptera, Coccinellidae): Rethinking host specificity.

Laboulbeniales (Ascomycota, Laboulbeniomycetes) are biotrophic microfungi always attached to the exoskeleton of their arthropod hosts. They do not form hyphae or a mycelium; instead, they undergo determinate growth, developing from a two-celled ascospore to form a multicellular thallus. Hesperomyces virescens has been reported on over 30 species of ladybirds (Coleoptera, Coccinellidae); in reality, it represents a complex of species, presumably segregated by host genus association. In this study, we report on Hesperomyces thalli on Hyperaspis vinciguerrae from the Canary Islands and compare them with the Hesperomyces hyperaspidis described on Hyperaspis sp. from Trinidad. We generated the sequences of the internal transcribed spacer (ITS) region, the large subunit (LSU) nuclear ribosomal RNA gene, and the minichromosome maintenance complex component 7 (MCM7) protein-coding gene. Our phylogenetic reconstruction of Hesperomyces based on a concatenated ITS-LSU-MCM7 dataset revealed Hesperomyces sp. ex Hy. vinciguerrae as a member of the He. virescens species complex distinct from He. virescens sensu stricto (s.s.). It also revealed that the Hesperomyces sp. ex Chilocorus bipustulatus from Algeria is different from He. virescens s.s., which is associated with Chilocorus stigma from the USA. This suggests that the species of Hesperomyces are not solely segregated by host association, but that there is also a biogeographical component involved. Based on these data, we refrained from referring our material from Hy. vinciguerrae to He. hyperaspidis. Finally, we discuss the usefulness of MCM7 as a useful marker for species delimitation in Hesperomyces.

Phylogenetic Relatedness within the Internally Brooding Sea Anemones from the Arctic-Boreal Region.

Phylogenetic analyses based on mitochondrial 16S rDNA, nuclear 28S rDNA, and morphological and ecological traits of Aulactinia, Urticina and Cribrinopsis sea anemones inhabiting the Arctic-boreal region indicate discordances between trees derived from molecular sequences and those based on morphological traits. Nuclear genes were more informative than mitochondrial and morphological datasets. Our findings indicate that 16S rDNA has limited applicability for phylogenetic analyses at lower taxonomic levels and can only be used for distinction of families. Although 28S rDNA allowed for the classification of distinct genera, it could not confirm that species of Urticina and Cribrinopsis, which appeared to be closely related, were correctly separated into two different genera. The nuclear tree revealed inconsistencies between specimens belonging to European Urticina crassicornis and Pacific U. crassicornis; the latter seems to be a different species. In contrast to Pacific U. crassicornis, the specimens collected from different localities in the Barents Sea are on the same tree branch. The same was observed for specimens of Aulactinia stella. Both species brood their young internally. The dispersal of sea anemones with brooding juveniles seems to be less limited than expected and might be sufficient to settle habitats more than a thousand kilometers away.

Endemics Versus Newcomers: The Ladybird Beetle (Coleoptera: Coccinellidae) Fauna of Gran Canaria.

Research on the fauna of beetles (Coleoptera) of the Canary Islands has a long tradition, which enables tracking changes in their species composition and arrival of new species. In this paper, we provide new faunistic data on the ladybird beetles (Coccinellidae) recorded on Gran Canaria, one of the central islands of the archipelago, and then analyze available information on the Gran Canarian ladybird fauna from geographical and historical points of view. The field survey resulted in recording 1402 ladybird individuals belonging to 30 species. Ten of these species were new to Gran Canaria and three of them, Chilocorus bipustulatus (Linnaeus), Nephus bisignatus (Boheman), and Nephus ulbrichi Fürsch, had not previously been reported to be on any of the islands of the Canarian archipelago. Tetrabrachys tinerfensis (Hodgson) is synonymized with T. deserticola (Wollaston). Our survey and literature reports allowed us to recognize 42 species of Coccinellidae so far recorded on Gran Canaria. Seventeen of them (40%) belonged to the Canarian endemic and subendemic species, and 21 (50%) were newcomers and presumed newcomers. Colonization of Gran Canaria and other islands of the archipelago by ladybird species of various origins seems to be a frequent phenomenon that may pose a threat to the unique communities of the native Canarian species.

The contamination of inland waters by microplastic fibres under different anthropogenic pressure: Preliminary study in Central Europe (Poland).

The contamination of freshwater ecosystems with microfibres has not yet been studied in Poland. We analysed samples from a river and three lakes located in central and northeastern Poland. A significantly higher number of fibres were reported in the river, which runs through large cities, compared with the lake situated within the Landscape Park. Fibres smaller than 1.0 mm dominated, especially in the river where they constituted 39% of all fibres detected. We found more microplastics (⩽ 4930 fibres·m-3) by using a mesh size of 20 µm compared with other studies of inland waters. The use of Raman spectroscopy allowed us to identify conventional plastic polymers: polyethylene terephthalate, polyester and polyurethane. We estimated that up to 25 g of microplastic in the form of fibres might be in the lake water under the surface. We found microplastic fibres in Majcz Lake situated within the Masurian Landscape Park. This suggests that microfibres are carried by the wind and rain and enter freshwater isolated from sewage outlets. By using the control sample and an air-test of microfibres in the laboratory, we observed that there is a high probability of contamination with microplastic in the field samples (up to 30% of all fibres detected). The contamination risk noted from the samples cannot be ignored; this could be particularly important for analysis of microplastic in remote freshwater ecosystems.

The Coccinellidae (Coleoptera) from Fuerteventura, Canary Islands.

During this study, Coccinellidae were collected and observed at 25 sites located along the coast and inland of the island of Fuerteventura during 2016 and 2017. A total of 2825 Coccinellidae specimens belonging to 22 species was recorded, of which 11 species are newly recorded from Fuerteventura. In the case of three species taxonomical decisions are proposed. Scymnus incisus (Har. Lindberg, 1950) is transferred to the genus Nephus Mulsant and placed in the nominate subgenus as Nephus (Nephus) incisus (Har. Lindberg, 1950), comb. nov. Scymnus medanensis Eizaguirre, 2007, is redescribed and placed in the subgenus Pullus Mulsant. Coccinella algerica Kovár, 1977, is synonymized with Coccinella septempunctata Linnaeus, 1758, syn. nov. We propose retaining the taxonomic distinction of the North African and Canarian populations of this species as the subspecies Coccinella septempunctata algerica Kovár, stat. nov. Nephus peyerimhoffi (Sicard, 1923) is deleted from the list of ladybird beetles inhabiting the Canary Islands.

High efficiency protocol of DNA extraction from Micromys minutus mandibles from owl pellets: a tool for molecular research of cryptic mammal species.

Owl pellets have high potential as a source of DNA. However, this noninvasive method of collecting DNA is rarely used, and its methodological aspects are poorly understood. We investigated the methodology for DNA extraction and amplification from owl pellets containing the smallest European rodent-the Harvest mouse Micromys minutus-as an example. We used mandibles identified in owl pellets for mitochondrial and nuclear DNA amplification. For DNA extraction, we tested two commercial protocols and utilized a protocol being a combination of two commercial kits which ensured high efficiency of DNA extraction. Additionally, we recorded that the amount of DNA was five times higher in extracts from teeth as compared to DNA extracts from jawbones derived from the same mandible. The quantity of DNA was significantly positively correlated with biological sample weight; however, the age of the pellet remains had an impact on the level of inhibition. We recorded inhibition in 40 % of mtDNA extracts derived from pellets older than 150 months, whereas in DNA extracts from pellets younger than 80 months, we did not observe a negative impact of inhibition on PCR efficiency. The amplification success rate was 89.9 % for the mitochondrial fragment and 39.4 % in the case of the nuclear fragment. We observed partial degradation of DNA evidenced by the fact that the longest fragments that we were able to amplify in the case of mtDNA were 450 and 200 bp for nuDNA. The study shows that pellets can be considered as a source of DNA and have high potential for molecular research in the case of threatened species and species that are difficult to study using standard field techniques.

Ranking landscape development scenarios affecting natterjack toad (Bufo calamita) population dynamics in Central Poland.

When data are limited it is difficult for conservation managers to assess alternative management scenarios and make decisions. The natterjack toad (Bufo calamita) is declining at the edges of its distribution range in Europe and little is known about its current distribution and abundance in Poland. Although different landscape management plans for central Poland exist, it is unclear to what extent they impact this species. Based on these plans, we investigated how four alternative landscape development scenarios would affect the total carrying capacity and population dynamics of the natterjack toad. To facilitate decision-making, we first ranked the scenarios according to their total carrying capacity. We used the software RAMAS GIS to determine the size and location of habitat patches in the landscape. The estimated carrying capacities were very similar for each scenario, and clear ranking was not possible. Only the reforestation scenario showed a marked loss in carrying capacity. We therefore simulated metapopulation dynamics with RAMAS taking into account dynamical processes such as reproduction and dispersal and ranked the scenarios according to the resulting species abundance. In this case, we could clearly rank the development scenarios. We identified road mortality of adults as a key process governing the dynamics and separating the different scenarios. The renaturalisation scenario clearly ranked highest due to its decreased road mortality. Taken together our results suggest that road infrastructure development might be much more important for natterjack toad conservation than changes in the amount of habitat in the semi-natural river valley. We gained these insights by considering both the resulting metapopulation structure and dynamics in the form of a PVA. We conclude that the consideration of dynamic processes in amphibian conservation management may be indispensable for ranking management scenarios.

Habitat correlates of the Eurasian otter Lutra lutra recolonizing Central Poland.

The increase in Eurasian otter Lutra lutra populations in their natural range and recolonization processes are recently observed in several European countries. We address the process of otter recolonization and habitat utilization in Central Poland over 14 years. Field surveys in 1998 and 2007 documented increase in occurrence of the species. The frequency of positive sites denoted 15 % in 1993, 38 % in 1998, and 89 % in 2007. Otter occurrence at study sites was positively affected by river width while negatively affected by presence of buildings at the site and river regulation. During the most intensive colonization process in the 1990s, the habitat preferences of the otter did not change. However, the sites inhabited by otters after 1998 were characterized by lower river width and tree cover and were more often located on regulated river sections, suggesting change in habitat tolerance during expansion. The otter abundance in transformed habitats is a result of increasing population numbers and the necessity to inhabit suboptimal sections of watercourses. Thus, it seems that presence-absence data for otter populations cannot be considered a reliable indicator of habitat quality, being depended of the population density.

[Semi-aquatic animals as a source of water contamination with Cryptosporidium and Giardia]. / Zwierzeta ziemnowodne jako zródlo zanieczyszczenia wody Cryptosporidium i Giardia.

Cryptosporidium and Giardia spp. are parasitic protozoa localized in the alimentary tract of many animal species and humans. Each of these parasite species produces very resistant invasive forms (cysts and oocysts) excreted to the environment with feces of infected hosts. Water contaminated with cysts/oocysts constitutes one of the main transmission routes and is responsible for the majority of infections in humans. Cryptosporidium and Giardia spp. were found in many different species of animals, including livestock, pets and free living animals. The aim of our study was to determine the prevalence of these protozoa in selected species of semi-aquatic mammals and to estimate their role in water contamination. In years 1996-98 the prevalence of Cryptosporidium and Giardia infections was high in muskrats (Ondatra zibethicus) (58 and 87%, respectively). The origin of animals (farmed or free living) affected the prevalence of both parasites in European beavers (Castor fiber). The prevalence of infection increased in second period of study and was 4 and 19% for Cryptosporidium and 0 and 8% for Giardia spp. in the two studied periods, respectively. Both parasite species were also identified in water vole (Arvicola terrestris) and rat (Rattus norvegicus).