[The relationship between male semen parameters and sperm DNA fragment index with the age].

To investigate the relationship between male semen parameters and sperm DNA fragment index with age. Adopt cross-sectional sampling survey design, 3 203 male patients who visited the Department of Reproductive Andrology in the Third Affiliated Hospital of Zhengzhou University from January 2019 to June 2021 were selected as subjects. Age range is 18-57 years, with the median age of 30 years. Through quartile regression analysis, the correlation between age and different male semen parameters and DNA fragment index (DFI) was presented. The study population was divided into ≤30 years old group and >30 years old group, and the correlation between age and semen volume, sperm concentration, total sperm count, progressive motility, total motility, percentage of normal sperm and DFI level were compared and analyzed. The results showed that there were significant differences in progressive motility, total motility and DFI level among different age groups (χ2=-4.608, -4.604, -7.719,P all <0.05), but there was no significant difference in semen volume, sperm concentration, total sperm count and percentage of normal sperm (χ2=-1.712, -1.203, -0.149, -0.175,P all >0.05). In the>30 years old age group, there was a very weak negative correlation between male age and semen volume, progressive motility and total motility (r=-0.137, -0.101 and -0.056, P all <0.05). There was a very weak positive correlation between male age and sperm concentration and sperm DFI level (r=0.061, 0.190, P all <0.05), while there was no correlation between male age and total sperm count and percentage of normal sperm (r=-0.018, -0.016,P all >0.05). In conclusion, with the increase of age, especially after the age of 30, semen volume, progressive motility and total motility decreased, while sperm concentration and DFI level increased, and semen quality decreased.

[The Rice OsDUF810 Family: OsDUF810.7 May be Involved in the Tolerance to Salt and Drought].

With the advance of sequencing technology, the number of sequenced plant genomes has been rapidly increasing. However, understanding of the gene function in these sequenced genomes lags far behind; as a result, many coding plant sequences in public databases are annotated as proteins with domains of unknown function (DUF). Function of a protein family DUF810 in rice is not known. In this study, we analysed seven members of OsDU810 (OsDUF810.1-OsDUF810.7) family with three distinct motifs in rice Nipponbare. By phylogenetic analysis, OsDUF810 proteins fall into three major groups (I, II, III). Expression patterns of the seven corresponding OsDUF810 protein-encoding genes in 15 different rice tissues vary. Under drought, salt, cold and heat stress conditions and ABA treatment, the expression of OsDUF810.7 significantly increases. Overexpression of this protein in E. coli lead to a significant enhancement of catalase (CAT) and peroxidase (POD) activities, and improved bacterial resistance to salt and drought.

Prevalence and genotype distribution of human papillomavirus in women in the Henan Province.

We studied human papillomavirus (HPV) prevalence and genotype distribution among women in the Henan Province to provide epidemiological data as a means of preventing cervical cancer and developing a vaccine. A total of 14,873 samples were genotyped by using polymerase chain reaction reverse dot-blot. The overall HPV-positive rate in the sample was 23.98% (3566/14873), of which 69.01% (2461/3566) were infected with high-risk HPV types and 17.33% (618/3566) with low-risk types. Eighteen high-risk HPV types were detected; HPV 16 (16.73%) was the most common, followed by 58 (10.17%), 52 (9.11%), 56 (6.48%), 66 (5.76%), 33 (4.74%), 68 (3.92%), 31 (3.60%), 53 (3.13%), 59 (3.00%), 35 (2.53%), 51 (2.00%), 73 (1.08%), 45 (0.94%), 83 (0.84%), 39 (0.69%), 18 (0.61%), and MM4 (0.04%). Four low-risk HPV types were detected; HPV 43 (11.34%) was the most common, followed by 6 (5.17%), 42 (4.76%), and 11 (3.35%). Type 44 was not detected. Among the women positive for HPV, 71.17% (2538/3566) had a single type of infection; of these, 54.66% (1949/3566) had high-risk and 16.52% (589/3566) had low-risk infections. A total of 28.83% (1028/3566) had multiple HPV infections, of which 20.11% (717/3566) had double HPV infections. One peak in HPV prevalence occurred among women younger than age 25; a second peak occurred among women older than age 55. The overall prevalence of HPV infection in the Henan Province was 23.98%, of which the most common type was high-risk HPV and a single type of infection. The leading genotypes were HPV 16, 43, 58, 52, and 56.

Antiproliferative and teratogenic activities of the bishemisuccinates of 7 alpha-hydroxycholesterol and 7 beta-hydroxycholesterol.

1. The bishemisuccinates of 7 alpha-hydroxycholesterol and 7 beta-hydroxycholesterol induced a dose-dependent decrease in the incorporation of [3H]thymidine into fibroblast cell line (3T3), macrophage-like cell line (P388D1) and rat hepatoma cell line (H35). Concomitantly there was a reduction in cell viability. 2. The 3T3 cells were less susceptible than P388D1 and H35 cells. 3. The aforementioned derivatives of 7 alpha-hydroxycholesterol and 7 beta-hydroxycholesterol produced malformations in cultured embryos including abnormalities in yolk sac circulation, heartbeat, body axis, otic vesicle, branchial apparatus, cranial neural tube and forelimb bud. Axial length and somite number were reduced.

Hydatid disease of bone.

Twenty cases of hydatid disease of bone revealed by X-ray examination are presented. The vertebrae were involved in 12 of the cases, the pelvis in four, both the vertebrae and the pelvis in one, the ribs in two and the femur in one. These cases constituted 1.3% of all cases of hydatid disease observed in our hospital during the period 1957-80. The features of the disease are discussed in terms of plain radiographs, myelography and tomography. Tomographic studies have proved of value in differentiating the disorder from other diseases.