Adherence to healthy dietary patterns and its association with physical fitness in military personnel.

INTRODUCTION: Poor nutritional status might impact in unintentional weight loss and decreased muscle mass and strength. The levels of physical fitness (PF) is a predictor of chronic diseases in future years. Also, evaluation of fitness is an essential factor for duty in occupations which are sensitive to time and emergency reaction. Since there is no literature that study the association between dietary patterns and the PF level in physically strenuous occupations, the aim of this study was to examine the relationship between dietary patterns and the PF level in military staffs. METHODS: This cross-sectional study was carried out in military zones of Iran. Typical dietary intakes were assessed by using a semi-quantitative food-frequency questionnaire. Mediterranean diet (MD) score and healthy diet indicator (HDI) score were calculated. PF tests were designed to test the muscular strength, endurance and cardiovascular respiratory fitness (CRF). RESULTS: Mean age of participants was 33.03±4.65 in the study. Referring to the performance in the PF tests, there was a correlation between tertiles of MD (OR 4.37, p=0.048) and HDI (OR 5.21, p=0.019) scores and suitable PF level in the crude and confounder adjusted models. Also, a negative relationship was found between body mass index and waist circumference with fitness score. CONCLUSIONS: We concluded that accompanying a medium or high adherence to a healthy dietary pattern will have an impact on good level of PF and will result in a better working power and long-term health consequences among physically strenuous occupations.

Acute localized exanthematous pustulosis caused by enoxaparin.

Notably, enoxaparin has not been described to cause acute localized exanthematous pustulosis (ALEP). Herein, we present a case of a woman with a cutaneous drug reaction consistent with ALEP that occurred after enoxaparin. This case highlights enoxaprin as a novel causative agent for this type of drug reaction.

Acoustogalvanic Effect in Dirac and Weyl Semimetals.

The acoustogalvanic effect is proposed as a nonlinear mechanism to generate a direct electric current by passing acoustic waves in Dirac and Weyl semimetals. Unlike the standard acoustoelectric effect, which relies on the sound-induced deformation potential and the corresponding electric field, the acoustogalvanic one originates from the pseudoelectromagnetic fields, which are not subject to screening. The longitudinal acoustogalvanic current scales at least quadratically with the relaxation time, which is in contrast to the photogalvanic current where the scaling is linear. Because of the interplay of pseudoelectric and pseudomagnetic fields, the current could show a nontrivial dependence on the direction of sound wave propagation. Being within the experimental reach, the effect can be utilized to probe dynamical deformations and corresponding pseudoelectromagnetic fields, which are yet to be experimentally observed in Weyl and Dirac semimetals.

Development a smart edible nanocomposite based on mucilage of Melissa officinalis seed/montmorillonite (MMT)/curcumin.

The aim of the present study was, first to fabricate Melissa officinalis seed gum (MOSG)-based films incorporated with montmorillonite (MMT) at various concentrations and subsequently, evaluate of the physicochemical properties of the developed films. Afterward, the anti-bacterial and anti-mold activities of the developed nanocomposites were assessed. Finally, curcumin was incorporated into formulation of the fabricated film at optimal condition to sense pH changes. Incorporating MMT nanoparticles into MOSG-based films could reduce the thickness, water solubility and moisture content of the samples. Tensile strength (TS) and elongation at break (EB) values significantly increased with increase of MMT concentration. The nanoparticle addition specifically at higher levels resulted in increase of agglomeration. Neither anti-mold and nor anti-microbial activity were observed for the tested nanocomposites. The films containing curcumin had good antibacterial and anti-mold activities and can be used as smart package due to their ability to sense the pH change.

The bisphosphonate, zoledronic acid, induces apoptosis of breast cancer cells: evidence for synergy with paclitaxel.

Bisphosphonates are well established in the management of breast-cancer-induced bone disease. Recent studies have suggested that these compounds are effective in preventing the development of bone metastases. However, it is unclear whether this reflects an indirect effect via an inhibition of bone resorption or a direct anti-tumour effect. The breast cancer cell lines, MCF-7 and MDA-MB-231 cells were treated with increasing concentrations of the bisphosphonate, zoledronic acid, for varying time periods, in the presence or absence of paclitaxel. The effects of zoledronic acid were determined by assessing cell number and rate of apoptosis by evaluating changes in nuclear morphology and using a fluorescence nick translation assay. Zoledronic acid caused a dose- and time-dependent decrease in cell number (P< 0.001) and a concomitant increase in tumour cell apoptosis (P< 0.005). Short-term exposure to zoledronic acid was sufficient to cause a significant reduction in cell number and increase in apoptosis (P< 0.05). These effects could be prevented by incubation with geranyl geraniol, suggesting that zoledronic acid-induced apoptosis is mediated by inhibiting the mevalonate pathway. Treatment with zoledronic acid and clinically achievable concentrations of paclitaxel resulted in a 4-5-fold increase in tumour cell apoptosis (P< 0.02). Isobologram analysis revealed synergistic effects on tumour cell number and apoptosis when zoledronic acid and paclitaxel were combined. Short-term treatment with zoledronic acid, which closely resembles the clinical setting, has a clear anti-tumour effect on breast cancer cells. Importantly, the commonly used anti-neoplastic agent, paclitaxel, potentiates the anti-tumour effects of zoledronic acid. These data suggest that, in addition to inhibiting bone resorption, zoledronic acid has a direct anti-tumour activity on breast cancer cells in vitro.

Texturing and polishing. The final attempt at value control.

This article reviews methods for developing surface texture and controlling the luster of ceramic restorations. The authors discuss the characteristics of embryology and age, and profile characteristics of teeth that are vital in creating natural, anatomic restorations. A technique is proposed to enhance the scattering of light and the amount of luster desired in the restoration. The authors recommend hand polishing over autoglazing as a more efficient way to obtain the desired luster. The combination of developing the proper texture and luster is important for the overall value of the restoration.

Interindividual variability of coumarin 7-hydroxylation in a Turkish population.

One hundred healthy Turkish volunteers (70 male, 30 female) aged from 19 to 56 years were given 5 mg coumarin p.o. after an overnight fast. Urine samples were collected before and 2, 4 and 8 h after drug administration. The extent and rate of formation of 7-OH-coumarin (7OHC) was determined by the urinary excretion of the metabolite as measured with the fluorometric method. On average, 80% of 7OHC formed was excreted in 2 h. The total amount of 7OHC formed was 59.8% (21.5%) (mean and SD, n = 100, range 17-100%) of the given dose. The percentage of 7OHC excreted during the first 2 h compared with the 7OHC excretion at 8 h was a constant and stable individual characteristic for the rate of the formation of 7OHC ('2 h coumarin test'). Although four individuals had relatively slow coumarin test values (34-40%), no clear-cut polymorphism in the rate of 7OHC formation was found. However, 7OHC formation was lower in males and in cigarette smokers.

Evaluation of four methods for scoring cytoplasmic grains in the in vitro unscheduled DNA synthesis (UDS) assay.

The in vitro unscheduled DNA synthesis (UDS) assay measures DNA repair (incorporation of [3H]thymidine) following in vitro treatment of rat primary hepatocytes. The autoradiographic method was used to detect UDS by counting developed silver grains in the photographic emulsion overlaying nuclei and cytoplasmic areas of the hepatocytes. In this communication we report results using 4 scoring methods: (1) the 2 most heavily labeled cytoplasmic areas adjacent to the nucleus (our standard method), (2) the cytoplasmic area left of the nucleus, (3) the cytoplasmic areas left and right of the nucleus, and (4) 2 cytoplasmic areas whose positions were selected at random. Rat primary hepatocyte cultures treated with a medium control, a solvent control (dimethyl sulfoxide) and 5 known genotoxic chemicals (2-acetylaminofluorene, dimethylnitrosamine, diethylnitrosamine, methyl methanesulfonate and ethyl methanesulfonate) were scored using these 4 methods. The average or maximum cytoplasmic grain count was subtracted from the nuclear grain count to yield net grains/nucleus (NG). In general, NG counts for Methods 2, 3 and 4 were similar, although shifted about 3-10 grains higher than Method 1 for controls and most treated groups. Methods 2, 3 and 4 showed more experiment-to-experiment variability in sensitivity for detecting statistically significant increases in treated groups than did our standard method. Thus, the alternative methods afforded no consistent improvements in sensitivity or reduction of variability for this assay. Subtraction of the average or the highest cytoplasmic count had virtually no effect on the sensitivity of the assay, but simply requires an appropriate adjustment of the criteria for a positive response.

The effect of antiinflammatory drugs on plasma fibronectin.

When rats were injected with Freund's adjuvant to induce arthritis, systemic disease as measured by swelling of the noninjected paw, was paralleled by a 100% rise in plasma fibronectin as measured by electroimmunoassay. When arthritic rats were given daily oral doses of nonsteroidal antiinflammatory drugs (NSAID), swelling of the noninjected rear paw was significantly less than that of the untreated arthritic controls. However, in all cases, plasma fibronectin (Fn) levels remained high in drug treated arthritic rats. Whether the NSAID was aspirin (100 mg/kg), phenylbutazone (10, 30, or 100 mg/kg) or indomethacin (0.3, 1 or 3 mg/kg) the pattern remained the same--reduced paw volume and unchanged high plasma Fn levels. Fn levels also remained unaltered in normal animals treated with drugs alone. Though NSAID diminish inflammation, clinical studies have shown that they do not halt disease progression. Our report shows that NSAID also fail to alter production of high levels of plasma Fn.

Nascent and remnant lipoprotein turnover in rats: experimental design and simulation.

We have attempted to predict the kinetic behavior of the complex very low-density lipoprotein (VLDL; d less than 1.006) fraction in blood plasma of rats in the steady state. Specifically we proposed a simple model with two different kinds of nascent VLDL particles derived from the liver, one containing apoprotein B (PI/II) [apoB(PI/II)], the high-molecular-weight apoB, and the other, apoprotein B (PIII) [apoB(PIII)], the low-molecular-weight apoB. Two other particles, the corresponding remnants derived from the nascent VLDL particles were also included. Then a number of feasible in vivo tracer experiments were considered in which VLDL labeled in the apoB and/or triglyceride (TG) moieties would be injected into recipient rats and the kinetic behavior of the various compartments predicted by simulation analysis. In addition the kinetic behavior of products such as free fatty acids formed during hydrolysis of labeled TG fatty acids and liver TG derived from labeled circulating remnants was considered. Both the relative sizes of nascent and remnant particles and the extent of average hydrolysis of nascent VLDL-TG (before formation of a remnant particle) were considered in our analysis. On the basis of these predictions we have suggested a number of experimental approaches that should be helpful in defining the relative pool sizes and the turnover rates of each kind of particle in vivo.

Amiodarone kinetics after oral doses.

Amiodarone serum kinetics after single oral doses and after long-term therapy were investigated in patients with ventricular tachyarrhythmias. When amiodarone was given as a single oral dose (1400 to 1800 mg, n = 6), serum levels of amiodarone and its metabolite, measured by high-performance liquid chromatography, correlated (r = 0.69, P less than 0.01). Peak concentrations (amiodarone, 3 to 14 microgram/ml; metabolite, 0.7 microgram/ml) were attained in 4.9 +/- 1.2 hr. Using computer fits to the data, amiodarone mean elimination rate constant and half-life (t 1/2 e) were 0.128 +/- 0.063 hr-1 and 7.2 +/- 5.0 hr. In 12 patients given a mean dose of 1327 +/- 338 mg/day of amiodarone for 4.1 +/- 2.3 wk, mean serum amiodarone level was 3.84 +/- 2.92 microgram/ml (range 0.92 to 11.99); in three patients simultaneous determination of concentrations of amiodarone and its metabolite revealed that concentration of the latter was about 50% of that of the parent drug during long-term therapy. In four patients on maintenance therapy (400 to 800 mg/day, serum level 1.08 +/- 1.3 microgram/ml) drug was discontinued and serum amiodarone levels were determined serially. Serum drug disappearance followed a single exponential function with an elimination rate constant of 0.030 +/- 0.012 day-1 and t 1/2 e of 29 +/- 19 days. Our kinetic data are consistent with the long therapeutic amiodarone t 1/2 noted in the treatment of cardiac arrhythmias.

Very low density lipoprotein metabolism in domestic pigs.

Patterns of triacylglycerol (TG) turnover in plasma and liver, and the hepatic secretion of very density lipoprotein triacylglycerol (VLDL TG) into the circulation, have been studied in young Large White female pigs, using i.v. tracer [1,3-14C]- and [2-3H]glycerol. Serial measurements were made of plasma [14C]glycerol and [14C]glucose and of liver TG and plasma VLDL TG specific activities. In other studies VLDL TG obtained from a donor pig was reinjected into recipient animals to measure the early disappearance (dilution) of VLDL TG. Multicompartmental analysis revealed a mean rate of hepatic TG turnover somewhat slower than the rate of VLDL TG turnover, suggesting that almost all of the hepatic TG turnover was due to secretion of VLDL TG, and that the intestine probably contributed an appreciable part of the newly synthesized plasma VLDL TG. The t1/2 of reinjected VLDL TG, whether prepared by the most rapid possible techniques and reinjected immediately or stored for several days, was approximately 10 min. This was much faster than the t1/2 of the falling limb of the plasma VLDL TG curve seen after injection of labelled glycerol (t1/2 approximately 2 H). Thus, in these respects, the pig resembled all other species studied rather than human subjects as described by Farquhar et al.

Evaluation of glucose turnover, body mass and recycling with reversible and irreversible tracers.

1. Methods are presented for the calculation of rates of synthesis or loss, mean transit time and total body pool of compounds from specific-radioactivity curves, without assuming a multicompartmental model and without fitting the data by exponential expressions. The methods apply to the steady state after either single injection or continuous infusion of a labelled compound. 2. The use of irreversible and reversible tracers and the effects of recycling of carbon on the estimations of the parameters of glucose metabolism are discussed. Methods for quantitatively determining recycling of glucose carbon by the use of glucose doubly labelled with (14)C and (3)H are presented.

Effect of glucose ingestion on the metabolism of free fatty acids in human subjects.

The rate of appearance of (14)CO(2) in expired air after the injection of a single dose of NaH(14)CO(3) has been determined in normal individuals both in the fasted and fed states. These data were combined with previously obtained results on the rate of disappearance of injected palmitate-(14)C from the bloodstream, to give a multicompartmental analysis of free fatty acid oxidation and esterification. The results confirm that glucose feeding promptly inhibits the rate of free fatty acid oxidation to CO(2). The "irreversible disposal rate," or irreversible flux of free fatty acids from the plasma, was also consistently reduced by glucose feeding. The diminution in irreversible disposal, not accounted for entirely by reduction of direct oxidation, must indicate suppression of other disposal mechanisms, including net esterification of free fatty acids. An average drop of 49% in "net esterification" when glucose was given may be compared with the 65% inhibition of rapid free fatty acid oxidation.

Effect of glucose feeding on net transport of plasma free fatty acids.

The effect of a single glucose feeding upon the net inflow and outflow transport of plasma free fatty acids (FFA) has been studied in 75 unanesthetized rats. The animals were fasted for 22 +/- 2 hr; then 50 rats were refed 2 ml of 50% glucose by gastric intubation. At 0, 10-15, and 30-35 min after glucose refeeding, the rats were injected with palmitate-1-(14)C complexed to rat serum. The tracer dose included (131)I-labeled albumin. Plasma FFA concentration, (131)I concentration, and FFA-(14)C were measured at five time intervals after injection of the tracer dose. From these data the irreversible disposal rate, or net outflow transport, and the net inflow transport of plasma FFA were calculated. Estimations were based upon a special case of a general solution for measuring net inflow and outflow transport of a circulating metabolite. The general solution is independent of the number of compartments, how they are interconnected, the number of nonradioactive inflows, and where the inflows enter the system. Net inflow = net outflow transport = 7.6 micro eq/min in the fasted state and 3.5 micro eq/min in the new steady state that is reached 30-40 min after glucose refeeding. A very slight imbalance between the rates of net inflow and outflow transport could account for the rapid fall in plasma FFA concentration that results from a single glucose feeding. Theoretical and practical problems associated with studying inflow and outflow transport by means of the technique using a single injection of racer are discussed.