RESUMEN
Chromoblastomycosis is defined as a chronic cutaneous and subcutaneous fungal infection caused by melanized or brown-pigmented fungi. A 63-year-old man farmer showed on external and internal part of the right arm, a well-delimited verrucous and hyperkeratotic plaque, with atrophic and cicatricial areas. Direct examination of skin scrapings samples showed the presence of muriform cells, a classic feature of chromoblastomycosis. Fungal isolation was performed in Sabouraud dextrose agar, and dark olivaceous colonies were isolated. Skin biopsy samples were obtained for histopathological and molecular diagnosis. DNA extracted from both, paraffin-embedded skin biopsy samples and fungal colonies, was used for molecular identification by 18S-ITS1-5.8S-ITS2-28S rRNA amplification and sequencing. Fonsecaea pedrosoi was identified from paraffin-embedded skin samples and fungal colonies. A combined therapy with terbinafine and itraconazole, plus cryotherapy was applied with an important improvement. Herein, we report an impressive case of chromoblastomycosis due to Fonsecaea pedrosoi with a successful outcome.
Asunto(s)
Ascomicetos/aislamiento & purificación , Cromoblastomicosis/diagnóstico , Cromoblastomicosis/terapia , Antifúngicos/uso terapéutico , Ascomicetos/citología , Ascomicetos/efectos de los fármacos , Ascomicetos/genética , Cromoblastomicosis/patología , Terapia Combinada , Crioterapia , ADN de Hongos/genética , ADN Espaciador Ribosómico/genética , Genoma Fúngico/genética , Humanos , Itraconazol/uso terapéutico , Masculino , Persona de Mediana Edad , Análisis de Secuencia de ADN , Piel/microbiología , Piel/patología , Terbinafina/uso terapéutico , Resultado del TratamientoRESUMEN
We have determined absolute charge transfer and fragmentation cross sections in He2++C60 collisions in the impact-energy range 0.1-250 keV by using a combined experimental and theoretical approach. We have found that the cross sections for the formation of He+ and He0 are comparable in magnitude, which cannot be explained by the sole contribution of pure single and double electron capture but also by contribution of transfer-ionization processes that are important even at low impact energies. The results show that multifragmentation is important only at impact energies larger than 40 keV; at lower energies, sequential C2 evaporation is the dominant process.
RESUMEN
Anopheles sundaicus species A of the Southeast Asian A. sundaicus complex is formally named Anopheles epiroticus Linton & Harbach based on DNA sequence differentiation of the whole nuclear ITS2 region and a portion of both the cytochrome b and cytochrome c oxidase I mitochondrial genes. Detailed comparative morphological studies of the adult, larval and pupal stages did not reveal any differential or diagnostic differences that reliably distinguish A. epiroticus from A. sundaicus s.s. Information is provided on the bionomics and systematics of the new species.
Asunto(s)
Anopheles/clasificación , Anopheles/genética , Insectos Vectores/clasificación , Insectos Vectores/genética , Malaria/transmisión , Animales , Anopheles/parasitología , Asia Sudoriental , Secuencia de Bases , ADN/química , Femenino , Marcadores Genéticos , Masculino , Datos de Secuencia Molecular , Filogenia , Alineación de Secuencia , Especificidad de la EspecieRESUMEN
We present a combined theoretical and experimental study of charge transfer and dissociation in collisions of slow Li31(2+) clusters with Cs atoms. We provide a direct quantitative comparison between theory and experiment and show that good agreement is found only when the exact experimental time of flight and initial cluster temperature are taken into account in the theoretical modeling. We demonstrate the validity of the simple physical image that consists in explaining evaporation as resulting from a collisional energy deposit due to cluster electronic excitation during charge transfer.
