Asunto(s)
Aedes/efectos de los fármacos , Culex/efectos de los fármacos , Insecticidas/farmacología , Mimusops/química , Extractos Vegetales/farmacología , Animales , Insecticidas/aislamiento & purificación , Larva/efectos de los fármacos , Extractos Vegetales/aislamiento & purificación , Análisis de SupervivenciaRESUMEN
The present study was carried out to investigate the anti-inflammatory activities of bioactive secondary metabolites of Artemisia pallens Wall, an aromatic herb from family Asteraceae. The results provide evidence for the topical anti-inflammatory properties of Artemisia pallens Wall. The compounds were isolated from the acetone extract of the plant material. The isolates were tested on Swiss albino mice using 12-O-tetradecanoyl phorbol-13-acetate (TPA) induced ear edema. One of the molecules from the extract indicated potent anti-inflammatory activity equivalent to indometacin (CAS 53-86-1). Elucidation of the molecular structures by single crystal x-ray diffraction studies revealed the conformational differences that the six membered rings in both the molecules are at an angle of 28.79 degrees. Presence of hydroxy function for compound 2 may be responsible for higher potency.
Asunto(s)
Antiinflamatorios no Esteroideos/farmacología , Artemisia/química , Sesquiterpenos/química , Sesquiterpenos/farmacología , Acetona , Animales , Cromatografía Líquida de Alta Presión , Cristalografía por Rayos X , Edema/inducido químicamente , Edema/prevención & control , Femenino , Enlace de Hidrógeno , Indometacina/farmacología , Lactonas/química , Espectroscopía de Resonancia Magnética , Masculino , Ratones , Modelos Moleculares , Extractos Vegetales/química , Solventes , Acetato de Tetradecanoilforbol/farmacologíaRESUMEN
Herbs have been used for medicinal purposes for centuries. According to recent investigations, they may help reduce the risk of chronic diseases, cardiovascular disease, and cancer due to antioxidant properties, which in turn can be attributed to the various phytoconstituents. With this intention, evaluation of antioxidant activity was performed. Methanol extract of aerial parts of Artemisia pallens Wall was screened for its antioxidant activity due to phenolic and flavonoid contents, by employing radical scavenging assays; 2,2 -diphenyl, 1-picryl hydrazyl (DPPH) and nitric oxide. Ascorbic acid was used as a standard. Quantitative determination of phenols and flavonoids were carried out using spectrophotometric method. Total flavonoid content was determined as quercetin equivalent and total phenolic content was determined as pyrocatechol equivalent using Folin-Ciocalteu reagent. Plant produces more phenolic compounds than flavonoids. IC(50) value of methanol extract for DPPH free radical scavenging activity was found to be 292.7 µg, whereas for nitric oxide it was 204.61 µg. The result obtained in the present study indicates that the aerial parts of this plant are a rich source of natural antioxidants.