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Nanomedicine ; 58: 102749, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38719107

RESUMEN

New adjuvant strategies are needed to improve protein-based subunit vaccine immunogenicity. We examined the potential to use nanostructure of 6-O-ascorbyl palmitate to formulate ovalbumin (OVA) protein and an oligodeoxynucleotide (CpG-ODN) (OCC). In mice immunized with a single dose, OCC elicited an OVA-specific immune response superior to OVA/CpG-ODN solution (OC). Rheological studies demonstrated OCC's self-assembling viscoelastic properties. Biodistribution studies indicated that OCC prolonged OVA and CpG-ODN retention at injection site and lymph nodes, reducing systemic spread. Flow-cytometry assays demonstrated that OCC promoted OVA and CpG-ODN co-uptake by Ly6ChiCD11bhiCD11c+ monocytes. OCC and OC induced early IFN-γ in lymph nodes, but OCC led to higher concentration. Conversely, mice immunized with OC showed higher serum IFN-γ concentration compared to those immunized with OCC. In mice immunized with OCC, NK1.1+ cells were the IFN-γ major producers, and IFN-γ was essential for OVA-specific IgG2c switching. These findings illustrate how this nanostructure improves vaccine's response.


Asunto(s)
Nanoestructuras , Oligodesoxirribonucleótidos , Ovalbúmina , Vacunas de Subunidad , Animales , Nanoestructuras/química , Vacunas de Subunidad/inmunología , Vacunas de Subunidad/química , Vacunas de Subunidad/farmacocinética , Ratones , Oligodesoxirribonucleótidos/química , Oligodesoxirribonucleótidos/farmacocinética , Ovalbúmina/inmunología , Ovalbúmina/química , Femenino , Ratones Endogámicos C57BL , Adyuvantes Inmunológicos/química , Adyuvantes Inmunológicos/farmacocinética , Interferón gamma/metabolismo , Distribución Tisular , Ácido Ascórbico/análogos & derivados
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