The cell wall regulates dynamics and size of plasma-membrane nanodomains in Arabidopsis.

Plant plasma-membrane (PM) proteins are involved in several vital processes, such as detection of pathogens, solute transport, and cellular signaling. For these proteins to function effectively there needs to be structure within the PM allowing, for example, proteins in the same signaling cascade to be spatially organized. Here we demonstrate that several proteins with divergent functions are located in clusters of differing size in the membrane using subdiffraction-limited Airyscan confocal microscopy. Single particle tracking reveals that these proteins move at different rates within the membrane. Actin and microtubule cytoskeletons appear to significantly regulate the mobility of one of these proteins (the pathogen receptor FLS2) and we further demonstrate that the cell wall is critical for the regulation of cluster size by quantifying single particle dynamics of proteins with key roles in morphogenesis (PIN3) and pathogen perception (FLS2). We propose a model in which the cell wall and cytoskeleton are pivotal for regulation of protein cluster size and dynamics, thereby contributing to the formation and functionality of membrane nanodomains.

Movement and remodeling of the endoplasmic reticulum in nondividing cells of tobacco leaves.

Using a novel analytical tool, this study investigates the relative roles of actin, microtubules, myosin, and Golgi bodies on form and movement of the endoplasmic reticulum (ER) in tobacco (Nicotiana tabacum) leaf epidermal cells. Expression of a subset of truncated class XI myosins, which interfere with the activity of native class XI myosins, and drug-induced actin depolymerization produce a more persistent network of ER tubules and larger persistent cisternae. The treatments differentially affect two persistent size classes of cortical ER cisternae, those >0.3 microm(2) and those smaller, called punctae. The punctae are not Golgi, and ER remodeling occurs in the absence of Golgi bodies. The treatments diminish the mobile fraction of ER membrane proteins but not the diffusive flow of mobile membrane proteins. The results support a model whereby ER network remodeling is coupled to the directionality but not the magnitude of membrane surface flow, and the punctae are network nodes that act as foci of actin polymerization, regulating network remodeling through exploratory tubule growth and myosin-mediated shrinkage.

Anxiety and depression after acute myocardial infarction.

OBJECTIVES: To assess the following: (1) symptoms of anxiety and depression in hospitalized patients who had acute myocardial infarction (AMI); (2) the association between sex, infarct severity, history of previous AMI, and symptoms of anxiety and depression in hospitalized patients; (3) symptoms of anxiety and depression during the first year after AMI in a select group of patients; and (4) the association between educational and occupational status and symptoms of anxiety and depression at the time of hospitalization in a select group of patients. DESIGN: Cross-sectional survey (objectives 1 and 2) and prospective trial with random assignment (objectives 3 and 4). SETTING: Six university-affiliated hospitals in a Canadian city. PATIENTS: Seven hundred eighty-five hospitalized patients with AMI, with 1-year follow-up of 201 selected patients from this sample. INSTRUMENTS: State-Trait Anxiety Inventory and the short form of the Beck Depression Inventory. INTERVENTION: Data collection was initiated in the hospital 3 days after AMI, and patients were followed-up 14, 24, 41, and 56 weeks after AMI. RESULTS: Student t tests, analysis of variance, and descriptive statistics were used. When the patients were in the hospital the mean state (S-anxiety) and trait (T-anxiety) scores were 43 and 44, respectively. Ten percent had S-anxiety scores and 14% had T-anxiety scores that were higher than the mean scores reported for psychiatric patients. During the 1-year follow-up in the select group of subjects, the mean S-anxiety and T-anxiety scores were both 48 in-hospital, and decreased to 42 and 46, respectively, by 14 weeks and remained at these levels for the remainder of the year. During the course of the year, 16% of the patients had State-Trait Anxiety Inventory scores consistent with psychiatric conditions. The in-hospital Beck Depression Inventory mean score was 3, and 9% of the patients had scores consistent with moderate to severe depression. During the first 24 weeks, symptoms of moderate to severe depression were reported by 10% of the select group of patients. No associations were found between anxiety and depression and sex, creatine phosphokinase level, previous AMI, education, or occupational status. CONCLUSIONS: Symptoms of anxiety were prevalent among hospitalized patients who had an AMI, whereas depressive symptoms were rare. There was no association between anxiety and depression and sex, infarct severity, history of previous AMI, or educational or occupational status.

Effects on quality of life with comprehensive rehabilitation after acute myocardial infarction.

This investigation was designed to determine the impact of a brief period of cardiac rehabilitation, initiated within 6 weeks of acute myocardial infarction (AMI), on both disease-specific and generic health-related quality of life, exercise tolerance and return to work after AMI. With a stratified, parallel group design, 201 low-risk patients with evidence of depression or anxiety, or both, after AMI, were randomized to either an 8-week program of exercise conditioning and behavioral counseling or to conventional care. Although the differences were small, significantly greater improvement was seen in rehabilitation group patients at 8 weeks in the emotions dimension of a new disease-specific, health-related Quality of Life Questionnaire, in their state of anxiety and in exercise tolerance. All measures of health-related quality of life in both groups improved significantly over the 12-month follow-up period. However, the 95% confidence intervals around differences between groups at the 12-month follow-up effectively excluded sustained, clinically important benefits of rehabilitation in disease-specific (limitations, -2.70, 1.40; emotions, -4.86, 1.10, where negative values favor conventional care and positive values favor rehabilitation) and generic health-related quality of life (time trade-off, -0.062, 0.052; quality of well-being, -0.042, 0.035) or in exercise tolerance (-38.5, 52.1 kpm/min); also, return to work was similar in the 2 groups (relative risk, 0.93; confidence interval, 0.71, 1.64).(ABSTRACT TRUNCATED AT 250 WORDS)

Impediments to the practice of patient teaching.

Nurses value the practice of patient teaching and it is a stated goal of nursing. However, the actual practice of patient teaching often falls below standard. This paper describes survey data collected from staff nurses, and nursing administrators on their beliefs about patient teaching and their perceptions of the impediments to practice in the hospital setting. The findings help to clarify the conflict between the values and the practice of patient teaching. Implications for nursing practice, and the discipline of nursing are identified.

Whatsoever things are true: ways, means and values in modern psychiatry.

Epistemology and ethics are fundamental disciplines to understanding the nature of the medical enterprise. Values of truth, goodness, faith and love dominate the knowledge and practise of medicine. Each epistemological model of truth (truth as useful, truth as correspondence to fact, and truth as coherence) has strengths and weaknesses in guiding us in the scientific method in medicine. Dialectic skills are also important epistemological tools in exploring truth in relationship to diagnosis and treatment. By dialectic and analogy we approach the good as we explore and debate available technique and desirable goal. All of the doctor's scientific discipline and technical skill must be brought to bear within a relationship. It is here that the medical enterprise becomes intensely concerned with ethics. Most ethicists describe the doctor-patient relationship as a fiduciary relationship in which the doctor commits himself to his patient in faithfulness. The doctor-patient relationship is a special form of the "I-Thou relationship" grounded not only in trust but also in love.

Problem behaviours encountered by families living with a schizophrenic member.

Attention is focused on the problem behaviours encountered by relatives of schizophrenic patients. Seventy families, members of the "Friends of Schizophrenics" association completed a questionnaire aimed at eliciting specific problem behaviours from the perspective of the relative. By far, the most frequent problems were concerned with the 'negative' symptoms of schizophrenia while the problems found most difficult to manage included those to do with insight compliance and 'positive' symptoms.

The mystic experience: a psychiatric reflection.

This paper reviews the phenomenology of the mystical experience and its varied contexts epilepsy, toxicity, organic brain syndromes, the major psychoses and hysterical dissociative states as well as in apparently normal persons. The impact of the experience on the personality is noted and its significance briefly reviewed. The author notes that two fallacies await the unwary psychiatrist: the fallacy of reductionism which defines the mystical experience in pathological terms only; and the fallacy of speculation without adequate philosophical or theological tools.