Global and local characterization explains the different mechanisms of failure of the human ribs.

Knowledge of the mechanics and mechanistic reasons inducing rib fracture is fundamental for forensic investigations and for the design of implants and cardiopulmonary resuscitation devices. A mechanical rationale to explain the different rib mechanisms of failure is still a challenge. The aim of this work was to experimentally characterize human ribs to test the hypothesis that a correlation exists between the ribs properties and the mechanism of failure. 89 ribs were tested in antero-posterior compression. The full-field strain distribution was measured through Digital Image Correlation. The fracture load ranged 7-132 N. Two main different mechanisms of failure were observed: brittle and buckling. The strain analysis showed that the direction of principal strains was either aligned with the ribs, or oblique, around 45°, with a rather uniform direction in the most strained area. The maximum principal strains were in the range between 1000 and 30000 microstrain and the minimum principal strain between -30000 and -800 microstrain. The ribs undergoing brittle fracture had significantly thicker cortical bone than those undergoing buckling. Also, larger tensile strains were observed in the specimens with brittle fracture than in the buckling ones. These findings support the focus of cortical thickness modelling which could help in sharpening computational models for the aforesaid purposes.

Load-sharing biomechanics of lumbar fixation and fusion with pedicle subtraction osteotomy.

Pedicle subtraction osteotomy (PSO) is an invasive surgical technique allowing the restoration of a well-balanced sagittal profile, however, the risks of pseudarthrosis and instrumentation breakage are still high. Literature studied primary stability and posterior instrumentation loads, neglecting the load shared by the anterior column, which is fundamental to promote fusion early after surgery. The study aimed at quantifying the load-sharing occurring after PSO procedure across the ventral spinal structures and the posterior instrumentation, as affected by simple bilateral fixation alone, with interbody cages adjacent to PSO level and supplementary accessory rods. Lumbar spine segments were loaded in vitro under flexion-extension, lateral bending, and torsion using an established spine tester. Digital image correlation (DIC) and strain-gauge (SG) analyses measured, respectively, the full-field strain distribution on the ventral surface of the spine and the local strain on posterior primary rods. Ventral strains considerably decreased following PSO and instrumentation, confirming the effectiveness of posterior load-sharing. Supplemental accessory rods considerably reduced the posterior rod strains only with interbody cages, but the ventral strains were unaffected: this indicates that the load transfer across the osteotomy could be promoted, thus explaining the higher fusion rate with decreased rod fracture risk reported in clinical literature.

Reconstruction of proximal humeral fractures without screws using a reinforced bone substitute.

Multi-fragment fractures are still a challenge: current clinical practice relies on plates and screws. Treatment of fractures of the proximal humerus has the intra-operative risk of articular damage when inserting multiple screws. Distal-varus collapse of the head is a frequent complication in osteoporotic patients. The aim of this biomechanical study was to investigate if an Innovative-cement-technique (the screws are replaced by injection of cement) provides the same or better stability of the reconstructed head compared to the Standard-technique (locking screws). A four-fragment fracture was simulated in twelve pairs of humeri, with removal of part of the cancellous bone to simulate osteoporotic "eggshell" defect. One humerus of each pair was repaired either with a Standard-technique (locking plate, 2 cortical and 6 locking screws), or with the Innovative-cement-technique (injection of a partially-resorbable reinforced bone substitute consisting of PMMA additivated with 26% beta-TCP). Cement injection was performed both in the lab and under fluoroscopic monitoring. The reconstructed specimens were tested to failure with a cyclic force of increasing amplitude. The Innovative-cement-technique withstood a force 3.57 times larger than the contralateral Standard reconstructions before failure started. The maximum force before final collapse for the Innovative-cement-technique was 3.56 times larger than the contralateral Standard-technique. These differences were statistically significant. The Innovative-cement-technique, based on the reinforced bone substitute, demonstrated better biomechanical properties compared to the Standard-technique. These findings, along with the advantage of avoiding the possible complications associated with the locking screws, may help safer and more effective treatment in case of osteoporotic multi-fragment humeral fractures.

Digital Image Correlation (DIC) Assessment of the Non-Linear Response of the Anterior Longitudinal Ligament of the Spine during Flexion and Extension.

While the non-linear behavior of spine segments has been extensively investigated in the past, the behavior of the Anterior Longitudinal Ligament (ALL) and its contribution during flexion and extension has never been studied considering the spine as a whole. The aims of the present study were to exploit Digital Image Correlation (DIC) to: (I) characterize the strain distribution on the ALL during flexion-extension, (II) compare the strain on specific regions of interest (ROI) of the ALL in front of the vertebra and of the intervertebral disc, (III) analyze the non-linear relationship between the surface strain and the imposed rotation and the resultant moment. Three specimens consisting of 6 functional spinal units (FSUs) were tested in flexion-extension. The full-field strain maps were measured on the surface of the ALL, and the most strained areas were investigated in detail. The DIC-measured strains showed different values of peak strain in correspondence with the vertebra and the disc but the average over the ROIs was of the same order of magnitude. The strain-moment curves showed a non-linear response like the moment-angle curves: in flexion the slope of the strain-moment curve was greater than in extension and with a more abrupt change of slope. To the authors' knowledge, this is the first study addressing, by means of a full-field strain measurement, the non-linear contribution of the ALL to spine biomechanics. This study was limited to only three specimens; hence the results must be taken with caution. This information could be used in the future to build more realistic numerical models of the spine.

The strain distribution in the lumbar anterior longitudinal ligament is affected by the loading condition and bony features: An in vitro full-field analysis.

The role of the ligaments is fundamental in determining the spine biomechanics in physiological and pathological conditions. The anterior longitudinal ligament (ALL) is fundamental in constraining motions especially in the sagittal plane. The ALL also confines the intervertebral discs, preventing herniation. The specific contribution of the ALL has indirectly been investigated in the past as a part of whole spine segments where the structural flexibility was measured. The mechanical properties of isolated ALL have been measured as well. The strain distribution in the ALL has never been measured under pseudo-physiological conditions, as part of multi-vertebra spine segments. This would help elucidate the biomechanical function of the ALL. The aim of this study was to investigate in depth the biomechanical function of the ALL in front of the lumbar vertebrae and of the intervertebral disc. Five lumbar cadaveric spine specimens were subjected to different loading scenarios (flexion-extension, lateral bending, axial torsion) using a state-of-the-art spine tester. The full-field strain distribution on the anterior surface was measured using digital image correlation (DIC) adapted and validated for application to spine segments. The measured strain maps were highly inhomogeneous: the ALL was generally more strained in front of the discs than in front of the vertebrae, with some locally higher strains both imputable to ligament fibers and related to local bony defects. The strain distributions were significantly different among the loading configurations, but also between opposite directions of loading (flexion vs. extension, right vs. left lateral bending, clockwise vs. counterclockwise torsion). This study allowed for the first time to assess the biomechanical behaviour of the anterior longitudinal ligament for the different loading of the spine. We were able to identify both the average trends, and the local effects related to osteophytes, a key feature indicative of spine degeneration.

In Vitro Experimental Studies and Numerical Modeling to Investigate the Biomechanical Effects of Surgical Interventions on the Spine.

This paper offers a comprehensive systematic review of biomechanical research on the spine and on in vitro and numerical methods of investigation. This review focuses on interventions on the ligaments, on the facets, and on the lamina (facetectomies, laminectomies, and laminoplasties). Surgical interventions on the facets and lamina in some cases yield dissatisfactory clinical follow-up. Patient outcome is strongly related to the effects that such interventions have on the biomechanical functionality of the spine. The papers examined include those addressing the untreated spine (range of motion and stiffness), but the focus is on experimental and numerical investigations studying the role of the ligaments and of the posterior structures (including their role in granting spine stability and the biomechanical behavior of each ligament). The papers were classified based on the different investigation approaches. In vitro experiments exploit dedicated biomechanical spine testers to measure the mechanical properties of physical specimens. Numerical modeling (multibody dynamics, finite-element analysis) allows predicting the effect of different conditions. All the papers indicate that interventions on the ligaments, facets, and lamina increase range of motion and decrease stability. The quantitative results show great variability across studies. This review shows how it is possible to use in vitro and numerical methods to investigate the biomechanical effects of surgical interventions.

Full-field strain distribution in multi-vertebra spine segments: An in vitro application of digital image correlation.

The biomechanics of the spine is experimentally assessed in terms of range of motion and overall stiffness. Quantification of the surface strain distribution is currently limited either to the vertebrae or the discs, whereas a full-field approach to measure the strain distribution in a multi-vertebra segment is currently missing. The aim of this work was to explore the feasibility of using Digital Image Correlation (DIC) to measure the strain distribution simultaneously on the vertebral bodies and the intervertebral discs of spine segments in different loading configurations. Three porcine spine segments were tested. A white-on-black speckle pattern was prepared which covered the hard and soft tissues. Two different loading configurations (flexion and lateral bending) were reproduced, while two sides of the spine were analyzed with DIC. Measurements were successfully performed on the entire region of interest of all specimens, in both configurations. The DIC analysis highlighted the strain gradients present on the spine segments including tension and compression associated with bending, the direction of principal strains in the different regions, as well as bulging of the discs under compression. Strains of tens of thousands microstrain were measured in the discs, and below 2000 microstrain in the bone. This work showed the feasibility of applying DIC on spine segments including hard and soft tissues. It also highlights the need for a full-field investigation, because of the strain inhomogeneity in the vertebrae and discs.

Full-field in vitro investigation of hard and soft tissue strain in the spine by means of Digital Image Correlation.

INTRODUCTION: The spine deserves careful biomechanical investigation, because of the different types of degeneration deriving from daily stress, trauma, and hard and soft tissue pathologies. Many biomechanical studies evaluated the range of motion, structural stiffness of spine segments under different loading conditions, without addressing the strain distribution. Strain gauges have been used to measure strain in the vertebral body, in a pointwise way.What is currently missing is a method to measure the distribution of strain in the soft tissues (intervertebral discs and ligaments), and an integration between measurements in the hard and soft tissues. Digital Image Correlation (DIC) is a recently developed optical technique, which allows measuring the distribution of displacements and deformation in a contact-less way. It can provide a full-field view of the examined surface under load. DIC can therefore give a more complete knowledge of the biomechanics of the spine. METHODS: This study was performed multisegmental porcine spine specimens with two loading configurations (flexion and lateral bending), while DIC was used to measure the strain distribution. The tests showed the different deformation in the vertebral body, intervertebral discs and ligaments in compression and tension. At the same time it was possible to visualize the growth plates, which are Conclusion: Significantly softer than the vertebral bone.This work showed the feasibility of investigating the spine in a full-field way, and to quantify the strain inhomogeneity in the vertebrae and soft tissues. Therefore DIC can help improve implantable devices and the surgical technique.