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The aim of the small-scale regional engineering geological study of the Czech Republic was to evaluate the relationship between slope gradient and engineering geological zones. The research motivation was to determine the average slope gradient, 25%, 50% (median) and 75% quantiles related to the different engineering geological zones. This scientific information is critical from the perspectives of engineering geology, geotechnical engineering, and foundation engineering because an increasing slope gradient evokes the need to create a cut respectively foundation excavation or another excavation in the geological structure, which increases the probability of occurrence of the problem in terms of differential settlement and bearing capacity of the structures. The research was carried out in the territory of the Czech Republic in 8 Quaternary zones with soil foundation ground and 10 pre-Quaternary zones with rocks and semi-rocks and their eluvia. A significant difference in the statistical characteristics of slope gradients was found in the group of Quaternary engineering geological zones (evaluated group I) compared to the group of pre-Quaternary zones (evaluated group II). The value range of the average slope gradient was 1.65° (16.9%) to 5.89° (60.3%) for the Quaternary engineering geological zones (soil foundation ground), representing 43.4% difference. Whereas for the over-quaternary engineering geological zones (rocks, semi-rocks and their eluvia), the difference was much higher, 3.59° (36.8%) to 9.76° (100%-value determined as a referential because it was the maximum), which is also reflected in a more significant percentage difference of 63.2%.
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Integrative taxonomy of Diamesus Hope, 1840 (Coleoptera: Silphinae) is presented. Adults of D. bimaculatus Portevin, 1914 (endemic to Taiwan) and D. osculans (Vigors, 1825) (widely distributed from northern India to Australia) are redescribed, keyed and figured, including characters of the male and female genitalia of both species. Variation in elytral maculation in D. osculans is discussed and illustrated. The absence of diagnostic differences of D. osculans var. reductus Pic, 1917 from D. osculans is discussed, and the former name is confirmed as a junior subjective synonym of D. osculans. Types of all three names available were studied; a lectotype and paralectotypes are designated for the name D. osculans var. bimaculatus Portevin, 1914. Molecular phylogenetic analysis confirms the genus Diamesus is sister group to the genus Necrodes Leach, 1815, and D. osculans and D. bimaculatus are two, well supported clades. Detailed data on the distribution of D. bimaculatus and D. osculans are presented and mapped. Species distribution models for both species were created and interpreted. Diamesus osculans is reported for the first time from India: Uttarakhand, China: Anhui, Hainan, Hunan, Jiangxi, Shaanxi and Zhejiang Provinces, and Australia: Victoria; it is also recently confirmed from Taiwan, being sympatric in distribution there with D. bimaculatus. Available data on the ecology and seasonality of both species of Diamesus are also discussed.
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Escarabajos , Animales , Masculino , Femenino , Escarabajos/genética , Filogenia , Distribución Animal , China , Victoria , Estructuras AnimalesRESUMEN
In this article, research on the sorption properties of clay materials in comparison with commonly used building materials is published. The topic is mainly focused on the dynamic sorption properties and their influence on the relative humidity in the indoor environment. The results of comparisons of clay structures, rammed earth panels, clay plaster, and unburned bricks, with commonly used building materials, concrete, lime plaster, and gypsum board are examined. Statistically evaluated results in the form of confidence intervals are presented and the rate of dynamic sorption is analyzed. It is clear from the results that clay materials have a positive effect on the rapid adsorption and desorption of air moisture in the interior of buildings. However, there are many variables, band not every clay material has such excellent sorption properties.
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The study of bacterial degradation of 1-octylpyrrolidin-2-one (NOP) by river water and soil bacteria was the main aim of the research. Although the compound demonstrated bacteriostatic as well as bactericidal effects against Gram-positive and certain Gram-negative bacteria at concentrations ranging from 100 to 1000 mg L-1, its concentration of 100 mg L-1 was successfully degraded by microbial communities of both river water and alluvial soil; removal efficiencies reached 87.2 and 88.4% of dissolved organic carbon, respectively. Isolation of the strains responsible for the process showed that bacterial degradation was initiated by the octane-utilising bacteria of the genus Phenylobacterium, which used four carbon atoms of the NOP octyl chain and oxidised terminal carbon atom of the remaining chain. The structure of the intermediate produced by phenylobacteria was elucidated following the results obtained from the detailed electrospray mass spectrometry (ESI-MS) analysis; these experiments showed that it is a 4-(2-oxopyrrolidin-1-yl)butanoic acid. This intermediate was further degraded by other bacterial members of appropriate microbial communities, namely Bordetella petrii and Arthrobacter sp. Further tests proved that these bacteria were able to assimilate the nitrogen atom of the lactam ring and thus complete the degradation process.
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Arthrobacter , Suelo , Antibacterianos/metabolismo , Antibacterianos/farmacología , Arthrobacter/metabolismo , Biodegradación Ambiental , Carbono/metabolismo , Ríos/química , Suelo/química , Microbiología del Suelo , Agua/metabolismoRESUMEN
Providing clear and detailed morphological descriptions of endemic species in limited areas enables new knowledge of their biology and ecology to be obtained through citizen science. This information can be further used for their protection. Our study presents the first morphological description of the larvae of all three instars of Heterotemna tenuicornis (Brullé, 1836), an endemic species of the Canary Islands that, together with H. britoi García & Pérez, 1996 and H. figurata (Brullé, 1839), belongs to the peculiar genus Heterotemna Wollaston, 1864. Furthermore, we present the first sequences of two mitochondrial genes (COI, 16S) obtained from larval specimens, and cross reference them with sequences from an adult specimen. Phylogenetic analysis of molecular data placed the genus Heterotemna within the genus Silpha Linnaeus, 1758, suggesting paraphyly of Silpha. In our study, we underline the importance of using a combination of morphological description and molecular data, that can be used for barcoding developmental stages which could not otherwise be definitely associated.
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Escarabajos/anatomía & histología , Escarabajos/clasificación , Filogenia , Animales , Teorema de Bayes , Escarabajos/genética , Escarabajos/ultraestructura , Larva/anatomía & histología , Larva/clasificación , Larva/genética , Larva/ultraestructura , Especificidad de la EspecieRESUMEN
Higher selenium status has been shown to improve the clinical outcome of infections caused by a range of evolutionally diverse viruses, including SARS-CoV-2. However, the impact of SARS-CoV-2 on host-cell selenoproteins remains elusive. The present study investigated the influence of SARS-CoV-2 on expression of selenoprotein mRNAs in Vero cells. SARS-CoV-2 triggered an inflammatory response as evidenced by increased IL-6 expression. Of the 25 selenoproteins, SARS-CoV-2 significantly suppressed mRNA expression of ferroptosis-associated GPX4, DNA synthesis-related TXNRD3 and endoplasmic reticulum-resident SELENOF, SELENOK, SELENOM and SELENOS. Computational analysis has predicted an antisense interaction between SARS-CoV-2 and TXNRD3 mRNA, which is translated with high efficiency in the lung. Here, we confirmed the predicted SARS-CoV-2/TXNRD3 antisense interaction in vitro using DNA oligonucleotides, providing a plausible mechanism for the observed mRNA knockdown. Inhibition of TXNRD decreases DNA synthesis which is thereby likely to increase the ribonucleotide pool for RNA synthesis and, accordingly, RNA virus production. The present findings provide evidence for a direct inhibitory effect of SARS-CoV-2 replication on the expression of a specific set of selenoprotein mRNAs, which merits further investigation in the light of established evidence for correlations between dietary selenium status and the outcome of SARS-CoV-2 infection.
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ADN/biosíntesis , Estrés del Retículo Endoplásmico/fisiología , Ferroptosis/fisiología , ARN Mensajero/metabolismo , SARS-CoV-2/fisiología , Selenoproteínas/metabolismo , Animales , Chlorocebus aethiops , Regulación de la Expresión Génica/fisiología , ARN Mensajero/genética , Selenoproteínas/genética , Células VeroRESUMEN
The rapid and reliable recognition of nucleic acid sequences is essential to a broad range of fields including genotyping, gene expression analysis, and pathogen screening. For viral detection in particular, the capability is critical for optimal therapeutic response and preventing disease transmission. Here, we report an approach for detecting identifying sequence motifs within genome-scale single-strand DNA and RNA based on solid-state nanopores. By designing DNA oligonucleotide probes with complementarity to target sequences within a target genome, we establish a protocol to yield affinity-tagged duplex molecules the same length as the probe only if the target is present. The product can subsequently be bound to a protein chaperone and analyzed quantitatively with a selective solid-state nanopore assay. We first use a model DNA genome (M13mp18) to validate the approach, showing the successful isolation and detection of multiple target sequences simultaneously. We then demonstrate the protocol for the detection of RNA viruses by identifying and targeting a highly conserved sequence within human immunodeficiency virus (HIV-1B).
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Nanoporos , Ácidos Nucleicos , Secuencia Conservada , ADN , Sondas de ADN , HumanosRESUMEN
A new species, Oiceoptoma tangi Sommer, Ruzicka, Nishikawa Schneider, new species, from Zhejiang Province, China, is described and illustrated. The new species is closely related to O. subrufum (Lewis, 1888), distributed in Central and North-eastern China, the Korean Peninsula, Far East of Russia and Japan, and to O. nigropunctatum (Lewis, 1888), endemic to Japan. All three species are keyed, and distribution maps of all three species are presented.
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Escarabajos , Distribución Animal , Animales , China , Escarabajos/clasificación , Escarabajos/fisiologíaRESUMEN
The HIV-1 nef gene terminates in a 3'-UGA stop codon, which is highly conserved in the main group of HIV-1 subtypes, along with a downstream potential coding region that could extend the nef protein by 33 amino acids, if readthrough of the stop codon occurs. Antisense tethering interactions (ATIs) between a viral mRNA and a host selenoprotein mRNA are a potential viral strategy for the capture of a host selenocysteine insertion sequence (SECIS) element (Taylor et al, 2016) [1]. This mRNA hijacking mechanism could enable the expression of virally encoded selenoprotein modules, via translation of in-frame UGA stop codons as selenocysteine (SeC). Here we show that readthrough of the 3'-terminal UGA codon of nef occurs during translation of HIV-1 nef expression constructs in transfected cells. This was accomplished via fluorescence microscopy image analysis and flow cytometry of HEK 293 cells, transfected with engineered GFP reporter gene plasmid constructs, in which GFP can only be expressed by translational recoding of the UGA codon. SiRNA knockdown of thioredoxin reductase 1 (TR1) mRNA resulted in a 67% decrease in GFP expression, presumably due to reduced availability of the components involved in selenocysteine incorporation for the stop codon readthrough, thus supporting the proposed ATI. Addition of 20 nM sodium selenite to the media significantly enhanced stop codon readthrough in the pNefATI1 plasmid construct, by >100%, supporting the hypothesis that selenium is involved in the UGA readthrough mechanism.
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Selenium status plays a major role in health impacts of various RNA viruses. We previously reported potential antisense interactions between viral mRNAs and host mRNAs encoding isoforms of the antioxidant selenoprotein thioredoxin reductase (TXNRD). Here, we examine possible targeting of selenoprotein mRNAs by Zika virus (ZIKV), because one of the most devastating outcomes of ZIKV infection in neonates, microcephaly, is a key manifestation of Progressive Cerebello-Cerebral Atrophy (PCCA), a genetic disease of impaired selenoprotein synthesis. Potential antisense matches between ZIKV and human selenoprotein mRNAs were identified computationally, the strongest being against human TXNRD1 and selenoprotein P (SELENOP), a selenium carrier protein essential for delivery of selenium to the brain. Computationally, ZIKV has regions of extensive (~30bp) and stable (ΔE < -50kcal/mol) antisense interactions with both TXNRD1 and SELENOP mRNAs. The core ZIKV/SELENOP hybridization was experimentally confirmed at the DNA level by gel shift assay using synthetic oligonucleotides. In HEK293T cells, using Western blot probes for SELENOP and TXNRD1, ZIKV infection knocked down SELENOP protein expression almost completely, by 99% (p<0.005), and TXNRD1 by ~90% (p<0.05). In contrast, by RT-qPCR, there was no evidence of significant changes in SELENOP and TXNRD1 mRNA levels after ZIKV infection, suggesting that their knockdown at the protein level is not primarily a result of mRNA degradation. These results suggest that knockdown of SELENOP and TXNRD1 by ZIKV in fetal brain, possibly antisense-mediated, could mimic SELENOP knockout, thereby contributing to neuronal cell death and symptoms similar to the genetic disease PCCA, including brain atrophy and microcephaly.
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The postmortem interval (PMI) estimation, in cases where the body was discovered in an advanced stage of decomposition, is predominantly based on entomological evidence. However, very few forensically important species are sufficiently known in detail to allow a practical application. One of them is the carrion beetle, Necrophila (Calosilpha) brunnicollis (Kraatz, 1877). Its development from egg to adulthood was studied under a range of ecologically relevant constant temperatures to find parameters of thermal summation models. Developmental sexual dimorphism and the presence of developmental rate isomorphy were investigated. Herein we present the lower developmental thresholds and sum of effective temperatures for all developmental stages of N. brunnicollis (egg, first-third larval instar, postfeeding stage, and pupae). We did not find any evidence of developmental sexual dimorphism nor was the presence of developmental rate isomorphy confirmed. Our results present the first thermal summation model of the East Asian carrion beetle that can be used for the PMI estimation.
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Escarabajos/crecimiento & desarrollo , Entomología Forense/métodos , Animales , Cadáver , Larva/crecimiento & desarrollo , Rasgos de la Historia de Vida , Modelos Teóricos , Cambios Post Mortem , Pupa/crecimiento & desarrollo , Factores Sexuales , TemperaturaRESUMEN
Carrion beetles of genus Thanatophilus (Leach, 1815) are an important group of necrophagous insects, with great potential for forensic entomology in temperate zones of Africa, America, Asia, and Europe. Developmental models for majority of Thanatophilus species remain unknown. In this study, we will provide new thermal summation models for all the developmental stages of Thanatophilus sinuatus (Fabricius, 1775), one of the most abundant and widespread species of the genus. The beetles were bred at seven different constant temperatures, and developmental time was measured for each developmental stage (egg, three larval instars, postfeeding stage, and pupa). Temperature-sex influence was tested, and thermal summation constants were calculated to be used for postmortem interval estimation during criminal investigations.
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Escarabajos/crecimiento & desarrollo , Entomología Forense , Animales , Larva/crecimiento & desarrollo , Modelos Biológicos , Óvulo/crecimiento & desarrollo , Pupa/crecimiento & desarrolloRESUMEN
An environmental isolate Comamonas testosteroni RF2 has been previously described to cometabolize trichloroethene (TCE), 1,2-cis-dichloroethene (cDCE), 1,2-trans-dichloroethene (tDCE), and 1,1-dichloroethene (1,1DCE) when grown on phenol and lactate sodium. In this study, three vinyl chloride (VC) degrading strains, Mycobacterium aurum L1, Pseudomonas putida PS, and Rhodococcus ruber Sm-1 were used to form consortia with the strain RF2 in terms to achieve the removal of VC along with above-mentioned chloroethenes. Degradation assays were performed for a binary mixture of cDCE and VC as well as for a mixture of TCE, all DCEs and VC. The consortium composed of C. testosteroni RF2 and M. aurum L1 showed to be the most efficient towards the removal of cDCE (6.01 mg L-1) in the binary mixture with VC (10 mg L-1) and was capable of efficiently removing chloroethenes in the mixture sample at the initial concentrations of 116 µg L-1 for TCE, 662 µg L-1 for cDCE, 42 µg L-1 for tDCE, 16 µg L-1 for 1,1DCE, and 7 mg L-1 for VC with a removal efficiency of nearly 100% for all of the compounds. Although complete removal of VC took a significantly longer time than the removal of other chloroethenes, the consortium composed of C. testosteroni RF2 and M. aurum L1 displayed strong bioremediation potential for aquifers with downstream contamination characterized by the presence of less chlorinated ethenes.
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Comamonas testosteroni , Tricloroetileno , Cloruro de Vinilo , Biodegradación Ambiental , Dicloroetilenos , Mycobacteriaceae , RhodococcusAsunto(s)
Proteasas Similares a la Papaína de Coronavirus/metabolismo , Proteína S/metabolismo , SARS-CoV-2/enzimología , Coagulación Sanguínea , Dominio Catalítico , Proteasas Similares a la Papaína de Coronavirus/química , Humanos , Conformación Proteica , Proteolisis , Relación Estructura-Actividad , Especificidad por SustratoRESUMEN
Larval morphology of all three instars of Nicrophorus (Nicrophorus) nepalensis Hope, 1831 (Coleoptera: Silphidae: Nicrophorinae) is described and illustrated, based on reared larvae. The eastern Palaearctic and Oriental N. (N.) nepalensis is similar to the western Palaearctic N. (N.) humator (Gleditsch, 1767) in a number of larval characters. This is congruent with recent classification of the genus Nicrophorus Fabricius, 1775 by Sikes, who suggested a close phylogenetic affinity of the N. nepalensis species group (with 16 species) with the N. humator species group (2 species). The generic description of larvae of Nicrophorus Fabricius, 1775 is expanded, based on detailed SEM observation.
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Escarabajos , Animales , Larva , FilogeniaRESUMEN
Necrophagous beetles of genus Thanatophilus are well recognized as a group of beetles with a high potential utility in forensic entomology. They can be used to estimate postmortem interval (PMI) or validate the value for other groups of insects commonly encountered on human remains, like blowflies (Calliphoridae). However, reliable tools for instar and species identification of their larvae are needed as such information is crucial for allowing accurate PMI estimate. One of the most common species of the genus Thanatophilus in Europe is Thanatophilus sinuatus. This species occurs frequently on human remains and its larvae feed on decaying tissues throughout their development. Therefore, the larvae could become useful bioindicators for forensic entomology, although their current description does not allow reliable instar or species identification. Our goal was to provide morphological characters for species and instar identification of all larval stages of T. sinuatus. The larvae were obtained from laboratory rearing under controlled conditions (20 °C and 16:8 h of light/dark period). Qualitative and quantitative morphological instar and species-specific characters are described and illustrated. Additionally, we report observations of biological and developmental lengths for all stages of the species. We also compared these morphological characters with recent description of T. rugosus and provided an identification key of these two similar and often co-occurring species. We noticed that some characters for instar identification were shared between T. sinuatus and T. rugosus and were confirmed by comparison with larvae of T. dentigerus that they can be applied to other species of the genus.
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Escarabajos/clasificación , Escarabajos/crecimiento & desarrollo , Entomología Forense , Larva/crecimiento & desarrollo , Pupa/crecimiento & desarrollo , Animales , Estudios de Evaluación como Asunto , Conducta Alimentaria , Larva/clasificación , Microscopía Electrónica de Rastreo , Imagen Óptica , Pupa/clasificaciónRESUMEN
A total of 24 species and subspecies of Silphidae (ten belonging to the subfamily Nicrophorinae and 14 to Silphinae) are reported from Turkey. This list combines a literature review with examination of specimens collected in various regions of Turkey, and mostly housed at the Metin Aktas Zoological Museum of Gazi University, Ankara, Turkey and in the collection of Jan Ruzicka, Prague, Czech Republic. One species, Silpha olivieri Bedel, 1887, is reported from Turkey for the first time. The occurrence of Nicrophorus sepultor Charpentier, 1825 in Turkey is confirmed, based on abundant recently collected material from north-western Turkey. For several other species, we provide new province records within Turkey. Thanatophilus ferrugatus (Solsky, 1874) is excluded from the Turkish fauna, since the published record was based on misidentification of the similar T. terminatus (Hummel, 1825). We provide a key to adults of species and subspecies known from Turkey, and distribution maps and photographs of all taxa.
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Escarabajos , Animales , República Checa , TurquíaRESUMEN
Ptomaphaginus troglodytessp. n., the first anophthalmic species of Ptomaphaginus Portevin, 1914 is described from two close caves in Libo Karst, south Guizhou Province, China.
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Determination of insect species and their instars, occurring on human remains, is important information that allows us to use insects for estimation of postmortem interval and detect possible manipulation with the body. However, larvae of many common species can be identified only by molecular methods, which is not always possible. The instar determination is even more challenging, and qualitative characters that would allow a more precise identification are mostly unknown. Thanatophilus rugosus (Linnaeus, 1758) is a common necrophagous beetle in the whole Palaearctic region from Europe to Japan. The species is often encountered on corpses of large vertebrates including humans, and its potential to become a useful bioindicator for forensic entomology is therefore high. Adults can be easily distinguished from other species; however, larvae were never thoroughly described to allow species and instar identification. The aim of this study was to provide reliable morphological characters that would allow species and instar identification of T. rugosus larvae. The material for morphological study was obtained from rearing under controlled conditions (20 °C and 12:12 h of light/dark period), and specimens that were not studied morphologically were allowed to complete their development. Quantitative and qualitative morphological characters for instar and species identification are described and illustrated. Additionally, we report observations of biology and developmental length for all stages of the species.
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Escarabajos/crecimiento & desarrollo , Larva/crecimiento & desarrollo , Animales , Ciencias Forenses , Microscopía Electrónica de Rastreo , Imagen ÓpticaRESUMEN
Omoglymmius (s. str.) wukongsp. n. (Coleoptera: Rhysodidae: Omoglymmiini) is described from Xizang, China. Relevant morphological characters of the new species are illustrated with colour plates, and known distribution of the subgenus Omoglymmius in the Himalayan region is mapped.