An unusual duo: Immunodeficiency disorder and scleroderma.

A 45-year-old woman on treatment for HIV infection with highly active antiretroviral therapy for the past 10 years presented to us with a history of Raynaud's phenomenon and hyperpigmentation of the skin for 2 years. She was diagnosed to have pulmonary arterial hypertension 8 months ago. On examination, she had salt-and-pepper pigmentation and sclerodactyly. Her biochemical workup was normal. She had positive antinuclear antibody by indirect immunofluorescence method. Skin biopsy was consistent with systemic sclerosis. HIV has its own musculoskeletal manifestations. The paradox of autoimmunity in the background of immunodeficiency was intriguing. Treating autoimmunity in the presence of immunodeficiency was challenging. The attribution and differentiation of pulmonary hypertension were difficult. There has been a homology identified between human immunodeficiency virus 1 (HIV 1) and centromere B protein (CENP B). This case is reported because of the unusual occurrence of systemic sclerosis in an HIV patient.

A Rare Presentation of Extrahepatic Biliary Neuroendocrine Tumor Diagnosed using 68Ga-DOTA-TOC Imaging, But Undetectable on 68Ga-FAPI Imaging.

Neuroendocrine tumors (NETs) are commonly seen in the small intestine and rarely found within the bile ducts. This low incidence is due to a smaller number of Kulchitsky cells in the extrahepatic biliary tree, which predisposes to the disease. The diagnosis of biliary tree carcinoid preoperatively is very rare, with most cases in the literature being incidentally diagnosed during surgery or being identified on the histopathology report postoperatively. Here, we present an interesting case of an extrahepatic biliary NET which was diagnosed preoperatively.

A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(II) complex with BSA and DNA complemented by docking studies.

A fluorescent molecule, pyridine-coupled bis-anthracene (PBA), has been developed for the selective fluorescence turn-on detection of Cu2+. Interestingly, the ligand PBA also exhibited a red-shifted ratiometric fluorescence response in the presence of water. Thus, a ratiometric water sensor has been utilized as a selective fluorescence turn-on sensor for Cu2+, achieving a 10-fold enhancement in the fluorescence and quantum yield at 446 nm, with a lower detection limit of 0.358 µM and a binding constant of 1.3 × 106 M-1. For practical applications, sensor PBA can be used to detect Cu2+ in various types of soils like clay soil, field soil and sand. The interaction of the PBA-Cu(II) complex with transport proteins like bovine serum albumin (BSA) and ct-DNA has been investigated through fluorescence titration experiments. Additionally, the structural optimization of PBA and the PBA-Cu(II) complex has been demonstrated by DFT, and the interaction of the PBA-Cu(II) complex with BSA and ct-DNA has been analyzed using theoretical docking studies.

Design of modified reference phase modulation based boost chopper fed fifteen level stepped DC link hybrid converter.

A new fifteen-level stepped DC to AC hybrid converter is proposed for Solar Photovoltaic (SPV) applications. A boost chopper circuit is designed and interfaced with the fifteen-level hybrid converters specific to Electric Vehicles' Brushless DC Motor (BLDC) drive systems. In chopper units, the output of solar panels is regulated and stepped up to obtain the nominal output voltage. In the stepped DC-link hybrid converter configuration, fifteen-level DC-link voltage is achieved by the series-operated DC-link modules with reduced electrical energy compression. From the comprehensive structure, it is anecdotal that the proposed topology has achieved minimum switching and power loss. Elimination of end passive components highlights the merits of the proposed hybrid systems. The reduction of controlled power semiconductor switches and gate-firing circuits has made the system more reliable than other hybrid converters. From the extensive analysis, the experimental setup has reported that 7% reduction in harmonics and a 54% reduction in controlled power switches than the existing fifteen-level converter topologies. Mitigation of power quality issues in the voltage profile of a fifteen-level multilevel hybrid converter is achieved through the implementation of dsPIC digital-controller-based gate triggering circuits.

A Rare Case of Grisel's Syndrome in A 6-Year-Old Child.

Introduction: C1-2 rotatory subluxation is more commonly seen in children compared to in adults. It often has a history of respiratory tract infection, cervical trauma, and recent history of surgery of the head or neck. Case Report: A 6-year-old boy presented to us with complaints of insidious onset of progressive deformity of the neck since the past 3 months. On examination, the patient had a classic "cock robin" deformity with his left head tilt and right-sided chin rotation. There was tenderness and spasm of the left sternocleidomastoid muscle. Radiologically, the child had unilateral C1-C2 facetal dislocation. There were associated abnormalities consisting of unilateral occiputoatlantal fusion and C2-C3 fusion. Magnetic resonance imaging (MRI) showed C1-C2 subluxation with kyphotic deformity the apex of which was impinging on the brainstem. The patient was put on skull traction with Crutchfield Tongs with progressively increasing weights for 1 week and serial X-rays were taken. Computed tomography (CT) scan was repeated at the end of 1 week which showed no improvement. C1-C2 open reduction and fusion was done. Post-operative period was uneventful. He improved on serial follow-ups. At follow-up at 18 months, the child remains comfortable, is going to school and doing all indoor and outdoor activities. His posture continues to be balanced. Radiologically, C1-C2 joint shows signs of a solid fusion. Conclusion: A thorough history taking and a meticulous clinical examination if important for evaluation of torticollis in a child. Proper imaging helps in confirming the diagnosis and grading the severity. Prompt treatment is necessary for getting a good outcome.

Viscosity-sensitive and AIE-active bimodal fluorescent probe for the selective detection of OCl- and Cu2+: a dual sensing approach via DFT and biological studies using green gram seeds.

A novel dual-mode viscosity-sensitive and AIE-active fluorescent chemosensor based on the naphthalene coupled pyrene (NCP) moiety was designed and synthesized for the selective detection of OCl- and Cu2+. In non-viscous media, NCP exhibited weak fluorescence; however, with an increase in viscosity using various proportions of glycerol, the fluorescence intensity was enhanced to 461 nm with a 6-fold increase in fluorescence quantum yields, which could be utilized for the quantitative determination of viscosity. Interestingly, NCP exhibited novel AIE characteristics in terms of size and growth in H2O-CH3CN mixtures with high water contents and different volume percentage of water, which was investigated using fluorescence, DLS study and SEM analysis. Interestingly, this probe can also be effectively employed as a dual-mode fluorescent probe for light up fluorescent detection of OCl- and Cu2+ at different emission wavelengths of 439 nm and 457 nm via chemodosimetric and chelation pathways, respectively. The fast-sensing ability of NCP towards OCl- was shown by a low detection limit of 0.546 µM and the binding affinity of NCP with Cu2+ was proved by a low detection limit of 3.97 µM and a high binding constant of 1.66 × 103 M-1. The sensing mechanism of NCP towards OCl- and Cu2+ was verified by UV-vis spectroscopy, fluorescence analysis, 1H-NMR analysis, mass spectroscopy, DFT study and Job plot analysis. For practical applications, the binding of NCP with OCl- and Cu2+ was determined using a dipstick method and a cell imaging study in a physiological medium using green gram seeds.

A Prompt Study on Recent Advances in the Development Of Colorimetric and Fluorescent Chemosensors for "Nanomolar Detection" of Biologically Important Analytes.

Fluorescent and colorimetric chemosensors for selective detection of various biologically important analytes have been widely applied in different areas such as biology, physiology, pharmacology, and environmental sciences. The research area based on fluorescent chemosensors has been in existence for about 150 years with the development of large number of fluorescent chemosensors for selective detection of cations as metal ions, anions, reactive species, neutral molecules and different gases etc. Despite the progress made in this field, several problems and challenges still exist. The most important part of sensing is limit of detection (LOD) which is the lowest concentration that can be measured (detected) with statistical significance by means of a given analytical procedure. Although there are so many reports available for detection of millimolar to micromolar range but the development of chemosensors for the detection of analytes in nanomolar range is still a challenging task. Therefore, in our current review we have focused the history and a general overview of the development in the research of fluorescent sensors for selective detection of various analytes at nanomolar level only. The basic principles involved in the design of chemosensors for specific analytes, binding mode, photophysical properties and various directions are also covered here. Summary of physiochemical properties, mechanistic view and type of different chemosensors has been demonstrated concisely in the tabular forms.

A systematic review on factors associated with non-alcoholic fatty liver disease (NAFLD) among adolescents.

BACKGROUND & AIMS: Non-Alcoholic Fatty Liver Disease (NAFLD) is a global public health risk. The occurrence of adolescent NAFLD coincides with high rates of overweight and obesity, with an unhealthy lifestyle also playing a role. Data on prevalence and factors contributing to NAFLD among Asian adolescents is lacking as most studies focus on adults. This systematic review aims to determine the prevalence and factors contributing to NAFLD among adolescents. METHODS: A systematic search was conducted using five (Goh et al., 2013) [5] databases: Cochrane, PubMed, Scopus, Science Direct, EBSCO and grey literature. Two reviewers independently screened studies using predefined inclusion and exclusion criteria and performed data extraction. Assessment of methodological quality was completed using the Newcastle-Ottawa checklist. RESULTS: The quality of most studies were of high quality, with the majority reporting no association between lifestyle factors and NAFLD. A total of 6 studies were included in this systematic review. The prevalence of NAFLD among adolescents varied between 8.0% (Fraser et al., 2007) in a study on 5586 adolescents aged 12-19 and 16.0% (Chen et al., 2009) in another survey of 1724 adolescents aged 12-13 years old. Snacking habits and lack of physical activity had potential associations with adolescent NAFLD. Current evidence shows that lifestyle factor (Western dietary pattern) is associated with a higher risk of developing NAFLD among adolescents. CONCLUSIONS: Lifestyle factors, including snacking habits and lack of physical activity, were associated with a higher risk of developing NAFLD among adolescents from high-income countries. The difference in the prevalence of NAFLD between countries with different incomes requires further investigation.

Integrated Edge Deployable Fault Diagnostic Algorithm for the Internet of Things (IoT): A Methane Sensing Application.

The Internet of Things (IoT) is seen as the most viable solution for real-time monitoring applications. But the faults occurring at the perception layer are prone to misleading the data driven system and consume higher bandwidth and power. Thus, the goal of this effort is to provide an edge deployable sensor-fault detection and identification algorithm to reduce the detection, identification, and repair time, save network bandwidth and decrease the computational stress over the Cloud. Towards this, an integrated algorithm is formulated to detect fault at source and to identify the root cause element(s), based on Random Forest (RF) and Fault Tree Analysis (FTA). The RF classifier is employed to detect the fault, while the FTA is utilized to identify the source. A Methane (CH4) sensing application is used as a case-study to test the proposed system in practice. We used data from a healthy CH4 sensing node, which was injected with different forms of faults, such as sensor module faults, processor module faults and communication module faults, to assess the proposed model's performance. The proposed integrated algorithm provides better algorithm-complexity, execution time and accuracy when compared to FTA or standalone classifiers such as RF, Support Vector Machine (SVM) or K-nearest Neighbor (KNN). Metrics such as Accuracy, True Positive Rate (TPR), Matthews Correlation Coefficient (MCC), False Negative Rate (FNR), Precision and F1-score are used to rank the proposed methodology. From the field experiment, RF produced 97.27% accuracy and outperformed both SVM and KNN. Also, the suggested integrated methodology's experimental findings demonstrated a 27.73% reduced execution time with correct fault-source and less computational resource, compared to traditional FTA-detection methodology.

Association of Serum Cystatin C Level With Carotid Arterial Wall Elastic Resistance as a Potential Marker for Detection of Early Stage Atherosclerosis.

BACKGROUND: Early diagnosis of atherosclerosis is exigent in patients with known cardiovascular disease (CVD) risk factors. During the initial phases of atherosclerosis, appearance of plaques can be detected by the ultrasonic phased tracking method which measures the arterial wall elasticity. However, reliable and easily available biochemical markers are not evaluated in the diagnosis of early-stage atherosclerosis. So the current study was carried out to assess the serum cystatin C level as an atherosclerotic marker, by evaluating its association with carotid arterial elastic modulus using the phased tracking method. MATERIALS AND METHODS: A cross-sectional study was conducted on 115 patients having risk factors for atherosclerosis but not meeting carotid intima-media thickness (IMT) criteria. The early-stage atherosclerosis was detected by using the ultrasonic phased tracking method and the patients were divided based on low and high carotid elastic modulus. Serum levels of cystatin-C were measured in association with IMT, and elastic modulus was calculated using a novel method. This study also put forth the evaluation of the sensitivity and specificity of cystatin C for early diagnosis of atherosclerosis. RESULTS: Cystatin C was strongly related to carotid elasticity (r=0.650). Based on multi-linear regression analysis, cystatin C showed significant association with carotid elasticity (ß=0.509; p<0.001). It also displayed significant positive association with high carotid elastic modulus (ß=0.511; p=0.02). Cystatin C showed a sensitivity of 85% in the prediction of high carotid elastic modulus. CONCLUSION: For patients who are at risk to evolve atherosclerosis but are not evident with arterial plaques, cystatin C exhibits a significant association with carotid wall elastic modulus, which eases the detection of atherosclerosis. Thus, cystatin C is a potential biochemical marker for early diagnosis of atherosclerosis.

A fast survey on recent developments in designing colorimetric and fluorescent sensors for the selective detection of essential amino acids.

Owing to the biological significance of various amino acids, developing accurate and cost-effective sensing techniques for the selective detection of amino acids has recently attracted growing interest. This review discusses the recent advancements of chemosensors in the selective detection of only essential amino acids out of a total of twenty amino acids, which have been applied in chemosensing research, and the mechanism of their action. The focus is directed towards the detection of the most important essential amino acids, like leucine, threonine, lysine, histidine, tryptophan and methionine, since isoleucine and valine are yet to be explored in regard to chemosensing. According to their chemical and fluorescence properties, different sensing techniques, such as the reaction-based approach, DNA-based sensors, nanoparticle formation, coordination ligand binding, host-guest chemistry, the fluorescence indicator displacement (FID) approach, electrochemical sensors, carbon dot-based sensors, MOF-based sensors and metal-based techniques, have been described.

Surgical and Survival Outcomes of Operable Gastric Cancer-Experience from a Tertiary Care Center in South India.

Gastric cancer is one of the leading cancers in Southern India. Data regarding the gastric cancers among the Indian population is sparse. Most patients in our country have locally advanced gastric cancers due to delayed presentation. In this article, we present our data regarding the presentation patterns, epidemiological demographics, surgical outcomes, and survival patterns from a tertiary care center in South India. This is a retrospective analysis of gastric cancer patients who underwent gastrectomy in our institution between January 2015 and November 2021 (n = 102). The data regarding patient characteristics, histopathology, and perioperative outcomes were analysed from medical records. The adjuvant treatment received and survival details were collected from the follow-up records and by telephonic interviews. A total of 128 patients were assessable, 102 patients underwent gastrectomy in a period of 6 years. The median age of presentation was 60 years and males were more commonly affected (70.6%). Most common presentation was pain abdomen followed by gastric outlet obstruction. Adenocarcinoma NOS (93%) was the most common histological type. Most of the Patients had antropyloric growths (79.4%) and subtotal gastrectomy with D2 lymphadenectomy was the most common surgery performed. Majority of the tumors were T4 tumors (55.9%) and nodal metastases were detected in 74% of the specimens. Predominant morbidity was wound infection (6.1%) followed by anastomotic leak (5.9%) with a combined overall morbidity of 16.7% and 30-day mortality of 2.9%. Seventy five (80.5%) patients were able to complete all planned 6 cycles of adjuvant chemotherapy. The median time of survival calculated by Kaplan-Meier method was 23 months with 2-year and 3-year overall survival rates of 31% and 22%, respectively. Lymphovascular invasion (LVSI) and lymph nodal burden were the risk factors associated with recurrences and deaths. The patient characteristics, histological factors, and perioperative outcomes revealed most of our patients presented in locally advanced stages with poor risk histological types and increased nodal burden contributing to the lower survival in our population. Inferior survival outcomes suggest the need to explore perioperative and neoadjuvant chemotherapy options in our population.

Aging resistance of infiltrated monolithic zirconia compared to noninfiltrated monolithic zirconia: A systematic review of in vitro studies.

Aim: The aim of the study is to systematically assess the impact of low-temperature degradation (LTD) simulation in an autoclave on mechanical and microstructural properties of infiltrated monolithic zirconia compared to the noninfiltrated zirconia. Settings and Design: Systematic review following Preferred Reporting Items for Systematic Reviews and Meta-Analysis 2020 guidelines. Materials and Methods: An electronic search was done within these databases: PubMed, Scopus, and Web of Science, Science Direct, Embase, Wiley, Google Scholar for articles published between 2000 and March 2021. Search results that met eligibility criteria were categorized into two groups based on properties assessed of infiltrated monolithic zirconia exposed to LTD (also called aging simulation) - (a) mechanical (flexural strength and fracture toughness) and (b) microstructural properties (phase transformation rate and m content). Statistical Analysis Used: Qualitative analysis. Results: The search identified 272 preliminary results. After discarding duplicates, and screening of titles, abstracts, and full texts, 10 articles finally met inclusion criteria. Data were collected on author's details and their countries, journal and year of publication, type and percentage of infiltration, aging protocol (duration and temperature), mechanical, and microstructural properties. All the included studies invariably revealed better aging resistance without a change in mechanical properties for infiltrated monolithic zirconia as compared to noninfiltrated species. Conclusion: Infiltration within monolithic zirconia can reduce degradation and simultaneously maintain their mechanical properties by preventing water entry into grain contours. The final m content was less for infiltrated Zirconium, indicating a lesser phase transformation and better aging resistance. Other Information: Systematic review protocol registered at PROSPERO CRD42021248153.

Observational evidence on the coastal upwelling along the northwest coast of India during summer monsoon.

The lesser known coastal upwelling in the North Eastern Arabian Sea (NEAS) during summer monsoon, its associated dynamics and forcing mechanisms is elucidated for the first time using basin scale monthly time-series in-situ and satellite data. The presence of cool upwelled waters along northwest coast of India from July to early October with an associated increase in productivity was evident in both data. The low level Findlater jet blows towards west coast of India with high wind magnitude (10-12 m/s) during summer monsoon generates strong Ekman transport (1416 kg/m/s) at offshore and Ekman pumping velocity (1.349 m/s) at coastal region initiates upwelling. It was identified that the currents and remote forcing also regulate upwelling along the region. Although upwelling seems to exist along the northwest coast, it was weaker (25.5 °C) compared to the southwest coast where the SST dropped to 24 °C. The upwelling was observed in the south during June as a surface process, while it was observed along the northwest coast of India by the end of August. Even though the onset of upwelling in the NEAS and South Eastern Arabian Sea (SEAS) had a lag of two months, the recession of upwelling happened during late and early September respectively. The cause for the lag in the onset and cessation of upwelling between SEAS and NEAS is attributed to the propagation of Kelvin waves and southwest monsoon winds. The study also reveals that temperature and chlorophyll profiles show bi-modal peaks of high and low associated with winter cooling (winter) and upwelling (summer).

Description on variability of shelf-edge hydrography and current structure of the South Eastern Arabian Sea during summer and winter monsoons.

The paper describes the hydrography and vertical current structure along the shelf edge of South East Arabian Sea (SEAS) during summer and winter monsoons based on current profiles from moving Acoustic Doppler Current Profiler (ADCP). During summer monsoon, SEAS was characterized by upwelling with low saline water at the surface along the southern sector (8° N to 11° N). During winter, thermal structure was vertically homogeneous in the upper 80 m, and intrusion of low saline Bay of Bengal waters were found up to 14° N. In the southern sector, turbidity was more than the northern sector during winter and summer seasons. ADCP-derived current profiles during summer along 200-m isobath show dominant northward flow in the south, and southeasterly in the north as part of the West India Coastal Current (WICC). A comparison between ADCP current profiles and Ekman currents during summer indicates dominance of remote forcing (coastal Kelvin waves) over the local wind forcing in the 8-9° N sector whereas a combined influence of both remote forcing and wind in the 9-15° N sector. During winter, the direction of surface current reversed and was poleward generally except at the southern sector (7-8° N) where the flow was southwestward. Sector-wise comparison of ADCP and Ekman current showed less influence of wind on current fields throughout the sector except at south; wind has a major role in the current generation, whereas along the 8-15° N sector, the remote forcing dominates over the wind.

Geomorphic response to sea level and climate changes during Late Quaternary in a humid tropical coastline: Terrain evolution model from Southwest India.

The coastal lands of southern Kerala, SW India in the vicinity of Achankovil and Thenmala Shear Zones reveal a unique set of geomorphic features like beach ridges, runnels, chain of wetlands, lakes, estuaries, etc. The chain of wetlands and water bodies that are seen in the eastern periphery of the coastal lands indicates the remnants of the upper drainage channels of the previously existed coastal plain rivers of Late Pleistocene age that are later broadened due to coastal erosion under the transgressive phase. The terrain evolutionary model developed from the results of the study shows that the Late Pleistocene transgressive events might have carved out a major portion of the land areas drained by the coastal plain rivers and as a result the coastal cliff has been retreated several kilometers landwards. The NNE-SSW trending beach ridges located close to the inland wetlands indicate the extent of shoreline shift towards eastwards during Late Pleistocene period. The present beach parallel ridges in the younger coastal plain indicate the limit of the Mid Holocene shoreline as the transgression was not so severe compared to Late Pleistocene event. The zone of convergence of the two sets of beach ridges coincides with the areas of economically viable heavy mineral placers that resulted from the size and density based sorting under the repeated transgressive events to which the coast had subjected to. The chain of wetlands in the eastern side of the study area has been evolved from a mega lagoon existed during Late Pleistocene. The Pallikkal River that links discrete eastern wetland bodies has been evolved into its present form during Early Holocene.

Synthesis, spectral characterization, crystal structure, cytotoxicity and apoptosis-Inducing activity of two derivatives of 2-hydroxy-1,4-naphthaquinone.

A phenaxazone compound [5H-Benzo[a]phenoxazin-5-one (BP)] along with an aminoquinone[2-[(o-hydroxyphenyl)amino]-1,4-naphthaquinone (HAN)] derivatives were synthesized from lawsone using ultrasound irradiation technique. The structure of the compounds were characterized by elemental analysis and various spectral studies. Optoelectronic properties were studied using Schrodinger material science suit (2015). The compounds exhibit fluorescence emission in longer wave length it may find applications in photodynamic therapy. Single crystal X-ray diffraction studies reveals that the compound BP crystallizes in monoclinic space group. The antioxidant activity of HAN and BP were determined using DPPH radical scavenging assay and the results indicate that both the compounds have good antioxidant capacity, HAN having more scavenging activity than BP. Lead molecules were identified using in silico molecular docking studies as a green chemistry approach. iGEMDOCK, GOLD and Schrödinger softwares were used for these studies. The docking studies reveal that the structural modification of the parent compound gave more active compounds making them promising lead molecules. The lead molecules were subjected to in vitro studies. The cytotoxicity of BP and HAN was studied using human breast cancer (SKBR3) cell lines. The IC50 value of HAN was found to be 19.8µM while BP was found to have cell viability, less than 10% even at 25µM concentration. The chemotherapeutic agents kill the cancer cells mainly through apoptosis. HAN and BP were subjected to apoptosis studies. BP was found to more active than HAN. Thus it can be suggested that the mechanism of cell death may be through apoptosis.

Design and synthesis of cis-restricted benzimidazole and benzothiazole mimics of combretastatin A-4 as antimitotic agents with apoptosis inducing ability.

A series of colchicine site binding tubulin inhibitors were designed and synthesized by the modification of the combretastatin A-4 (CA4) pharmacophore. The ring B was replaced by the pharmacologically relevant benzimidazole or benzothiazole scaffolds, and the cis-configuration of the olefinic bond was restricted by the incorporation of a pyridine ring which is envisaged by the structural resemblance to a tubulin inhibitor like E7010. These compounds were evaluated for their antiproliferative activity on selected cancer cell lines and an insight in the structure activity relationship was developed. The most potent compounds (6c and 6l) demonstrated an antiproliferative effect comparable and superior to that of CA4 (GI50 up to 40nM). Mitotic cell cycle arrest in G2/M phase revealed the disruption of microtubule dynamics that was confirmed by tubulin polymerization assays and immunocytochemistry studies at the cellular level. The molecular docking studies suggested that the binding of these mimics at the colchicine site of the tubulin is similar to that of combretastatin A-4.

Tropical Peat and Peatland Development in the Floodplains of the Greater Pamba Basin, South-Western India during the Holocene.

Holocene sequences in the humid tropical region of Kerala, South-western (SW) India have preserved abundance of organic-rich sediments in the form of peat and its rapid development in a narrow time frame towards Middle Holocene has been found to be significant. The sub-coastal areas and flood plains of the Greater Pamba Basin have provided palaeorecords of peat indicating that the deposits are essentially formed within freshwater. The combination of factors like stabilized sea level and its subsequent fall since the Middle Holocene, topographic relief and climatic conditions led to rapid peat accumulation across the coastal lowlands. The high rainfall and massive floods coupled with a rising sea level must have inundated > 75% of the coastal plain land converting it into a veritable lagoon-lake system that eventually led to abrupt termination of the forest ecosystem and also converted the floodplains into peatland where accumulation of peat almost to 2.0-3.0 m thickness in coastal lowlands and river basins during the shorter interval in the Middle Holocene. Vast areas of the coastal plains of Kerala have been converted into carbon rich peatland during the Middle Holocene and transforming the entire coastal stretch and associated landforms as one of the relatively youngest peatlands in the extreme southern tip of India. Unlike the uninterrupted formation of peatlands of considerable extent during the Holocene in Southeast Asia, the south Peninsular Indian region has restricted and short intervals of peatlands in the floodplains and coastal lowlands. Such a scenario is attributed to the topographic relief of the terrain and the prevailing hydrological regimes and environmental conditions as a consequence of monsoon variability since Middle Holocene in SW India. Considering the tropical coastal lowlands and associated peatlands are excellent repositories of carbon, they are very important for regional carbon cycling and habitat diversity. The alarming rate of land modification and development is destabilizing these carbon pools resulting in large scale carbon emissions to the atmosphere and loss of low-latitude peat palaeorecords. Therefore, these palaeorecords are to be conserved and addressed for better understanding and utilizing the carbon pool for effective climate change adaptation. This communication is the first attempt of addressing the peat formation and peatland development during the Holocene from the tropical region of Peninsular India.

Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing.

INTRODUCTION: Brucellosis is one of the most important zoonotic diseases that affects multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country. METHODOLOGY: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST). RESULTS: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14. CONCLUSION: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.