RESUMEN
Streptococcus suis, a zoonotic bacterial pathogen circulated through swine, can cause severe infections in humans. Because human S. suis infections are not notifiable in most countries, incidence is underestimated. We aimed to increase insight into the molecular epidemiology of human S. suis infections in Europe. To procure data, we surveyed 7 reference laboratories and performed a systematic review of the scientific literature. We identified 236 cases of human S. suis infection from those sources and an additional 87 by scanning gray literature. We performed whole-genome sequencing to type 46 zoonotic S. suis isolates and combined them with 28 publicly available genomes in a core-genome phylogeny. Clonal complex (CC) 1 isolates accounted for 87% of typed human infections; CC20, CC25, CC87, and CC94 also caused infections. Emergence of diverse zoonotic clades and notable severity of illness in humans support classifying S. suis infection as a notifiable condition.
Asunto(s)
Epidemiología Molecular , Filogenia , Infecciones Estreptocócicas , Streptococcus suis , Zoonosis , Streptococcus suis/genética , Streptococcus suis/clasificación , Infecciones Estreptocócicas/epidemiología , Infecciones Estreptocócicas/microbiología , Infecciones Estreptocócicas/veterinaria , Europa (Continente)/epidemiología , Humanos , Animales , Zoonosis/epidemiología , Porcinos , Secuenciación Completa del Genoma , Enfermedades Transmisibles Emergentes/epidemiología , Enfermedades Transmisibles Emergentes/microbiología , Zoonosis Bacterianas/epidemiología , Zoonosis Bacterianas/microbiología , Enfermedades de los Porcinos/epidemiología , Enfermedades de los Porcinos/microbiologíaRESUMEN
Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20-36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya. The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, l-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis, strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75âmol%. Major fatty acids were C18:1 ω7c, cyclo C19â:â0 and C16â:â0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8â%) and Q10 (98.2â%). Digital DNA-DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis, respectively, while average nucleotide identity values were around 84â%. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T).
Asunto(s)
Ácidos Grasos , Noma , Humanos , Ácidos Grasos/química , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , ADN Bacteriano/genética , Técnicas de Tipificación Bacteriana , Composición de Base , BacteriasRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Adulto , Bacteriemia/diagnóstico , Bacteriemia/etiología , Cupriavidus/aislamiento & purificación , Colitis Ulcerosa/complicaciones , Bacteriemia/microbiología , Inmunosupresores/uso terapéutico , Bacilos y Cocos Aerobios Gramnegativos/aislamiento & purificación , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción , Imipenem/uso terapéuticoAsunto(s)
Antibacterianos/farmacología , Bacteriemia/diagnóstico , Chryseobacterium/aislamiento & purificación , Colistina/farmacología , Farmacorresistencia Bacteriana , Infecciones por Bacterias Gramnegativas/diagnóstico , Antibacterianos/administración & dosificación , Trastorno Autístico/complicaciones , Bacteriemia/microbiología , Bacteriemia/patología , Niño , Chryseobacterium/efectos de los fármacos , Ciprofloxacina/administración & dosificación , Femenino , Infecciones por Bacterias Gramnegativas/microbiología , Infecciones por Bacterias Gramnegativas/patología , Humanos , Resultado del Tratamiento , Combinación Trimetoprim y Sulfametoxazol/administración & dosificaciónRESUMEN
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals.
Asunto(s)
Farmacorresistencia Bacteriana/genética , Genotipo , Enfermedades de las Ovejas/epidemiología , Infecciones Estreptocócicas/epidemiología , Infecciones Estreptocócicas/veterinaria , Streptococcus pyogenes/genética , Animales , Antibacterianos/farmacología , Antígenos Bacterianos/genética , Antígenos Bacterianos/metabolismo , Proteínas de la Membrana Bacteriana Externa/genética , Proteínas de la Membrana Bacteriana Externa/metabolismo , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Células Clonales , Electroforesis en Gel de Campo Pulsado , Granjas , Expresión Génica , Macrólidos/farmacología , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismo , Pruebas de Sensibilidad Microbiana , Tipificación de Secuencias Multilocus , Fenotipo , Conejos , Ovinos/microbiología , Enfermedades de las Ovejas/tratamiento farmacológico , Enfermedades de las Ovejas/microbiología , Enfermedades de las Ovejas/transmisión , España , Infecciones Estreptocócicas/tratamiento farmacológico , Infecciones Estreptocócicas/transmisión , Streptococcus pyogenes/clasificación , Streptococcus pyogenes/efectos de los fármacos , Streptococcus pyogenes/aislamiento & purificaciónRESUMEN
BACKGROUND: Members of the genus Pseudonocardia have been widely reported and recovered from several ecosystems, such as soil samples and plant samples. Pseudonocardia bacteria colonize the microbial communities on the integument of fungus gardening ant species. We present the first documented case of Pseudonocardia carboxydivorans isolated in human cerebrospinal fluid (CSF). To the best of our knowledge, this is the first report of an human infection by P. carboxydivorans. CASE PRESENTATION: A patient, who suffered a traumatic brain injury a month before, was admitted to this hospital due to gait alteration and cognitive disturbances. Culture of cerebrospinal fluid showed ramified, not acid-fast, Gram positive bacilli. The bacterium was identified by molecular methods as P. carboxydivorans. CONCLUSION: This is the first documented case of isolating P. carboxydivorans in human CSF in a case of probable meningitis. Further research is needed in order to determine its pathogenic role in human infections.
Asunto(s)
Actinobacteria/aislamiento & purificación , Lesiones Traumáticas del Encéfalo/complicaciones , Infecciones por Bacterias Grampositivas/líquido cefalorraquídeo , Actinobacteria/genética , Actinobacteria/patogenicidad , Anciano , Lesiones Traumáticas del Encéfalo/microbiología , Líquido Cefalorraquídeo/microbiología , Infecciones por Bacterias Grampositivas/tratamiento farmacológico , Infecciones por Bacterias Grampositivas/etiología , Infecciones por Bacterias Grampositivas/microbiología , Humanos , MasculinoRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Persona de Mediana Edad , Peritonitis/complicaciones , Peritonitis/diagnóstico , Peritonitis/tratamiento farmacológico , Streptococcus equi , Streptococcus equi/aislamiento & purificación , Paracentesis/métodos , Aztreonam/uso terapéutico , Metronidazol/uso terapéutico , Pruebas de Sensibilidad Microbiana/métodos , Levofloxacino/uso terapéutico , Colangiografía , Constricción Patológica/complicaciones , Constricción PatológicaRESUMEN
Christensenella minuta is a Gram-negative strictly anaerobic short rod that inhabits the human gut. This bacterium was isolated in a mixed infection with Desulfovibrio desulfuricansfrom the blood of a patient with a diagnosis of acute appendicitis. The strain was identified by 16S rRNA sequence analysis. As far as we know, this is the first time C.minuta has been isolated from a human clinical specimen.
Asunto(s)
Apendicitis/diagnóstico , Bacteriemia/diagnóstico , Clostridiales/aislamiento & purificación , Coinfección/diagnóstico , Infecciones por Bacterias Grampositivas/diagnóstico , Adolescente , Apendicitis/complicaciones , Apendicitis/microbiología , Bacteriemia/microbiología , Bacteriemia/patología , Clostridiales/clasificación , Clostridiales/genética , Coinfección/microbiología , Coinfección/patología , ADN Bacteriano/química , ADN Bacteriano/genética , ADN Ribosómico/química , ADN Ribosómico/genética , Desulfovibrio desulfuricans/clasificación , Desulfovibrio desulfuricans/genética , Desulfovibrio desulfuricans/aislamiento & purificación , Infecciones por Desulfovibrionaceae/diagnóstico , Infecciones por Desulfovibrionaceae/microbiología , Infecciones por Desulfovibrionaceae/patología , Infecciones por Bacterias Grampositivas/microbiología , Infecciones por Bacterias Grampositivas/patología , Humanos , Masculino , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADNRESUMEN
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin --#91;1 mef(A), 3 erm(TR), 1 mef(A) + erm(TR) and 1 erm(TR) + erm(B)--#93; and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline --#91;2 tet(M), 5 tet(M) + tet(O)--#93;. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLS B phenotype.
Streptococcus dysgalactiae subsp. equisimilis (SDSE) posee factores de virulencia similares a Streptococcus pyogenes y, en consecuencia, produce faringitis e infecciones graves indistinguibles de las generadas por este patógeno clásico. Los objetivos del estudio fueron conocer la prevalencia de SDSE en infecciones invasivas en Argentina, estudiar su diversidad genética, determinar la presencia de genes de virulencia, ensayar su sensibilidad a los antibióticos y conocer los genes de resistencia. Se emplearon métodos convencionales de identificación. La sensibilidad se determinó por difusión, Etest y dilución en agar. Participaron 28 centros de 16 ciudades argentinas. Se obtuvieron 23 aislamientos (16 del grupo G y 7 del grupo C) desde el 1-7-2011 hasta el 30-6-2012. Se registraron 2 muertes en adultos (8,7%). La mayoría de los aislamientos fueron obtenidos de sangre (60,9%). Todos eran portadores de los genes speJ y ssa. Los genotipos más frecuentes fueron stG62647, stG653 y stG840. Se detectaron 19 pulsotipos distintos. Todos los aislamientos fueron sensibles a penicilina y levofloxacina, 6 (26,1%) presentaron resistencia o sensibilidad disminuida a eritromicina (1 mef--#91;A--#93;, 3 erm--#91;TR--#93;, 1 mef--#91;A--#93; + erm--#91;TR--#93; y 1 erm--#91;TR--#93; + erm--#91;B--#93;) y 7 (30,4%) fueron resistentes o tuvieron sensibilidad disminuida a tetraciclina (2 tet--#91;M--#93;, 5 tet--#91;M--#93; + tet--#91;O--#93;). La prevalencia anual en la Argentina fue de al menos 23 infecciones invasivas por SDSE y se observó una amplia diversidad genética. Todos los aislamientos presentaron los genes ssa y speJ. Como en otros estudios, la resistencia a macrólidos (26,1%) estuvo asociada, principalmente, al fenotipo MLS B.
Asunto(s)
Humanos , Masculino , Femenino , Infecciones Estreptocócicas/clasificación , Streptococcus/aislamiento & purificación , Streptococcus/patogenicidad , Argentina , Streptococcus/genética , Farmacorresistencia Microbiana , Estudios Transversales/métodosRESUMEN
No disponible
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Humanos , Masculino , Persona de Mediana Edad , Bordetella/aislamiento & purificación , Infecciones por Bordetella/complicaciones , Infecciones por Bordetella/tratamiento farmacológico , Infecciones por Bordetella/patología , Pruebas de Sensibilidad Microbiana/métodos , Pruebas de Sensibilidad Microbiana , Fibrosis Quística/complicaciones , 51426 , Combinación Trimetoprim y Sulfametoxazol/uso terapéutico , Penicilinas/uso terapéutico , Cefalosporinas/uso terapéutico , Minociclina/uso terapéuticoRESUMEN
No disponible
Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Bacteria Gordonia , Bacteria Gordonia/aislamiento & purificación , Absceso/complicaciones , Absceso/diagnóstico , Absceso/tratamiento farmacológico , Combinación Amoxicilina-Clavulanato de Potasio/uso terapéutico , Agar/análisis , Antiinfecciosos/uso terapéutico , Esputo/citología , Esputo/microbiologíaRESUMEN
Human infections caused by Streptococcus lactarius have not been previously reported. In the present report, we describe a lactational mastitis caused by this organism. The infection occurred in a 28-year-old breast-feeding female, with a 10-days history of moderate pain on the right breast. The patient was cured after antibiotic treatment with levofloxacin for 21 days. Our case shows that S. lactarius should be considered as a cause of lactational mastitis. The introduction of molecular microbiology techniques can be extremely useful for knowing the implication of streptococci in lactational mastitis.
Asunto(s)
Mastitis/etiología , Mastitis/patología , Infecciones Estreptocócicas/diagnóstico , Infecciones Estreptocócicas/patología , Streptococcus/clasificación , Streptococcus/aislamiento & purificación , Adulto , Antibacterianos/uso terapéutico , Femenino , Humanos , Levofloxacino/uso terapéutico , Mastitis/tratamiento farmacológico , Mastitis/microbiología , Técnicas de Diagnóstico Molecular , Infecciones Estreptocócicas/tratamiento farmacológico , Infecciones Estreptocócicas/microbiología , Resultado del TratamientoRESUMEN
No disponible
Asunto(s)
Humanos , Femenino , Persona de Mediana Edad , Nocardia/aislamiento & purificación , Nocardiosis/microbiología , Inyecciones Intralesiones/efectos adversos , Osteoartritis/tratamiento farmacológicoRESUMEN
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin [1 mef(A), 3 erm(TR), 1 mef(A)+erm(TR) and 1 erm(TR)+erm(B)] and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline [2 tet(M), 5 tet(M)+tet(O)]. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLSB phenotype.
Asunto(s)
Bacteriemia/microbiología , Infecciones Estreptocócicas/microbiología , Streptococcus/genética , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Argentina/epidemiología , Artritis Infecciosa/epidemiología , Artritis Infecciosa/microbiología , Bacteriemia/epidemiología , Líquidos Corporales/microbiología , Niño , Preescolar , ADN Bacteriano/genética , Farmacorresistencia Bacteriana Múltiple/genética , Electroforesis en Gel de Campo Pulsado , Femenino , Genes Bacterianos , Variación Genética , Humanos , Lactante , Masculino , Persona de Mediana Edad , Prevalencia , Infecciones de los Tejidos Blandos/epidemiología , Infecciones de los Tejidos Blandos/microbiología , Infecciones Estreptocócicas/epidemiología , Streptococcus/efectos de los fármacos , Streptococcus/aislamiento & purificación , Streptococcus/patogenicidad , Streptococcus pyogenes/genética , Streptococcus pyogenes/aislamiento & purificación , Virulencia , Adulto JovenRESUMEN
BACKGROUND: We describe a case of traumatic ocular endophthalmitis caused by Nocardia kruczakiae after vegetable trauma in an immunocompetent child. FINDINGS: A 5-year-old boy suffered from a trauma with a palm tree leaflet. Two months later, he was diagnosed with traumatic infectious uveitis and intumescent cataract with anterior capsule rupture. Intensive treatment with systemic and topical vancomycin, ceftazidime and methylprednisolone began. After 1 month, he underwent phacoemulsification with intraocular lens implantation (IOL). After some episodes of reactivation, he was diagnosed with traumatic nocardial endophthalmitis from aqueous humour samples. Several operations and specific antibiotic therapy resolved the infection. CONCLUSIONS: In cases of traumatic endophthalmitis and several recurrences, it is extremely useful to make an etiologic diagnosis in order to treat the patient with specific antibiotics.