Relationship between lifespan and somatic mutation in D. melanogaster after treatment with chlorophyllin.

Sodium copper chlorophyllin (SCC) has a genetic damage inhibitory capacity due to its antioxidant action. For this reason, it was considered to investigate its role in the life span of Drosophila melanogaster and its relationship with the frequency of somatic mutation induced by gamma rays. Results indicated that SCC alone prolonged the lifespan only in females, but in combination with 20 Gy of gamma rays, the aging delay in both sexes was significant. In addition to confirming that the porphyrin reduces the frequency of mutation, the individuals with the highest mutation load are the individuals who die more quickly, and once they are eliminated, the survivor individuals treated with 20 Gy or with SCC + 20 Gy, died at the same rate. The results together indicate that SCC not only inhibits induced genetic damage, but it also has beneficial effects that probably cause an aging delay of the treated population that need to be investigated.

Evidence that the radioprotector effect of ascorbic acid depends on the radiation dose rate.

Many studies have revealed that ascorbic acid (Aa) acts as a powerful inhibitor of genetic damage. The objetive of the present study was to evaluate the radioprotector effect of Aa at two diferent radiation dose rates. The somatic mutation and recombination test in Drosophila melanogaster was used. 48 h larvae were treated for 24 h with 25, 50 and 100 mM of Aa. After pretreatment, larvae were irradiated with 20 Gy of gamma rays administered at 36 or 960 Gy/h. Toxicity, development rate and frequency of mutant spots were recorded. Results provide evidence of a radioprotective effect for all tested concentrations of Aa only when 20 Gy were delivered at 36 Gy/h and only with 25 mM using the 960 Gy/h. To consider the use of Aa as radioprotector or therapeutic agent, it is necessary to know its potential under different situations to avoid unwanted injuries.

Síntomas prodrómicos del espectro obsesivo-compulsivo en un caso en esquizofrenia / No disponible

No disponible

Metástasis cerebral de un cáncer mamario en paciente joven / Cerebral metastasis of a breast cancer in a young patient

Se presentó una paciente de 30 años de edad con cáncer de mama derecha combinado, que evoluciona tórpidamente con recidiva tumoral y metástasis cerebral en corto tiempo. Se presentan ilustraciones radiológicas y se revisa el tema(AU)

Authors present the case of a female patient aged 30 with combined right breast cancer which evolves torpidly with tumor relapse and short-term brain metastasis. Radiologic illustrations are showed and a review on this subject(AU)

Metástasis cerebral de un cáncer mamario en paciente joven / Cerebral metastasis of a breast cancer in a young patient

Se presentó una paciente de 30 años de edad con cáncer de mama derecha combinado, que evoluciona tórpidamente con recidiva tumoral y metástasis cerebral en corto tiempo. Se presentan ilustraciones radiológicas y se revisa el tema

Authors present the case of a female patient aged 30 with combined right breast cancer which evolves torpidly with tumor relapse and short-term brain metastasis. Radiologic illustrations are showed and a review on this subject

Empty sella and primary autoimmune hypothyroidism.

In order to assess the association between empty sella (ES) and primary autoimmune hypothyroidism, and the possibility of a common pathogenesis. We retrospectively studied all patients with presumed ES diagnosed in the last 20 years, most of whom were treated by our Endocrinology Department. Subjects with a known etiology were excluded. Incomplete records or those with a doubtful diagnosis were also excluded. A total of 56 subjects were included in the study. ES was diagnosed by pituitary MRI. The measurement of free T4, TSH, and antithyroid antibodies (TPOAb and TgAb) was assayed using commercial kits. The cases of hypothyroidism obtained were compared with those in another group of similar patients, diagnosed with diabetes mellitus type 2, through chi2 test. A total of 15 (26.78%) patients of 56 with ES had autoimmune thyroid disease (subclinical or clinical hypothyroidism). Primary hypothyroidism with negative antithyroid autoantibodies was found in a further 13 patients (23.21%). The 46.42% of ES had primary hypothyroidism; this result had obtained a statistically significant difference when compared to the ratio obtained in the group of diabetes mellitus type 2 (P < 0.0029). There is an important association between ES and autoimmune thyroid disease, which reached 26.78% in our series. We suggest the possibility of a common pathogenesis for certain cases of ES and autoimmune thyroid disease, with the end point of ES in the pituitary, and atrophy in the thyroid gland.

Aeromonas detection and their toxins from drinking water from reservoirs and drinking fountains.

Aeromonads are inhabitants of aquatic ecosystems and are described as being involved in intestinal disturbances and other infections. A total of 200 drinking water samples from domestic and public reservoirs and drinking fountains located in São Paulo (Brazil), were analyzed for the presence of Aeromonas. Samples were concentrated by membrane filtration and enriched in APW. ADA medium was used for Aeromonas isolation and colonies were confirmed by biochemical characterization. Strains isolated were tested for hemolysin and toxin production. Aeromonas was detected in 12 samples (6.0%). Aeromonas strains (96) were isolated and identified as: A. caviae (41.7%), A. hydrophila (15.7%), A.allosacharophila (10.4%), A. schubertii (1.0%) and Aeromonas spp. (31.2%). The results revealed that 70% of A. caviae, 66.7% of A. hydrophila, 80% of A. allosacharophila and 46.6% of Aeromonas spp. were hemolytic. The assay for checking production of toxins showed that 17.5% of A. caviae, 73.3% of A. hydrophila, 60% of A. allosacharophila, 100% of A. schubertii, and 33.3% of Aeromonas spp. were able to produce toxins. The results demonstrated the pathogenic potential of Aeromonas, indicating that the presence of this emerging pathogen in water systems is a public health concern.

Hamartoma de la mama / Breast hamartoma

El hamartoma de la mama o fibroadenolipoma de la mama es una lesión nodular que puede simular un fibroadenoma o un tumor phyllodes. Aparece en las mujeres en la edad media de la vida y se presenta clínicamente como un nódulo bien definido e indoloro. El diagnóstico imagenológico es el que hace pensar en éste, ya que es un tumor poco frecuente. Histológicamente cumple los requisitos generales de un hamartoma: mezcla desordenada de tejidos maduros y especializados. En la mama se puede apreciar estructura ductal y lobulillar con estroma fibroso, tejido adiposo y en ocasiones, tejido muscular liso. En este trabajo se presenta un caso de una paciente de 40 años con un hamartoma de la mama derecha que histológicamente mostraba además, tejido óseo y cartilaginoso(AU)

Hamartoma de la mama / Breast hamartoma

El hamartoma de la mama o fibroadenolipoma de la mama es una lesión nodular que puede simular un fibroadenoma o un tumor phyllodes. Aparece en las mujeres en la edad media de la vida y se presenta clínicamente como un nódulo bien definido e indoloro. El diagnóstico imagenológico es el que hace pensar en éste, ya que es un tumor poco frecuente. Histológicamente cumple los requisitos generales de un hamartoma: mezcla desordenada de tejidos maduros y especializados. En la mama se puede apreciar estructura ductal y lobulillar con estroma fibroso, tejido adiposo y en ocasiones, tejido muscular liso. En este trabajo se presenta un caso de una paciente de 40 años con un hamartoma de la mama derecha que histológicamente mostraba además, tejido óseo y cartilaginoso(AU)

Breast hamartoma or breast fibroadenolipoma is a nodular lesion that may resemble a fibroadenoma or phyllodes tumor. It appears in middle-aged women and it clinically presents as a well defined and painless nodule. The imaging diagnosis makes to think about it, since it is an uncommon tumor. From the histological point of view, it fulfills the general requirements of a hamartoma: disarranged combination of mature and specialized tissues. Ductal and lobular structure with fibrous stroma, fat tissue and, on occasions, smooth muscular tissue, may be observed in the breast. A case of a 40-year-old female patient with a hamartoma of the right mamma that histologically showed osteal and cartilaginous tissue is reported(AU)

Los criterios de calidad del agua potable en diferentes países: Estados Unidos, Unión Europea, Brasil, Chile

En este trabajo se presenta la reglamentación a seguir en Brasil, para determinar la potabilidad del agua para consumo humano, cotejando con la de distintos países

Los nuevos problemas microbiológicos que afectan la calidad del agua potable

Aquí la autora explica el papel del agua en la transmisión de enfermedades en especial cuando existe contaminación por materias fecales

Nuevos problemas microbiológicos que afectan la calidad del agua potable

Aquí la autora explica el papel del agua en la transmición de enfermedades en especial cuando existe contaminación por materias fecales

Criterios de calidad del agua potable en diferentes países: Estados Unidos, Unión Europea, Brasil, Chile

En este trabajo se presenta la reglamentación a seguir en Brasil, para determinar la potabilidad del agua para consumo humano, cotejando con la de distintos países

Survival of culturable Vibrio cholerae 01 and non 01 in seawater, freshwater and wastewater and effect of the water environment on enterotoxin production

Um estudo sobre a sobrevivência de V. chlolerae O1 biotipo clássico. V. cholerae O1 El Tor e V. cholerae näo-O1 em microcosmos contendo água doce, água do mar e águas de esgoto do Estado de Säo Paulo, Brasil, foi efetuado. O efeito dos fatores físicos-químicos do meio sobre a expressäo da enterotoxina dos vibrios foi analisado. As três cepas de V. cholerae testadas apresentaram melhor sobrevivência em água previamente esterilizada por filtraçäo confirmando que a competiçäo é um fator interferente na sobrevivência dos vibrios. A sobrevivência foi superior quando o estudo foi efetuado na temperatura de 10ºC ou 35ºC, e foi tambem influenciada pela alcalinidade, pH e concentraçöes de cloreto, sódio e potássio. O maior tempo de sobrevivência foi observado em água do mar näo poluída confirmando que estes vibrios säo autóctones no ambiente marinho. A toxigenicidade de V. cholorea O1 clássico e E1 Tor näo foi afetada durante o período de experimento

Tratamiento del asma bronquial en crisis, con fenoterol y furosemida nebulizados: estudio prospectivo a doble ciego / Treatment of bronchial asthma on crisis with nebulized fenoterol and furosemida: prospective double blind study

El propósito de este estudio fué evaluar el efecto del fenoterol y la furosemida nebulizados sobre las crisis asmáticas. Treinta pacientes con asma leve o moderada fueron admitidos en un estudio doble ciego, que la furosemida nebulizada podría jugar un papel importante en el tratamiento de las crisis asmáticas leves y moderadas

Isolamento de Salmonella no ambiente aquático: significado sanitário / Isolation of Salmonella from aquatic environment: sanitary significance

Para avaliar o significado sanitário de Salmonella em águas foram examinadas 894 amostras de águas de esgotos, salgadas e doces, sendo obtidos 5.430 isolamentos de Salmonella. Constatou-se que, nos esgotos os sorogrupos e sorotipos de Salmonella, com exceçäo de S. agona, apresentaram padräo de distribuiçäo similar ao obtido em coprocultura. Nos esgotos provenientes de hospitais predominou S. typhimurium var. 0:5- que também foi o mais frequentemente isolado de coproculturas. Foi observado que nos mananciais e praias mais poluidos, prevaleciam os sorogrupos e sorotipos mais comuns de esgotos, e o contrário ocorreu em amostras de locais menos poluídos. Näo foi obtida correlaçäo estatística entre NMP/100 mL salmonelas e NMP/100 mL de coliformes em esgoto, no entanto, quando se relacionou graficamente, níveis de coliformes e porcentagem de isolamento de salmonelas em águas doces e salgadas, obteve-se uma relaçäo praticamente linear

Eficácia do tratamento químico e término na desnutriçäo de patógenos em lodo digerido / Efficacy of chemical and thermal treatment in the destrution of pathogens in digested sludge

Amostras de lodos de esgoto digeridos, submetidas a tratamento químico (cloreto férrico e cal) e a tratamento térmico (50-C a 115-C durante 5 a 15 minutos), foram analisadas quanto à presença de indicadores convenvionais de poluiçäo fecal e patógenos. Amostras de lodos digeridos brutos apresentaram alta incidência de indicadores e patógenos. Após tratamento químico obteve-se reduçäo de até 99,9% de coliformes fecais, 99,6% de estreptococos fecais, 98,7% de salmonelas, e 99,6% de ovos de helmintos. O tratameno térmico foi menos eficiente para eliminaçäo de bactérias e de ovos de helmintos. Enterovírus foram totalmente inativados no tratamento térmico, porém no tratamento químico, somente quando quando o pH atingiu valor 12,0 ou superior

Isolamento de três novos sorotipos de Salmonella: S. Cotia, S. Guarapiranga e S. arizonae de águas de superfície, em São Paulo, Brasil / Isolation of three new Salmonella serotypes: S. Cotia, S. Guarapiranga and S. arizonae from water reservoirs in Sao Paulo city, Brasil

É relatado o isolamento de três novos sorotipos de Salmonella: S. cotia e S. guarapiranga, pertencentes ao subgênero I, e S. arizonae 65:I,v:z 35, pertencente ao subgênero Ill (AU).