Evolution of coherence singularities in polarization singular beams.

The evolution of correlation singularities in partially coherent polarization singular beams (PC-PSBs) is investigated. Since PSBs are the superposition of two orthogonally polarized vortex beams, the occurrence of coherence singularities in PC-PSBs is strongly governed by the topological charge of the component vortex beams and the spatial coherence length. Coherence singularities appear in the form of ring dislocations in the modulus of the spectral degree of coherence (SDoC) profile, and the number of ring dislocations is equal to the higher value of the topological charge of the superposing vortex beam. Furthermore, the SDoC phase profile can be used to determine the polarity of a PC-PSB. The findings of the study could be valuable in various applications that rely on the spatial coherence of beams, such as free-space communication and imaging.

Fano Resonance-Assisted All-Dielectric Array for Enhanced Near-Field Optical Trapping of Nanoparticles.

Near-field optics can overcome the diffraction limit by creating strong optical gradients to enable the trapping of nanoparticles. However, it remains challenging to achieve efficient, stable trapping without heating and thermal effects. Dielectric structures have been used to address this issue but usually offer weak trap stiffness. In this work, we exploit the Fano resonance effect in an all-dielectric quadrupole nanostructure to realize a 20-fold enhancement of trap stiffness, compared to the off-resonance case. This enables a high effective trap stiffness of 1.19 fN/nm for 100 nm diameter polystyrene nanoparticles with 4.2 mW/µm2 illumination. Furthermore, we demonstrate the capability of the structure to simultaneously trap two particles at distinct locations within the nanostructure array.

Coherence-induced depolarization effects in polarization singular beams.

We demonstrate theoretically and experimentally coherence-induced depolarization effects in generic and higher index polarization singular beams endowed with C-point (or V-point) polarization singularity. The irradiance profiles and degree of polarization (DoP) distributions are found to be governed by spatial coherence length, polarization singularity index, and orbital angular momentum (OAM) of the superposition states of the beams. On reducing the coherence length, the DoP distribution in the V-point deteriorates uniformly. In contrast, C-point beams resist depolarization exhibiting anti-depolarization around the central core of the beam due to the nonzero net OAM of the beam. Interestingly, the polarization vortex structure remains preserved on reducing the spatial coherence length.

Investigation of partially coherent vector vortex beams with non-isotropic states of spatial correlation.

In this work, the far-field properties of non-isotropic partially coherent vector vortex beams (PCVVBs) are investigated both theoretically and experimentally. The term non-isotropic signifies that the spatial correlations between the parallel and orthogonal electric field components are distinguishable. It is found that self-orientation and shaping of intensity profile, correlation-induced polarization and depolarization are highly dependent on both the non-isotropic correlation parameters and Poincaré-Hopf index (PHI) of the beam. The simultaneous depolarization and polarization effects are due to the difference in the input correlation parameters that alter the state of polarization (SOP) and degree of polarization (DOP) distributions. The experimental results are in good agreement with the theoretical predictions. The distinguishability of correlation parameters at the source plane leads to significant changes on its intensity profile, DOP, and SOP distributions on far-field propagation, which may found potential applications in beam shaping, detecting and imaging atmospheric lidar, optical imaging and directional transportation where the self-rotation characteristic of beam plays an important role.

Disrupted autophagy and neuronal dysfunction in C. elegans knockin models of FUS amyotrophic lateral sclerosis.

How mutations in FUS lead to neuronal dysfunction in amyotrophic lateral sclerosis (ALS) patients remains unclear. To examine mechanisms underlying ALS FUS dysfunction, we generate C. elegans knockin models using CRISPR-Cas9-mediated genome editing, creating R524S and P525L ALS FUS models. Although FUS inclusions are not detected, ALS FUS animals show defective neuromuscular function and locomotion under stress. Unlike animals lacking the endogenous FUS ortholog, ALS FUS animals have impaired neuronal autophagy and increased SQST-1 accumulation in motor neurons. Loss of sqst-1, the C. elegans ortholog for ALS-linked, autophagy adaptor protein SQSTM1/p62, suppresses both neuromuscular and stress-induced locomotion defects in ALS FUS animals, but does not suppress neuronal autophagy defects. Therefore, autophagy dysfunction is upstream of, and not dependent on, SQSTM1 function in ALS FUS pathogenesis. Combined, our findings demonstrate that autophagy dysfunction likely contributes to protein homeostasis and neuromuscular defects in ALS FUS knockin animals.

Young's double-slit experiment with vector vortex beams.

In this Letter, Young's double-slit experiment with vector vortex beams is investigated. We present the results for various Poincaré-Hopf index beams of this class considering all four major types. Polarization associated morphological changes in the far-field interference pattern are studied both theoretically and experimentally. The Fraunhofer pattern consists of lattices of polarization singularities of the generic type, located on a line, in a direction perpendicular to the slit. The number of linear lattices varies as a function of Poincaré-Hopf index η of the beam that is diffracted, and the number of intensity nulls occurring along the vertical line is equal to |η|.

Detection of degenerate Stokes index states.

Stokes phase is the phase difference between orthogonal component states in the decomposition of any polarization state. Phase singularities in the Stokes phase distribution are Stokes singularities of an inhomogeneous polarization distribution. Under circular decomposition, Stokes phase distribution [Formula: see text] represents polarization azimuth [Formula: see text] distribution and the singularities present in it are polarization singularities. Therefore, the charge of the Stokes vortices depicted as Stokes index [Formula: see text] is an important parameter associated with the polarization singularity. The Hybrid order Poincaré sphere (HyOPS)/Higher order Poincaré sphere (HOPS) beams, all having same Stokes index, contain a Stokes singularity at the center of the beam as these beams are constructed by vortex superposition. These beams, being superposition of orthogonal orbital angular momentum (OAM) states in orthogonal spin angular momentum (SAM) states can offer great multiplexing capabilities in communication. In this article, we identify these degenerate Stokes index states and discuss the ways and means of lifting this degeneracy. Otherwise, there are limitations on intensity based detection techniques, where demultiplexing or segregation of different HOPS/HyOPS beams is warranted. The method adduced here uses the diffraction of these beams through an equilateral triangular aperture in combination with polarization transformation as a probe to lift the Stokes index/Stokes phase degeneracy. Successively, the novelty of the detection scheme is discussed in the context of beams with alike polarization distributions where even the technique of Stokes polarimetry fails to predict the OAM and SAM content of the beam.

Coherence-induced polarization effects in vector vortex beams.

We demonstrate theoretically and experimentally coherence-induced polarization changes in higher-order vector vortex beams (VVBs) with polarization singularity. The prominent depolarization on decreasing the transverse correlation width in a focused partially coherent VVB provides a means to shape the intensity profile and degree of polarization (DOP) while preserving the polarization distribution. The intensity variation and DOP dip are found to be dependent on the polarization singularity index of the beam. Our results may provide an additional degree of freedom in myriad applications presently projected with VVBs.

Discriminating between sleep and exercise-induced fatigue using computer vision and behavioral genetics.

Following prolonged swimming, Caenorhabditis elegans cycle between active swimming bouts and inactive quiescent bouts. Swimming is exercise for C. elegans and here we suggest that inactive bouts are a recovery state akin to fatigue. It is known that cGMP-dependent kinase (PKG) activity plays a conserved role in sleep, rest, and arousal. Using C. elegans EGL-4 PKG, we first validate a novel learning-based computer vision approach to automatically analyze C. elegans locomotory behavior and an edge detection program that is able to distinguish between activity and inactivity during swimming for long periods of time. We find that C. elegans EGL-4 PKG function impacts timing of exercise-induced quiescent (EIQ) bout onset, fractional quiescence, bout number, and bout duration, suggesting that previously described pathways are engaged during EIQ bouts. However, EIQ bouts are likely not sleep as animals are feeding during the majority of EIQ bouts. We find that genetic perturbation of neurons required for other C. elegans sleep states also does not alter EIQ dynamics. Additionally, we find that EIQ onset is sensitive to age and DAF-16 FOXO function. In summary, we have validated behavioral analysis software that enables a quantitative and detailed assessment of swimming behavior, including EIQ. We found novel EIQ defects in aged animals and animals with mutations in a gene involved in stress tolerance. We anticipate that further use of this software will facilitate the analysis of genes and pathways critical for fatigue and other C. elegans behaviors.

Properties and characteristics of nanocrystalline cellulose isolated from olive fiber.

Olive fiber is a sustainable material as well as alternative biomass for extraction of nanocrystalline cellulose (NCC), which has been widely applied in various industries. In the present study, ONC-I, ONC-II, and ONC-III were extracted from olive stem fiber at different hydrolysis reaction times of 30 min, 45 min, and 60 min, respectively. The nanoparticle size was found gradually reducing from ONC-I (11.35 nm width, 168.28 nm length) to ONC-III (6.92 nm width, 124.16 nm length) due to the disintegration of cellulose fibrils. ONC-II and ONC-III possessed highly pure cellulose compartments and enhanced crystals structure. This study also showed that rigidity increased from ONC-I to ONC-II. ONC-III showed the highest crystallinity of 83.1 %, endowing it as a potentially reliable load-bearing agent. Moreover, ONC-III exhibited highest stable heat resistance among the chemically-isolated nanocellulose. We concluded that olive NCC could be promising materials for a variety of industrial applications in various fields.

Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans.

Animal behaviors are commonly organized into long-lasting states that coordinately impact the generation of diverse motor outputs such as feeding, locomotion, and grooming. However, the neural mechanisms that coordinate these distinct motor programs remain poorly understood. Here, we examine how the distinct motor programs of the nematode C. elegans are coupled together across behavioral states. We describe a new imaging platform that permits automated, simultaneous quantification of each of the main C. elegans motor programs over hours or days. Analysis of these whole-organism behavioral profiles shows that the motor programs coordinately change as animals switch behavioral states. Utilizing genetics, optogenetics, and calcium imaging, we identify a new role for dopamine in coupling locomotion and egg-laying together across states. These results provide new insights into how the diverse motor programs throughout an organism are coordinated and suggest that neuromodulators like dopamine can couple motor circuits together in a state-dependent manner.

Animals generate many different motor programs (such as moving, feeding and grooming) that they can alter in response to internal needs and environmental cues. These motor programs are controlled by dedicated brain circuits that act on specific muscle groups. However, little is known about how organisms coordinate these different motor programs to ensure that their resulting behavior is coherent and appropriate to the situation. This is difficult to investigate in large organisms with complex nervous systems, but with 302 brain cells that control 143 muscle cells, the small worm Caenorhabditis elegans provides a good system to examine this question. Here, Cermak, Yu, Clark et al. devised imaging methods to record each type of motor program in C. elegans worms over long time periods, while also dissecting the underlying neural mechanisms that coordinate these motor programs. This constitutes one of the first efforts to capture and quantify all the behavioral outputs of an entire organism at once. The experiments also showed that dopamine ­ a messenger molecule in the brain ­ links the neural circuits that control two motor programs: movement and egg-laying. A specific type of high-speed movement activates brain cells that release dopamine, which then transmits this information to the egg-laying circuit. This means that worms lay most of their eggs whilst traveling at high speed through a food source, so that their progeny can be distributed across a nutritive environment. This work opens up the possibility to study how behaviors are coordinated at the level of the whole organism ­ a departure from the traditional way of focusing on how specific neural circuits generate specific behaviors. Ultimately, it will also be interesting to look at the role of dopamine in behavior coordination in a wide range of animals.

Posttreatment Imaging in Patients with Head and Neck Cancer without Clinical Evidence of Recurrence: Should Surveillance Imaging Extend Beyond 6 Months?

BACKGROUND AND PURPOSE: Early detection of residual or recurrent disease is important for effective salvage treatment in patients with head and neck cancer. Current National Comprehensive Cancer Network guidelines do not recommend standard surveillance imaging beyond 6 months unless there are worrisome signs or symptoms on clinical examination and offer vague guidelines for imaging of high-risk patients beyond that timeframe. Our goal was to evaluate the frequency of clinically occult recurrence in patients with head and neck squamous cell carcinoma with positive imaging findings (Neck Imaging Reporting and Data Systems scores of 2-4), especially after 6 months. MATERIALS AND METHODS: This institutional review board-approved, retrospective data base search queried neck CT reports with Neck Imaging Reporting and Data Systems scores of 2-4 from June 2014 to March 2018. The electronic medical records were reviewed to determine outcomes of clinical and radiologic follow-up, including symptoms, physical examination findings, pathologic correlation, and clinical notes within 3 months of imaging. RESULTS: A total of 255 cases, all with Neck Imaging Reporting and Data Systems scores of 2 or 3, met the inclusion criteria. Fifty-nine patients (23%) demonstrated recurrence (45 biopsy-proven, 14 based on clinical and imaging progression), and 21 patients (36%) had clinically occult recurrence (ie, no clinical evidence of disease at the time of the imaging examination). The median overall time to radiologically detected, clinically occult recurrence was 11.4 months from treatment completion. CONCLUSIONS: Imaging surveillance beyond the first posttreatment baseline study was critical for detecting clinically occult recurrent disease in patients with head and neck squamous cell carcinoma. More than one-third of all recurrences were seen in patients without clinical evidence of disease; and 81% of clinically occult recurrences occurred beyond 6 months.

Effect of cellulose nano fibers and nano clays on the mechanical, morphological, thermal and dynamic mechanical performance of kenaf/epoxy composites.

The aim of the present research work has focused on investigating the effect of cellulose nanofibers (CNFs) and nano clays (montmorillonite (MMT) & organoclay (OMMT)) at 0.75Wt % on the performance of kenaf/epoxy composites. Mechanical (tensile and flexural) and thermal properties of composites in terms of morphology, thermal stability, weight loss, and dynamic mechanical properties were analyzed. The obtained results revealed that the integration of stiff CNFs as filler enhanced the mechanical and thermal properties, storage and loss modulus while a considerable decrease in Tan δ was realized compared to kenaf/epoxy composites. Enhancement in the properties was observed for OMMT and CNFs composites compared to MMT/kenaf/epoxy composites, which is attributed to the uniform filler distribution and interfacial adhesion between CNFs, OMMT, kenaf and epoxy matrix. The obtained results revealed that OMMT and CNFs based kenaf/epoxy composites can be an efficient alternative for construction applications.

Characterization of microcrystalline cellulose extracted from olive fiber.

Olive fiber is a renewable natural fiber which has potential as an alternative biomass for extraction of microcrystalline cellulose (MCC). MCC has been widely applied in various industries owing to its small dimensional size for ease of reactive fabrication process. At present study, a serial treatments of bleaching, alkaline and acid hydrolysis was employed to extract OL-BLF, OL-PUF, and OL-MCC respectively from olive stem fiber. In morphology examination, a feature of short micro-crystallite particles was obtained for OL-MCC. The particle size was found gradually reducing from OL-PUF (305.31 µm) to OL-MCC (156.06 µm) due to the disintegration of cellulose fibrils. From physicochemical analysis, most lignin and hemicellulose components had been removed from OL-BLF to form OL-PUF with individually fibril structure. The elemental analysis revealed that highly pure cellulose component was obtained for OL-MCC. Also, the rigidity had been improved from OL-BLF to OL-PUF, while with the highest for OL-MCC with 74.2% crystallinity, endowing it as a reliable load-bearing agent. As for thermal analysis, OL-MCC had the most stable heat resistance in among the chemically-treated fibers. Therefore, olive MCC could act as a promising reinforcing agent to withstand harsh conditions for variety fields of composite applications.

The effect of incorporation Nano Cinnamon powder on the shear bond of the orthodontic composite (an in vitro study).

OBJECTIVES: One of the causes of dental caries that occurs due to orthodontic treatment is the lack of antibacterial properties in orthodontic adhesive. This study was designated to investigate the effect of orthodontic resin modified by incorporating Nano Cinnamon powder on the shear bond strength of orthodontic brackets. MATERIALS AND METHODS: Heliosit Orthodontic Resin, a photo-activated light cure resin was modified by the addition of Cinnamon in the form of Nano particle powder. Twenty uniform disks were made, 5 as a control and 5 for each concentration of the 1%, 3% and 5% wt/wt Cinnamon modified resin. Their antimicrobial activity against Streptococcus Mutans was tested using the disk diffusion method. Then, the most effective concentration of the modified resin was used to bond metal orthodontic brackets to human extracted premolars. The universal testing machine was used to record the shear bond strength of the control and the modified resin. Also, the adhesive remnant index was measured. RESULTS: Disc diffusion method showed that the 3% wt/wt Cinnamon powder modified resin was more effective than 1% with a larger bacterial inhibition zone. Shear bond strengths of the control were 8.50 MPa and 7.20 MPa for the 3% Cinnamon modified resin with no significant difference between them. Also, no significant difference was recorded in the adhesive remnant index scores between the control and the modified resin groups. CONCLUSION: Findings of this study revealed that the incorporation of 3% Cinnamon Nano particles in orthodontic resin produced an antibacterial effect against Streptococcus mutans without compromising the shear bond strength. CLINICAL RELEVANCE: Incorporation of Cinnamon Nano particles in orthodontic resin may reduce caries formation around brackets during treatment course.

Antiviral activities of Cholistani plants against common poultry viruses.

Herbal medicines are becoming more popular and acceptable day by day due to their effectiveness, limited side effects, and cost-effectiveness. Cholistani plants are reported as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant, and anticancer agents. The current study has evaluated antiviral potential of selected Cholistani plants. The whole plants were collected, ground and used in extract formation with n-hexane, ethyl acetate and n-butanol. All the extracts were concentrated by using a rotary evaporator and concentrate was finally dissolved in an appropriate vol of the same solvent. All of the extracts were tested for their antiviral potential by using 9-11 days old chick embryonated eggs. Each extract was tested against the Avian Influenza virus H9N2 strain (AIV), New Castle Disease virus Lasoota strain (NDV), Infectious bronchitis virus (IBV) and an Infectious bursal disease virus (IBDV). Hemagglutination test (HA) and Indirect Hemagglutination (IHA) tests were performed for different viruses. The overall order of the antiviral potential of Cholistani plants against viruses was NDV>IBV>IBDV>AIV. In terms of antiviral activity from extracts, the order of activity was n-butanol>ethyl acetate>n-hexane. The medicinal plants Achyranthes aspera, Neuroda procumbens, Panicum antidotale, Ochthochloa compressa and Suaeda fruticose were very effective against all four poultry viruses through their extracts. The low IC50 values of these extracts confirm the high antiviral potential against these viruses. It is worth to mention that Achyranthes aspera was found positive against IBDV through all its extracts which overcome the problem of unavailability of any known drug against IBDV. In short, the study proved that Cholistani plants are rich source of antiviral agent and their extracts can be used as good source of antiviral drugs both in crude and in purified form.

Antiviral activities of Cholistani plants against common poultry viruses

@#Herbal medicines are becoming more popular and acceptable day by day due to their effectiveness, limited side effects, and cost-effectiveness. Cholistani plants are reported as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant, and anticancer agents. The current study has evaluated antiviral potential of selected Cholistani plants. The whole plants were collected, ground and used in extract formation with n-hexane, ethyl acetate and n-butanol. All the extracts were concentrated by using a rotary evaporator and concentrate was finally dissolved in an appropriate vol of the same solvent. All of the extracts were tested for their antiviral potential by using 9-11 days old chick embryonated eggs. Each extract was tested against the Avian Influenza virus H9N2 strain (AIV), New Castle Disease virus Lasoota strain (NDV), Infectious bronchitis virus (IBV) and an Infectious bursal disease virus (IBDV). Hemagglutination test (HA) and Indirect Hemagglutination (IHA) tests were performed for different viruses. The overall order of the antiviral potential of Cholistani plants against viruses was NDV>IBV>IBDV>AIV. In terms of antiviral activity from extracts, the order of activity was n-butanol>ethyl acetate>n-hexane. The medicinal plants Achyranthes aspera, Neuroda procumbens, Panicum antidotale, Ochthochloa compressa and Suaeda fruticose were very effective against all four poultry viruses through their extracts. The low IC50 values of these extracts confirm the high antiviral potential against these viruses. It is worth to mention that Achyranthes aspera was found positive against IBDV through all its extracts which overcome the problem of unavailability of any known drug against IBDV. In short, the study proved that Cholistani plants are rich source of antiviral agent and their extracts can be used as good source of antiviral drugs both in crude and in purified form.

The effect of different mouth washes and text messages reminder in the oral health of orthodontic patients

Aim: The aim of this study was to investigate the effect of different mouth washes and to study the effect of text message reminder on the oral health status of orthodontic patients treated with fixed appliances. Methods: This study was a clinical trial with pre-test and post-test control group design, conducted on 24 patients undergoing fixed orthodontic treatment aged 15-30 years. The patients randomly divided into two groups; one received a weekly telephone text massage reminder for the instructions of brushing and rinsing while the second group did not receive any messages. Ortho-plaque index and gingival index were used to evaluate the patient's oral hygiene status. The reading indices after brushing only were regarded as a control, then the patients were instructed to use two mouth washes, one containing sodium fluoride with cetylpyridinium chloride, the second containing chlorhexidine digluconate with cetylpyridinium chloride and Aloe Vera. Each one was used routinely for 4 weeks with the same amount and method. The washout period between the two types mouth washes was 4 weeks with a standardized toothbrush and paste. Results: Ortho-plaque index values decreased over the time between first, second and third visits. However, this index raised up at the fourth visit and again dropdown at the fifth visit. Similar results were detected for gingival index. Also, significant interaction between messages and the assessments was recorded for gingival index with a significant difference between the group of text messages and without text messages group (p<0.05). Conclusions: The results of this study showed that the combination of sodium fluoride with cetylpyridinium chloride was more potent for plaque control, while chlorhexidine digluconate with cetylpyridinium chloride and Aloe Vera combination showed a better gingival improvement. Moreover, the text messages reminder could enhance, but not replace direct oral hygiene instruction in orthodontic patients

Helicity conservation in V-point diffraction.

In singular beams, topological charge is conserved during diffraction. Like scalar field diffraction, in vector field diffraction also, there are conserved quantities. A diffracting V-point disintegrates into a number of C-points of the same polarity in which the polarization singularity index is conserved. In this Letter, we show for the first time, to the best of our knowledge, that apart from the index, the helicity (handedness) is also conserved in V-point diffraction. Since V-point is devoid of any handedness, the helicity conservation entails that there is an equal number of opposite handed C-points in the diffracted field, which are interestingly also found to be orthogonal pairs. Further, coexistence of C-points of opposite handedness in the diffraction demands the presence of L-line, which is also shown. We experimentally demonstrate these by studying the diffraction phenomenon through two different types of apertures.

Characterization of natural fiber obtained from different parts of date palm tree (Phoenix dactylifera L.).

The current study is motivated by the strict environmental regulations regarding the utilization and consumption of ecofriendly materials. In this context, the aim of this study has been to prepare and characterize different date palm tree (Phoenix dactylifera L.) fibers processed through the conventional water retting method. The chemical, elemental, crystallinity, thermal and morphological characterization of trunk (DPTRF), leaf stalk (DPLST), sheath or leaf sheath (DPLSH) and fruit bunch stalk (DPFBS) fibers was carried out. Chemical analysis revealed that the four types of date palm fibers display noteworthy differences in the content of cellulose, hemicellulose and lignin. Also, the amount of calcium is relatively high in all the date palm fibers; besides this, DPTRF exhibited 69.2% crystallinity, which is lower than that of DPLSH with 72.4% crystallinity. Moreover, DPLST and DPFBS fibers are more thermally stable (higher thermal degradation temperature) than DPTRF and DPLSH samples. Morphological analysis revealed that the fracture surface of DPFBS was relatively rougher, which would probably lead to increased bonding strength with polymers in composites. Overall, we conclude that DPFBS would be promising alternative sustainable and biomass material for the isolation of respective cellulose nanofibers and cellulose nanocrystals as potential reinforcement in polymer composites.