An inventory of online reptile images.

No central online repository exists for the collection of animal images; hence it remains unclear how extensively species have been illustrated in the published literature or online. Here we compiled a list of more than 8000 reptile species (out of 11,341) that have photos in one of six popular online repositories, namely iNaturalist (6,349 species), the Reptile Database (5,144), Flickr (4,386), CalPhotos (3,071), Wikimedia (2,952), and Herpmapper (2,571). These sites have compiled over one million reptile photos, with some species represented by tens of thousands of images. Despite the number of images, many species have only one or a few images. This suggests that a considerable fraction of morphological and geographic variation is under documented or difficult to access. We highlight prominent gaps in amphisbaenians, lizards, and snakes, with geographic hotspots for species without images in Central Africa, Pacific Islands, and the Andes Mountains. We present a list of ~3,000 species without photos in any of the six databases and ask the community to fill the gaps by depositing images on one of these sites (preferably with minimal copyright restrictions).

An inventory of online reptile images

No central online repository exists for the collection of animal images; hence it remains unclear how extensively species have been illustrated in the published literature or online. Here we compiled a list of more than 8000 reptile species (out of 11,341) that have photos in one of six popular online repositories, namely iNaturalist (6,349 species), the Reptile Database (5,144), Flickr (4,386), CalPhotos (3,071), Wikimedia (2,952), and Herpmapper (2,571). These sites have compiled over one million reptile photos, with some species represented by tens of thousands of images. Despite the number of images, many species have only one or a few images. This suggests that a considerable fraction of morphological and geographic variation is under documented or difficult to access. We highlight prominent gaps in amphisbaenians, lizards, and snakes, with geographic hotspots for species without images in Central Africa, Pacific Islands, and the Andes Mountains. We present a list of ~3,000 species without photos in any of the six databases and ask the community to fill the gaps by depositing images on one of these sites (preferably with minimal copyright restrictions).

Habitat, species richness and hantaviruses of sigmodontine rodents within the Interior Atlantic Forest, Paraguay.

Four of the nine sigmodontine tribes have species that serve as reservoirs of rodent-borne hantaviruses (RBO-HV), few have been studied in any depth. Several viruses have been associated with human cases of hantavirus pulmonary syndrome often through peridomestic exposure. Jabora (JABV) and Juquitiba (JUQV), harbored by Akodon montensis and Oligoryzomys nigripes, respectively, are endemic and sympatric in the Reserva Natural de Bosque Mbaracayú (RNBM), Paraguay, a protected area of the Interior Atlantic Forest. Rodent communities were surveyed along a 30 km stretch of the RNBM in eight vegetation classifications (Low, High, Bamboo, Riparian and Liana Forests, Bamboo Understory, Cerrado, and Meadow/Grasslands). We collected 417 rodents from which 11 species were identified; Akodon montensis was the predominant species (72%; 95%CI: 64.7%-76.3%), followed by Hylaeamys megacephalus (15% (11.2%-18.2%)) and Oligoryzomys nigripes (9% (6.6%-12.4%)). We examined the statistical associations among habitat (vegetation class) type, rodent species diversity, population structure (age, sex, and weight), and prevalence of RBO-HV antibody and/or viral RNA (Ab/RNA) or characteristic Leishmania tail lesions. Ab/RNA positive rodents were not observed in Cerrado and Low Forest. A. montensis had an overall Ab/RNA prevalence of 7.7% (4.9%-11.3%) and O. nigripes had an overall prevalence of 8.6% (1.8%-23.1%). For A. montensis, the odds of being Ab/RNA positive in High Forest was 3.73 times of the other habitats combined. There was no significant difference among age classes in the proportion of Ab/RNA positive rodents overall (p = 0.66), however, all 11 RNA-positive individuals were adult. Sex and habitat had independent prognostic value for hantaviral Ab/RNA in the study population; age, presence of tail scar/lesion (19% of the rodents) and weight did not. Adjusting for habitat, female rodents had less risk of becoming infected. Importantly, these data suggest habitat preferences of two sympatric rodent reservoirs for two endemic hantaviruses and the importance of including habitat in models of species diversity and habitat fragmentation.

Description of a new species of Haemogamasus (Mesostigmata, Laelapidae, Haemogamasinae) from Chubut, Río Negro and Neuquén Provinces, Argentina.

A new species of Haemogamasus mites is described from Chubut, Neuquén and Río Negro Provinces, Argentina. It was collected primarily from rodents of the genus Abrothrix (65 of 77 collections): Abrothrix longipilis (63), Abrothrix olivaceus olivaceus (1) and Abrothrix olivaceus xanthorhinus (1). Additional collections were made from Geoxus valdivianus (5) and Loxdomtomys micropus (5). Possibly incidental or contaminate collections were recorded from Oligoryzomys longicaudatus (1) and Dromiciops gliroides (1). Most collections came from Río Negro Province (36), with 22 from Neuquén and 19 from Chubut. An identification key is provided to distinguish ♀s of this new species from ♀s of species from the western hemisphere, based on morphological characters.

Description of a new species of Ectinorus (E. spiculatus) (Siphonaptera, Rhopalopsyllidae) from Argentina and a review of the subgenus Ichyonus Smit, 1987.

A new species, Ectinorus spiculatus, is described from Phyllotis xanthopygus (Waterhouse) and Akodon iniscatus Thomas from Neuquén Province, Argentina. Habitat characteristics are presented for the type locality. A change in status of four additional subspecies of the Ectinorus subgenus Ichyonus Smit is provided. Ectinorus onychius onychius Jordan and Rothschild, Ectinorus onychius deplexus Smit and Ectinorus onychius angularis Smit & Rosický were elevated to specific status. Ectinorus (Ichyonus) onychius fueginus was relegated as a junior synonym of the nominate species. Phyllotis xanthopygus, Abrothrix olivaceus xanthorhinus, Loxodontomys micropus Waterhouse, and Euneomys chinchilloides (Waterhouse) are new host records for Ectinorus onychius. A key to the three species of Ichyonus is included.

Modularity in the mammalian dentition: mice and monkeys share a common dental genetic architecture.

The concept of modularity provides a useful tool for exploring the relationship between genotype and phenotype. Here, we use quantitative genetics to identify modularity within the mammalian dentition, connecting the genetics of organogenesis to the genetics of population-level variation for a phenotype well represented in the fossil record. We estimated the correlations between dental traits owing to the shared additive effects of genes (pleiotropy) and compared the pleiotropic relationships among homologous traits in two evolutionary distant taxa-mice and baboons. We find that in both mice and baboons, who shared a common ancestor >65 Ma, incisor size variation is genetically independent of molar size variation. Furthermore, baboon premolars show independent genetic variation from incisors, suggesting that a modular genetic architecture separates incisors from these posterior teeth as well. Such genetic independence between modules provides an explanation for the extensive diversity of incisor size variation seen throughout mammalian evolution-variation uncorrelated with equivalent levels of postcanine tooth size variation. The modularity identified here is supported by the odontogenic homeobox code proposed for the patterning of the rodent dentition. The baboon postcanine pattern of incomplete pleiotropy is also consistent with predictions from the morphogenetic field model.

No trade-off between seed size and number in the valley oak Quercus lobata.

We examined the relationship between acorn mass and number in valley oaks (Quercus lobata) over 4 years in central coastal California. Despite considerable variation in acorn size among both trees and years, trees produced acorns of the same size relative to other trees in different years. Across years, the relationship between acorn mass and acorn crop size was generally positive, even after controlling for environmental conditions and differences in individual tree size and quality. Life-history trade-offs in valley oaks are primarily between current and future reproduction and indirectly between concurrent growth and reproduction, not between seed size and number, and are probably related to this species' mast-seeding behavior. Phenotypic trade-offs in long-lived plants such as oaks exhibit complex patterns of life-history covariation and deserve greater attention, both theoretically and empirically.

SPECIATION BY POLYPLOIDY IN TREEFROGS: MULTIPLE ORIGINS OF THE TETRAPLOID, HYLA VERSICOLOR.

Speciation by polyploidy is rare in animals, yet, in vertebrates, there is a disproportionate concentration of polyploid species in anuran amphibians. Sequences from the cytochrome b gene of the mitochondrial DNA (mtDNA) were used to determine phylogenetic relationships among 37 populations of the diploid-tetraploid species pair of gray treefrogs, Hyla chrysoscelis and Hyla versicolor. The diploid species, H. chrysoscelis, consists of an eastern and a western lineage that have 2.3% sequence divergence between them. The tetraploid species, H. versicolor, had at least three separate, independent origins. Two of the tetraploid lineages are more closely related to one or the other of the diploid lineages (0.18%-1.4% sequence divergence) than they are to each other (1.9%-3.4% sequence divergence). The maternal ancestor of the third tetraploid lineage is unknown. The phylogenetic relationships between the two species and among lineages within each species support the hypothesis of multiple origins of the tetraploid lineages.

ABRUPT CLINE FOR SEX CHROMOSOMES IN A HYBRID ZONE BETWEEN TWO SPECIES OF MICE.

We compared the patterns of movement of sex chromosomal and autosomal loci along a 160 km transect across a zone of hybridization between M. domesticus and M. musculus in southern Germany and western Austria using seven genetic markers. These included one Y-specific DNA sequence (YB10), two X-specific loci (DXWas68 and DXWas31), and four autosomal isozyme loci (Es-10, Es-1, Mpi-1, and Np-1). Random effects logistic regression analysis enabled us to examine the relationship between M. domesticus allele frequency and geographic distance from the western edge of the hybrid zone and allowed statistical evaluation of differences in cline midpoint and width among loci. More limited movement was observed for all three sex chromosomal markers across the zone compared with three of the four autosomal markers. If differential movement reflects fitness differences of specific alleles (or alleles at closely linked loci) on a hybrid background, then alleles that move to a limited extent across a hybrid zone may contribute to hybrid breakdown between two species. The limited flow of both X- and Y-specific alleles suggest that sex chromosomes have played an important role in Mus speciation.

FURTHER GENETIC ANALYSES OF A HYBRID ZONE BETWEEN LEOPARD FROGS (RANA PIPIENS COMPLEX) IN CENTRAL TEXAS.

Additional animals were collected from a previously described hybrid zone between two species of leopard frogs. The material now includes 1,054 specimens collected over a five-year period. The new and the original data were reanalyzed using two multilocus techniques: an analysis of linkage disequilibrium (Hill, 1975); and an analysis of the variance of the distribution of individual heterozygosities (Brown et al., 1980). An analysis of cline shape (Barton and Hewitt, 1983) also was performed. The zone appears to be stable in position. There are major year-to-year changes in allele frequencies, but these do not show concordant patterns between localities. This is probably the result of local stochastic factors. The shapes and position of clines at five loci are similar. Multilocus analysis shows the greatest disequilibrium values in the center of the zone. The ratio of observed to expected variance of individual heterozygosity is also largest in the central localities. Analysis of a single cohort during a three-month period that included both the larval and post-metamorphic stages shows a strong shift in genotypes. The froglet population had more animals with parental genotypes and fewer hybrid combinations. This probably is a result of differential mortality due to developmental incompatibilities when hybrids begin to metamorphose. We compare the usefulness of the two multilocus analytical techniques and make recommendations about collecting procedures for future studies of hybrid zones.