Elucidating the Anticancer Mechanisms of Cinnamoyl Sulfonamide Hydroxamate: Insights From DNA Content Analysis and Gene Expression Profiling in Squamous Cell Carcinoma.

BACKGROUND: Oral cancer is a major public health concern worldwide, with oral squamous cell carcinoma (OSCC) being one of its most common subtypes. Despite advances in diagnosis and management of this disease, there remains a need to develop new therapeutic approaches for better outcomes. OBJECTIVE: This study aimed to investigate the molecular mechanisms through which cinnamoyl sulfonamide hydroxamate derivatives exert their anticancer effects on OSCC. MATERIALS AND METHODS: The derivatives were synthesized via multi-step processes and then characterized at the molecular level. Flow cytometry assay for DNA content and cell cycle distribution, anisidine/toluidine double staining for apoptosis detection, as well as reverse transcription polymerase chain reaction (RT-PCR) gene expression analysis, were performed on OSCC cell lines exposed to cinnamoyl sulfonamide hydroxamate derivatives. RESULTS: Flow cytometry unveiled remarkable changes in the distribution of cells throughout the OSCC cell line upon treatment with cinnamoyl sulfonamide hydroxamate derivatives. Consequently, it led to a noticeable decrease in cells at the G0/G1 phase, together with an increase at the S phase, thereby indicating a retardation at various points of the cycle. In addition, apoptotic morphological alterations have been observed by anisidine/toluidine double staining after some treatments with the compounds. RT-PCR analysis showed a marked increase in p21 gene expression levels, further supporting the compounds' ability to induce cell cycle arrest and apoptosis. CONCLUSION: The research highlighted the potential of cinnamoyl sulfonamide hydroxamate derivatives as candidates for oral cancer, particularly OSCC treatment, shedding light on their operation at the molecular level and paving the way for the development of targeted therapies that could aid in the cure of oral cancer.

Biochar impacts on carbon dioxide, methane emission, and cadmium accumulation in rice from Cd-contaminated soils; A meta-analysis.

Climate change and cadmium (Cd) contamination pose severe threats to rice production and food security. Biochar (BC) has emerged as a promising soil amendment for mitigating these challenges. To investigate the BC effects on paddy soil upon GHG emissions, Cd bioavailability, and its accumulation, a meta-analysis of published data from 2000 to 2023 was performed. Data Manager 5.3 and GetData plot Digitizer software were used to obtain and process the data for selected parameters. Our results showed a significant increase of 18% in soil pH with sewage sludge BC application, while 9% increase in soil organic carbon (SOC) using bamboo chips BC. There was a significant reduction in soil bulk density (8%), but no significant effects were observed for soil porosity, except for wheat straw BC which reduced the soil porosity by 6%. Sewage sludge and bamboo chips BC significantly reduced carbon dioxide (CO2) by 7-8% while municipal biowaste reduced methane (CH4) emissions by 2%. In the case of heavy metals, sunflower seedshells-derived materials and rice husk BC significantly reduced the bioavailable Cd in paddy soils by 24% and 12%, respectively. Cd uptake by rice roots was lowered considerably by the addition of kitchen waste (22%), peanut hulls (21%), and corn cob (15%) based BC. Similarly, cotton sticks, kitchen waste, peanut hulls, and rice husk BC restricted Cd translocation from rice roots to shoots by 22%, 27%, 20%, and 19%, respectively, while sawdust and rice husk-based BC were effective for reducing Cd accumulation in rice grains by 25% and 13%. Regarding rice yield, cotton sticks-based BC significantly increased the yield by 37% in Cd-contaminated paddy soil. The meta-analysis demonstrated that BC is an effective and multi-pronged strategy for sustainable and resilient rice cultivation by lowering greenhouse gas emissions and Cd accumulation while improving yields under the increasing threat of climate change.

Spatiotemporal investigation of near-surface CH4 and factors influencing CH4 over South, East, and Southeast Asia.

Methane (CH4) is the second most abundant greenhouse gas after CO2, which plays the most important role in global and regional climate change. To explore the long-term spatiotemporal variations of near-surface CH4, datasets were extracted from Greenhouse gases Observing SATellite (GOSAT), and the Copernicus Atmospheric Monitoring Service (CAMS) reanalyzed datasets from June 2009 to September 2020 over South, East, and Southeast Asia. The accuracy of near-surface CH4 from GOSAT and CAMS was verified against surface observatory stations available in the study region to confirm both dataset applicability and results showed significant correlations. Temporal plots revealed continuous inflation in the near-surface CH4 with a significant seasonal and monthly variation in the study region. To explore the factors affecting near-surface CH4 distribution, near-surface CH4 relationship with anthropogenic emission, NDVI data, wind speed, temperature, precipitation, soil moisture, and relative humidity were investigated. The results showed a significant contribution of anthropogenic emissions with near-surface CH4. Regression and correlation analysis showed a significant positive correlation between NDVI data and near-surface CH4 from GOSAT and CAMS, while a significant negative correlation was found between wind and near-surface CH4. In the case of temperature, soil moisture, and near-surface CH4 from GOSAT and CAMS over high CH4 regions of the study area showed a significant positive correlation. However significant negative correlations were found between precipitation and relative humidity with GOSAT and CAMS datasets over high CH4 regions in South, East, and Southeast Asia. Moreover, these climatic factors showed no significant correlation within the low near-surface CH4 areas in our study region. Our study results showed that anthropogenic emissions, NDVI data, wind speed, temperature, precipitation, soil moisture, and humidity could significantly affect the near-surface CH4 over South, East, and Southeast Asia.

Exploration of linear and third-order nonlinear optical properties for donor-π-linker-acceptor chromophores derived from ATT-2 based non-fullerene molecule.

In the current study, seven non-fullerene compounds abbreviated as ATTD2-ATTD8 were designed through structural tailoring and their nonlinear optical (NLO) properties were reported. The objective of this study was to explore the potential for newly configured D-π-A type non-fullerene-based compounds. Quantum chemical methods were adopted and revealed the molecules as highly efficient materials with favorable NLO characteristics for use in optoelectronic devices. The M06 functional along with the 6-311G(d,p) basis set in chloroform solvent were utilized for the natural bonding orbital (NBO) analysis, absorption spectra and computational assessments of frontier molecular orbitals (FMOs), global reactivity descriptors (GRPs), transition density matrix (TDM) and nonlinear optical properties (NLO) for ATTR1 and ATTD2-ATTD8. The HOMO-LUMO energy gap was significantly reduced in all the designed moieties compared to the reference compound in the following decreasing order: ATTR1 > ATTD8 > ATTD4 > ATTD5 > ATTD2 > ATTD7 > ATTD6 > ATTD3. All of the designed molecules (ATTD2-ATTD8) showed good NLO response. Global reactivity parameters were found to be closely associated with the band gap between the HOMO and LUMO orbitals, and the compound with the smallest energy gap, ATTD3, exhibited a lower hardness value of 1.754 eV and higher softness value of 0.570 eV with outstanding NLO response. For the reference compound and ATTD2-ATTD8 derivatives, attributes like dipole moment (µtot), average polarizability 〈α〉, first hyperpolarizability (ßtot), and second hyperpolarizability γtot were calculated. Out of all the derivatives, ATTD3 revealed the highest amplitude with a ßtot of 8.23 × 10-27 esu, which was consistent with the reduced band gap (1.754 eV) and suggested it was the best possibility for NLO materials in the future.

Exploration of nonlinear optical enhancement in acceptor-π-donor indacenodithiophene based derivatives via structural variations: a DFT approach.

Herein, a series of indacenodithiophene-based derivatives (TNPD1-TNPD6) were designed having D-π-A architecture via end capped acceptor modulation of a reference molecule (TNPR) to investigate nonlinear optical (NLO) behavior. Quantum chemical calculations were accomplished to examine electronic, structural and optical properties utilizing a density functional theory (DFT) approach at M06 functional with 6-311G(d,p) basis set. For this, natural bond orbitals (NBOs), density of states (DOS), frontier molecular orbitals (FMOs), transition density matrix (TDM) and non-linear optical (NLO) analyses were performed for TNPR and TNPD1-TNPD6. The structural modifications revealed a significant electronic contribution in tuning the HOMOs and LUMOs of the derivatives with lowered energy gaps and wider absorption spectra. FMOs findings revealed that compound TNPD5 was found with the lowest energy gap (1.692 eV) and with the highest softness (0.591 eV-1) among the derivatives. Furthermore, a UV-Vis study also disclosed that maximum absorption (λmax = 852.242 nm) was exhibited by TNPD5 in chloroform solvent. All the derivatives exhibited significant NLO results; in particular, TNPD5 showed the highest first hyper-polarizability (ßtot = 4.653 × 10-27 esu) and second hyper-polarizability (γtot = 9.472 × 10-32 esu). These DFT findings revealed that the end-capped substituents play a key role in enhancing the NLO response of these push-pull chromophores and the studied derivatives can be utilized as efficient NLO materials.

Hydrothermal synthesis of V2O5/TiO2 decorated graphitic carbon nitride nanocomposite for biomolecule oxidation inhibition and dye degradation applications.

Oxides of vanadium, titanium and graphitic carbon nitride (g-C3N4) are well known for their catalytic activities. In order to achieve synergic catalytic effects, a novel nanocomposite (NC) i.e. V2O5/TiO2/g-C3N4 has been synthesized by a very simple, ecofriendly and nonhazardous hydrothermal method. The fabricated NC was characterized employing UV-Visible, FTIR, SEM, and XRD techniques. UV-Visible and FTIR analysis indicated the formation of the nanocomposite and XRD analysis confirmed the association of V2O5 and TiO2 with g-C3N4 in nanocomposite. SEM study indicated the hetero-structure of NC having size ranging from 50 to 80 nm and it was found having hexagonal crystallite structure. The synthesized nanocomposite exhibited excellent scavenging of free radicals DPPH● (91%) and ABTS●+ (64%) that are responsible for the oxidation of biomolecules. Therefore, NC can be claimed having biomolecule oxidation protective potential. In addition, photocatalytic ability for the degradation of methylene blue (MB) and methyl orange (MO) was also achieved up to 94% and 89% respectively. The synthesized novel nanocomposite exhibited excellent potential to remove free radicals and dyes from aqueous medium which can be further used for the environmental remediation.

Performance evaluation of phosphonium based deep eutectic solvents coated cerium oxide nanoparticles for CO2 capture.

The critical challenge being faced by our current modern society on a global scale is to reduce the surging effects of climate change and global warming, being caused by anthropogenic emissions of CO2 in the environment. Present study reports the surface driven adsorption potential of deep eutectic solvents (DESs) surface functionalized cerium oxide nanoparticles (CeNPs) for low pressure CO2 separation. The phosphonium based DESs were prepared using tetra butyl phosphoniumbromide as hydrogen bond acceptor (HBA) and 6 acids as hydrogen bond donors (HBDs). The as-developed DESs were characterized and employed for the surface functionalization of CeNPs with their subsequent utilization in adsorption-based CO2 adsorption. The synthesis of as-prepared DESs was confirmed through FTIR measurements and absence of precipitates, revealed through visual observations. It was found that DES6 surface functionalized CeNPs demonstrated 27% higher adsorption performance for CO2 capturing. On the contrary, DES3 coated CeNPs exhibited the least adsorption progress for CO2 separation. The higher adsorption performance associated with DES6 coated CeNPs was due to enhanced surface affinity with CO2 molecules that must have facilitated the mass transport characteristics and resulted an enhancement in CO2 adsorption performance. Carboxylic groups could have generated an electric field inside the pores to attract more polarizable adsorbates including CO2, are responsible for the relatively high values of CO2 adsorption. The quadruple movement of the CO2 molecules with the electron-deficient and pluralizable nature led to the enhancement of the interactive forces between the CO2 molecules and the CeNPs decorated with the carboxylic group hydrogen bond donor rich DES. The current findings may disclose the new research horizons and theoretical guidance for reduction in the environmental effects associated with uncontrolled CO2 emission via employing DES surface coated potential CeNPs.

Spinach-derived boron-doped g-C3N4/TiO2 composites for efficient photo-degradation of methylene blue dye.

Green synthesis of nanoparticles can be beneficial due to their low toxicity, cost-effectiveness, and environment-friendliness. Its synthesis involves the use of eco-friendly and biodegradable materials such as plant extracts, natural products, and microorganisms to reduce the negative environmental impacts of traditional nanoparticle synthesis methods. Herein, Spinacia oleracea leaves are used as a boron source, and a visible light active photo-catalyst is produced. The effect of Co-Catalyst Boron in Graphitic carbon nitride based nanocomposites for methylene blue dye photo-degradation in water is examined. Titanium dioxide (TiO2) was activated by changing the hydrogen potential value while utilizing a typical orange dye as a sensitizer. The graphitic carbon nitride/TiO2 nanocomposites were synthesized through a hydrothermal technique. To improve their performance, Boron used as a co-catalyst and B-doped g-C3N4/TiO2nanocomposites prepared through wet chemical co-percipitate mathod. UV-visible spectroscopy, SEM and FTIR spectroscopy were used to analyze the photocatalyst and boron-doped composites in detail. The photocatalytic performance of pristine photocatalyst CNTx (x = 2%,4%,6%,8%) and B-doped CNTx composites were examined for Methylene Blue degradation in the presence of a light source. The spectroscopy analysis showed that B-doped g-C3N4/TiO2 -8% nano-composites performed better than all other synthesized pristine catalysts and composites in this research. This research has demonstrated that B-doped g-C3N4/TiO2 composites can provide an ideal solution for treating polluted water using visible light as a source to activate these photocatalysts.

Exogenously applied melatonin enhanced the tolerance of Brassica napus against cobalt toxicity by modulating antioxidant defense, osmotic adjustment, and expression of stress response genes.

The excessive accumulation of cobalt (Co) in plant tissues severely impairs plant growth that ultimately reduces the yield. However, melatonin (MT) has been known to mediate the abiotic stress tolerance in plants. The present study aimed at investigating the protective mechanisms of exogenously applied MT (0, 50 and 100 µM) under Co (0, 100, 200 and 300 µM) stress by focusing on morpho-physiological, biochemical and cellular characterizations of Brassica napus plants. Cobalt (300 µM) alone treatment drastically inhibited the stomatal conductance, plant height (45%), leaf area (30%), free amino acid (139%), relative electrolyte leakage (109%), and total soluble sugars (71%), compared with the control. However, the exogenous supply of MT notably minimized the oxidative damage, lipid peroxidation and maintained the membrane integrity under Co-toxicity by restricting the overproduction of ROS (H2O2 and O2•), and MDA in leaves and roots. Melatonin significantly enhanced the activities of ROS-scavenging antioxidant enzymes, secondary metabolism-related phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), stress-responsive genes (heat shock protein as HSP-90, methyl transferase as MT) and regulated the Co-transporters, especially in roots. These findings indicated that an exogenous supply of MT improve the plant morphology, photosynthetic apparatus, osmotic adjustments, and antioxidant defense systems by enhancing the Co-detoxification in B. napus plants.

Functionalized Fe3O4-based methyl methacrylate Pickering PolyHIPE composites costabilized by fluorinated block copolymer for oil/water separation.

High internal phase emulsion (HIPE) technology has been emerged as a prodigious source to create tailor-made porous structures. This type of emulsion contains significantly higher amount of water in it, which is only possible with special type of stabilizers. Most specifically, the monomers with sufficiently high solubility in water such as methyl methacrylate (MMA) make it more cumbersome to stabilize in the form of HIPE. Here we have reported the combination of stabilizers including fluorinated block copolymer Poly (2-dimethylamino)ethyl methacrylate-b-Poly(trifluoroethyl methacrylate) (PDMAEMA-b-PTFEMA) and humic acid modified iron-oxide (HA-Fe3O4) nanoparticles (NPs) to stabilize HIPE, which resulted in highly porous and interconnected products. Fluorinated block copolymers with inherent hydrophobic nature along with iron oxide nanoparticles increased the water repellency of MMA based polymeric monoliths. Increasing the amount of stabilizer increased the porosity and BET specific surface area to 83.8% and 27 ± 0.8 µm, respectively. The prepared porous materials demonstrated hydrophobic characteristics while adsorbing oil from the surface of water up to 16 g/g. Moreover, the adsorbed oil from the prepared monolith was recovered by using simple centrifugation method without damaging the structure. This research opens new avenues to prepare more useful oil and water separation materials such as membranes, pollutant adsorbers, and so on.

Mitigation of salinity stress in barley genotypes with variable salt tolerance by application of zinc oxide nanoparticles.

Salinity has become a major environmental concern of agricultural lands, impairing crop production. The current study aimed to examine the role of zinc oxide nanoparticles (ZnO NPs) in reducing the oxidative stress induced by salinity and the overall improvement in phytochemical properties in barley. A total of nine different barley genotypes were first subjected to salt (NaCl) stress in hydroponic conditions to determine the tolerance among the genotypes. The genotype Annora was found as most sensitive, and the most tolerant genotype was Awaran 02 under salinity stress. In another study, the most sensitive (Annora) and tolerant (Awaran 02) barley genotypes were grown in pots under salinity stress (100 mM). At the same time, half of the pots were provided with the soil application of ZnO NPs (100 mg kg-1), and the other half pots were foliar sprayed with ZnO NPs (100 mg L-1). Salinity stress reduced barley growth in both genotypes compared to control plants. However, greater reduction in barley growth was found in Annora (sensitive genotype) than in Awaran 02 (tolerant genotype). The exogenous application of ZnO NPs ameliorated salt stress and improved barley biomass, photosynthesis, and antioxidant enzyme activities by reducing oxidative damage caused by salt stress. However, this positive effect by ZnO NPs was observed more in Awaran 02 than in Annora genotype. Furthermore, the foliar application of ZnO NPs was more effective than the soil application of ZnO NPs. Findings of the present study revealed that exogenous application of ZnO NPs could be a promising approach to alleviate salt stress in barley genotypes with different levels of salinity tolerance.

Remediation techniques for elimination of heavy metal pollutants from soil: A review.

Contaminated soil containing toxic metals and metalloids is found everywhere globally. As a consequence of adsorption and precipitation reactions, metals are comparatively immobile in subsurface systems. Hence remediation techniques in such contaminated sites have targeted the solid phase sources of metals such as sludges, debris, contaminated soils, or wastes. Over the last three decades, the accumulation of these toxic substances inside the soil has increased dramatically, putting the ecosystem and human health at risk. Pollution of heavy metal have posed severe impacts on human, and it affects the environment in different ways, resulting in industrial anger in many countries. Various procedures, including chemical, biological, physical, and integrated approaches, have been adopted to get rid of this type of pollution. Expenditure, timekeeping, planning challenges, and state-of-the-art gadget involvement are some drawbacks that need to be properly handled. Recently in situ metal immobilization, plant restoration, and biological methods have changed the dynamics and are considered the best solution for removing metals from soil. This review paper critically evaluates and analyzes the numerous approaches for preparing heavy metal-free soil by adopting different soil remediation methods.

Comparative study of ozonation and ozonation catalyzed by Fe-loaded biochar as catalyst to remove methylene blue from aqueous solution.

Ozone-based processes gained much attention in recent years. However, due to low oxidative stability and utilization rate, single ozonation process (SOP) is insufficient for complete mineralization of pollutants. As a result, the single ozonation process is performed in the presence of a catalyst, a process known as catalytic ozonation process (COP). A promising catalyst (Fe/BC) was prepared by impregnating iron on biochar surface to remove methylene blue from aqueous solution via heterogeneous catalytic ozonation process (HCOP). The prepared Fe/BC features were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and Brunauer-Emmett-Teller method (BET) before and after HCOP. Furthermore, the effect of various operating parameters such as ozone dose, catalyst dose, initial dye concentration, initial pH on the efficiency of SOP and HCOP were compared. In comparison to single ozonation process, the experimental study found that heterogeneous catalytic ozonation process has the highest efficiency. At pH 7.0, approximately 76% of methylene blue is removed during single ozonation process in 60 min. Heterogeneous catalytic ozonation process showed 95% methylene blue elimination from aqueous solution. The efficiency of heterogeneous catalytic ozonation process was decreased by 52% in the presence of hydroxyl radical (●OH) scavenger, indicating that hydroxyl is the major oxidant during heterogeneous catalytic ozonation process for the removal of methylene blue from aqueous solution. Fe/BC catalyst appears to have a lot of industrial promise, as well as the ability to remove methylene blue from aqueous solution via heterogeneous catalytic ozonation process.

Template-based textural modifications of polymeric graphitic carbon nitrides towards waste water treatment.

The composite materials based on graphitic carbon nitrides (g-C3N4) are remarkably better semiconductors, but the inherent photocatalytic performance in its generic synthesis form is not up to the mark. Eminence efforts have been made to improve its performance and photocatalytic efficiencies. Recently, extensive investigations have been performed to develop their texturally modified and highly porous structures to get around the big flaws of bulk g-C3N4. One significant disadvantage is the increase in the polycondensation while preparation at 550 °C results in g-C3N4 materials with restricted specific surface area (SSA) (<10 m2/g) and no textured pores. Textural modification has emerged as an efficient and progressive way to improve optical and electronic characteristics. The final texture and shape of CN are influenced by the precursor's interaction with the template. Researchers are interested in developing CN materials with high SSA and changeable textural properties (pore volume and pore size). Based on the literature review it is concluded that the soft templating approach is relatively simple, and straightforward to induce textural changes in the g-CN type materials. This review focused on improving the textural properties of bulk g-C3N4 via templating method, and the major advances in the modified g-C3N4 materials for the treatment of wastewater. The procedures and mechanisms of numerous approaches with varying morphologies are thoroughly explained.

Fe-zeolite catalyst for ozonation of pulp and paper wastewater for sustainable water resources.

The pulp and paper industry consumes enormous quality of freshwater, leading to wastewater. It must be treated to remove pollutants, particularly residual dyestuffs, before releasing them to water bodies to avoid adverse environmental effects. The traditional wastewater treatment methods used for the pulp and paper industry are less efficient in colour and chemical oxygen demand (COD) removal. The current study is aimed at developing a novel catalyst for the catalytic ozonation of pulp and paper wastewater with better colour and COD removal for sustainable resources of clean water. The proposed catalyst is impregnated by iron on natural zeolites. Various parameters such as catalyst dose, pH, ozone dose, initial COD concentration, and reaction time are studied and optimized. The performance was evaluated by comparing the results with the single ozonation process (SOP) and catalytic ozonation process (COP). The highest COD and colour reduction efficiencies have been achieved, i.e., 71%, and 88% at a natural pH of 6.8. The proposed process achieved higher COD and colour efficiencies than the single ozonation process and catalytic ozonation process using raw zeolites. The improvement in efficiencies are 23% and 29% for SOP and 17% and 19% for COP, respectively. Hence, the results proposed the sustainability and applicability of COP to treat paper and pulp sector effluent.

Amalgamation and Scrutinizing of Leucine Derivatives Schiff Bases Complexes as Antimicrobial Agent.

The enhanced applications of Schiff bases metal complexes of amino acid derivatives have captured the attention of researchers for the synthesis of leucine derivatives of Schiff bases metal complexes. Amino acids are considered to be essential part of food supplements as well as derivatives of Schiff bases in coordination chemistry due to their donor ability. The leucine derivatives Schiff bases ligand have been synthesized by condensation reaction between amine of leucine with aldehyde or ketone bearing molecules attached with them. These complexes were characterized by different spectroscopic tools in order to confirm their structural geometries. The structural geometries are considered to be very important in order to improve the antimicrobial potential of leucine derivative metal complexes. By taking into account the antimicrobial potential of titled compounds, a comprehensive review of leucine derivatives of Schiff bases metal complexes has been compiled.

Diet impacts on the biological aspects of pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae) under controlled laboratory conditions.

BACKGROUND: Pink bollworm, Pectinophora gossypiella (Lepidoptera: Gelechiidae) is a native pest of Asia and preferably invasion on cotton (Gossypium hirsutum L.) crop as a commendatory host plant. Commercially, G. hirsutum is known as white gold and is an important cash crop all over the globe. Limited studies were published to focus on certain dietary compositions against different cotton pests. Therefore, the present study was undertaken in the laboratory under controlled conditions (temperature: 27 ± 2°C and relative humidity: 60 ± 10%) to determine the impact of three different treatment diets (wheat germ meal, okra, and chickpea) on the biological aspects (lifetime, developmental period) of P. gossypiella. RESULTS: Results revealed that the shortest larval time of P. gossypiella was observed on the okra feed diet while the longest period was recorded on the wheat germ diet. Meanwhile, the pupation delay was noted on the wheat germ diet. The dietary influence was also observed on adult stages of female and male P. gossypiella (43.00 and 37.50 days respectively) and compared with a standard diet (56.50 and 52.50 days respectively). Furthermore, larval weighed more on the okra and chickpea diet followed by the wheat germ diet, whereas highest pupal weight was observed on the standard diet followed by the chickpea diet and okra diet. CONCLUSION: Developmental parameters were significantly variant across all treatment diets, whereas the higher significant difference was reported on the okra diet. Therefore, the existing data of this study offers fruitful interventions for the future as a modified diet for large-scale and rapid mass production of P. gossypiella larvae.

Techno-economic feasibility of waste biorefinery: Using slaughtering waste streams as starting material for biopolyester production.

The utilization of industrial waste streams as input materials for bio-mediated production processes constitutes a current R&D objective not only to reduce process costs at the input side but in parallel, to minimize hazardous environmental emissions. In this context, the EU-funded project ANIMPOL elaborated a process for the production of polyhydroxyalkanoate (PHA) biopolymers starting from diverse waste streams of the animal processing industry. This article provides a detailed economic analysis of PHA production from this waste biorefinery concept, encompassing the utilization of low-quality biodiesel, offal material and meat and bone meal (MBM). Techno-economic analysis reveals that PHA production cost varies from 1.41 €/kg to 1.64 €/kg when considering offal on the one hand as waste, or, on the other hand, accounting its market price, while calculating with fixed costs for the co-products biodiesel (0.97 €/L) and MBM (350 €/t), respectively. The effect of fluctuating market prices for offal materials, biodiesel, and MBM on the final PHA production cost as well as the investment payback time have been evaluated. Depending on the current market situation, the calculated investment payback time varies from 3.25 to 4.5years.