Assessing phytotoxicity and tolerance levels of ZnO nanoparticles on Raphanus sativus: implications for widespread adoptions.

The escalating release of zinc oxide nanoparticles (ZnO NPs) into the environment poses a substantial threat, potentially leading to increased concentrations of zinc (Zn) in the soil and subsequent phytotoxic effects. This study aimed to assess the effects of ZnO NPs on Raphanus sativus (R. sativus) concerning its tolerance levels, toxicity, and accumulation. ZnO NPs were synthesized by the wet chemical method and characterized by powder X-ray diffraction (PXRD), Fourier-transform infrared (FTIR) spectroscopy, ultraviolet-visible (UV-vis) spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The effect of ZnO NPs (70 nm) on R. sativus grown in coir was evaluated. The application of 1,000 mg/L of ZnO NPs resulted in a significant increase (p < 0.05) in soluble protein content, carbohydrates, chlorophyll a (Chl-a), chlorophyll b (Chl-b), total chlorophylls, carotenoids, and antioxidants by 24.7%, 58.5%, 38.0%, 42.2%, 39.9%, 11.2%, and 7.7%, respectively. Interestingly, this dose had no impact on the indole acetic acid (IAA) content. Conversely, the use of 2,000 mg/L of ZnO NPs in the same medium led to a significant reduction (p < 0.05) in soluble protein content by 23.1%, accompanied by a notable increase in IAA by 31.1%, indicating potential toxicity. The use of atomic absorption spectroscopy confirmed the internalization of zinc in seedlings, with a statistically significant increase (p < 0.05). In control plants without ZnO NPs, Zn concentration was 0.36 mg/g, while at the highest ZnO NPs tested dose of 10,000 mg/L, it significantly rose to 1.76 mg/g, causing leaf chlorosis and stunted seedling growth. This suggests potential health risks related to Zn toxicity for consumers. Given the adverse effects on R. sativus at concentrations above 1000 mg/L, caution is advised in the application and release of ZnO NPs, highlighting the importance of responsible practices to mitigate harm to plant life and consumer health. The study demonstrated the tolerance of R. sativus to high Zn levels, classifying it as a Zn-tolerant species.

Cellular localisation and quantification of Camptothecin in different plant parts of Nothapodytes nimmoniana (J. Graham) Mabberley of Sri Lankan origin.

INTRODUCTION: Nothapodytes nimmoniana (Icacinaceae) is a rich source of Camptothecin (CPT), an anti-cancer prodrug. Efficient extraction of CPT from various plant parts is crucial for better recovery of this pre-drug. OBJECTIVES: To investigate the distribution of CPT in plant parts and to compare the methods of extraction on CPT yield to evaluate how cellular localisation affects the efficiency of extraction methods. METHODS: Transverse sections of plant parts were observed under a ultraviolet (UV)-fluorescence microscope for the fluorescence that the CPT molecule emits when exposed to UV radiation. Dried plant parts were extracted using 90% methanol with ultrasonic assistance, hot ethanol (61% ethanol at 60°C), and chloroform-methanol (4:1, v/v). The CPT in plant parts were detected by thin-layer chromatography (TLC) and confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and high-performance liquid chromatography (HPLC). Quantification was carried out by HPLC. RESULTS: Blue fluorescence indicated a prominent accumulation of CPT in roots compared to leaf with petiole, twigs, and stembark. This accumulation was observed in upper and lower epidermis of the leaf, isolated strands of fibres in the phloem in the petiole, and groups of idioblast cells in the cortex. The ultrasonic-assisted extraction with 90% methanol showed the highest CPT yield in the root (1.91 ± 0.02 mg/g of dry weight), followed by stembark and the least in leaves [0.02 ± 0.01 mg/g (dry weight)] irrespective of the method of extraction. However, hot ethanol extraction gave the highest CPT yield for twig and leaf, indicating the necessity of tissue-specific extraction methods for better recovery of CPT.

Characterisation of Sri Lankan cassava mosaic virus and Indian cassava mosaic virus: evidence for acquisition of a DNA B component by a monopartite begomovirus.

Two bipartite begomoviruses, Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), have been isolated from mosaic-diseased cassava originating from central India and Sri Lanka, respectively. ICMV was transmitted with low efficiency from cassava to Nicotiana benthamiana by sap inoculation to give leaf curl symptoms. SLCMV was much more virulent in this host, producing severe stunting, leaf curl, and chlorosis. These symptoms were reproduced when their cloned genomic components (DNAs A and B) were introduced into N. benthamiana by either mechanical or Agrobacterium-mediated inoculation (agroinoculation). SLCMV is more closely related to ICMV (DNA A, 84%; DNA B, 94% nucleotide identity) than African cassava mosaic virus (ACMV) (DNA A, 74%; DNA B, 47% nucleotide identity). Sequence comparisons suggest that SLCMV DNA B originated from ICMV DNA B by a recombination event involving the SLCMV DNA A intergenic region. Pseudorecombinants produced by reassortment of the cloned components of ICMV and ACMV were not infectious in N. benthamiana, emphasising their status as distinct virus species. In contrast, a pseudorecombinant between ACMV DNA A and SLCMV DNA B was infectious. Consistent with these observations, iteron motifs located within the intergenic region that may be involved in the initiation of viral DNA replication are conserved between SLCMV and ACMV but not ICMV. When introduced into N. benthamiana by agroinoculation, SLCMV DNA A alone produced a severe upward leaf roll symptom, reminiscent of the phenotype associated with some monopartite begomoviruses. Furthermore, coinoculation of SLCMV DNA A and the satellite DNA beta associated with ageratum yellow vein virus (AYVV) produced severe downward leaf curl in N. glutinosa and yellow vein symptoms in Ageratum conyzoides, resembling the phenotypes associated with AYVV DNA A and DNA beta infection in these hosts. Thus, SLCMV DNA A has biological characteristics of a monopartite begomovirus, and the virus probably evolved by acquisition of a DNA B component from ICMV.