Effect of sustained-release antioxidant bolus on body condition score, blood parameters, uterine health, and some reproductive parameters in transition dairy cows under heat stress condition.

The transition period for dairy cows is stressful, and if this occurs during heat stress conditions, it will become more challenging for them. This study aimed to evaluate the effect of sustained-release bolus (Each bolus consisted of a mixture of mineral salts including copper sulfate (8 g), sodium selenite (0.17 g), manganese sulfate (3.9 g), zinc sulfate (2.4 g), and vitamin A (0.47 g) on body condition score (BCS) change, serum metabolites, uterine health, and some reproductive parameters in transition cows with moderate or high pre-calving BCS. Four experimental treatments were (1) moderate BCS without bolus consumption (MB-Bo, n = 35), (2) moderate BCS with bolus consumption (MB + Bo, n = 35), (3) high BCS without bolus consumption (HB-Bo, n = 35), and (4) high BCS with bolus consumption (HB + Bo, n = 35). Results showed that after calving, negative energy balance occurred in all experimental groups. However, cows with high BCS (HB-Bo and HB + Bo) had greater (P = 0.02) BCS change during the postpartum period (0-40 days). Bolus administration decreased white blood cells count 14 days after calving (P = 0.02). Cows with moderate BCS (MB-BO and MB + Bo) had higher (P < 0.01) red blood cell count than cows with high BCS (HB-Bo and HB + Bo) on 14 days after calving. The cows in MB + Bo group had higher (P < 0.05) serum glucose and albumin and lower (P < 0.01) non-esterified fatty acids and beta-hydroxybutyrate. Moreover, this group of cows had higher (P < 0.05) serum total antioxidant capacity, glutathione peroxidase and superoxide dismutase, and lower malondialdehyde (P = 0.03) than other groups. In this regard, the increase in antioxidant capacity with the consumption of blues caused the HB-Bo group to have more incidence of metritis (P = 0.08) and endometritis (P = 0.08). The HB-Bo group had about 12 days longer (P < 0.01) days open than MB + Bo group. It was concluded that consumption of slow-release bolus containing antioxidant elements had positive effect on the metabolic and reproductive status of high-producing dairy cows under heat stress condition.

Effect of sustained release bolus oral administration on body condition change, blood parameters, and uterine health in primiparous cows under heat stress.

The transition period is very stressful for primiparous cows due to their first calving experience and will be more challenging if it occurs under heat stress conditions. Heat stress reduces the feed intake of dairy cows. Therefore, it reduces the consumption of minerals and vitamins. Oral administration of boluses through the provision of mineral-vitamin compounds can reduce metabolic abnormalities after calving. The present study aimed to evaluate effect of sustained-release bolus on body condition score (BCS) change, serum metabolites, uterine health, and reproductive status in primiparous cows. Heifers were selected at the beginning of the close-up period (n = 60, BCS = 3.35 ± 0.12). There were 2 experimental treatments at the time of calving: (1) heifers without bolus oral administration (H - Bo, n = 30); (2) heifers with bolus oral administration (H + Bo, n = 30). The results showed that although the rate of BCS loss was lower in the group receiving bolus, the effect of bolus was not significant. The effect of bolus on blood level of glucose, non-esterified fatty acids (NEFA), and beta-hydroxybutyrate (BHBA) was not significant; however, the highest concentration of albumin (P = 0.05) was observed in the H + Bo group on day 42 after calving. The concentration of aspartate transaminase (AST) tended to increase (P = 0.06) on day 14 after calving and entire the study. Total antioxidant capacity (TAC) was affected (P < 0.05) by bolus throughout the period of study, and the highest (P < 0.05) concentration of glutathione peroxidase (GPX) and superoxide dismutase (SOD) was observed in H + Bo group on day 42 after calving. The H + Bo group had the lowest (P < 0.05) vaginal discharge score (VDS). In general, oral administration of the sustained-release bolus in heifers significantly affected the antioxidant factors and uterine health, as well as had positive effects on liver function, body condition, and reproduction status.

Short communication: Test of the relationship between prepartum milk leakage and blood calcium concentration in Holstein cows-An observational study.

Prepartum milk leakage happens in some pregnant dairy cows close to calving. It has been hypothesized that low blood Ca is a cause of this event. To investigate the possible reason(s) of milk leakage, 137 multiparous pregnant Holstein cows were enrolled in the experiment and categorized by the presence (72 cows; leak group) or lack (65 cows; control group) of milk leakage before calving. The concentrations of Ca and P and the length of the teat were measured for all cows. Data showed that Ca concentration was not different between cows in the leak group (7.90 mg/dL) and those in the control group (7.99 mg/dL). Moreover, neither P concentration (4.62 vs. 4.54 mg/dL) nor teat length (4.28 vs 4.10 cm) differed between leak and control groups. Milk yield was greater for the leak group (53.6 kg/d) compared with the control group (50.1 kg/d) through 4 mo in milk. The leakage did not affect the odds of postpartum disorders such as retained placenta, metritis, mastitis, displaced abomasum, or lameness occurrence. The current results show that hypocalcemia is not a reason for observed prepartum leakage and that cows in the leak group produced more milk in the subsequent lactation period.