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1.
Curr Biol ; 33(18): 3821-3834.e5, 2023 09 25.
Artículo en Inglés | MEDLINE | ID: mdl-37572663

RESUMEN

During central nervous system (CNS) development, a precisely patterned vasculature emerges to support CNS function. How neurons control angiogenesis is not well understood. Here, we show that the neuromodulator dopamine restricts vascular development in the retina via temporally limited production by an unexpected neuron subset. Our genetic and pharmacological experiments demonstrate that elevating dopamine levels inhibits tip-cell sprouting and vessel growth, whereas reducing dopamine production by all retina neurons increases growth. Dopamine production by canonical dopaminergic amacrine interneurons is dispensable for these events. Instead, we found that temporally restricted dopamine production by retinal ganglion cells (RGCs) modulates vascular development. RGCs produce dopamine precisely during angiogenic periods. Genetically limiting dopamine production by ganglion cells, but not amacrines, decreases angiogenesis. Conversely, elevating ganglion-cell-derived dopamine production inhibits early vessel growth. These vasculature outcomes occur downstream of vascular endothelial growth factor receptor (VEGFR) activation and Notch-Jagged1 signaling. Jagged1 is increased and subsequently inhibits Notch signaling when ganglion cell dopamine production is reduced. Our findings demonstrate that dopaminergic neural activity from a small neuron subset functions upstream of VEGFR to serve as developmental timing cue that regulates vessel growth.


Asunto(s)
Dopamina , Factor A de Crecimiento Endotelial Vascular , Dopamina/metabolismo , Factor A de Crecimiento Endotelial Vascular/metabolismo , Retina , Células Ganglionares de la Retina/metabolismo , Transducción de Señal
2.
Annu Rev Vis Sci ; 9: 177-199, 2023 09 15.
Artículo en Inglés | MEDLINE | ID: mdl-37196423

RESUMEN

Because the central nervous system is largely nonrenewing, neurons and their synapses must be maintained over the lifetime of an individual to ensure circuit function. Age is a dominant risk factor for neural diseases, and declines in nervous system function are a common feature of aging even in the absence of disease. These alterations extend to the visual system and, in particular, to the retina. The retina is a site of clinically relevant age-related alterations but has also proven to be a uniquely approachable system for discovering principles that govern neural aging because it is well mapped, contains diverse neuron types, and is experimentally accessible. In this article, we review the structural and molecular impacts of aging on neurons within the inner and outer retina circuits. We further discuss the contribution of non-neuronal cell types and systems to retinal aging outcomes. Understanding how and why the retina ages is critical to efforts aimed at preventing age-related neural decline and restoring neural function.


Asunto(s)
Sistema Nervioso Central , Neuronas , Retina , Factores de Riesgo
3.
Immunity ; 55(12): 2318-2335.e7, 2022 12 13.
Artículo en Inglés | MEDLINE | ID: mdl-36379210

RESUMEN

Microglia utilize their phagocytic activity to prune redundant synapses and refine neural circuits during precise developmental periods. However, the neuronal signals that control this phagocytic clockwork remain largely undefined. Here, we show that neuronal signal-regulatory protein alpha (SIRPα) is a permissive cue for microglial phagocytosis in the developing murine retina. Removal of neuronal, but not microglial, SIRPα reduced microglial phagocytosis, increased synpase numbers, and impaired circuit function. Conversely, prolonging neuronal SIRPα expression extended developmental microglial phagocytosis. These outcomes depended on the interaction of presynaptic SIRPα with postsynaptic CD47. Global CD47 deficiency modestly increased microglial phagocytosis, while CD47 overexpression reduced it. This effect was rescued by coexpression of neuronal SIRPα or codeletion of neuronal SIRPα and CD47. These data indicate that neuronal SIRPα regulates microglial phagocytosis by limiting microglial SIRPα access to neuronal CD47. This discovery may aid our understanding of synapse loss in neurological diseases.


Asunto(s)
Antígeno CD47 , Receptores Inmunológicos , Ratones , Animales , Antígeno CD47/metabolismo , Receptores Inmunológicos/metabolismo , Macrófagos/metabolismo , Fagocitosis/fisiología , Retina , Antígenos de Diferenciación/metabolismo
4.
Curr Biol ; 32(22): 4783-4796.e3, 2022 11 21.
Artículo en Inglés | MEDLINE | ID: mdl-36179691

RESUMEN

The formation of neural circuits occurs in a programmed fashion, but proper activity in the circuit is essential for refining the organization necessary for driving complex behavioral tasks. In the retina, sensory deprivation during the critical period of development is well known to perturb the organization of the visual circuit making the animals unable to use vision for behavior. However, the extent of plasticity, molecular factors involved, and malleability of individual channels in the circuit to manipulations outside of the critical period are not well understood. In this study, we selectively disconnected and reconnected rod photoreceptors in mature animals after completion of the retina circuit development. We found that introducing synaptic rod photoreceptor input post-developmentally allowed their integration into the circuit both anatomically and functionally. Remarkably, adult mice with newly integrated rod photoreceptors gained high-sensitivity vision, even when it was absent from birth. These observations reveal plasticity of the retina circuit organization after closure of the critical period and encourage the development of vision restoration strategies for congenital blinding disorders.


Asunto(s)
Retina , Células Fotorreceptoras Retinianas Bastones , Ratones , Animales , Células Fotorreceptoras Retinianas Bastones/fisiología , Retina/fisiología , Sinapsis/fisiología , Plasticidad Neuronal/fisiología
5.
Dis Model Mech ; 15(9)2022 09 01.
Artículo en Inglés | MEDLINE | ID: mdl-35972048

RESUMEN

Mutations in the potassium channel tetramerization domain-containing 7 (KCTD7) gene are associated with a severe neurodegenerative phenotype characterized by childhood onset of progressive and intractable myoclonic seizures accompanied by developmental regression. KCTD7-driven disease is part of a large family of progressive myoclonic epilepsy syndromes displaying a broad spectrum of clinical severity. Animal models of KCTD7-related disease are lacking, and little is known regarding how KCTD7 protein defects lead to epilepsy and cognitive dysfunction. We characterized Kctd7 expression patterns in the mouse brain during development and show that it is selectively enriched in specific regions as the brain matures. We further demonstrate that Kctd7-deficient mice develop seizures and locomotor defects with features similar to those observed in human KCTD7-associated diseases. We also show that Kctd7 is required for Purkinje cell survival in the cerebellum and that selective degeneration of these neurons is accompanied by defects in cerebellar microvascular organization and patterning. Taken together, these results define a new model for KCTD7-associated epilepsy and identify Kctd7 as a modulator of neuron survival and excitability linked to microvascular alterations in vulnerable regions.


Asunto(s)
Epilepsias Mioclónicas Progresivas , Células de Purkinje , Animales , Niño , Humanos , Ratones , Epilepsias Mioclónicas Progresivas/genética , Fenotipo , Canales de Potasio/genética , Convulsiones/genética
6.
Cell Rep Methods ; 2(7): 100253, 2022 07 18.
Artículo en Inglés | MEDLINE | ID: mdl-35880013

RESUMEN

Fine-scale molecular architecture is critical for nervous system and other biological functions. Methods to visualize these nanoscale structures would benefit from enhanced accessibility, throughput, and tissue compatibility. Here, we report RAIN-STORM, a rapid and scalable nanoscopic imaging optimization approach that improves three-dimensional visualization for subcellular targets in tissue at depth. RAIN-STORM uses conventional tissue samples and readily available reagents and is suitable for commercial instrumentation. To illustrate the efficacy of RAIN-STORM, we utilized the retina. We show that RAIN-STORM imaging is versatile and provide 3D nanoscopic data for over 20 synapse, neuron, glia, and vasculature targets. Sample preparation is also rapid, with a 1-day turnaround from tissue to image, and parameters are suitable for multiple tissue sources. Finally, we show that this method can be applied to clinical samples to reveal nanoscale features of human cells and synapses. RAIN-STORM thus paves the way for high-throughput studies of nanoscopic targets in tissue.


Asunto(s)
Imagenología Tridimensional , Neuronas , Humanos , Microscopía Fluorescente , Neuroglía , Sinapsis
7.
J Comp Neurol ; 530(8): 1247-1262, 2022 06.
Artículo en Inglés | MEDLINE | ID: mdl-34743323

RESUMEN

Obtaining a parts list of the sensory components of the retina is vital to understand the effects of light in behavior, health, and disease. Rods, cones, and intrinsically photosensitive retinal ganglion cells (ipRGCs) are the best described photoreceptors in the mammalian retina, but recent functional roles have been proposed for retinal neuropsin (Opn5)-an atypical opsin. However, little is known about the pattern of Opn5 expression in the retina. Using cre (Opn5cre ) and cre-dependent reporters, we uncover patterns of Opn5 expression and find that Opn5 is restricted to retinal ganglion cells (RGCs). Opn5-RGCs are nonhomogenously distributed through the retina, with greater densities of cells located in the dorsotemporal quadrant. In addition to the local topology of these cells, using cre-dependent AAV viral tracing, we surveyed their central targets and found that they are biased towards image-forming and image-stabilizing regions. Finally, molecular and electrophysiological profiling reveal that Opn5-RGCs comprise previously defined RGC types that respond optimally to edges and object-motion (F-mini-ONs, HD2, HD1, LEDs, ooDSRGCs, etc.). Together, these data describe the second collection of RGCs that express atypical opsins in the mouse, and expand the roles of image-forming cells in retinal physiology and function.


Asunto(s)
Retina , Células Ganglionares de la Retina , Animales , Mamíferos , Proteínas de la Membrana/metabolismo , Ratones , Opsinas/genética , Opsinas/metabolismo , Retina/fisiología , Células Fotorreceptoras Retinianas Conos/metabolismo , Células Ganglionares de la Retina/fisiología , Opsinas de Bastones/metabolismo
8.
Dev Biol ; 476: 218-239, 2021 08.
Artículo en Inglés | MEDLINE | ID: mdl-33848537

RESUMEN

Synapses in the outer retina are the first information relay points in vision. Here, photoreceptors form synapses onto two types of interneurons, bipolar cells and horizontal cells. Because outer retina synapses are particularly large and highly ordered, they have been a useful system for the discovery of mechanisms underlying synapse specificity and maintenance. Understanding these processes is critical to efforts aimed at restoring visual function through repairing or replacing neurons and promoting their connectivity. We review outer retina neuron synapse architecture, neural migration modes, and the cellular and molecular pathways that play key roles in the development and maintenance of these connections. We further discuss how these mechanisms may impact connectivity in the retina.


Asunto(s)
Células Fotorreceptoras/citología , Sinapsis/metabolismo , Visión Ocular/fisiología , Animales , Humanos , Interneuronas/fisiología , Células Fotorreceptoras/fisiología , Retina/fisiología , Células Fotorreceptoras Retinianas Conos/fisiología , Células Horizontales de la Retina/fisiología , Sinapsis/fisiología
9.
Cell Rep ; 34(5): 108698, 2021 02 02.
Artículo en Inglés | MEDLINE | ID: mdl-33535040

RESUMEN

Cone photoreceptors detect light and are responsible for color vision. These cells display a distinct polarized morphology where nuclei are precisely aligned in the apical retina. However, little is known about the mechanisms involved in cone nuclear positioning or the impact of this organization on retina function. We show that the serine/threonine kinase LKB1 and one of its substrates, AMPK, regulate cone nuclear positioning. In the absence of either molecule, cone nuclei are misplaced along the axon, resulting in altered nuclear lamination. LKB1 is required specifically in cones to mediate this process, and disruptions in nuclear alignment result in reduced cone function. Together, these results identify molecular determinants of cone nuclear position and indicate that cone nuclear position alignment enables proper visual function.


Asunto(s)
Proteínas Quinasas Activadas por AMP/metabolismo , Visión Ocular/fisiología , Animales , Ratones
10.
Front Neural Circuits ; 14: 583391, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-33177995

RESUMEN

During development, neurons generate excess processes which are then eliminated in concert with circuit maturation. C1q is the initiating protein in the complement cascade and has been implicated in this process, but whether C1q-mediated elimination is targeted to particular neural compartments is unclear. Using the murine retina, we identify C1q as a specific regulator of horizontal cell neurite confinement. Subsets of horizontal cell dendritic and axonal neurites extend into the outer retina suggesting that complement achieves both cellular and subcellular selectivity. These alterations emerge as outer retina synapses become mature. C1q expression is restricted to retina microglia, and the loss of C1q results in decreased microglia activation. This pathway appears independent of the C3a receptor (C3aR) and complement receptor 3 (CR3), as horizontal cells are normal when either protein is absent. Together, these data identify a new role for C1q in cell and neurite-specific confinement and implicate microglia-mediated phagocytosis in this process.


Asunto(s)
Complemento C1q/fisiología , Microglía/metabolismo , Neuritas/fisiología , Plasticidad Neuronal/fisiología , Animales , Complemento C3a , Ratones , Ratones Noqueados , Microglía/fisiología , Fagocitosis , Receptores de Complemento , Células Horizontales de la Retina
11.
Elife ; 92020 05 07.
Artículo en Inglés | MEDLINE | ID: mdl-32378514

RESUMEN

Structural changes in pre and postsynaptic neurons that accompany synapse formation often temporally and spatially overlap. Thus, it has been difficult to resolve which processes drive patterned connectivity. To overcome this, we use the laminated outer murine retina. We identify the serine/threonine kinase LKB1 as a key driver of synapse layer emergence. The absence of LKB1 in the retina caused a marked mislocalization and delay in synapse layer formation. In parallel, LKB1 modulated postsynaptic horizontal cell refinement and presynaptic photoreceptor axon growth. Mislocalized horizontal cell processes contacted aberrant cone axons in LKB1 mutants. These defects coincided with altered synapse protein organization, and horizontal cell neurites were misdirected to ectopic synapse protein regions. Together, these data suggest that LKB1 instructs the timing and location of connectivity in the outer retina via coordinate regulation of pre and postsynaptic neuron structure and the localization of synapse-associated proteins.


Asunto(s)
Neuritas/enzimología , Neurogénesis , Células Fotorreceptoras/enzimología , Proteínas Serina-Treonina Quinasas/metabolismo , Sinapsis/enzimología , Proteínas Quinasas Activadas por AMP , Animales , Femenino , Masculino , Ratones Noqueados , Mutación , Proteínas Serina-Treonina Quinasas/genética , Transporte de Proteínas , Proteína 1 de Transporte Vesicular de Glutamato/metabolismo
12.
J Comp Neurol ; 528(5): 729-755, 2020 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-31609468

RESUMEN

In several areas of the central nervous system, neurons are regionally organized into groups or layers that carry out specific activities. In this form of patterning, neurons of distinct types localize their cell bodies to just one or a few of the layers within a structure. However, little is known about whether diverse neuron types within a lamina share molecular features that coordinate their organization. To begin to identify such candidates, we used the laminated murine retina to screen 92 lacZ reporter lines available through the Knockout Mouse Project. Thirty-two of these displayed reporter expression in restricted subsets of inner retina neurons. We then identified the spatiotemporal expression patterns of these genes at key developmental stages. This uncovered several that were heavily enriched in development but reduced in adulthood, including the transcriptional regulator Hmga1. An additional set of genes displayed maturation associated laminar enrichment. Among these, we identified Bbox1 as a novel gene that specifically labels all neurons in the ganglion cell layer but is largely excluded from otherwise molecularly similar neurons in the inner retina. Finally, we established Dbn1 as a new marker enriched in amacrines and Fmnl3 as a marker for subsets of αRGCs. Together, these data provide a spatiotemporal map for laminae-specific molecules and suggest that diverse neuron types within a lamina share coordinating molecular features that may inform their fate or function.


Asunto(s)
Neuronas Retinianas/citología , Animales , Ratones
13.
Neuron ; 103(6): 959-963, 2019 09 25.
Artículo en Inglés | MEDLINE | ID: mdl-31557459

RESUMEN

Starting your own academic lab is a wonderful opportunity to impact science through research and trainee mentoring. In this article, we share some thoughts and resources for this undertaking in the hope that they may enhance the experience of others.


Asunto(s)
Selección de Profesión , Laboratorios/organización & administración , Cultura Organizacional , Selección de Personal , Investigadores , Planificación Estratégica , Humanos
14.
Neurochem Int ; 129: 104486, 2019 10.
Artículo en Inglés | MEDLINE | ID: mdl-31175897

RESUMEN

Neuron function relies on and instructs the development and precise organization of neurovascular units that in turn support circuit activity. However, our understanding of the molecular cues that regulate this relationship remains sparse. Using a high-throughput screening pipeline, we recently identified several new regulators of vascular patterning. Among these was the potassium channel tetramerization domain-containing protein 7 (KCTD7). Mutations in KCTD7 are associated with progressive myoclonic epilepsy, but how KCTD7 regulates neural development and function remains poorly understood. To begin to identify such mechanisms, we focus on mouse retina, a tractable part of the central nervous system that contains precisely ordered neuron subtypes supported by a trilaminar vascular network. We find that deletion of Kctd7 induces defective patterning of the adult retina vascular network, resulting in increased branching, vessel length, and lacunarity. These alterations reflect early and specific defects in vessel development, as emergence of the superficial and deep vascular layers were delayed. These defects are likely due to a role for Kctd7 in inner retina neurons. Kctd7 is absent from vessels but present in neurons in the inner retina, and its deletion resulted in a corresponding increase in the number of bipolar cells in development and increased vessel branching in adults. These alterations were accompanied by retinal function deficits. Together, these data suggest that neuronal Kctd7 drives growth and patterning of the vasculature and that neurovascular interactions may participate in the pathogenesis of KCTD7-related human diseases.


Asunto(s)
Canales de Potasio/fisiología , Vasos Retinianos/fisiología , Animales , Electrorretinografía , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Epilepsias Mioclónicas Progresivas/genética , Canales de Potasio/deficiencia , Canales de Potasio/genética , ARN Mensajero/biosíntesis , Retina/ultraestructura , Células Bipolares de la Retina/patología , Vasos Retinianos/crecimiento & desarrollo , Vasos Retinianos/patología
15.
Neural Dev ; 14(1): 12, 2019 12 30.
Artículo en Inglés | MEDLINE | ID: mdl-31888774

RESUMEN

Microglia are increasingly shown to be key players in neuron development and synapse connectivity. However, the underlying mechanisms by which microglia regulate neuron function remain poorly understood in part because such analysis is challenging in the brain where neurons and synapses are intermingled and connectivity is only beginning to be mapped. Here, we discuss the features and function of microglia in the ordered mammalian retina where the laminar organization of neurons and synapses facilitates such molecular studies. We discuss microglia origins and consider the evidence for molecularly distinct microglia subpopulations and their potential for differential roles with a particular focus on the early stages of retina development. We then review the models and methods used for the study of these cells and discuss emerging data that link retina microglia to the genesis and survival of particular retina cell subtypes. We also highlight potential roles for microglia in shaping the development and organization of the vasculature and discuss cellular and molecular mechanisms involved in this process. Such insights may help resolve the mechanisms by which retinal microglia impact visual function and help guide studies of related features in brain development and disease.


Asunto(s)
Microglía/fisiología , Retina/crecimiento & desarrollo , Animales
16.
Cell Rep ; 24(9): 2506-2519, 2018 08 28.
Artículo en Inglés | MEDLINE | ID: mdl-30157441

RESUMEN

Retinal function relies on precisely organized neurons and synapses and a properly patterned vasculature to support them. Alterations in these features can result in vision loss. However, our understanding of retinal organization pathways remains incomplete because of a lack of methods to rapidly identify neuron and vasculature regulators in mammals. Here we developed a pipeline for the identification of neural and synaptic integrity genes by high-throughput retinal screening (INSiGHT) that analyzes candidate expression, vascular patterning, cellular organization, and synaptic arrangement. Using this system, we examined 102 mutant mouse lines and identified 16 unique retinal regulatory genes. Fifteen of these candidates are identified as novel retina regulators, and many (9 of 16) are associated with human neural diseases. These results expand the genetic landscape involved in retinal circuit organization and provide a road map for continued discovery of mammalian retinal regulators and disease-causing alleles.


Asunto(s)
Neuronas/fisiología , Retina/fisiología , Humanos , Sinapsis
17.
Neuron ; 98(1): 109-126.e8, 2018 04 04.
Artículo en Inglés | MEDLINE | ID: mdl-29576390

RESUMEN

Screens for genes that orchestrate neural circuit formation in mammals have been hindered by practical constraints of germline mutagenesis. To overcome these limitations, we combined RNA-seq with somatic CRISPR mutagenesis to study synapse development in the mouse retina. Here synapses occur between cellular layers, forming two multilayered neuropils. The outer neuropil, the outer plexiform layer (OPL), contains synapses made by rod and cone photoreceptor axons on rod and cone bipolar dendrites, respectively. We used RNA-seq to identify selectively expressed genes encoding cell surface and secreted proteins and CRISPR-Cas9 electroporation with cell-specific promoters to assess their roles in OPL development. Among the genes identified in this way are Wnt5a and Wnt5b. They are produced by rod bipolars and activate a non-canonical signaling pathway in rods to regulate early OPL patterning. The approach we use here can be applied to other parts of the brain.


Asunto(s)
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas/fisiología , Mutagénesis/fisiología , Neurópilo/metabolismo , Retina/metabolismo , Análisis de Secuencia de ARN/métodos , Vía de Señalización Wnt/fisiología , Animales , Animales Recién Nacidos , Femenino , Masculino , Ratones , Ratones Transgénicos , Neurópilo/química , Conejos , Retina/química , Retina/crecimiento & desarrollo
18.
Nat Neurosci ; 17(9): 1190-7, 2014 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-25086610

RESUMEN

Age-related decreases in neural function result in part from alterations in synapses. To identify molecular defects that lead to such changes, we focused on the outer retina, in which synapses are markedly altered in old rodents and humans. We found that the serine/threonine kinase LKB1 and one of its substrates, AMPK, regulate this process. In old mice, synaptic remodeling was accompanied by specific decreases in the levels of total LKB1 and active (phosphorylated) AMPK. In the absence of either kinase, young adult mice developed retinal defects similar to those that occurred in old wild-type animals. LKB1 and AMPK function in rod photoreceptors where their loss leads to aberrant axonal retraction, the extension of postsynaptic dendrites and the formation of ectopic synapses. Conversely, increasing AMPK activity genetically or pharmacologically attenuates and may reverse age-related synaptic alterations. Together, these results identify molecular determinants of age-related synaptic remodeling and suggest strategies for attenuating these changes.


Asunto(s)
Proteínas Quinasas Activadas por AMP/genética , Envejecimiento/fisiología , Proteínas Serina-Treonina Quinasas/genética , Segmento Externo de la Célula en Bastón/patología , Segmento Externo de la Célula en Bastón/fisiología , Proteínas Quinasas Activadas por AMP/metabolismo , Envejecimiento/patología , Células Amacrinas/patología , Células Amacrinas/fisiología , Animales , Electrorretinografía , Femenino , Masculino , Ratones Endogámicos C57BL , Ratones Noqueados , Fosforilación/fisiología , Proteínas Serina-Treonina Quinasas/metabolismo , Células Bipolares de la Retina/patología , Células Bipolares de la Retina/fisiología , Células Ganglionares de la Retina/patología , Células Ganglionares de la Retina/fisiología , Especificidad por Sustrato , Sinapsis/patología , Sinapsis/fisiología
19.
J Virol ; 87(5): 2814-22, 2013 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-23269784

RESUMEN

The severity of West Nile virus (WNV) infection in immunocompetent animals is highly strain dependent, ranging from avirulent to highly neuropathogenic. Here, we investigate the nature of this strain-specific restriction by analyzing the replication of avirulent (WNV-MAD78) and highly virulent (WNV-NY) strains in neurons, astrocytes, and microvascular endothelial cells, which comprise the neurovascular unit within the central nervous system (CNS). We demonstrate that WNV-MAD78 replicated in and traversed brain microvascular endothelial cells as efficiently as WNV-NY. Likewise, similar levels of replication were detected in neurons. Thus, WNV-MAD78's nonneuropathogenic phenotype is not due to an intrinsic inability to replicate in key target cells within the CNS. In contrast, replication of WNV-MAD78 was delayed and reduced compared to that of WNV-NY in astrocytes. The reduced susceptibility of astrocytes to WNV-MAD78 was due to a delay in viral genome replication and an interferon-independent reduction in cell-to-cell spread. Together, our data suggest that astrocytes regulate WNV spread within the CNS and therefore are an attractive target for ameliorating WNV-induced neuropathology.


Asunto(s)
Astrocitos/virología , Células Endoteliales/virología , Replicación Viral , Virus del Nilo Occidental/fisiología , Animales , Línea Celular , Sistema Nervioso Central/virología , Chlorocebus aethiops , Humanos , Microvasos/citología , Microvasos/virología , Neuronas/virología , Células Vero , Fiebre del Nilo Occidental/transmisión , Fiebre del Nilo Occidental/virología
20.
PLoS One ; 7(10): e46663, 2012.
Artículo en Inglés | MEDLINE | ID: mdl-23056392

RESUMEN

As synapses form and mature the synaptic partners produce organizing molecules that regulate each other's differentiation and ensure precise apposition of pre- and post-synaptic specializations. At the skeletal neuromuscular junction (NMJ), these molecules include agrin, a nerve-derived organizer of postsynaptic differentiation, and synaptic laminins, muscle-derived organizers of presynaptic differentiation. Both become concentrated in the synaptic cleft as the NMJ develops and are retained in adulthood. Here, we used mutant mice to ask whether these organizers are also required for synaptic maintenance. Deletion of agrin from a subset of adult motor neurons resulted in the loss of acetylcholine receptors and other components of the postsynaptic apparatus and synaptic cleft. Nerve terminals also atrophied and eventually withdrew from muscle fibers. On the other hand, mice lacking the presynaptic organizer laminin-α4 retained most of the synaptic cleft components but exhibited synaptic alterations reminiscent of those observed in aged animals. Although we detected no marked decrease in laminin or agrin levels at aged NMJs, we observed alterations in the distribution and organization of these synaptic cleft components suggesting that such changes could contribute to age-related synaptic disassembly. Together, these results demonstrate that pre- and post-synaptic organizers actively function to maintain the structure and function of adult NMJs.


Asunto(s)
Agrina/metabolismo , Laminina/metabolismo , Unión Neuromuscular/metabolismo , Agrina/genética , Animales , Inmunohistoquímica , Laminina/genética , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Neuronas Motoras/metabolismo , Neuronas Motoras/fisiología , Fibras Musculares Esqueléticas/metabolismo , Fibras Musculares Esqueléticas/fisiología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Sinapsis/metabolismo , Sinapsis/fisiología
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