Nutrition by Design: Boosting Selenium Content and Fresh Matter Yields of Salad Greens With Preharvest Light Intensity and Selenium Applications.

Selenium (Se) is an essential mineral in multiple human metabolic pathways with immune modulatory effects on viral diseases including the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and HIV. Plant-based foods contain Se metabolites with unique functionalities for the human metabolism. In order to assess the value of common salad greens as Se source, we conducted a survey of lettuce commercially grown in 15 locations across the USA and Canada and found a tendency for Se to accumulate higher (up to 10 times) in lettuce grown along the Colorado river basin region, where the highest amount of annual solar radiation of the country is recorded. In the same area, we evaluated the effect of sunlight reduction on the Se content of two species of arugula [Eruca sativa (E. sativa) cv. "Astro" and Diplotaxis tenuifolia (D. tenuifolia) cv. "Sylvetta"]. A 90% light reduction during the 7 days before harvest resulted in over one-third Se decline in D. tenuifolia. The effect of light intensity on yield and Se uptake of arugula microgreens was also examined under indoor controlled conditions. This included high intensity (HI) (160 µ mol-2 s-1 for 12 h/12 h light/dark); low intensity (LI) (70 µ mol m-2 s-1 for 12 h/12 h light/dark); and HI-UVA (12 h light of 160 µ mol m-2 s-1, 2 h UVA of 40 µ mol m-2 s-1, and 10 h dark) treatments in a factorial design with 0, 1, 5, and 10 ppm Se in the growing medium. HI and HI-UVA produced D. tenuifolia plants with 25-100% higher Se content than LI, particularly with the two higher Se doses. The addition of Se produced a marked increase in fresh matter (>35% in E. sativa and >45% in D. tenuifolia). This study (i) identifies evidence to suggest the revision of food composition databases to account for large Se variability, (ii) demonstrates the potential of introducing preharvest Se to optimize microgreen yields, and (iii) provides the controlled environment industry with key information to deliver salad greens with targeted Se contents.

Assessing the Food Safety Risk Posed by Birds Entering Leafy Greens Fields in the US Southwest.

In the US Southwest, it is common to observe birds in leafy green fields, though the risk they contribute to foodborne outbreaks remains unclear. In this study, we investigated and recorded the relationship between birds near leafy green fields and the risk for contaminated irrigation water or leafy green plants. We monitored the presence of birds for over two years and performed cloacal swab analysis for non-pathogenic Escherichia coli, E. coli O157:H7 and Salmonellaenterica, while also monitoring the incidence of other microbial indicators. We also assessed the risks from bird feces by performing observations in a commercial field reported with Salmonella positive samples and by analyzing the survival of foodborne pathogens in bird feces. Our results showed that most of the birds near the crop fields were resident small birds. We did not observe a correlation between the number of birds in sites and the incidence of indicator bacteria (e.g., coliforms, E. coli) in irrigation canal water, with the exception of one out of four sites where water flow was low or stagnant. Using walk-in-traps, 305 birds were captured and placed in short-term captivity to determine the presence of various bacteria. None of the birds tested positive for E. coli O157:H7 or Salmonella. However, nearly 40% of the birds captured were confirmed positive for non-pathogenic E. coli. We found no correlation between age (young, adult, unknown), gender (male, female, unknown) and the incidence of E. coli positive birds, but we observed significantly higher probability of incidence during October-December. The role of relative humidity and temperature on bacterial survival appeared to play a key role in the survival of Salmonella on the leaves of spinach plants in a commercial field. This was also confirmed in laboratory conditions where Salmonella inoculated in bird feces and exposed to 15 °C and 80% RH(Relative humidity) survived beyond 133 days, while at 26 °C and 40% RH, the organism was undetectable after 63 days. Our results suggest that local birds associated with leafy green fields likely pose a minimal impact of risk for food contamination, but also points out the need for increased analysis specifically for E. coli O157:H7. Furthermore, our study suggests the need for expanding research that addresses risks associated with large migratory birds, especially in areas where stagnated water sources would be used for overhead sprinkle irrigation.

Activation of BK Channel Contributes to PL-Induced Mesenchymal Stem Cell Migration.

Due to their capacity to proliferate, migrate, and differentiate, mesenchymal stem cells (MSCs) are considered to be good candidates for regenerative medicine applications. The mechanisms underlying proliferation and differentiation of MSCs have been studied. However, much less is known about the mechanisms regulating the migration of MSCs. Platelet lysate (PL), a supplement used to promote cell expansion, has been shown to promote MSCs migration; however, the underlying mechanism are unknown. Here, by using adipose-derived rat MSCs (rMSCs) and the scratch assay in the absence and presence of various BK channels modulators, we evaluated the role of BK channels in mediating the PL-stimulated migration of rMSCs. We found that 5% PL increased rMSCs migration, and this effect was blocked by the addition of the BK channel selective antagonist Iberiotoxin (IBTX). In the absence of PL, the BK channel agonist NS1619, stimulated rMSCs migration to similar level as 5% PL. Addition of both NS1619 and 5% PL resulted in an increase in rMSCs migration, that was higher than when either one was added individually. From whole-cell recordings, it was found that the addition of 5% PL increased the magnitude of BK current density. By using Western blot and flow cytometry, it was found that PL did not affect the expression of BK channels. Together, our results indicate that as shown in other cell types, activation of BK channels by themselves also promote rMSC migration, and show that activation of BK channels contribute to the observed PL-induced increase in migration of rMSC.

Enhanced proton translocating pyrophosphatase activity improves nitrogen use efficiency in Romaine lettuce.

Plant nitrate (NO3(-)) acquisition depends on the combined activities of root high- and low-affinity NO3(-) transporters and the proton gradient generated by the plasma membrane H(+)-ATPase. These processes are coordinated with photosynthesis and the carbon status of the plant. Here, we present the characterization of romaine lettuce (Lactuca sativa 'Conquistador') plants engineered to overexpress an intragenic gain-of-function allele of the type I proton translocating pyrophosphatase (H(+)-PPase) of Arabidopsis (Arabidopsis thaliana). The proton-pumping and inorganic pyrophosphate hydrolytic activities of these plants are augmented compared with control plants. Immunohistochemical data show a conspicuous increase in H(+)-PPase protein abundance at the vasculature of the transgenic plants. Transgenic plants displayed an enhanced rhizosphere acidification capacity consistent with the augmented plasma membrane H(+)-ATPase proton transport values, and ATP hydrolytic capacities evaluated in vitro. These transgenic lines outperform control plants when challenged with NO3(-) limitations in laboratory, greenhouse, and field scenarios. Furthermore, we report the characterization of a lettuce LsNRT2.1 gene that is constitutive up-regulated in the transgenic plants. Of note, the expression of the LsNRT2.1 gene in control plants is regulated by NO3(-) and sugars. Enhanced accumulation of (15)N-labeled fertilizer by transgenic lettuce compared with control plants was observed in greenhouse experiments. A negative correlation between the level of root soluble sugars and biomass is consistent with the strong root growth that characterizes these transgenic plants.

Point sources of emerging contaminants along the Colorado River Basin: source water for the arid Southwestern United States.

Emerging contaminants (ECs) (e.g., pharmaceuticals, illicit drugs, personal care products) have been detected in waters across the United States. The objective of this study was to evaluate point sources of ECs along the Colorado River, from the headwaters in Colorado to the Gulf of California. At selected locations in the Colorado River Basin (sites in Colorado, Utah, Nevada, Arizona, and California), waste stream tributaries and receiving surface waters were sampled using either grab sampling or polar organic chemical integrative samplers (POCIS). The grab samples were extracted using solid-phase cartridge extraction (SPE), and the POCIS sorbents were transferred into empty SPEs and eluted with methanol. All extracts were prepared for, and analyzed by, liquid chromatography-electrospray-ion trap mass spectrometry (LC-ESI-ITMS). Log D(OW) values were calculated for all ECs in the study and compared to the empirical data collected. POCIS extracts were screened for the presence of estrogenic chemicals using the yeast estrogen screen (YES) assay. Extracts from the 2008 POCIS deployment in the Las Vegas Wash showed the second highest estrogenicity response. In the grab samples, azithromycin (an antibiotic) was detected in all but one urban waste stream, with concentrations ranging from 30ng/L to 2800ng/L. Concentration levels of azithromycin, methamphetamine and pseudoephedrine showed temporal variation from the Tucson WWTP. Those ECs that were detected in the main surface water channels (those that are diverted for urban use and irrigation along the Colorado River) were in the region of the limit-of-detection (e.g., 10ng/L), but most were below detection limits.

[A protocol in the form of small posters to assess the risks and the fragilities of elderly people in care homes]. / Un protocole sous forme d'affiche pour évaluer les risques et les fragilités des personnes âgées en Ehpad.

Nursing teams are obliged to have care protocols. Those which are presented in the form of small-size posters have the advantage of being pragmatic. A review of an experiment with a protocol which covers all the assessments which residents in care homes for dependent elderly people need.

Method development and application to determine potential plant uptake of antibiotics and other drugs in irrigated crop production systems.

Studies have shown the detection of emerging contaminants (ECs), of which pharmaceuticals are a subset, in surface waters across the United States. The objective of this study was to develop methods, and apply them, to evaluate the potential for food chain transfer when EC-containing waters are used for crop irrigation. Greenhouse experiments were performed in which select food crops were irrigated with water spiked with three antibiotics. Field experiments, at two different sites, were conducted. Select crops were irrigated with wastewater effluent known to contain ECs, EC-free well water, and Colorado River water containing trace-level ECs. The results of the greenhouse studies show the potential for uptake of one or more of the antibiotics evaluated, albeit at very low levels. In those food crops watered with wastewater effluent, only an industrial flavoring agent, N,N'-dimethylphenethylamine (DMPEA), was consistently found. None of the evaluated contaminants were found in crops irrigated with Colorado River water.

Detecting novel genes with sparse arrays.

Species-specific genes play an important role in defining the phenotype of an organism. However, current gene prediction methods can only efficiently find genes that share features such as sequence similarity or general sequence characteristics with previously known genes. Novel sequencing methods and tiling arrays can be used to find genes without prior information and they have demonstrated that novel genes can still be found from extensively studied model organisms. Unfortunately, these methods are expensive and thus are not easily applicable, e.g., to finding genes that are expressed only in very specific conditions. We demonstrate a method for finding novel genes with sparse arrays, applying it on the 33.9 Mb genome of the filamentous fungus Trichoderma reesei. Our computational method does not require normalisations between arrays and it takes into account the multiple-testing problem typical for analysis of microarray data. In contrast to tiling arrays, that use overlapping probes, only one 25 mer microarray oligonucleotide probe was used for every 100b. Thus, only relatively little space on a microarray slide was required to cover the intergenic regions of a genome. The analysis was done as a by-product of a conventional microarray experiment with no additional costs. We found at least 23 good candidates for novel transcripts that could code for proteins and all of which were expressed at high levels. Candidate genes were found to neighbour ire1 and cre1 and many other regulatory genes. Our simple, low-cost method can easily be applied to finding novel species-specific genes without prior knowledge of their sequence properties.

Perchlorate exposure from food crops produced in the lower Colorado River region.

The Colorado River shows low levels of perchlorate derived from aerospace- and defense-related fuel industries once located near the Las Vegas Wash. At sufficiently high dosages perchlorate can disrupt thyroid function by inhibiting uptake of iodide. The Colorado River is the primary source of irrigation water for most food crops grown in Southern California and Southwestern Arizona. The objective of this study was to evaluate potential perchlorate exposure from food crops produced in the lower Colorado River region (LCRR). The major food commodities produced in the region were sampled and perchlorate levels were determined by ion chromatography followed by detection using either conductivity or tandem mass spectrometry, depending on analyte levels. The Monte Carlo module of the Dietary Exposure Evaluation Model (DEEM) was used to derive an estimate of the 2-day average perchlorate intakes. Data were derived assuming that individuals residing in the LCRR get their fruits and vegetables from within the LCRR as well as from other areas in the United States, or assuming individuals living in the LCRR get their fruits and vegetables from the LCRR only. Perchlorate exposure estimates derived in this study are comparable to exploratory estimates by the US Food and Drug Administration. For infants and children, over 50% of the estimated perchlorate exposure was from milk. The relative impact of vegetables and fruit toward perchlorate exposure increased by age through adulthood. Cumulative perchlorate exposure estimates based on this hypothetical analysis could approach or exceed the NAS reference dose (RfD) for some population groups as drinking water levels exceeded 6 microg/l. However, few individuals are exposed to perchlorate in drinking water at levels above 4 microg/l in the United States and very few would be exposed to perchlorate levels exceeding the RfD, whether consuming food crops from within or outside the LCRR.