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1.
Eur J Pharmacol ; 797: 75-82, 2017 Feb 15.
Artículo en Inglés | MEDLINE | ID: mdl-28099869

RESUMEN

Amitriptyline, a tricyclic antidepressant, has been proposed as an antitumoral drug in oxidative therapy. Its pro-apoptotic effects, mediated by high reactive oxygen species generation, have been already described. In this study we analysed the effect of amitriptyline on the biosynthesis of coenzyme Q10 (CoQ), an essential component for electron transport and a potent membrane antioxidant involved in redox signaling. We treated H460 cells, a non-small-cell lung cancer cell line, with amitriptyline and we analysed CoQ levels by HPLC and CoQ biosynthesis rate, as well as the enzymes involved in CoQ biosynthesis by real-time PCR and Western blot. Amitriptyline treatment induced a dose-dependent decrease in CoQ levels in tumor cells. CoQ decreased levels were associated with down-regulation of the expression of COQ4 gene, as well as decreased Coq4 and Coq6 protein levels. Our findings suggest that the effect of amitriptyline on CoQ biosynthesis highlights the potential of this drug for antitumoral oxidative therapy.


Asunto(s)
Amitriptilina/farmacología , Neoplasias Pulmonares/patología , Ubiquinona/análogos & derivados , Línea Celular Tumoral , Relación Dosis-Respuesta a Droga , Humanos , Cinética , Especies Reactivas de Oxígeno/metabolismo , Ubiquinona/biosíntesis , Ubiquinona/metabolismo
2.
Mitochondrion ; 28: 54-9, 2016 May.
Artículo en Inglés | MEDLINE | ID: mdl-27017994

RESUMEN

Mitochondrial diseases are a group of clinically heterogeneous disorders that can be difficult to diagnose. We report a two and a half year old girl with clinical symptoms compatible with Leigh disease but with no definitive diagnosis. Using next generation sequencing we found that mutation 3697G>A was responsible for the patient's clinical symptoms. Corroboration was performed via segregation analysis in mother and sister and by evolutionary analysis that showed that the mutation is located in a highly conserved region across a wide range of species. Functional analyses corroborated the mutation effect and indicated that the pathophysiological alterations were partially restored by Coenzyme Q10. In addition, we proposed that the presence of the mutation at high frequencies causes the phenotype in the patient, while other family members with intermediate levels of heteroplasmy are symptoms-free.


Asunto(s)
Enfermedad de Leigh/genética , NADH Deshidrogenasa/genética , Mutación Puntual , Preescolar , ADN Mitocondrial/química , ADN Mitocondrial/genética , Femenino , Secuenciación de Nucleótidos de Alto Rendimiento , Humanos , Ubiquinona/análogos & derivados , Ubiquinona/uso terapéutico
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