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1.
Mutat Res ; 497(1-2): 213-22, 2001 Oct 18.
Artículo en Inglés | MEDLINE | ID: mdl-11525924

RESUMEN

Antioxidative and antimutagenic effect of yeast cell wall mannans, in particular, extracellular glucomannan (EC-GM) and glucomannan (GM-C.u.) both from Candida utilis, mannan from Saccharomyces cerevisiae (M-S.c.) and mannan from Candida albicans (M-C.a.) was evaluated. Luminol-dependent photochemical method using trolox as a standard showed that EC-GM, GM-C.u., M-S.c. and M-C.a. have relatively good antioxidative properties. EC-GM exhibited the highest antioxidative activity, followed by GM-C.u. and M-S.c. M-C.a. showed the least antioxidative activity. These mannans were experimentally confirmed to exhibit different, statistically significant antimutagenic activity in reducing damage of chloroplast DNA of the flagellate Euglena gracilis induced by ofloxacin and acridine orange (AO). We suggest that the antimutagenic effect of EC-GM, GM-C.u., M-S.c. and M-C.a. against ofloxacin is based on their ability to scavenge reactive oxygen radicals. With AO, the reduction of the chloroplast DNA lession could be a result of the absorptive capacity of the mannans. The important characteristics of mannans isolated from the yeast cell walls, such as good water solubility, relatively small molecular weight (15-30kDa), and antimutagenic effect exerted through different mode of action, appear to be a promising features for their prospective use as a natural protective (antimutagenic) agents.


Asunto(s)
Antimutagênicos/farmacología , Antioxidantes/farmacología , Mananos/farmacología , Naranja de Acridina/toxicidad , Animales , Candida/química , Candida albicans/química , Pared Celular/química , Daño del ADN , ADN de Cloroplastos/efectos de los fármacos , Euglena gracilis/efectos de los fármacos , Técnicas In Vitro , Luminol , Mananos/aislamiento & purificación , Pruebas de Mutagenicidad , Ofloxacino/toxicidad , Fotoquímica , Saccharomyces cerevisiae/química , Espectroscopía Infrarroja por Transformada de Fourier
2.
Biotechnol Appl Biochem ; 31(2): 153-9, 2000 04.
Artículo en Inglés | MEDLINE | ID: mdl-10744960

RESUMEN

The interaction of four lectins from crops of the legume family with Saccharomyces cerevisiae alpha-mannan, and also with two glycoenzymes containing mainly alpha-mannan moieties, has been studied. The interaction was characterized by a quantitative precipitation assay. The results of precipitation differ with respect to both quality (the point of maximum precipitation) and of the quantity (the amount of aggregated lectin and saccharide). The lectin concanavalin A [Con A, from jack bean (Canavalia ensiformis)] was observed to form more extensive precipitates with Saccharomyces cerevisiae mannan and glycoenzymes than did lectins from Lens culinaris (lentil) and Pisum sativum (garden pea), while in the case of Vicia faba (broad or fava bean) no interaction was found with either the examined mannans or with glycosylated enzymes. The complete precipitation of invertase and glucoamylase with Con A (enzymes and also Con A; up to 100%) was achieved at a Con A glycoenzyme molar ratio of 20.2 and 2.3 respectively, whereby about 85% of precipitated and also of initial activities of glycoenzymes were determined in the aggregates. More valuable results were achieved by the technique of enzyme immobilization called 'multiple bioaffinity layering' which is based on the stepwise biospecific adsorption of the glycosylated enzymes and Con A on a matrix precoupled with Con A. A 3-fold repetition of the layering procedure afforded up to a 10-fold increase in catalytic activity of the immobilized invertase, in contrast with a 2.1-fold increase in catalytic activity of the immobilized glucoamylase.


Asunto(s)
Enzimas Inmovilizadas/aislamiento & purificación , Mananos/aislamiento & purificación , Marcadores de Afinidad , Biotecnología , Precipitación Química , Concanavalina A , Fabaceae , Glucano 1,4-alfa-Glucosidasa/aislamiento & purificación , Glicósido Hidrolasas/aislamiento & purificación , Glicosilación , Lectinas , Mananos/química , Lectinas de Plantas , Plantas Medicinales , beta-Fructofuranosidasa
3.
Drugs Exp Clin Res ; 26(5-6): 279-83, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-11345039

RESUMEN

Ukrain has previously been demonstrated to exert a malignotoxic effect in vivo. This antitumor drug has been effective in the treatment of some malignancies in experimental animals as a result of immunostimulation (macrophage stimulation). In the present study, serum chitotriosidase activity was measured as a biochemical marker of macrophage stimulation in several murine and rat models of macrophage stimulation. It was shown that zymosan, carboxymethylated glucan and Triton WR 1339 administration to CBA mice or Wistar rats was followed by a considerable increase in serum chitotriosidase activity. Murine LS lymphosarcoma development decreased serum chitotriosidase activity. Antitumor treatment by Ukrain or cyclophosphamide did not restore this index to the normal value.


Asunto(s)
Alcaloides/farmacología , Antineoplásicos Alquilantes/farmacología , Antineoplásicos Fitogénicos/farmacología , Ciclofosfamida/farmacología , Hexosaminidasas/metabolismo , Linfoma no Hodgkin/tratamiento farmacológico , Linfoma no Hodgkin/enzimología , Macrófagos/efectos de los fármacos , Macrófagos/enzimología , Neoplasias Experimentales/tratamiento farmacológico , Neoplasias Experimentales/enzimología , Animales , Alcaloides de Berberina , Biomarcadores de Tumor , Hexosaminidasas/sangre , Hígado/patología , Masculino , Ratones , Ratones Endogámicos CBA , Fenantridinas
4.
Drugs Exp Clin Res ; 26(5-6): 301-6, 2000.
Artículo en Inglés | MEDLINE | ID: mdl-11345042

RESUMEN

Cystatin C is the best known extracellular endogenous cysteine proteinase inhibitor and has been studied as a possible index of tumor growth and as a marker of the effectiveness of antitumor therapy. The aim of this study was to evaluate cystatin C concentrations in murine tumor tissues (compared with other organs not directly involved with tumor development, such as the liver and spleen) during treatment with several antitumor drugs (Ukrain and/or cyclophosphane). Cystatin C concentrations in murine tissues and biological fluids was determined by enzyme-linked immunosorbent (ELISA) assay. The cystatin C ELISA test is a sandwich immunoassay, which uses immobilized rabbit antihuman cystatin C Pab and mouse antihuman cystatin C Mab-HRP (monoclonal antibodies, conjugated with horseradish peroxidase). We observed decreased serum cystatin C concentrations compared with controls in all nontreated tumor models: HA-1 hepatoma (solid and ascitic forms), lung adenocarcinoma (solid and ascitic forms) and LS lymphosarcoma. In the ascitic fluid of mice with HA-1 hepatoma the cystatin C concentration was much lower than in the serum of the same mice (about 20-fold lower). In the HA-1 model of hepatoma cells cystatin C concentration decreased about 2-3-fold compared with the control (intact liver) and Ukrain significantly increased the cystatin C concentration. Cyclophosphane treatment of LS lymphosarcoma significantly increased the cystatin C concentration in serum. Cyclophosphane treatment (50 mg/kg, single injection) increased cystatin C by up to 8-fold more in tumor issue. Ukrain treatment of LS lymphosarcoma was also followed by increased levels of cystatin C in tumor tissue (4-fold); cyclophosphane plus Ukrain had a similar positive effect. In the group with LS lymphosarcoma Ukrain or cyclophosphane plus Ukrain treatment induced a significant increase in cystatin C concentration in liver. Liver cystatin C concentration decreased in the HA-1 hepatoma group and treatment with Ukrain or carboxymethylated beta-1, 3-glucan (CMG) increased this index in both groups. Spleen cystatin C concentrations decreased about 5-fold in LS lymphosarcoma compared with controls and combined treatment with cyclophosphane plus Ukrain restored the index to the normal value. We can conclude that both murine tumors studied were characterized by low cystatin C concentrations in tumor tissues and decreased cystatin C concentrations (to a lesser degree) were also observed in liver and spleen as a result of the "toxic" effect of tumor bearing. Effective treatment in all cases (especially with Ukrain or a combination of cyclophosphane plus Ukrain) induced a significant increase in cystatin C. Obviously, the decrease in cystatin C concentration predominantly in tumor tissue was connected with tumor development and restoration of cystatin C level may be used as a marker of efficacy of antitumor therapy.


Asunto(s)
Alcaloides/uso terapéutico , Antineoplásicos Fitogénicos/uso terapéutico , Inhibidores de Cisteína Proteinasa/metabolismo , Neoplasias Experimentales/metabolismo , Animales , Alcaloides de Berberina , Biomarcadores de Tumor , Cistatina C , Cistatinas/metabolismo , Neoplasias Hepáticas Experimentales/tratamiento farmacológico , Neoplasias Hepáticas Experimentales/metabolismo , Neoplasias Pulmonares/tratamiento farmacológico , Neoplasias Pulmonares/metabolismo , Linfoma no Hodgkin/tratamiento farmacológico , Linfoma no Hodgkin/metabolismo , Ratones , Neoplasias Experimentales/tratamiento farmacológico , Fenantridinas , Distribución Tisular
5.
Mutat Res ; 444(1): 117-22, 1999 Jul 21.
Artículo en Inglés | MEDLINE | ID: mdl-10477345

RESUMEN

Glucomannan (GM) isolated from Candida utilis with molecular weight 30 kDa was administered either intraperitoneally or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei was evaluated in polychromatic erythrocytes of mouse bone marrow. GM administration by either route decreased significantly (p<0.002) the clastogenic effect of CP. The protective effect was concentration-dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg b. wt. (body weight). The fact that GM was effective also at oral administration is indicative of the passage of GM molecules through the wall of the gastrointestinal tract. The important characteristics of GM isolated from C. utilis, such as good water solubility, relatively small molecular weight (30 kDa), and antimutagenic effect exerted also at oral administration, appear to be promising features for its prospective use as a natural protective agent.


Asunto(s)
Antimutagênicos/farmacología , Ciclofosfamida/toxicidad , Mananos/farmacología , Mutágenos/toxicidad , Animales , Antimutagênicos/administración & dosificación , Antimutagênicos/química , Células de la Médula Ósea/efectos de los fármacos , Candida , Femenino , Mananos/administración & dosificación , Mananos/química , Ratones , Ratones Endogámicos ICR , Pruebas de Micronúcleos , Peso Molecular
6.
Ultrason Sonochem ; 6(1-2): 111-4, 1999 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-11233930

RESUMEN

Sonication was effective for the depolymerization of carboxymethylated chitin-glucan complex (CM-CG) isolated from the cell wall of Aspergillus niger. After 10 min of sonication and subsequent gel filtration, two samples (CM-CG(I) and CM CG(II)) with significantly distinct molecular weights (660 kDa and 19 kDa, respectively) and different nitrogen contents (3.02 and 1.69%) were obtained. CM-CG(II) with lower Mw was also effective against cyclophosphamide mutagenicity by oral administration in mice.


Asunto(s)
Antimutagênicos/síntesis química , Antimutagênicos/farmacología , Aspergillus niger/química , Quitina/química , Glucanos/química , Animales , Pared Celular/química , Fenómenos Químicos , Química Física , Cromatografía Líquida de Alta Presión , Ciclofosfamida/antagonistas & inhibidores , Ciclofosfamida/toxicidad , Femenino , Ratones , Ratones Endogámicos ICR , Mutágenos/toxicidad , Ultrafiltración , Ultrasonido
7.
Ultrason Sonochem ; 5(4): 169-72, 1999 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-11269957

RESUMEN

Different factors affecting the efficiency of the ultrasonic depolymerization of the high-molecular-weight carboxymethylated chitin-glucan prepared from the fungal mycelium of Aspergillus niger have been investigated. The influence of the following parameters was examined: concentration of the chitin-glucan complex, duration of ultrasonic irradiation, reaction temperature and volume of the ultrasonicated solution. The optimized conditions for the efficient ultrasonic depolymerization include: polysaccharide concentration--0.2 mg ml-1; volume of the sonicated solution--25 ml; duration of the sonication--10 min; and constant cooling of the sonicated sample in an ice-water bath.


Asunto(s)
Aspergillus niger/química , Quitina/química , Glucanos/química , Cromatografía Líquida de Alta Presión , Peso Molecular , Ultrasonido
8.
Mutat Res ; 412(1): 83-9, 1998 Jan 13.
Artículo en Inglés | MEDLINE | ID: mdl-9508367

RESUMEN

Carboxymethyl-chitin-glucan (CMCG) isolated from Aspergillus niger was ultrasonicated to decrease its molecular weight. Ultrasonicated CMCG with molecular weight 0.19 x 10(-5) was administered either intraperitoneally or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei in polychromatic erythrocytes of mouse bone marrow was evaluated. Both ways of CMCG administration significantly decreased the clastogenic effect of CP. The protective effect of CMCG was concentration dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg b.wt. Ultrasonic depolymerization of high molecular CMCG resulted in its anticlastogenic effect against CP not only on intraperitoneal, but also on oral administration, achieved by decreasing its molecular weight. Ultrasonication proved to be an efficient way to obtain molecules of CMCG able to pass through the cell walls of the gastrointestinal tract.


Asunto(s)
Antimutagênicos/farmacología , Células de la Médula Ósea/efectos de los fármacos , Quitina/análogos & derivados , Ciclofosfamida/toxicidad , Eritrocitos/efectos de los fármacos , Glucanos/farmacología , Pruebas de Micronúcleos , Mutágenos/toxicidad , Ultrasonido , Administración Oral , Animales , Antimutagênicos/administración & dosificación , Antimutagênicos/aislamiento & purificación , Aspergillus niger , Células de la Médula Ósea/patología , Quitina/administración & dosificación , Quitina/aislamiento & purificación , Quitina/farmacología , Cromatografía en Gel , Femenino , Glucanos/administración & dosificación , Glucanos/aislamiento & purificación , Inyecciones Intraperitoneales , Ratones , Ratones Endogámicos ICR
9.
Mutat Res ; 371(1-2): 115-20, 1996 Nov 04.
Artículo en Inglés | MEDLINE | ID: mdl-8950357

RESUMEN

Carboxymethylglucan (CMG) with ultrasonically lowered molecular weight (0.89 x 10(5)) was administered either intraperitoneally, intravenously or orally prior to cyclophosphamide (CP) injection and its effect on the frequency of micronuclei in mouse bone marrow was evaluated. Both parenteral (intraperitoneal and intravenous) and oral administration of CMG decreased the clastogenic effect of CP. The protective effect induced by intravenous and intraperitoneal administration was concentration-dependent, with a higher decrease achieved by 200 mg/kg than by 100 mg/kg body weight. With the lower dose of CMG a 2-h interval was necessary between intravenous CMG administration and CP injection. Oral pretreatment of mice with CMG decreased statistically significantly the frequency of micronuclei in polychromatic erythrocytes of the bone marrow. The fact that ultrasonically depolymerized CMG was effective also on oral administration is indicative of the passage of smaller CMG molecules through the wall of the gastrointestinal tract.


Asunto(s)
Ciclofosfamida/toxicidad , Glucanos/farmacología , Mutágenos/toxicidad , beta-Glucanos , Animales , Femenino , Glucanos/química , Ratones , Ratones Endogámicos ICR , Peso Molecular
10.
Int J Biol Macromol ; 17(6): 323-6, 1995 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-8789333

RESUMEN

Particulate beta-D-glucan was isolated from baker's yeast using autolysis and delipidization of the cells, followed by alkaline and acid treatment. The residual water-insoluble glucan termed cerevan has a beta-(1-->3)-linked backbone with beta-(1-->6)-linked short side chains. In order to achieve water solubility of the glucan, various derivatives were prepared (carboxymethyl-,carboxyethyl-,hydroxyethyl-,sulfoethyl-), and the beta-glucan was oxidized to glucuronoglucan. Their solubility, degree of substitution (DS), and molecular weight distribution (Mw) were compared. The immunomodulatory activity of these preparations was investigated in mitogenic and co-mitogenic tests on rat thymocytes. Cerevan showed higher stimulation indices compared with the known immunomodulator zymosan. Of the water-soluble derivatives, sulfoethylglucan was found to be the most active. Of the carboxymethyl derivatives of various DS, the preparation with DS = 0.75 exhibited the highest activity. Water-soluble carboxymethyl preparations with DS > 1.0 and low-molecular-weight glucuronoglucan were inactive.


Asunto(s)
Glucanos/farmacología , Activación de Linfocitos/efectos de los fármacos , Mitógenos/farmacología , Saccharomyces cerevisiae/química , Linfocitos T/efectos de los fármacos , beta-Glucanos , Adyuvantes Inmunológicos/aislamiento & purificación , Adyuvantes Inmunológicos/farmacología , Alquilación , Animales , Pared Celular/química , Glucanos/química , Glucanos/aislamiento & purificación , Metilación , Oxidación-Reducción , Tamaño de la Partícula , Ratas , Ratas Wistar , Solubilidad , Zimosan/farmacología
11.
Int J Immunopharmacol ; 17(3): 167-74, 1995 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-7558510

RESUMEN

The hemopoiesis-enhancing ability of a soluble glucan derivative, i.e. carboxymethylglucan (CMG), was investigated in gamma-irradiated mice. Attention was focused on the usefulness of its single or repeated postirradiation administration. CMG was administered i.p. at (a) single dose of 6 mg 2 h postirradiation, (b) four 6 mg doses in the first 4 days postirradiation, (c) four 1.5 mg doses at the same time intervals. Indices of granulopoiesis and inflammatory side effects (liver weight increase and hepatic granulomas) were investigated in mice irradiated with a sublethal dose of 7 Gy. All three CMG-treated groups of mice were found to exhibit enhanced hemopoietic recovery in comparison with the controls. Although the mice repeatedly given the 6 mg CMG doses showed the most rapid recoveries of all the evaluated parameters of granulopoiesis, the most pronounced hepatic side effects were found in these mice, too. When survival of mice was recorded in lethally (9 Gy) irradiated animals, the best protective response were obtained following the repeated administration of the 1.5 mg CMG dose, the survival by day 30 in this group being significantly higher not only in comparison with the controls but also with the mice repeatedly given the 6 mg dose of CMG. The results suggest that the postirradiation CMG administration can be useful for enhancing radiation suppressed hemopoiesis. However, repeated larger CMG doses may produce side effects which compromise the overall survival of irradiated mice.


Asunto(s)
Glucanos/farmacología , Hematopoyesis/efectos de los fármacos , Traumatismos Experimentales por Radiación/terapia , beta-Glucanos , Animales , Médula Ósea/efectos de los fármacos , Endotoxinas/toxicidad , Glucanos/efectos adversos , Hígado/efectos de los fármacos , Masculino , Ratones , Traumatismos Experimentales por Radiación/patología , Células Madre/efectos de los fármacos
12.
Mutat Res ; 346(1): 43-8, 1995 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-7530328

RESUMEN

The effect of high molecular carboxymethyl-chitin-glucan (CMCG), administered either intraperitoneally, intravenously or orally prior to cyclophosphamide injection, on the frequency of micronucleated reticulocytes was evaluated in peripheral blood of female ICR mice. Both intraperitoneal and intravenous administration of CMCG decreased the clastogenic effect of cyclophosphamide. The protective effect of CMCG was concentration dependent, with a higher decrease achieved by 100 mg/kg than by 50 mg/kg body weight. On the other hand, not even five peroral pretreatments with CMCG in the dose of 200 mg/kg body weight during the week prior to simultaneous administration of CMCG and cyclophosphamide induced a decrease of micronucleated reticulocytes in peripheral blood. It is therefore conceivable that CMCG failed to pass through the gastrointestinal tract, probably due to its high molecular weight. The antimutagenic effect of CMCG against cyclophosphamide was manifested by its intraperitoneal and intravenous administration to female ICR mice.


Asunto(s)
Antimutagênicos/farmacología , Quitina/análogos & derivados , Ciclofosfamida/farmacología , Glucanos/farmacología , Mutagénesis , Mutágenos/farmacología , Animales , Quitina/administración & dosificación , Quitina/farmacología , Vías de Administración de Medicamentos , Femenino , Glucanos/administración & dosificación , Ratones , Ratones Endogámicos ICR , Pruebas de Mutagenicidad , Reticulocitos/patología
13.
Folia Biol (Praha) ; 41(5): 249-56, 1995.
Artículo en Inglés | MEDLINE | ID: mdl-8714774

RESUMEN

Carboxymethylglucan (CMG), a water-soluble glucan derivative, enhanced the number of granulocytes in the peripheral blood as well as other indices of haemopoietic recovery (total cellularity and the number of granulocyte-macrophage progenitor cells in femoral marrow, spleen weight) investigated after fractionated gamma-irradiation in mice (five doses of 2 Gy each, or three, four and five doses of 3 Gy each given at 24-h intervals). An increased liver weight and a more pronounced anaemia found in the CMG-treated mice suggested, however, that also inflammatory side effects were evoked by the repeated administration of CMG. On the other hand, the development of tolerance, i.e., a decreased effectiveness of the treatment with CMG upon its repeated administration, did not seem to play any major role under the experimental conditions studied, because the protective effects of CMG increased with the increasing number of CMG injections.


Asunto(s)
Glucanos/administración & dosificación , Hematopoyesis/efectos de los fármacos , Adyuvantes Inmunológicos/administración & dosificación , Animales , Hematopoyesis/efectos de la radiación , Masculino , Ratones , Irradiación Corporal Total
14.
Folia Biol (Praha) ; 41(2): 112-7, 1995.
Artículo en Inglés | MEDLINE | ID: mdl-7656994

RESUMEN

Our results describe a novel carboxymethylglucan (CMG) activity, namely its radiosensitizing effect on early cellular damage in mice gamma-irradiated with a dose of 1 Gy. An increase of thymidine levels in blood plasma, determined 4 h after irradiation, was used as an indicator of the early cell death. The radiosensitizing effect was observed when administering CMG at time intervals close to irradiation time (1 h before to 1 h after irradiation). Diclofenac (an inhibitor of prostaglandin production) had no modifying effects on elevation of plasma thymidine levels induced by radiation or radiation + CMG. Pentoxifylline (an inhibitor of synthesis of tumour necrosis factor and of phosphodiesterase) administration elevated plasma thymidine to similar levels as CMG alone, combined pentoxifylline + CMG treatment had not additive effects.


Asunto(s)
Muerte Celular , Células/efectos de los fármacos , Células/efectos de la radiación , Rayos gamma , Glucanos/farmacología , Fármacos Sensibilizantes a Radiaciones/farmacología , beta-Glucanos , Animales , Diclofenaco/farmacología , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Pentoxifilina/farmacología , Timidina/sangre , Factores de Tiempo , Irradiación Corporal Total
15.
Mutat Res ; 302(4): 207-11, 1993 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-7688859

RESUMEN

The effect of pretreatment with sulfoethylglucan (SEG) on the frequency of micronuclei and the liver alkaline phosphatase activity induced by potassium bichromate (Cr(VI)) in mice was evaluated. Simultaneous application of SEG and Cr(VI) decreased the frequency of micronuclei in bone marrow cells (P < 0.01) and the level of liver alkaline phosphatase activity in comparison to the Cr(VI) group. Pretreatment with SEG 24 h prior to the first Cr(VI) application resulted in a more pronounced decrease in the Cr(VI)-induced frequency of micronuclei. The mechanisms of the protective effects of sulfoethylglucan could be explained either by the formation of Cr ion complexes with sulfoethyl groups of glucan or by the scavenging ability of SEG to trap hydroxyl radicals.


Asunto(s)
Antimutagênicos/farmacología , Glucanos/farmacología , Mutágenos/toxicidad , Dicromato de Potasio/antagonistas & inhibidores , Fosfatasa Alcalina/efectos de los fármacos , Fosfatasa Alcalina/metabolismo , Animales , Células de la Médula Ósea , Femenino , Hígado/enzimología , Ratones , Ratones Endogámicos ICR , Pruebas de Micronúcleos , Dicromato de Potasio/toxicidad
16.
J Rheumatol ; 20(4): 673-7, 1993 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-8496863

RESUMEN

Methyl-alpha-D-mannopyranoside, mannooligosaccharides obtained by acetolysis of yeast mannan, and pure mannans isolated from the cell walls of pathogenic (Candida albicans) and nonpathogenic (Saccharomyces cerevisiae) yeasts were used for treatment of rat adjuvant arthritis. The arthritis was induced by the application of Freund's complete adjuvant into the tail region of rats. The mannose substances were injected into the arthritic rats intraperitonealy at different time intervals. Levels of serum albumin, changes in hindpaws swelling and radiographs were measured in infected rats as variables of the inflammation and destructive arthritic changes. While mannan from C. albicans inhibited both the inflammation and destructive arthritic changes, mannan from S. cerevisiae showed less effect. However, acetolysate of S. cerevisiae mannan as well as simple methyl-alpha-D-mannopyranoside inhibited both inflammation and destructive arthritic changes to a similar degree as mannan isolated from C. albicans. The effect, which is not dose dependent indicates its possible immunoregulatory mechanism. This is the first time a therapeutic effect of simple carbohydrates on rat adjuvant arthritis has been described.


Asunto(s)
Artritis Experimental/prevención & control , Mananos/farmacología , Metilmanósidos/farmacología , Oligosacáridos/farmacología , Levaduras/metabolismo , Animales , Artritis Experimental/complicaciones , Artritis Experimental/diagnóstico por imagen , Edema/etiología , Pie , Miembro Posterior , Masculino , Mananos/metabolismo , Radiografía , Ratas , Ratas Endogámicas Lew , Albúmina Sérica/análisis
17.
Folia Biol (Praha) ; 39(4): 178-87, 1993.
Artículo en Inglés | MEDLINE | ID: mdl-8187896

RESUMEN

A moderate marrow granulocytic hyperplasia developed after 4 injections of glucan (soluble derivative carboxymethylglucan) administered to mice at 3-4-day intervals. However, when evaluating the response of the marrow granulocyte-macrophage colony-forming cells (GM-CFC) to the fifth glucan injection given in the repetitive treatment schedule, the development of hyporesponsiveness of these cells was found, contrary to the stimulatory action of a single glucan injection. In addition, the prompt increase in serum granulocyte-macrophage colony-stimulating activity occurring after a single glucan injection, and proposed to upregulate myelopoiesis, was absent in mice treated with glucan repeatedly. A joint administration of glucan with diclofenac, an inhibitor of prostaglandin synthesis, was able to enhance the GM-CFC response to a single glucan injection, probably due to the removal of the downregulation action of prostaglandins on these progenitor cells. However, a repeated administration of diclofenac with glucan did not reduce substantially the development of GM-CFC hyporesponsiveness. Thus, the results suggest that the GM-CFC hyporesponsiveness or tolerance to repeated glucan injections was induced by weakening the mechanisms of positive control mediated by the serum colony-stimulating activity.


Asunto(s)
Adyuvantes Inmunológicos/farmacología , Glucanos/farmacología , Células Madre Hematopoyéticas/patología , Activación de Macrófagos , beta-Glucanos , Animales , Médula Ósea/patología , Ensayo de Unidades Formadoras de Colonias , Factores Estimulantes de Colonias/biosíntesis , Diclofenaco/farmacología , Granulocitos/efectos de los fármacos , Granulocitos/patología , Células Madre Hematopoyéticas/efectos de los fármacos , Hiperplasia , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA
18.
Folia Microbiol (Praha) ; 38(3): 219-24, 1993.
Artículo en Inglés | MEDLINE | ID: mdl-8365697

RESUMEN

Structure of the glucomannan isolated from the cell walls of Candida utilis has been investigated using acetolysis fragmentation, methylation analysis and NMR spectroscopy. The structure of the glucomannan resembles that of the cellular mannans of other Candida species, except that the longer tetra- and pentassacharide side-chains are terminated with a glucosyl residue. Presence of the nonreducing glucosyl groups at the ends of the side-chains caused the C. utilis not to cross-react in a double immunodiffusion test with other Candida species that possess mannan antigens and cross-react with Hansenula species with glucomannan antigens.


Asunto(s)
Candida/química , Mananos/aislamiento & purificación , Secuencia de Carbohidratos , Cromatografía en Gel , Inmunodifusión , Espectroscopía de Resonancia Magnética , Mananos/química , Datos de Secuencia Molecular
19.
Exp Hematol ; 20(7): 891-5, 1992 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-1628707

RESUMEN

The effects of diclofenac (inhibitor of prostaglandin production) and carboxymethylglucan (immunomodulator and an agent stimulating hematopoiesis), when given to mice 1 day before gamma-irradiation, were studied. Both of the agents were administered either alone or in combination. The investigations included the assessment of post-irradiation hematopoietic recovery in terms of bone marrow and spleen cellularity and endogenous spleen colony formation, as well as the determination of the survival of lethally irradiated mice. The results demonstrated at least additive radioprotective effects when mice were given diclofenac and carboxymethylglucan in combination. Experimental evidence provided by the increased 125iodo-deoxyuridine incorporation into the spleen and elevated hydroxyurea kill of endogenous spleen colony-forming units indicated that the beneficial action of the combined treatment could be a consequence of increased cell proliferation in the hematopoietic tissue. It is likely that the inhibition of prostaglandin production (diclofenac action) and the concomitant increased release of growth factors (glucan action) shift the regulatory balance towards the predominance of positive hematopoietic control.


Asunto(s)
Diclofenaco/farmacología , Glucanos/farmacología , Hematopoyesis/efectos de la radiación , Protectores contra Radiación/farmacología , Irradiación Corporal Total , Animales , Médula Ósea/efectos de la radiación , Células de la Médula Ósea , División Celular/efectos de los fármacos , Sinergismo Farmacológico , Quimioterapia Combinada , Rayos gamma , Activación de Macrófagos/efectos de los fármacos , Masculino , Ratones , Premedicación , Bazo/citología , Bazo/efectos de la radiación
20.
Bratisl Lek Listy ; 92(6): 283-90, 1991 Jun.
Artículo en Checo | MEDLINE | ID: mdl-1873734

RESUMEN

The particulate glucan (G1), soluble glucan preparations (G2 to G5, and G7) isolated from Saccharomyces cerevisiae, and glucomanan prepared from culture fluid after cultivation of Candida utilis (G6) were tested for their immunomodulatory activity in vivo and in vitro. In tests in vivo three soluble glucans (G3, G4, and G7) injected s.c. to mice in the dose of 10 mg/kg increased the cytotoxic activity of peritoneal macrophages. The influence of glucans on natural killer cells was without significance. The lymphoproliferative reaction of spleen cells to polyclonal mitogens was inhibited by all the preparations used with the exception of soluble glucan G2. The mitogenic effect of the preparations, co-stimulatory tests and direct cytotoxicity to cells of cell lines used in cytotoxicity assays were assessed in vitro. The transformation index of glucans in the study was increased according to the glucan and dose tested. Inhibition of the lymphoproliferative reaction measured by the co-stimulatory test for optimal concentration of Concanavalin A occurred in a wide range of doses for the preparations G1 to G6. The preparation G7 increased the incorporation of 3HTdR under the same conditions. The use of a suboptimal concentration of Concanavalin A revealed co-stimulatory activity of all the preparations tested. Assessment of the cytotoxic activity of peritoneal macrophages and of the activity of natural killer cells induced in vitro was complicated by the direct cytotoxicity of particulate glucan and soluble glucan G5 (carboxymethylglucan) for target cells (YAC 1, and YAC 1 and K 562 resp.).


Asunto(s)
Adyuvantes Inmunológicos , Glucanos/farmacología , Linfocitos/inmunología , Macrófagos/inmunología , Animales , Citotoxicidad Inmunológica/efectos de los fármacos , Células Asesinas Naturales/efectos de los fármacos , Células Asesinas Naturales/inmunología , Activación de Linfocitos/efectos de los fármacos , Linfocitos/efectos de los fármacos , Macrófagos/efectos de los fármacos , Ratones , Ratones Endogámicos
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