RESUMEN
Osteosarcoma (OSA) is the most common malignant bone cancer in children and dogs. The ther-apeutic protocols adopted for dogs and humans are very similar, involving surgical options suchas amputation. Besides surgical options, radiotherapy and chemotherapy also are adopted. How-ever, hematologic, gastrointestinal and renal toxicity may occur because of chemotherapy treat-ments. Recent study clearly showed that mesenchymal stem cells (MSCs) combined withrecombinant human bone morphogenetic protein (rhBMP-2) may be associated with decreasesof the tumorigenic potential of canine OSA. The aim of this study was to analyse the efficacy ofchemotherapy with carboplatin and rhBMP-2 with MSCs in a canine OSA in vivo model. CanineOSA cells were implanted in mice Balb-c/nude with MSCs, rhBMP-2 and carboplatin. Flow cyto-metry and PCR for markers involved in tumour suppression pathways were analysed. Resultsshowed that the combination of MSCs and rhBMP-2 reduced tumour mass and infiltration ofneoplastic cells in tissues more efficiently than carboplatin alone. Thus it was demonstrated thatthe use of rhBMP-2 and MSCs, in combination with conventional antineoplastic, may be an effi-cient treatment strategy.
RESUMEN
Osteosarcoma (OSA) is the most common malignant bone cancer in children and dogs. The ther-apeutic protocols adopted for dogs and humans are very similar, involving surgical options suchas amputation. Besides surgical options, radiotherapy and chemotherapy also are adopted. How-ever, hematologic, gastrointestinal and renal toxicity may occur because of chemotherapy treat-ments. Recent study clearly showed that mesenchymal stem cells (MSCs) combined withrecombinant human bone morphogenetic protein (rhBMP-2) may be associated with decreasesof the tumorigenic potential of canine OSA. The aim of this study was to analyse the efficacy ofchemotherapy with carboplatin and rhBMP-2 with MSCs in a canine OSA in vivo model. CanineOSA cells were implanted in mice Balb-c/nude with MSCs, rhBMP-2 and carboplatin. Flow cyto-metry and PCR for markers involved in tumour suppression pathways were analysed. Resultsshowed that the combination of MSCs and rhBMP-2 reduced tumour mass and infiltration ofneoplastic cells in tissues more efficiently than carboplatin alone. Thus it was demonstrated thatthe use of rhBMP-2 and MSCs, in combination with conventional antineoplastic, may be an effi-cient treatment strategy.
RESUMEN
A cuíca Gracilinanus microtarsus é um marsupial sul-americano, pertencente à ordem Didelphimorphia e família Didelphidae. Foi estudada a anatomia macroscópica e microscópica dos rins de G. microtarsus. A espécie possui um par de rins localizados na região sublombar da cavidade abdominal adjacente à coluna vertebral, sendo o rim direito mais cranial que o esquerdo. Os rins possuem formato de feijão e ao corte longitudinal possuem áreas bem de nidas como córtex e medula renal, papila, cálice e pelve renal. Em G. microtarsus, o rim é unilobado, apresentando uma superfície lisa e estrutura microscópica similar à de cães e gatos.
Gracilinanus microtarsus is a South American marsupial that belongs to Didelphimorphia order and Didelphidae family. e gross and microscopic anatomy of the kidneys of G. microtarsus was studied. e species has a pair of kidneys located in the sublumbar region of the abdominal cavity, close to the spine. e right kidney is cranial to the le kidney, and both have a bean shape. In a longitudinal cut, the kidney has de ned areas, such as the renal cortex and medulla, as well as the renal papilla, calices and renal pelvis. e kidney of Gracilinanus microtarsus is classi ed as unilobed with a smooth surface and showing microscopic aspect similar to the kidneys of dogs and cats.
