Asunto(s)
Alérgenos/inmunología , Hipersensibilidad Respiratoria/diagnóstico , Hipersensibilidad Respiratoria/inmunología , Adolescente , Adulto , Alérgenos/química , Animales , Pruebas de Provocación Bronquial , Niño , Cricetinae , Femenino , Humanos , Masculino , Persona de Mediana Edad , Phodopus/inmunología , Hipersensibilidad Respiratoria/inducido químicamente , Hipersensibilidad Respiratoria/patología , Pruebas CutáneasRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Femenino , Niño , Adolescente , Adulto Joven , Adulto , Persona de Mediana Edad , Mascotas/inmunología , Phodopus/inmunología , Alergia e Inmunología , Hipersensibilidad/complicaciones , Hipersensibilidad/diagnóstico , Hipersensibilidad/inmunología , Conjuntivitis/complicaciones , Conjuntivitis/inmunología , Conjuntivitis Alérgica/inmunología , Mesocricetus/inmunología , Obstrucción Nasal/complicaciones , Obstrucción Nasal/inmunología , Rinitis/complicaciones , Rinitis/inmunologíaAsunto(s)
Alérgenos/inmunología , Antígenos de Plantas/inmunología , Hipersensibilidad a los Alimentos/diagnóstico , Proteínas de Plantas/inmunología , Salvia/inmunología , Semillas/inmunología , Alérgenos/análisis , Anafilaxia/sangre , Anafilaxia/diagnóstico , Anafilaxia/inmunología , Antígenos de Plantas/análisis , Hipersensibilidad a los Alimentos/sangre , Hipersensibilidad a los Alimentos/inmunología , Humanos , Inmunoglobulina E/sangre , Inmunoglobulina E/inmunología , Masculino , Persona de Mediana Edad , Extractos Vegetales/química , Proteínas de Plantas/análisis , Semillas/químicaRESUMEN
No disponible
Asunto(s)
Humanos , Masculino , Femenino , Semillas/citología , Semillas/enzimología , Rinitis Alérgica Estacional/diagnóstico , Rinitis Alérgica Estacional/metabolismo , Dieta/métodos , Urticaria/patología , Semillas/metabolismo , Rinitis Alérgica Estacional/complicaciones , Dieta , Urticaria/prevención & controlRESUMEN
BACKGROUND: The relevance of contact allergy to plant-related food has recently emerged. Oral allergy syndrome is one of the most characteristic symptoms of fruit allergy, although it also causes systemic reactions. Plant-food allergy is increasing at the same time as pollen allergy, and fruit-induced allergic contact urticaria could be rising as well. OBJECTIVES: The present study was carried out in order to investigate whether one particular primary melon-peel allergen is responsible for contact urticaria. METHODS: Fourteen patients presenting with contact urticaria after touching melon peel were evaluated. A melon-peel extract was prepared and analysed by immunoblotting using the patients' sera. Molecular characterization of IgE-binding bands was performed using mass spectrometry. Melon-peel lipid transfer protein (LTP) was purified. Inhibition studies and contact challenge with the protein were performed to confirm IgE reactivity to the purified allergen. RESULTS: An IgE-binding band of ~8-9 kDa was observed in an immunoblotting assay with all the patients' sera and was identified as an LTP. The melon-peel LTP was purified in two chromatography steps. Inhibition studies confirmed LTP as a major allergen in patients with melon-peel contact urticaria. Contact challenge with melon-peel LTP was performed in five patients, all of whom had positive results, exhibiting itchy erythema and hives in the area of contact. CONCLUSIONS: This study confirmed our previous findings that melon-peel LTP is a major allergen and is responsible for contact allergy. This knowledge may be used to improve both diagnosis and treatment of patients allergic to melon.
