Outer membrane vesicles as nanovaccine candidates against pathogenic Leptospira in experimental Guinea pig model.

Leptospira interrogans serovar Hardjo is a long slender bacterium of size 0.1-0.3 µm × 5-50 µm. It is one of the major causes of bovine leptospirosis and is of economical importance because of the reproductive failure, still birth, abortion, and reduced productivity in cattle. It is also a zoonotic disease-causing infection in humans characterized by headaches, fever, chills, sweats and myalgia, lethargy, aching joints, pulmonary haemorrhages, and death in severe cases. Control of the disease involves antibiotic therapy, management and vaccination, of which immunization is the cheapest and effective means of disease prevention. The present study was developed to isolate and characterize the outer membrane vesicles of Leptospira interrogans serovar Hardjo and to evaluate their vaccine potential in guinea pig model. The OMVs were isolated from the culture by sonication and ultracentrifugation. In transmission electron microscopy, the isolated OMVs appeared as small spherical structures of 50-200 nm size. In Western blot and indirect ELISA, antibodies specific to OMVs were observed as indicative of a good humoral immune response elicited by L. interrogans serovar Hardjo OMV. The OMV-based Leptospira vaccine was able to prevent kidney lesions and renal colonization compared to the control and bacterin vaccinated group as proven by histopathology and PCR.

Optimizing laser cladding powder injection parameters to shape bioactive glass nano-coated zirconium oxide for biomedical application.

Human body is highly sensitive and repairing often incurs pain and expenses. Strength of the materials degraded by poor joint (either weld or link). New material technology is proposed many biomaterials for repairing bone and tissue and also many bio-implantation applications. Especially bioactive material like bioactive glass is used for biomedical applications for replacement and repairing organs in human body. This research work focuses on suggesting material of S53P4 bioactive glass Nano-coated Zirconium dioxide for manufacturing artificial knee implant for fixing in human body. The substrate of Zirconium dioxide is Nano-coated with S53P4 bioactive glass by means of laser cladding process. The laser cladding process parameters were optimized by Taguchi method to enhance mechanical properties like compressive strength, wear resistance and microhardness of Zirconium dioxide implant material. The key parameters like Laser Power (1 kW, 2 kW, 3 kW and 4 kW), beam diameter (2 mm, 3 mm, 4 mm and 5 mm), powder feed rate (10 g/min, 15 g/min, 20 g/min and 25 g/min) and scanning speed (3 mm/s, 4 mm/s, 5 mm/s and 6 mm/s) were considered. The optimal parameters result the higher compressive strength and microhardness are obtained as 373 MPa and 898.37 HV0.2 and minimum wear volume is attained as 0.148 mm3 in the Nano-coated implant material.

Triamcinolone Acetonide Produced by Bacillus velezensis YEBBR6 Exerts Antagonistic Activity Against Fusarium oxysporum f. sp. Cubense: A Computational Analysis.

Fusarium oxysporum f. sp. cubense is one of the most severe and threatening pathogens of bananas, causing "Panama wilt" worldwide. Confrontation assay of Foc antagonistic bacterial endophyte, Bacillus velezensis YEBBR6, with the Foc and GC-MS profiling of excised agar from the zone of inhibition, led to the unveiling of secondary metabolites produced by the endophyte. To refine the probable antifungal compounds among the numerous biomolecules formed during their di-trophic interaction with the pathogen, fungal protein targets were modeled, and docking studies (AutoDock Vina module of the PyRx 0.8 server) were done with all the compounds. Triamcinolone acetonide exhibited the most excellent affinity for the protein targets among the compounds studied. It had a maximum binding affinity of 11.2 kcal/mol for XRN2 (5' → 3'). Further, the protein-ligand complex formation kinetics was done through Molecular Dynamic Simulation studies. Graphs for the RMSD, RMSF, Rg, potential energy, and SASA were generated, and the values during the simulation period suggested the stability of the biomolecule as a complex with the protein. This indicated Triamcinolone acetonide's potential ability to act as a functional disrupter of the target protein and likely an antifungal molecule. Further, the biomolecule was tested for its activity against Foc by screening in the wet lab through the poisoned plate technique, and it was found to be fully inhibitory to the growth of the pathogen at 1000 ppm.

Sustainable wastewater treatment by RO and hybrid organic polyamide membrane nanofiltration system for clean environment.

One of the environmental pollution is happened by the discharge of industrial wastewater that needs to be adequately filtered. Given that the effluent from the leather industry contains high levels of chromium, heavy metals, lipids, and Sulphur, it is one of the wastewater disposals that are most damaging. This experimental study focuses on reverse osmosis and hybrid organic polyimide membrane for nanofiltration for sustainable wastewater treatment. In the RO and organic polyamide Nano-porous membranes, a thin film of polyamide membrane was used for efficient filtration. Taguchi analysis optimized process parameters such as pressure, temperature, pH, and volume reduction factor. The outcome shows an 89% reduction in total wastewater hardness, an 88% reduction in sulfate, and an 89% efficiency reduction in COD. As a result, the proposed technology significantly increased filtration efficiency.

A novel investigations on medical and non-medical mask performance with influence of marine waste microplastics (polypropylene).

The entire human race is struggling with the spread of COVID-19. Worldwide, the wearing of face masks is indispensable to prevent such spread. Despite numerous studies reporting on the fabrication of face masks and surgical masks to reduce spread and thus human deaths, this novel work is considered the marine waste of microplastics, namely Polypropylene (PP) polymer, used to fabricate non-woven fabric masks through the melt-blown process. This experimental work aims to maximize the mask's quality and minimize its fabrication cost by optimizing the melt-blown process parameters and using microplastics. The melt-blown process was used to make masks. Parameters such as extruder temperature, hot air temperature, melt flow rate, and die-to-collector distance (DCD) were investigated as independent variables. The quality of the mask was investigated in terms of bacterial filtration efficiency (BFE), particle filtration efficiency (PFE), and differential pressure. The Taguchi L16 orthogonal array and Taguchi analysis were employed for experimental design and statistical optimization, respectively. The results reveal that the higher BFE and PFE are recorded at 96.7 % and 98.6 %, respectively. The surface morphological investigation on different layers ensured the fine and uniform porosity of the layers and exhibited minimum breath resistance (a low differential pressure of 0.00152 kPa/cm2). Hence the chemically treated marine waste microplastics improved the masks' performance.

AZ63/Ti/Zr Nanocomposite for Bone-Related Biomedical Applications.

Considering the unique properties of magnesium and its alloy, it has a vast demand in biomedical applications, particularly the implant material in tissue engineering due to its biodegradability. But the fixing spares must hold such implants till the end of the biodegradation of implant material. The composite technology will offer the added benefits of altering the material properties to match the requirements of the desired applications. Hence, this experimental investigation is aimed at developing a composite material for manufacturing fixing spares like a screw for implants in biomedical applications. The matrix of AZ63 magnesium alloy is reinforced with nanoparticles of zirconium (Zr) and titanium (Ti) through the stir casting-type synthesis method. The samples were prepared with equal contributions of zirconium (Zr) and titanium (Ti) nanoparticles in the total reinforcement percentage (3%, 6%, 9%, and 12%). The corrosive and tribological studies were done. In the corrosive study, the process parameters like NaCl concentration, pH value, and exposure time were varied at three levels. In the wear study, the applied Load, speed of sliding, and the distance of the slide were considered at four levels. Taguchi analysis was employed in this investigation to optimize the reinforcement and independent factors to minimize the wear and corrosive losses. The minimum wear rate was achieved in the 12% reinforced sample with the input factor levels of 60 N of load on the pin, 1 m/s of disc speed at a sliding distance was 1500 m, and the 12% reinforce samples also recorded a minimum corrosive rate of 0.0076 mm/year at the operating environment of 5% NaCl-concentrated solution with the pH value of 9 for 24 hrs of exposure. The prediction model was developed based on the experimental results.

Infectious complications in compound mandibular fractures undergoing a delayed surgical intervention - a prospective observational study.

The aim of this prospective study was to analyse if a delay in the time from injury to definitive surgical intervention of open reduction and internal fixation (ORIF) of compound mandibular fractures predisposed to an increase in postoperative infectious complications. ORIF beyond 72 hours from injury was considered to be delayed intervention. Postoperative surgical site infections (SSI) and non-infectious complications (NIC) were recorded. The Mann-Whitney U test was used to compare the delay in ORIF with SSI. The chi squared test/Fisher's exact test was used to find the association of the infectious complication status with predetermined risk factors. Eighty-three patients underwent a delayed ORIF with a median (range) of 8 (4-19) days. SSI was documented in eight patients (9.6%) and could be managed as outpatient medical and surgical intervention. Two patients needed repeat surgical intervention due to non-union of the fracture. The median (range) time to ORIF was 6.5 (5-12) days in patients who developed SSI; the Mann-Whitney U test did not show a statistically significant association between delayed ORIF and SSI (p = 0.7). The univariate analysis did not establish a significant relationship between SSI and predetermined risk factors. The delay to definitive surgical intervention was not observed to be an independent attributing factor in postoperative infectious complications of compound mandibular fractures.

The First Differentiated TB Care Model From India: Delays and Predictors of Losses in the Care Cascade.

To reduce TB deaths in resource-limited settings, a differentiated care strategy can be used to triage patients with high risk of severe illness (i.e., those with very severe undernutrition, respiratory insufficiency, or inability to stand without support) at diagnosis and refer them for comprehensive assessment and inpatient care. Globally, there are few examples of implementing this type of strategy in routine program settings. Beginning in April 2022, the Indian state of Tamil Nadu implemented a differentiated care strategy called Tamil Nadu-Kasanoi Erappila Thittam (TN-KET) for all adults aged 15 years and older with drug-susceptible TB notified by public facilities. Before evaluating the impact on TB deaths, we sought to understand the retention and delays in the care cascade as well as predictors of losses. During April-June 2022, 14,961 TB patients were notified and 11,599 (78%) were triaged. Of those triaged, 1,509 (13%) were at high risk of severe illness; of these, 1,128 (75%) were comprehensively assessed at a nodal inpatient care facility. Of 993 confirmed as severely ill, 909 (92%) were admitted, with 8% unfavorable admission outcomes (4% deaths). Median admission duration was 4 days. From diagnosis, the median delay in triaging and admission of severely ill patients was 1 day each. Likelihood of triaging decreased for people with extrapulmonary TB, those diagnosed in high-notification districts or teaching hospitals, and those transferred out of district. Predictors of not being comprehensively assessed included: aged 25-34 years, able to stand without support, and diagnosis at a primary or secondary-level facility. Inability to stand without support was a predictor of unfavorable admission outcomes. To conclude, the first quarter of implementation suggests that TN-KET was feasible to implement but could be improved by addressing predictors of losses in the care cascade and increasing admission duration.

Environmental remediation at vegetable marketplaces through production of biowaste catalysts for biofuel generation.

Large quantities of vegetable biowaste are generated at marketplaces, usually in highly populated locations. On the other hand, nearby markets, hotels, and street shops generate much cooking oil waste and dispose of them in the sewage. Environmental remediation is mandatory at these places. Hence, this experimental work concentrated on preparing biodiesel using green plant wastes and cooking oil. Biowaste catalysts were produced from vegetable wastes and biofuel generated from waste cooking oil using biowaste catalysts to support diesel demand and Environmental remediation. Other organic plant wastes such as bagasse, papaya stem, banana peduncle and moringa oleifera are used as heterogeneous catalysts of this research work. Initially, the plant wastes are independently considered for the catalyst for biodiesel production; secondary, all plant wastes are mixed to form a single catalyst and used to prepare the biodiesel. In the maximum biodiesel yield analysis, the calcination temperature, reaction temperature, methanol/oil ratio, catalyst loading and mixing speed were considered to control the biodiesel production. The results reveal that the catalyst loading of 4.5 wt% with mixed plant waste catalyst offered a maximum biodiesel yield of 95%.

--Eleven tips for operational researchers working with health programmes: our experience based on implementing differentiated tuberculosis care in south India.

Due to the workload and lack of a critical mass of trained operational researchers within their ranks, health systems and programmes may not be able to dedicate sufficient time to conducting operational research (OR). Hence, they may need the technical support of operational researchers from research/academic organisations. Additionally, there is a knowledge gap regarding implementing differentiated tuberculosis (TB) care in programme settings. In this 'how we did it' paper, we share our experience of implementing a differentiated TB care model along with an inbuilt OR component in Tamil Nadu, a southern state in India. This was a health system initiative through a collaboration of the State TB cell with the Indian Council of Medical Research institutes and the World Health Organisation country office in India. The learnings are in the form of eleven tips: four broad principles (OR on priority areas and make it a health system initiative, implement simple and holistic ideas, embed OR within routine programme settings, aim for long-term engagement), four related to strategic planning (big team of investigators, joint leadership, decentralised decision-making, working in advance) and three about implementation planning (conducting pilots, smart use of e-tools and operational research publications at frequent intervals). These may act as a guide for other Indian states, high TB burden countries that want to implement differentiated care, and for operational researchers in providing technical assistance for strengthening implementation and conducting OR in health systems and programmes (TB or other health programmes). Following these tips may increase the chances of i) an enriching engagement, ii) policy/practice change, and iii) sustainable implementation.

Promising strategies of circular bioeconomy using heavy metal phytoremediated plants - A critical review.

Phytoremediation is an excellent method for removing harmful heavy metals from the environment since it is eco-friendly, uses little energy, and is inexpensive. However, as phytoremediated plants can turn into secondary sources for heavy metals, complete heavy metal removal from phytoremediated plants is necessary. Elimination of toxic heavy metals from phytoremediated plants should be considered with foremost care. This review highlights about important sources of heavy metal contamination, health effects caused by heavy metal contamination and technological breakthroughs of phytoremediation. This review critically emphasis about promising strategies to be engaged for absolute reutilization of heavy metals and spectacular approaches of production of commercially imperative products from phytoremediated plants through circular bioeconomy with key barriers. Thus, phytoremediation combined with circular bioeconomy can create a new platform for the eco-friendly life.

Magnetic Co/CoOx@NCNT catalysts for activation of potassium peroxymonosulfate to deteriorate phenol from wastewater.

Phenols are of much toxicological and they must be effectively removed from the wastewater from industries as well as sewage treatment. Such removal demands a special and strong composite. So, this piece of research aims to activate Potassium peroxymonosulfate (PPMS) with the large surface area of magnetite nitrogen-fixed porous carbon nanotube composites (Co/CoOx@NCNT). Increases in the graphitization degree and structural control brought about by the incorporation of reduced Graphite oxide (rGO) significantly increased the catalyst activity of Co/CoOx@NCNT. It was found that PPMS activation for phenol removal by Co/CoOx@NCNT was nearly as effective as by homogeneous Co2+, with nearly 100% removal efficiency in 10 min. Both high reusability and high recycling of Co/CoOx@NCNT were accomplished simultaneously by proving the technology of viability in practical applications. The PPMS activation mechanism in the Co/CoOx@NCNT/PPMS system was driven by the electron transmission from contaminants to PPMS through the sp2- hybrid carbon nanotubes and nitrogen system. The selectivity of the Co/CoOx@NCNT/PPMS system to remove diverse organic compounds was determined by batch experiments. Due to the insignificant impact of radicals reactive on pollutant breakdown, the ability to inhibit species (such as Cl- and natural organic materials) from a minor role was significantly decreased. These results not only shed light on the process of PPMS heterogeneous activation but also provided a framework for the balanced project of highly effective nanocarbon-based catalysts for PPMS activation.

Magnetic porous Ag2O/Chitin nanostructure adsorbent for eco-friendly effective disposing azo dyes.

Water treatment is as much important as it is to satisfying 11 worldwide sustainable development goals out of 17. The removal of Azo is much important as they are toxic and their existence in water, air and food can easily affect humans by triggering allergies, forming tumours etc. Azo contained Dyes Production was banned in many countries. This research aims to synthesize composite Nanorods and Nanospheres and characterize and test to remove Azo dyes from the wastewater. This research used a previously reported method to rapidly synthesize chitin magnetite nanocomposites (ChM) by co-precipitation while irradiating with ultrasound (US). Detailed structural characterization of ChM revealed a crystalline phase analogous to magnetite and spherical morphologies; extending the reaction time to 8 min yielded a "nanorod" type morphology. Both the morphologies displayed a nanoscale limit with particles averaging between 5 and 30 nm in size, resulting the superparamagnetic performance and saturation magnetization values between 45 and 58 emu/g. The nitrogen adsorption-desorption isotherms showed that the surface modification of ChMs resulted in a rise of specific surface area and pore size. Anionic azo dyes (methyl orange (MO) and reactive black 5 (RB5)) adsorption on the surface of nanocomposites was also demonstrated to be pH-dependent, with the reaction favoured for surface-modified samples at pH 4 and unmodified samples at pH 8. Adsorption capacity studies showed that molecule size effect and electrostatic attraction were two distinct adsorption processes for unmodified and modified ChMs. Chitin Magnetite nanoparticles appear to be a substitute for traditional anionic dye adsorbents. Additionally, the two key materials sources, chitin, and magnetite are inexpensive and easily accessible.

An Unusual Case of Recurrent Raised Intracranial Pressure Headache.

INTRODUCTION: Raised intracranial pressure (ICP) can be due to varied etiology. Differentiating among these various etiologies is crucial in making appropriate therapeutic decisions. A patient with a known past history of the primary or secondary headache of any etiology, when presenting with new onset severe headache, needs to be evaluated with imaging to rule out an alternative diagnosis. DISCUSSION: Here, we describe the case details of a young lady who presented with recurrent raised ICP headaches due to three different etiologies. At her third visit, isolated intracranial hypertension (IH) was the only manifestation of cerebral venous sinus thrombosis (CVST), which could have been missed if a repeat magnetic resonance imaging (MRI) brain and venogram were not done. CONCLUSION: Our case highlights the importance of having a high degree of suspicion for CVST in the clinical setting of raised ICP headache in view of its crucial therapeutic implications.

AMAN with Ophthalmoparesis: A Rare Presentation.

Acute motor axonal neuropathy (AMAN) is a variant of Guillain-Barré syndrome (GBS), characterized by acute areflexic flaccid quadriparesis with motor axonal changes and absence of demyelinating findings in electrophysiological studies. A 30-year-old man presented with acute onset flaccid type of weakness involving all four limbs, along with drooping of eyelids. Examination revealed ptosis with restricted horizontal and vertical eye movements. Spinomotor system examination revealed acute flaccid areflexic quadriparesis. Nerve conduction studies (NCS) showed features suggestive of motor axonal neuropathy changes. Cerebrospinal fluid (CSF) revealed albuminocytological dissociation. The diagnosis of AMAN was made, and the patient was treated with intravenous immunoglobulin (IVIg). His weakness gradually improved over 1 month, with partial improvement in ptosis and eye movements. This case highlights the occurrence of ophthalmoparesis in the AMAN variant of GBS. The presence of ophthalmoparesis and areflexia makes it necessary to exclude Miller-Fisher syndrome. But, the presence of axonal changes in nerve conduction study and the profound weakness with negative serum anti-GQ1b antibody profile, supports the diagnosis of AMAN. How to cite this article: Budumuru U, Muralidharan K, Sowmini PR, et al. AMAN with Ophthalmoparesis: A Rare Presentation. J Assoc Physicians India 2023;71(11):103-104.

Comparison of the effectiveness of red ginseng herbal mouth rinse with chlorhexidine and saline in oral cancer patients: A pilot double-blinded randomized control trial.

Background: Red ginseng is an herb with many medicinal properties and aids as a mouth rinse with fewer side effects than chlorhexidine. Aim: The study aimed to compare the efficacy of red ginseng herbal mouth rinses with those of chlorhexidine and saline in oral cancer patients. Materials and Methods: The present pilot study was a double-blinded randomized control trial with 45 histopathologically diagnosed oral squamous cell carcinoma patients divided into three groups: two intervention groups (herbal and chlorhexidine mouth rinse) and one control group (saline). Saliva samples for each patient were collected at baseline and after 14 days of using the mouth rinses. A microbiological examination of salivary samples was done by analysing total oral bacterial load along with specific counts for Porphyromonas gingivalis and Fusobacterium nucleatum at baseline and after the usage of mouth rinse. Statistical Analysis: The data normality was analysed using the Shapiro-Wilk test, and following the normal distribution of data, parametric tests were employed. Paired t-test and one-way analysis of variance, followed by post hoc Bonferroni test, were used for inter-group and intra-group differences. Result: There was a significant mean difference in total colony count, Fusobacterium nucleatum, and Porphyromonas gingivalis with oral hygiene index and gingival index improvement in the red ginseng herbal mouth rinse group when compared to the chlorhexidine and saline groups. Conclusion: In this study, red ginseng mouth rinse exhibited an increased antibacterial effect compared to chlorhexidine and saline. Hence, red ginseng mouth rinse can be used in oral cancer patients to maintain oral health, thereby improving the prognosis of these patients.

Promoting a well-dispersion of MoO3 nanoparticles on fibrous silica catalyst via one-pot synthesis for enhanced photoredox environmental pollutants efficiency.

The coexistence of pharmaceutical compounds and heavy metals in the aquatic environment has resulted in complications in the treatment process and thus, causing uproar among the citizens. The radical-based photocatalysis technology has aroused as an excellent method to eliminate both heavy metal and pharmaceutical compounds in the water. Herein, reported the utilization of the microemulsion technique for the preparation of nanoporous fibrous silica-molybdenum oxide (FSMo) towards simultaneous photocatalytic abatement of hexavalent chromium (Cr(VI)) and tetracycline (TC). The FESEM analysis showed the spherical morphology of the FSMo catalyst with dendrimeric silica fiber. The synthesized FSMo catalyst exhibited narrowed bandgap, high crystallinity, and well Mo element dispersion for enhanced photo-redox of Cr(VI) and TC. Remarkably, simultaneous remediation of the Cr(VI) and TC over FSMo demonstrated superior photocatalytic efficiency, 69% and 75%, respectively, than in the individual system, possibly due to the effective separation of photoinduced charges. The introduction of the Mo element to the silica framework via microemulsion technique demonstrated better dispersion of Mo compared to the incipient wetness impregnation method and thus, yielded higher photocatalytic activity towards simultaneous removal of TC and Cr(VI). Besides, quenching experiments revealed the electrons and holes as the active species that play a dominant role in the simultaneous photo-redox of Cr(VI) and TC. Lastly, the FSMo catalyst demonstrated high stability after four continuous cycles of simultaneous photocatalysis reactions, implying its potential as a suitable material for practical wastewater treatments.