Surface-initiated polymerization of PVDF membrane using amine and bismuth tungstate (BWO) modified MIL-100(Fe) nanofillers for pesticide photodegradation.

Pirimicarb as a pesticide is used to control the aphids in the agriculture field; however, it affects the groundwater ecosystem by leaching through the soil profile. The post-synthetic amine and BWO modified MIL-100 (Fe) nanofillers were synthesized. The photocatalytic property of amine-functionalized and BWO@MIL-100(Fe) nanofillers was confirmed by the lesser bandgap energy than the unmodified MIL-100 (Fe) nanofiller. Herein, we constructed a nanofillers grafted PVDF membrane via in-situ polymerization technique for the pirimicarb reduction and photodegradation. Furthermore, the nanofiller's grafted membranes were characterized by FESEM, XRD, FTIR, and contact angle analysis. The carboxylic acid peak was observed on the FTIR which demonstrated the PAA grafted on the membrane surface and similar crystalline peaks evident that the nanofillers were grafted on the membrane surface. Furthermore, surface morphology studies have exhibited the dispersion of nanofillers and enhanced microvoids in the cross-section of the membrane. The decrease in the water contact angle of the membrane depicted the improved antifouling properties and surface energy. The nanofiller's grafted membranes have shown higher hydrophilicity correlated well with the enhanced pure water flux in the order M4 > M5 > M2 > M3 > M6 > M7 compared to the neat membrane (M1). In BWO@MIL-100(Fe) membrane has shown a higher permeate flux (25.99 L m-2.h-1) than the neat PVDF membrane. The BWO@MIL-100(Fe) grafted PVDF membrane has also shown excellent pirimicarb photodegradation of 81% at pH 5. The proposed MIL-100 (Fe) and bismuth tungsten nanocomposite will pave the way for the different MOF-based photocatalytic materials for membrane-based pesticide degradation.

Detection of plant-based adulterants in turmeric powder using DNA barcoding.

CONTEXT: In its powdered form, turmeric [Curcuma longa L. (Zingiberaceae)], a spice of medical importance, is often adulterated lowering its quality. OBJECTIVE: The study sought to detect plant-based adulterants in traded turmeric powder using DNA barcoding. MATERIALS AND METHODS: Accessions of Curcuma longa L., Curcuma zedoaria Rosc. (Zingiberaceae), and cassava starch served as reference samples. Three barcoding loci, namely ITS, rbcL, and matK, were used for PCR amplification of the reference samples and commercial samples representing 10 different companies. PCR success rate, sequencing efficiency, occurrence of SNPs, and BLAST analysis were used to assess the potential of the barcoding loci in authenticating the traded samples of turmeric. RESULTS: The PCR and sequencing success of the loci rbcL and ITS were found to be 100%, whereas matK showed no amplification. ITS proved to be the ideal locus because it showed greater variability than rbcL in discriminating the Curcuma species. The presence of C. zedoaria could be detected in one of the samples whereas cassava starch, wheat, barley, and rye in other two samples although the label claimed nothing other than turmeric powder in the samples. DISCUSSION AND CONCLUSION: Unlabeled materials in turmeric powder are considered as adulterants or fillers, added to increase the bulk weight and starch content of the commodity for economic gains. These adulterants pose potential health hazards to consumers who are allergic to these plants, lowering the product's medicinal value and belying the claim that the product is gluten free. The study proved DNA barcoding as an efficient tool for testing the integrity and the authenticity of commercial products of turmeric.

A simple and efficient protocol for isolation of high quality functional RNA from different tissues of turmeric (Curcuma longa L.).

Many experiments in plant molecular biology require processing of a large number of RNA samples and in some cases large quantities are required for a single application. In turmeric, a major spice and medicinal plant, a protocol for RNA isolation is not available. The major difficulty encountered while using other popular protocols is the low yield and quality of RNA which hampers the downstream applications like qRT-PCR, cDNA synthesis and micro RNA isolation. Commercial kits though available are costly and were found to be unsuccessful in case of rhizomes and root tissues that are rich in polyphenols, polysaccharides and alkaloids. It was thus felt that a quick, handy and cheap protocol of total RNA isolation from different tissues of turmeric was required for day to day working in our lab. The new protocol utilizes SDS based extraction buffer including ß-mercaptoethanol and PVP with sequential acid phenol:chloroform extraction to remove polyphenols and proteins, followed by the purification with sodium acetate to eliminate polysaccharides. The protocol is simple and can be completed in less than 3 h. The RNA yield from rhizome was higher by more than fivefold with both A260/280 and A260/230 ratio in the range of 1.8-2.0. The protocol worked well with leaf, rhizome, pseudostem and root tissues with RIN >7.0 and the isolated RNA could be successfully used for cDNA synthesis, RT-PCR, qRT-PCR and small RNA isolation including microRNA.

Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).

Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.

Isolation and amplification of genomic DNA from recalcitrant dried berries of black pepper (Piper nigrum L.)--a medicinal spice.

Black pepper is an important medicinal spice traded internationally. The extraction of high quality genomic DNA for PCR amplification from dried black pepper is challenging because of the presence of the exceptionally large amount of oxidized polyphenolic compounds, polysaccharides and other secondary metabolites. Here we report a modified hexadecyl trimethyl ammonium bromide (CTAB) protocol by incorporating potassium acetate and a final PEG precipitation step to isolate PCR amplifiable genomic DNA from dried and powdered berries of black pepper. The protocol has trade implication as it will help in the PCR characterization of traded black peppers from different countries.

Creatine kinase and its isoenzyme MB in patients after open heart surgery.

Total serum creatine kinase (CK) and its isoenzyme MB (CKMB) were measured before and 4, 24, 48 and 72 hours after termination of cardiopulmonary bypass in patients undergoing (I) atriotomy, (II) ventriculotomy and (III) coronary artery bypass surgery. All patients were free of postoperative complications and myocardial infarction as defined by clinical course, 12 lead ECG and 2D echocardiography. Peak elevation of CK occurred at 24th hour and CKMB at 4th hour and then gradually declined. There was no relation between the peak level of rise of CK or CKMB with cross clamp time or bypass time. The 96th percentile values of absolute CKMB level at 4, 24, 48 and 72 hours may suggest perioperative myocardial infarction with specificity of 95%. In addition, the rising value of CKMB beyond 24 hours after the termination of bypass may also suggest occurrence of myocardial infarction.