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1.
Mater Sci Eng C Mater Biol Appl ; 119: 111552, 2021 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-33321616

RESUMEN

Nanocellulose pellicle is produced as a byproduct during the symbiotic culture of bacteria and yeast in kombucha. It shows good mechanical strength, biocompatibility and hydrophilicity. However, it has limited application in tissue engineering due to its low processability. In this work, bacterial cellulose-based sustainable kombucha (KBC) sheet has been produced and it was acid-treated to partially hydrolyse. This controlled process improves its extrusion and shape formation ability. The physical, functional and biological properties were studied to assess its potential as a 3D printed scaffold. Two different cell lines (Human dermal fibroblast cells and mouse osteoblast cells) were used to study the cytocompatibility. Both the cell types showed good attachment, growth and proliferation on the pure and treated KBC. They attained almost full confluence within 3 days. This study indicates that the controlled partial hydrolysis of KBC can make it suitable for 3D printing retaining its mechanical strength and cytocompatibility. This sustainable microbial biopolymer shows the possibility to be used as a bioink for 3D bioprinting.


Asunto(s)
Bioimpresión , Celulosa , Impresión Tridimensional , Ingeniería de Tejidos , Andamios del Tejido
3.
Artif Cells Nanomed Biotechnol ; 46(3): 589-598, 2018 May.
Artículo en Inglés | MEDLINE | ID: mdl-28554257

RESUMEN

In the present study, a sustainable green chemistry approach was established to fabricate magnetic Fe3O4 nanoparticles (Fe3O4NPs) using the aqueous fruit extract of edible C. guianensis (CGFE). Synthesized NPs were further confirmed with different high-throughput characterization techniques such as UV-visible spectroscopy, FT-IR, XPS, DLS and zeta potential analysis. Additionally, XRD, AFM, HRTEM and SQUID VSM demonstrate the generation of crystalline CGFe3O4NPs with mean diameter of 17 ± 10 nm. Interestingly, CGFe3O4NPs exhibit a stupendous bactericidal action against different human pathogens which depicts its antimicrobial value. A significant dose-dependent cytotoxic effect of CGFe3O4NPs was noticed against treated human hepatocellular carcinoma cells (HepG2).


Asunto(s)
Antibacterianos , Carcinoma Hepatocelular/tratamiento farmacológico , Citotoxinas , Frutas/química , Lecythidaceae/química , Neoplasias Hepáticas/tratamiento farmacológico , Nanopartículas de Magnetita , Extractos Vegetales/química , Antibacterianos/síntesis química , Antibacterianos/química , Antibacterianos/farmacología , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patología , Citotoxinas/síntesis química , Citotoxinas/química , Citotoxinas/farmacología , Células Hep G2 , Humanos , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patología , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/uso terapéutico
4.
ACS Appl Mater Interfaces ; 8(6): 4086-100, 2016 Feb 17.
Artículo en Inglés | MEDLINE | ID: mdl-26799576

RESUMEN

Guided bone regeneration (GBR) scaffolds are futile in many clinical applications due to infection problems. In this work, we fabricated GBR with an anti-infective scaffold by ornamenting 2D single crystalline bismuth-doped nanohydroxyapatite (Bi-nHA) rods onto segmented polyurethane (SPU). Bi-nHA with high aspect ratio was prepared without any templates. Subsequently, it was introduced into an unprecedented synthesized SPU matrix based on dual soft segments (PCL-b-PDMS) of poly(ε-caprolactone) (PCL) and poly(dimethylsiloxane) (PDMS), by an in situ technique followed by electrospinning to fabricate scaffolds. For comparison, undoped pristine nHA rods were also ornamented into it. The enzymatic ring-opening polymerization technique was adapted to synthesize soft segments of PCL-b-PDMS copolymers of SPU. Structure elucidation of the synthesized polymers is done by nuclear magnetic resonance spectroscopy. Sparingly, Bi-nHA ornamented scaffolds exhibit tremendous improvement (155%) in the mechanical properties with excellent antimicrobial activity against various human pathogens. After confirmation of high osteoconductivity, improved biodegradation, and excellent biocompatibility against osteoblast cells (in vitro), the scaffolds were implanted in rabbits by subcutaneous and intraosseous (tibial) sites. Various histological sections reveal the signatures of early cartilage formation, endochondral ossification, and rapid bone healing at 4 weeks of the critical defects filled with ornamented scaffold compared to SPU scaffold. This implies osteogenic potential and ability to provide an adequate biomimetic microenvironment for mineralization for GBR of the scaffolds. Organ toxicity studies further confirm that no tissue architecture abnormalities were observed in hepatic, cardiac, and renal tissue sections. This finding manifests the feasibility of fabricating a mechanically adequate nanofibrous SPU scaffold by a biomimetic strategy and the advantages of Bi-nHA ornamentation in promoting osteoblast phenotype progression with microbial protection (on-demand) for GBR applications.


Asunto(s)
Antiinfecciosos , Infecciones Bacterianas/tratamiento farmacológico , Bismuto , Regeneración Ósea/efectos de los fármacos , Condrogénesis/efectos de los fármacos , Durapatita , Andamios del Tejido/química , Animales , Antiinfecciosos/química , Antiinfecciosos/farmacología , Bismuto/química , Bismuto/farmacología , Dimetilpolisiloxanos/química , Dimetilpolisiloxanos/farmacología , Durapatita/química , Durapatita/farmacología , Humanos , Masculino , Poliésteres/química , Poliésteres/farmacología , Conejos
5.
Spectrochim Acta A Mol Biomol Spectrosc ; 138: 885-90, 2015 Mar 05.
Artículo en Inglés | MEDLINE | ID: mdl-25467657

RESUMEN

The present study reports, green synthesis of bioactive silver nanoparticles (AgNPs) under different temperature (60°C, room temperature and 4° refrigerator) using the aqueous extract of sea grass Cymodocea serrulata as a potential bioreductant. Increased temperature fabricates more AgNPs compare to room temperature and refrigerator condition. At first the reduction of Ag(+) ions were confirmed through color change which produces an absorbance spectra at 420nm in UV-Visible spectrophotometer. Additionally various exclusive instrumentations such as X-ray diffraction (XRD), Dynamic light scattering (DLS), scanning electron microscope (SEM) analysis and Transmission electron microscope (TEM) were authorizes the biosynthesis and physio-chemical characterization of AgNPs. From Fourier transform infrared spectroscopy (FTIR) analysis, it was identified that the water soluble fractions of the sea grass mainly responsible for reduction of ionic silver (Ag(+)) into (Ag(0)) nano-ranged particles and also they act as stabilizing agent to sustain the durability of NPs for long period of time. Further, synthesized AgNPs shows potential cytotoxicity against human lung cancer A549 cells (LD50-100µg/ml). The overall results suggest that C. serrulata is a valuable bioresource to generate rapid and eco-friendly bioactive AgNPs towards cancer therapy.


Asunto(s)
Nanopartículas del Metal/toxicidad , Tamaño de la Partícula , Poaceae/química , Plata/toxicidad , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Humanos , Concentración 50 Inhibidora , Nanopartículas del Metal/ultraestructura , Extractos Vegetales/química , Hojas de la Planta/química , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos X
6.
Colloids Surf B Biointerfaces ; 106: 86-92, 2013 Jun 01.
Artículo en Inglés | MEDLINE | ID: mdl-23434696

RESUMEN

A generation of nanoparticles research has discussed recently. It is mandatory to elaborate the applications of biogenic nanoparticles in general and anticancereous property in particular. The present study was aimed to investigate the in vitro cytotoxicity effect of biogenic silver nanoparticles (AgNPs) against human breast cancer (MCF-7) cells towards the development of anticancer agent. Biogenic AgNPs were achieved by employing Sesbania grandiflora leaf extract as a novel reducing agent. It was well characterized by FESEM, EDAX and spectral studies showed spherical shaped nanoparticles in the size of 22 nm in slightly agglomerated form. It was surprising that biogenic AgNPs showed cytotoxic effect against MCF-7 cell lines were confirmed by MTT, AO-EB, Hochest and COMET assays. There was an immediate induction of cellular damage in terms of loss of cell membrane integrity, oxidative stress and apoptosis were found in the cell which treated with AgNPs. This may be a first report on anti-MCF-7 property of biogenic AgNPs in the fourth generation of nanoparticles research. It is necessary to study the formulation and clinical trials to establish the nano drug to treat cancer cells.


Asunto(s)
Neoplasias de la Mama/patología , Nanopartículas del Metal , Plata/química , Apoptosis , Neoplasias de la Mama/terapia , Línea Celular Tumoral , Ensayo Cometa , Femenino , Humanos , Microscopía Electrónica de Rastreo , Extractos Vegetales/farmacología , Sesbania/química , Espectrofotometría Ultravioleta
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