Use of plasma-based spectroscopy and infrared microspectroscopy techniques to determine the uptake and effects of chromium(III) and chromium(VI) on Parkinsonia aculeata.

Chromium uptake and tolerance by Mexican Palo Verde (Parkinsonia aculeata) (MPV) was studied in a six-month experiment with Cr(III) and Cr(VI) at 60 and 10 mg kg(-1), respectively. Chromium and nutrient uptake were determined by ICP-OES and changes in macromolecules were studied by infrared microspectroscopy (IMS). In the Cr(VI)-treated plants, chromium concentration increased in the roots only through the third month, while translocation to stems increased constantly throughout the six months. Cr(III) applications decreased the amount of Zn in leaves and stems (p < or = 0.05). Cr(VI) increased P and S in all plant tissues and increased Ca in roots, but decreased Ca in stems and leaves, and Mg in roots and stems. Cr(III) decreased P in stems and leaves, while both Cr ions decreased K in all MPV tissues. Relative to untreated plant tissue, the IMS revealed significant changes at 1730 cm(-1) and 845 cm(-1). Changes at 1730 cm(-1) indicated that the cortex and xylem of Cr-treated plants were more proteinaceous. Changes at 845 cm(-1) revealed higher lignifications in cortex. However, at the stem level, Cr(VI) decreased lignin deposition in xylem. The data showed that MPV could be useful in the phytoremediation of Cr in moderately impacted soils.

Potential of Chilopsis linearis for gold phytomining: using XAS to determine gold reduction and nanoparticle formation within plant tissues.

This study reports on the capability of the desert plant Chilopsis linearis (Cav.) Sweet (desert willow) to uptake gold (Au) from gold-enriched media at different plant-growth stages. Plants were exposed to 20, 40, 80, 160, and 320 mg Au L(-1) in agar-based growing media for 13, 18, 23, and 35 d. The Au content and oxidation state of Au in the plants were determined using an inductively coupled plasma/optical emission spectrometer (ICP/OES) and X-ray absorption spectroscopy (XAS), respectively. Gold concentrations ranging from 20 to 80 mg Au L(-1) did not significantly affect Chilopsis linearis plant growth. The concentration of gold in the plants increased as the age of the plant increased. The Au concentrations in leaves for the 20, 40, 80, and 160 mg Au L(-1) treatments were 32, 60, 62, and 179 mg Au kg(-1) dry weight mass, respectively, demonstrating the gold uptake capability of desert willow. The XAS data indicated that desert willow produced gold nanoparticles within plant tissues. Plants exposed to 160 mg Au L(-1) formed nanoparticles that averaged approximately 8, 35, and 18 A in root, stem, and leaves, respectively. It was observed that the average size of the Au nanoparticles formed by the plants is related to the total Au concentration in tissues and their location in the plant

Using FTIR to corroborate the identity of functional groups involved in the binding of Cd and Cr to saltbush (Atriplex canescens) biomass.

Fourier transform infrared (FTIR) studies were performed to confirm the chemical modification of saltbush (Atriplex canescens) biomass and to provide information about the identity and binding characteristics of the chemical groups responsible for the binding of Cd(II), Cr(III), and Cr(VI). In addition, studies were performed to determine the optimum time for the binding of the three ions by saltbush biomass, and to study the efficiency of HCl and sodium citrate as stripping agents. The metal quantification was performed using inductively coupled plasma optical emission spectroscopy (ICP-OES). The results showed that 10 min or less is enough to achieve the maximum metal binding, and that aqueous solutions of 0.1 mM HCl or sodium citrate were enough to strip more than 80% of the bound Cd. It was determined that more than 70% of the bound Cr(III) was stripped using 0.1 mM HCl. Chemical modification of carboxyl and ester groups on the biomass was performed. The FTIR results confirmed that the esterification of carboxyl groups and hydrolysis of ester groups in the native biomass had occurred. The direct effect of these modifications on the binding properties of the biomass provided strong evidence that the carboxyl functionality is the main group responsible for binding Cd and Cr(III). However, the IR data showed that for Cr(VI), a different type of functional group is involved.