Nanostructured zinc oxide film amalgamated with functionalized carbon nanotubes for facile electrochemical determination of nifedipine.

An innovative approach has been employed for the detection of nifedipine at glassy carbon electrode fabricated with zinc oxide nanoparticles embedded on functionalized multi walled carbon nanotubes. Herein, square wave voltammetry being an expeditious electrochemical technique has been utilized for the first time for the determination of nifedipine. Instrumental variables were altered to acquire optimized operational parameters. The electrochemical oxidation peak of nifedipine was procured at ∼ 807 mV which was recorded versus Ag/AgCl reference electrode. The oxidation peak was used to quantify the analyte in the dynamic linear range of 1 nM to 40 µM with highest sensitivity and lowest detection limit of 21.8 µA µM-1 and 0.49 nM respectively. The influence of common physiological interferents on the current signal of the analyte was examined. Pronounced stability and reproducibility of fabricated sensor was attained by the neoteric electrochemical approach. The developed protocol was efficaciously applied to quantify nifedipine in pharmaceutical formulations. The urine and blood serum sample of patients being treated for hypertension was effectively detected with nifedipine for the first time. The biological sample assay without the interference of the metabolites coexisting in the samples outlined the insight of selectivity of the developed sensor.

Sensitive detection of brucine an anti-metastatic drug for hepatocellular carcinoma at carbon nanotubes - nafion composite based biosensor.

A neoteric approach for the electrochemical analysis of brucine in biological environment has been developed. The glassy carbon electrode modified with single walled carbon nanotubes and nafion composite film is delineated for the first time to determine brucine employing square wave voltammetry. The quantification of brucine at physiological pH 7.2 manifests remarkable performance at the developed biosensor. The effect of several operational parameters has been studied in the present investigation. Under optimized conditions, a dynamic linear range from 1nM to 8µM with a high sensitivity of 340.8µAµM-1 and limit of detection corresponding to 0.11nM was achieved. The interfering effect of some coexisting metabolites on the current response of brucine has been reported. The method was successfully applied for the detection of brucine in traditional pharmaceutical formulations. The biological relevance of the present method has been demonstrated by the analysis of the alkaloid in human serum and urine samples. The analytical utility was further assessed by the selective determination of brucine in Strychnos nux-vomica seeds exhibiting considerable potential as a diagnostic tool.