RESUMEN
An 8-year-old, mixed breed, polled goat was presented for evaluation of male-like behaviour. Clinical findings included clitoromegaly, a heavily muscled neck, pronounced beard, and erect dorsal guard hairs, which are phenotypic characteristics commonly observed in intersex animals. Transrectal ultrasonography revealed the presence of two abdominal masses caudolateral to the uterine horns. Serum concentration of estradiol was elevated. Genetic evaluation was compatible with polled intersex syndrome defined by an XX karyotype without a Y chromosome or SRY gene. Based on gross and histologic evaluation, the abdominal masses were determined to be intra-abdominal testes, each of which was effaced by Sertoli cell and interstitial (Leydig) cell tumours. The Sertoli cell tumours (SCTs) represented two unique histologic patterns. Regardless of pattern, neoplastic Sertoli cells were consistently lipid laden and positive for vimentin. Interstitial cell tumours (ICTs) were negative for vimentin. Clinical and histopathologic findings suggest that prolonged exposure to steroids secreted by neoplastic Sertoli cells contributed to virilization. In addition, results from immunohistochemistry indicated that vimentin may be a valuable immunodiagnostic tool for differentiation between interstitial and Sertoli cell tumours in goats.
Asunto(s)
Trastornos del Desarrollo Sexual/veterinaria , Enfermedades de las Cabras/patología , Tumor de Células de Leydig/veterinaria , Tumor de Células de Sertoli/veterinaria , Neoplasias Testiculares/veterinaria , Animales , Trastornos del Desarrollo Sexual/complicaciones , Trastornos del Desarrollo Sexual/patología , Femenino , Cabras , Tumor de Células de Leydig/complicaciones , Tumor de Células de Leydig/patología , Masculino , Tumor de Células de Sertoli/complicaciones , Tumor de Células de Sertoli/patología , Neoplasias Testiculares/complicaciones , Neoplasias Testiculares/patología , Testículo/patologíaRESUMEN
Lesions most commonly found in the canine and feline uteri fall primarily into two categories: (i) abnormalities of endometrial growth and repair and (ii) uterine infections with associated endometritis. Neoplastic conditions of the tubular genitalia of the bitch, with the possible exception of leiomyomas (tumours that arise from smooth muscle cells), are uncommon. Congenital lesions involving the uterus are relatively rare. The primary object of this paper is to provide a summary of the most common uterine lesions found in the canine and feline uterus using a series of images that underscore the most prominent and important gross diagnostic features of each.
Asunto(s)
Enfermedades de los Gatos/patología , Enfermedades de los Perros/patología , Enfermedades Uterinas/veterinaria , Animales , Biopsia/veterinaria , Enfermedades de los Gatos/diagnóstico , Gatos , Enfermedades de los Perros/diagnóstico , Perros , Femenino , Enfermedades Uterinas/diagnóstico , Enfermedades Uterinas/patologíaRESUMEN
Transcervical endometrial biopsy is a useful tool for obtaining information about uterine health in some species. The clinical application of information gained from histopathological interpretations of endometrial biopsies in the bitch has not been validated. We hypothesized that transcervical endometrial biopsy samples would be as diagnostic as full-thickness uterine sections in identifying cystic endometrial hyperplasia (CEH), inflammation and periglandular fibrosis. Endometrial biopsies were obtained from 20 female adult dogs. Vaginal swabs, gross appearance of the vulva and vaginal tract, and serum progesterone values were used to determine the stage of the oestrous cycle at the time of sampling. The uteri were removed between 1 and 6 days after the biopsy procedure, and full-thickness sections were collected from each uterine horn and ovary and processed for histopathology. Two pathologists, blinded to the origin of each sample, compared full-thickness sections from the excised uteri to the biopsy samples collected via the transcervical technique. Pathologic features noted included: CEH, inflammation and periglandular fibrosis. Pathological diagnoses obtained from the biopsy sections were compared with those obtained from the full-thickness sections, as well as comparing diagnoses between the two pathologists, using McNemar's test. Of the 59 total biopsy samples obtained, 54 were considered diagnostic. All stages of the canine oestrous cycle were represented (anoestrus, proestrus, oestrus and dioestrus). Pyometra was not noted in any of the transcervical biopsy sections, but was noted in many of the full-thickness sections collected from dogs in dioestrus, suggesting either that biopsy is not a sensitive indicator of pyometra or that the procedure may induce pyometra in dioestrous dogs. Transcervical endometrial biopsy showed similar sensitivity as full-thickness sections in detecting CEH, inflammation and fibrosis. No differences in describing lesions were detected between pathologists.
Asunto(s)
Enfermedades de los Perros/diagnóstico , Enfermedades Uterinas/veterinaria , Útero/patología , Animales , Biopsia/veterinaria , Enfermedades de los Perros/patología , Perros , Femenino , Enfermedades Uterinas/diagnóstico , Enfermedades Uterinas/patologíaRESUMEN
A 4-year-old bull mastiff presented due to premature labour. The referring veterinarian elected to perform a caesarian delivery and at the time of surgery a 4×4×2 cm round, smooth, red to tan, lobulated soft mass was identified attached to the allantoic surface of the zonary placenta of one pup. Microscopically, this mass was composed of loosely arranged confluent undulating cords of polygonal to columnar epithelioid cells separated by a fine fibrovascular stroma resembling the placental labyrinth. The labyrinthine structure and epithelioid nature of the cells suggested that the mass was of trophoblastic origin. Due to the non-invasive nature of the mass and relatively low mitotic activity, this proliferative trophoblastic mass was considered to be benign. The absence of morphological features supporting malignant behaviour and the recapitulation of the normal labyrinthine architecture led to the diagnosis of a trophoblastic hamartoma. To the authors' knowledge this is the first report of a placental hamartoma in the dog.
Asunto(s)
Enfermedades de los Perros/patología , Retardo del Crecimiento Fetal/veterinaria , Hamartoma/veterinaria , Trabajo de Parto Prematuro/veterinaria , Enfermedades Placentarias/veterinaria , Placenta/patología , Complicaciones del Embarazo/veterinaria , Animales , Perros , Femenino , Retardo del Crecimiento Fetal/etiología , Hamartoma/diagnóstico , Hamartoma/patología , Índice Mitótico , Trabajo de Parto Prematuro/etiología , Enfermedades Placentarias/diagnóstico , Enfermedades Placentarias/patología , Embarazo , Complicaciones del Embarazo/patología , Trofoblastos/patologíaRESUMEN
Investigation of abnormal sexual development in companion animals can allow for the elimination of inherited disorders from breeding populations while contributing to the understanding of the complex process of mammalian sexual development and differentiation. A 1-year-old mixed-breed cat, presented for neutering, was tentatively diagnosed as a male with bilateral cryptorchidism. During surgery, the surgeon identified gonads in an ovarian position and a complete bicornuate uterus. Both testicular and ovarian architecture in the gonads and Mullerian and Wolffian duct derivatives were identified histologically. The karyotype was that of a normal male (38,XY), and no causative mutation was identified in the feline SRY coding sequence amplified from genomic DNA. All features of the case were compatible with a diagnosis of SRY-positive 38,XY sex reversal, true hermaphrodite phenotype. To the authors' knowledge, this is the first report of this disorder in a domestic cat.
Asunto(s)
Enfermedades de los Gatos/patología , Trastornos Ovotesticulares del Desarrollo Sexual/veterinaria , Proteína de la Región Y Determinante del Sexo/genética , Animales , Enfermedades de los Gatos/genética , Enfermedades de los Gatos/cirugía , Gatos , Femenino , Gónadas/patología , Cariotipificación , Masculino , Trastornos Ovotesticulares del Desarrollo Sexual/genética , Trastornos Ovotesticulares del Desarrollo Sexual/patología , Trastornos Ovotesticulares del Desarrollo Sexual/cirugía , Útero/patologíaRESUMEN
Knowledge of the causes of canine or feline pregnancy loss is limited and the success rate for making a definitive diagnosis is disappointingly low. Although these facts are discouraging, there are some things that can be done to improve success rates. This paper will address limitations and explore ways for improvement. For abortions caused by microbial infections, there are many reasons why it may not possible to identify the agents. "Non-infectious" causes are much more difficult to diagnose, and their relative importance is unknown. These include endocrine failure, underlying endometrial disease, genetic abnormalities, nutritional deficiencies, and toxicosis from drugs or environmental sources. Genetic abnormalities are a major cause of human pregnancy loss, yet we have little specific information about genetic diseases leading to abortion in animals. This paper addresses ways clinicians and diagnosticians can work together to improve diagnostic success. Necropsy techniques for fetal and placental examination and sampling are briefly reviewed. It is hoped that this series of papers will stimulate discussion on the causes and pathogenesis of pregnancy failure, and focus attention on areas where abortion diagnostics can be improved.
Asunto(s)
Aborto Veterinario/diagnóstico , Enfermedades de los Gatos/diagnóstico , Enfermedades de los Perros/diagnóstico , Animales , Gatos , Perros , Femenino , EmbarazoRESUMEN
Cystic lesions in the uteri of bitches and queens arise from the uterine serosa, myometrium, or endometrium and include: serosal inclusion cysts, adenomyosis, endometrial polyps, cystic remnants of mesonephric ducts, and cysts associated with endometrial hyperplasia (both cystic glands and "pseudo-placentational" hyperplasia). Of these, "cystic endometrial hyperplasia (CEH)" is the most common and is frequently associated with pyometra. A second form of endometrial hyperplasia occurs in the bitch; although it was first described over 100 y ago, it is not widely recognized by clinicians or diagnostic pathologists. In this form, the endometrium proliferates in a highly organized manner, remodeling the uterine lining to closely resemble the histology of the endometrium at placentation sites in normal pregnancy. Although this lesion is very different from CEH, it is quite easy to induce in dogs during the luteal phase of their cycles and has been perhaps inappropriately proposed as modeling CEH. This lesion has been referred variously as "deciduoma", endometrial hyperplasia in pseudocyesis, and "maternal placental-like endometrial hyperplasia". An alternative name is suggested that is descriptive and draws attention to the difference between this lesion and CEH; the term pseudo-placentational endometrial hyperplasia (PEH) is proposed. The histopathology and pathogenesis of CEH and PEH are discussed. The objectives of this paper are to review the pathophysiology of cystic lesions of canine uterus, to demonstrate these using subgross photomicrographs taken from natural cases, and to present key diagnostic features of each.
Asunto(s)
Enfermedades de los Gatos/patología , Enfermedades de los Perros/patología , Hiperplasia Endometrial/veterinaria , Envejecimiento , Animales , Gatos , Perros , Hiperplasia Endometrial/patología , FemeninoRESUMEN
During the 1960s and 1970s, the clinical value of equine endometrial histopathology was firmly established after it was shown that fertility outcome was correlated with the presence and severity of specific microscopic lesions. The objective of this paper is to summarize reports from the veterinary literature published after the mid 1980s that describe new diagnostic methods of assessing equine uterine health using material collected by endometrial biopsy.
Asunto(s)
Endometrio/patología , Enfermedades de los Caballos/patología , Enfermedades Uterinas/veterinaria , Animales , Biopsia/historia , Técnicas de Diagnóstico Obstétrico y Ginecológico/historia , Técnicas de Diagnóstico Obstétrico y Ginecológico/veterinaria , Epitelio/patología , Femenino , Historia del Siglo XX , Historia del Siglo XXI , Caballos , Sensibilidad y Especificidad , Enfermedades Uterinas/patologíaRESUMEN
In viviparous animals, regulation of expression of major histocompatibility complex (MHC) class I antigens by the trophoblast cells, which constitute the outermost layer of the placenta, seems to be critical for maternal immunological acceptance of an allogeneic fetus. Cattle are unusual in this regard, since the bovine trophoblast cells, in specific regions of the uterine/placental interface, normally express MHC class I antigens during the third trimester of gestation. This expression appears to be biologically relevant as MHC class I compatibility between a cow and her fetus has been associated with an increased incidence of placental retention. We have found significant differences in lymphocyte populations, cytokine production, and trophoblast cell apoptosis in the placentomes of MHC-compatible and -incompatible pregnancies at parturition. This suggests that maternal immunological recognition of fetal MHC class I proteins triggers an immune/inflammatory response that contributes to placental separation at parturition in cattle. Early in pregnancy, a complete shutdown of MHC class I expression by trophoblast cells appears to be critical for normal placental development and fetal survival. In bovine somatic cell nuclear transfer (SCNT) pregnancies, there is an extremely high rate of fetal loss between days 30 and 90 of pregnancy. We have shown that in bovine SCNT pregnancies, between days 34 and 63 of gestation, there is both abnormal expression of MHC class I antigens by trophoblast cells and an abnormal accumulation of lymphocytes within the uterine stroma. Consequently, it is likely that activation of the maternal mucosal immune system, within the uterus at the same time when placentomes are being established, interferes with the process of placentome development and leads to immune-mediated abortion. Our data suggest that bovine MHC-compatible pregnancies provide a unique model for studying regulation of the uterine immune system, as well as immune-mediated placental rejection.
Asunto(s)
Enfermedades de los Bovinos/inmunología , Bovinos/inmunología , Antígenos de Histocompatibilidad Clase I/análisis , Retención de la Placenta/veterinaria , Placenta/inmunología , Complicaciones del Embarazo/veterinaria , Aborto Veterinario/inmunología , Animales , Femenino , Histocompatibilidad , Retención de la Placenta/inmunología , Embarazo , Complicaciones del Embarazo/inmunología , Trofoblastos/inmunologíaRESUMEN
Despite National Institutes of Health recommendations to administer antenatal steroids as a single course to women threatening preterm delivery, repeated treatments are often given. We investigated effects of repeated dexamethasone (DM) administered to the ewe on small maternal and foetal placental arteries. We hypothesized that DM would increase responsiveness to endothelin-1 (ET-1) and norepinephrine (NE) and that foetal arteries would react differently to ET-1 and NE compared to maternal arteries. Ewes received three treatments beginning at 103, 110, and 117 days of gestation (dGA). Each treatment consisted of four IM injections of 2mg DM or saline at 12-h intervals. At 119 dGA, in vitro functional studies were performed using Mulvany wire myography and endothelin receptor (ETR) expression was quantified using real-time RTPCR and receptor ligand autoradiography. Foetal placental arteries demonstrated greater maximal contractility to ET-1 and lesser maximal contractility to NE compared to maternal arteries. DM increased the maximal contraction elicited by ET-1 and NE in foetal but not maternal placental arteries. DM also increased the abundance of type-A ETR but not type-B ETR mRNA in foetal but not maternal placental arteries. However, within the whole placentome, DM increased the abundance of type-B ETR and decreased type-A ETR mRNA, which was confirmed by similar changes in ETR binding specifically within the labyrinth region. In summary, repeated DM treatment results in agonist and vascular bed specific responses within the placenta.
Asunto(s)
Arterias/efectos de los fármacos , Dexametasona/farmacología , Endotelina-1/metabolismo , Placenta/irrigación sanguínea , Receptor de Endotelina A/metabolismo , Ovinos , Animales , Arterias/metabolismo , Decidua/irrigación sanguínea , Decidua/efectos de los fármacos , Endotelina-1/genética , Endotelina-1/farmacología , Femenino , Edad Gestacional , Norepinefrina/farmacología , Circulación Placentaria/efectos de los fármacos , Circulación Placentaria/fisiología , Embarazo , ARN Mensajero/metabolismo , Receptor de Endotelina A/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Trofoblastos/efectos de los fármacos , Vasoconstricción/efectos de los fármacos , Vasoconstricción/fisiologíaRESUMEN
A spontaneous mammary gland ductal carcinoma in situ was diagnosed in a 6-8-year-old female rhesus macaque (Macaca mulatta). To our knowledge, this is only the tenth case of spontaneous mammary gland tumors to be reported in rhesus monkeys. Despite the paucity of case reports, several theories exist to explain the occurrence of mammary tumors. The Mason Pfizer monkey virus, a type D retrovirus similar to the virus that causes simian acquired immunodeficiency syndrome, has been implicated as a possible etiologic agent. Because this virus has been isolated from normal primate mammary tissue, it is unlikely to be the sole etiologic agent. Other theories include the tumorogenic effects that androgens, growth hormones, irradiation, and aging have on the mammary gland.
Asunto(s)
Carcinoma Intraductal no Infiltrante/veterinaria , Macaca mulatta , Neoplasias Mamarias Animales/patología , Animales , Animales de Laboratorio , Carcinoma Intraductal no Infiltrante/patología , Carcinoma Intraductal no Infiltrante/cirugía , Femenino , Neoplasias Mamarias Animales/cirugía , Mastectomía Radical/veterinariaRESUMEN
The aim of this study was to identify and characterize populations of trophoblast cells in canine placenta during different stages of fetal development using lectin histochemistry. Dogs have endotheliochorial placentation and trophoblast cell invasion continues after chorioallantois villous penetration early in pregnancy, leading to formation of a labyrinth. Specialized subpopulations of cells differentiate, such as syncytial trophoblast that invades the maternal epithelium early in placentation and surrounds and forms intimate cuffs around maternal blood vessels. Marginal haematomata, which are lined by specialized phagocytic cytotrophoblast cells, form by mid-gestation. Invasive 'extravillous' cells advance into and remodel maternal endometrial tissues further. Placentas and attached uterine tissues were collected and sampled from six bitches at mid-gestation (days 31-33 of gestation) and 12 females in late gestation (day 42-term) for characterization of these tissues and identification of other populations of trophoblast cells. Uterine tissues from nonpregnant bitches were also collected at oestrus (n = 2) and during the luteal phase (n = 1). In histochemical studies, two of six biotinylated lectins that were tested stained cytotrophoblast and syncytial trophoblast cell populations differentially. Arachis hypogaea agglutinin (PNA) was specific for cytotrophoblasts in placental tissue lining villi and cytotrophoblastic cells with phagocytic or absorptive phenotypes in the necrotic zone at mid-gestation. In late gestation, cytotrophoblast cells with an absorptive phenotype at the interface between the labyrinth and lacunar glandular chambers were stained with PNA. Staining of other cells was minimal, with the exception of deep endometrial glands. Lectin binding using Maclura pomifera agglutinin (MPL) specifically stained the same cells as PNA and the population of invasive syncytial trophoblast cells remodelling maternal blood vessels and small maternal vessels at the materno-fetal interface, as well as trophoblast cells within necrotic zones at mid-gestation. Both lectins were positive for phagocytic cytotrophoblast cells lining the haematophagus organs. The results of this study demonstrate that lectin histochemistry is a useful tool for staining subpopulations of cytotrophoblast and syncytial trophoblast cells.
Asunto(s)
Perros/fisiología , Desarrollo Embrionario y Fetal/fisiología , Placenta/citología , Preñez/fisiología , Trofoblastos/citología , Animales , Perros/embriología , Femenino , Histocitoquímica , Lectinas , EmbarazoRESUMEN
This paper reviews bovine placental development, anatomy (microscopic and gross), nomenclature and classification. The paper focuses on the biology of those specialized cells that arise from the outermost layer of very early embryos, the trophoblast cells, and on placental macrophages, cells that play a key role in fetal/placental defense. Data is presented from an immunohistochemical quantitative study that characterizes the ontogeny of placental macrophages using placental tissues from 21 cows (sampled from 4 months of pregnancy through the post partum period). Understanding of bovine placental development is essential for veterinarians, pathologists, diagnosticians and researchers. Lesions of diagnostic significance can be recognized for many economically important infectious abortifacient diseases, and there is growing evidence that pregnancy failure of cloned calves is due in part to unexplained placental failure. Placentology and placental pathology are becoming of increasing importance.
Asunto(s)
Bovinos/embriología , Trabajo de Parto/fisiología , Placentación , Animales , Membranas Extraembrionarias/fisiología , Femenino , EmbarazoRESUMEN
In most mammals trophoblast cells do not express major histocompatibility complex (MHC) antigens. This probably protects the placenta from immune attack. We have used immunohistochemistry to study the ontogeny of MHC class I expression by bovine trophoblast and endometrial epithelial cells. The interplacentomal, placentomal arcade and placentomal villous/crypt regions were studied. In the interplacentomal region a substantial proportion of trophoblast cells were class I positive from the sixth month on and about half of the endometrial epithelium was class I positive throughout pregnancy. In the arcade region trophoblast class I expression was first observed in the sixth month, increased slowly and peaked at term. Here there was no endometrial epithelial class I expression until term and then only a small percentage of cells were positive. In contrast, in the placentomal villous/crypt region both trophoblast and endometrial epithelium were class I negative throughout gestation. This study shows that cattle have extensive trophoblast class I expression. Moreover class I expression on placentomal, cryptal endometrial epithelium is shut down. Because binucleate trophoblast cells migrate and fuse with endometrial epithelial cells, total shut down of class I expression in areas of intimate interdigitation may be critical for avoidance of immunological rejection.
Asunto(s)
Antígenos de Histocompatibilidad Clase I/metabolismo , Placenta/inmunología , Útero/inmunología , Animales , Bovinos , Endometrio/metabolismo , Células Epiteliales/inmunología , Femenino , Inmunohistoquímica , Intercambio Materno-Fetal/inmunología , Embarazo , Trofoblastos/inmunologíaRESUMEN
An aged Saanen doe was euthanized following repeated severe hemorrhage from the vulva. Necropsy examination revealed mural thickening of tubular genitalia with firm, protruding intralumenal masses containing blood-filled cavitations, and effacement of normal cervical architecture. Histologically, uterine and cervical masses comprised a variably dense population of mildly pleomorphic spindle cells forming interlacing streams supported by variably dense collagenous stroma. Immunoperoxidase staining of neoplastic cells was positive for muscle-specific actin, supporting the diagnosis of low-grade leiomyosarcoma. Months later, the doe's twin was likewise euthanized due to persistent bleeding from the vulva associated with a large vulvar mass having histopathologic features similar to those of the previous case. The clinical, gross, and histologic findings are similar to five cases of caprine genital leiomyosarcoma identified in retrospectively analyzed case material. Analysis of caprine tumor accessions over 20 years demonstrated a significantly higher incidence of genital leiomyosarcoma within the Saanen breed.
Asunto(s)
Neoplasias de los Genitales Femeninos/veterinaria , Enfermedades de las Cabras/patología , Leiomiosarcoma/veterinaria , Animales , Anticuerpos Monoclonales , Resultado Fatal , Femenino , Neoplasias de los Genitales Femeninos/patología , Genitales Femeninos/patología , Cabras , Hemorragia/veterinaria , Inmunohistoquímica , Leiomiosarcoma/patología , Estudios RetrospectivosAsunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Neoplasias Ováricas/veterinaria , Teratoma/veterinaria , Animales , Diagnóstico Diferencial , Enfermedades de los Perros/patología , Perros , Femenino , Neoplasias Ováricas/diagnóstico por imagen , Neoplasias Ováricas/patología , Radiografía , Teratoma/diagnóstico por imagen , Teratoma/patologíaRESUMEN
We report the results of investigating a pregnant baboon that developed hypertension, proteinuria, and oedema in late gestation. Although the clinical presentation suggested a diagnosis of pre-eclampsia, the evolution of her clinical signs and results of a renal biopsy performed 3 weeks after delivery suggested that glomerulonephritis was the underlying disease.
Asunto(s)
Enfermedades de los Monos/patología , Papio , Preeclampsia/veterinaria , Anciano , Animales , Presión Sanguínea/fisiología , Femenino , Humanos , Riñón/patología , Enfermedades de los Monos/sangre , Nefritis Intersticial/patología , Nefritis Intersticial/veterinaria , Preeclampsia/patología , EmbarazoRESUMEN
Clinical, pathological, immunohistochemical, serological and microbiological findings are described for 2 geographically and temporally distinct equine arteritis virus (EAV) epidemics in newborn foals. Outbreak A occurred at a commercial Standardbred breeding facility; Outbreak B began in a group of research animals. Clinical signs were severe and primarily referable to the respiratory tract. Fever and leucopenia and/or thrombocytopenia were observed in foals surviving for more than 24 h. The most common gross pathological findings were limited to the respiratory tract. Common histopathological findings included interstitial pneumonia, lymphocytic arteritis and periarteritis with fibrinoid necrosis of the tunica media. Renal tubular necrosis was noted in 2 foals. Immunoperoxidase histochemistry combined with virus isolation was diagnostic in all cases.
Asunto(s)
Animales Recién Nacidos , Infecciones por Arterivirus/veterinaria , Brotes de Enfermedades/veterinaria , Equartevirus/aislamiento & purificación , Enfermedades de los Caballos/patología , Animales , Infecciones por Arterivirus/complicaciones , Infecciones por Arterivirus/epidemiología , Infecciones por Arterivirus/patología , Femenino , Fiebre/veterinaria , Enfermedades de los Caballos/sangre , Enfermedades de los Caballos/virología , Caballos , Inmunohistoquímica , Túbulos Renales/patología , Túbulos Renales/virología , Leucopenia/veterinaria , Pulmón/irrigación sanguínea , Pulmón/patología , Pulmón/virología , Enfermedades Pulmonares Intersticiales/veterinaria , Masculino , Necrosis , Nefritis Intersticial/veterinaria , Trombocitopenia/veterinariaAsunto(s)
Enfermedades de los Perros/diagnóstico por imagen , Neoplasias Endometriales/veterinaria , Pólipos/veterinaria , Animales , Diagnóstico Diferencial , Enfermedades de los Perros/patología , Perros , Neoplasias Endometriales/diagnóstico por imagen , Neoplasias Endometriales/patología , Femenino , Pólipos/diagnóstico por imagen , Pólipos/patología , UltrasonografíaRESUMEN
Male Dutch rabbits were weighed and randomly assigned within each weight group to five groups of six animals each (plus one more in the highest dose group). They received 0, 12.5, 25.0, 37.5, or 50.0 mg of ethylene glycol monomethyl ether (EGME) per kg of body weight in the drinking water 5 d/week for 12 weeks. Feed and water consumption were monitored daily and body weight weekly. All animals consumed the water and feed, maintained body weight, and were in good health throughout the experiment. Semen was collected twice weekly for 12 weeks, and 96% of the ejaculates were obtained. By weeks 6 and 9, most males in groups receiving 50.0 or 37.5 mg of EGME per kg were oligospermic. Only minor changes in other characteristics of sperm obtained from treated animals were found, as measured by computer-assisted sperm analysis. Fertility of the males still producing sufficient sperm during week 12 to use for insemination was tested with 96 does producing 2839 oocytes, and fertility of treated males (41%) was not lower (P > 0.05) than 47% in controls. At necropsy, all vital organs were grossly normal, with no notable histopathology. However, the groups of animals receiving 37.5 and 50 mg of EGME per kg of body weight produced fewer sperm and had smaller testes than controls (P < 0.05). Although all rabbits appeared grossly normal, there was a marked disruption of spermatogenesis as ingestion of EGME increased above 25 mg/kg of body weight. Rabbit testes appear to be more sensitive to EGME than testes of rats or mice.