Genotypic characterization of Streptococcus didelphis causative of fatal infection in white-eared opossums.

Streptococcus didelphis was once reported as related to severe infections in opossums. Thus, we present the first comprehensive whole-genome characterization of clinical S. didelphis strains isolated from white-eared opossums (Didelphis albiventris). Long-read whole-genome sequencing was performed using the MinION platform, which allowed the prediction of several genomic features. We observed that S. didelphis genomes harbor a cluster for streptolysin biosynthesis and a conserved genomic island with genes involved in transcriptional regulation (arlR) and transmembrane transport (bcrA). Antimicrobial resistance genes for several drug classes were found, including beta-lactam, which is the main antimicrobial class used in Streptococcus spp. infections; however, no phenotypical resistance was observed. In addition, we predicted the presence of 33 virulence factors in the analyzed genomes. High phylogenetic similarity was observed between clinical and reference strains, yet no clonality was suggested. We also proposed dnaN, gki, pros, and xpt as housekeeping candidates to be used in S. didelphis sequence typing. This is the first whole-genome characterization of S. didelphis, whose data provide important insights into its pathogenicity.

Hiperplasia endometrial cística em uma coelha-doméstica (Oryctolagus cuniculus domesticus) / Cystic endometrial hyperplasia in a domestic rabbit (Oryctolagus cuniculus domesticus)

Background: Cystic endometrial hyperplasia is a hormone-dependent disease induced by systemic increase in progesteronethat can occur in several domestic species, such as the rabbit. This disease may be associated with sex steroid hormones,especially progesterone, and may be asymptomatic, and it is diagnosed using complementary imaging tests such as totalabdominal ultrasound. However, surgical excisional biopsy with histopathological tissue analysis is the gold standard. Thisstudy reports a case of asymptomatic cystic endometrial hyperplasia in a female Miniature Lion Lop rabbit (Oryctolaguscuniculus domesticus) treated with therapeutic ovariohysterectomy.Case: A domestic, adult, female Miniature Lion Lop rabbit (Oryctolagus cuniculus domesticus), aged approximately 5 yearsand weighing 3.2 kg, was referred to specialized care to undergo ovariohysterectomy, an elective procedure. The tutor onlyreported the occurrence of a single episode of vulvar secretion more than 2 years ago, treated with antibiotics, with remissionof clinical signs. In the intraoperative period after celiotomy, the uterine horn and uterine body showed a significant increasein volume, with abnormal color changes and tissue consistency; however, both changes were clinically asymptomatic. Subsequently, biopsy was performed during the ovariohysterectomy procedure. The excised uterus and ovaries were placed in 10%formalin and histopathologically analyzed. The macroscopic histopathological examination of the sectioned tissue revealed aslight amount of brownish fluid inside the uterine horns, in addition to multiple cystic areas in the uterine mucosa. Microscopicexamination revealed marked hyperplasia of well-differentiated endometrial epithelial cells, occasionally forming cystic structuresof different sizes. Moderate congestion, mild multifocal hemorrhage, and mild multifocal inflammatory infiltrate in the lamina...

Intussuscepção intestinal associada à protrusão cloacal em Trachemys scripta elegans / Intestinal intussusception associated with cloacal protusion in Trachemys scripta elegans

Background: Gastrointestinal dysfunction in reptiles is a common condition seen in animal medicine, and is often causedby inappropriate husbandry. The purpose of this report is to describe the case of a surgical procedure for enterectomy ofthe small intestines, performed as treatment for an intussusception with cloacal protrusion that occurred in a red-earedslider (Trachemys scripta elegans) kept as a pet.Case: A 20-year-old red-eared slider (Trachemys scripta elegans) was taken into medical care after the owners observationof a cloacal protrusion that had started 72 h previously. During physical examination the protrusion was noted as an intestinalsegment of approximately 5 cm, which was not reducible. Complementary examinations, including radiography and hematological profiling, were performed and revealed no significant findings; therefore, it was decided that an exploratory celiotomywould be conducted. The patient was referred to the surgical unit for the procedure and underwent surgical anesthesia. Afterappropriate antisepsis of the surgical area, a plastron osteotomy was performed using a previously sterilized oscillatory saw ata 45º angulation. The celomatic membrane was subsequently incised to enable both cavity and intestinal inspection allowingobservation of the intussusception in the small intestine of the animal with the intussuscept segment protruding through thecloaca. The intussusception was undone, and an enterectomy was performed to remove the unviable intestine, using intestinalresection and subsequent anastomosis with simple interrupted sutures using 4-0 nylon, followed by intestinal reposition in thecavity. The celomatic membrane was closed using continuous suture with 4-0 nylon. The plastron fragment was then repositioned with the aid of eight...(AU)

Intussuscepção intestinal associada à protrusão cloacal em Trachemys scripta elegans / Intestinal intussusception associated with cloacal protusion in Trachemys scripta elegans

Background: Gastrointestinal dysfunction in reptiles is a common condition seen in animal medicine, and is often causedby inappropriate husbandry. The purpose of this report is to describe the case of a surgical procedure for enterectomy ofthe small intestines, performed as treatment for an intussusception with cloacal protrusion that occurred in a red-earedslider (Trachemys scripta elegans) kept as a pet.Case: A 20-year-old red-eared slider (Trachemys scripta elegans) was taken into medical care after the owner’s observationof a cloacal protrusion that had started 72 h previously. During physical examination the protrusion was noted as an intestinalsegment of approximately 5 cm, which was not reducible. Complementary examinations, including radiography and hematological profiling, were performed and revealed no significant findings; therefore, it was decided that an exploratory celiotomywould be conducted. The patient was referred to the surgical unit for the procedure and underwent surgical anesthesia. Afterappropriate antisepsis of the surgical area, a plastron osteotomy was performed using a previously sterilized oscillatory saw ata 45º angulation. The celomatic membrane was subsequently incised to enable both cavity and intestinal inspection allowingobservation of the intussusception in the small intestine of the animal with the intussuscept segment protruding through thecloaca. The intussusception was undone, and an enterectomy was performed to remove the unviable intestine, using intestinalresection and subsequent anastomosis with simple interrupted sutures using 4-0 nylon, followed by intestinal reposition in thecavity. The celomatic membrane was closed using continuous suture with 4-0 nylon. The plastron fragment was then repositioned with the aid of eight...

Estabilização de sínfise mandibular com cerclagem após disjunção traumática em cachorro-do-mato (Cerdocyon thous) / Mandibular symphysis stabilization after traumatic disjunction in a crab-eating fox (Cerdocyon thous)

Background: Fractures and dislocations are common orthopedic conditions arising from traumas from anthropic interaction on wild species, such as those caused by road trampling, being the mandibular separation with great frequency of occurrence in dogs and cats. The procedures for mandibular symphysis stabilization are described as of low complexity in the literature, however no reports of this procedure in wild canids were found. The purpose of this study is to report a surgical procedure of mandibular symphysis disjunction stabilization in an individual of the species Cerdocyon thous (crab-eating fox) through the technique of cerclage fixation using stainless steel wire.Case: An adult male individual of the free-living specie weighing 5.6 kg was referred to veterinary care after having been run-over. General physical examination showed abrasions in the ventral region of the jaw, discreet bleeding in the oral cavity, and mandibular instability. Subsequently, the patient underwent radiographic examination that revealed mandibular disjunction. The patient was therefore referred to the surgical unit for stabilization. During surgical anesthesia, two 12-gauge hypodermic needles (40x12 mm) were inserted percutaneously through the ventral region of the mandible, protruding into the oral cavity immediately caudal to the canine teeth. Next, both extremities of a 0.5 mm stainless steel wire were introduced into the interior of the needles and, when both ends extruded through the other end of the needles, the latter were removed, leaving only the steel wire uniting both rostral rami of the mandible. Using a twisting apparatus, the steel wire was twisted until the disjunction was completely stabilized and mandibular occlusion was deemed appropriate. Lastly, the surplus steel wire was sectioned, leaving a small segment that remained exposed to surface in the ventral region of the mandible.[...]

Estabilização de sínfise mandibular com cerclagem após disjunção traumática em cachorro-do-mato (Cerdocyon thous) / Mandibular symphysis stabilization after traumatic disjunction in a crab-eating fox (Cerdocyon thous)

Background: Fractures and dislocations are common orthopedic conditions arising from traumas from anthropic interaction on wild species, such as those caused by road trampling, being the mandibular separation with great frequency of occurrence in dogs and cats. The procedures for mandibular symphysis stabilization are described as of low complexity in the literature, however no reports of this procedure in wild canids were found. The purpose of this study is to report a surgical procedure of mandibular symphysis disjunction stabilization in an individual of the species Cerdocyon thous (crab-eating fox) through the technique of cerclage fixation using stainless steel wire.Case: An adult male individual of the free-living specie weighing 5.6 kg was referred to veterinary care after having been run-over. General physical examination showed abrasions in the ventral region of the jaw, discreet bleeding in the oral cavity, and mandibular instability. Subsequently, the patient underwent radiographic examination that revealed mandibular disjunction. The patient was therefore referred to the surgical unit for stabilization. During surgical anesthesia, two 12-gauge hypodermic needles (40x12 mm) were inserted percutaneously through the ventral region of the mandible, protruding into the oral cavity immediately caudal to the canine teeth. Next, both extremities of a 0.5 mm stainless steel wire were introduced into the interior of the needles and, when both ends extruded through the other end of the needles, the latter were removed, leaving only the steel wire uniting both rostral rami of the mandible. Using a twisting apparatus, the steel wire was twisted until the disjunction was completely stabilized and mandibular occlusion was deemed appropriate. Lastly, the surplus steel wire was sectioned, leaving a small segment that remained exposed to surface in the ventral region of the mandible.[...](AU)

Development and cross-species testing of western bluebird (Sialia mexicana) microsatellite primers.

Western and eastern bluebirds (Sialia mexicana and S. sialis) are socially monogamous passerines that engage in extra-pair copulations. We obtained microsatellites from S. mexicana and optimized and characterized 15 microsatellite DNA loci in 60 individuals of this species. Primer pairs yielded an average of 13 alleles per locus in western bluebirds (range 3-35 alleles) with an average observed heterozygosity of 0.68 (range 0.27-0.88). All 15 loci also successfully amplified in S. sialis (n = 24), with an average of 11.5 alleles per locus (range 4-26) and an average observed heterozygosity of 0.59 (range 0.22-0.90).