Candidate circulating microRNA biomarkers in dogs with chronic pancreatitis.

BACKGROUND: Pancreatitis is an important cause of disease and death in dogs. Available circulating biomarkers are not sufficiently sensitive and specific for a definitive diagnosis. HYPOTHESIS: Circulating microRNAs would be differentially expressed in dogs with chronic pancreatitis and could have potential as diagnostic biomarkers. ANIMALS: Healthy controls (n = 19) and dogs with naturally occurring pancreatitis (n = 17). METHODS: A retrospective case-control study. Dogs with pancreatitis were included if they satisfied diagnostic criteria for pancreatitis as adjudicated by 3 experts. MicroRNA was extracted from stored serum samples and sequenced. Reads were mapped to mature microRNA sequences in the canine, mouse, and human genomes. Differentially expressed microRNAs were identified and the potential mechanistic relevance explored using Qiagen Ingenuity Pathway Analysis (IPA). RESULTS: Reads mapping to 196 mature microRNA sequences were detected. Eight circulating microRNAs were significantly differentially expressed in dogs with pancreatitis (≥2-fold change and false discovery rate <0.05). Four of these mapped to the canine genome (cfa-miR-221, cfa-miR-222, cfa-miR-23a, and cfa-miR-205). Three mapped to the murine genome (mmu-miR-484, mmu-miR-6240, mmu-miR-101a-3p) and 1 to the human genome (hsa-miR-1290). Expression in dogs with pancreatitis was higher for 7 microRNAs and lower for mmu-miR-101a-3p. Qiagen IPA demonstrated a number of the differently expressed microRNAs are involved in a common pancreatic inflammatory pathway. CONCLUSIONS: The significantly differentially expressed microRNAs represent promising candidates for further validation as diagnostic biomarkers for canine pancreatitis.

An undernutrition screening score for dogs with protein-losing enteropathy: A prospective multicenter study.

BACKGROUND: The impact of undernutrition in dogs with protein-losing enteropathy (PLE) caused by inflammatory enteritis, intestinal lymphangiectasia, or both and which variables are most predictive of outcome are unknown. OBJECTIVES: Develop an undernutrition screening score (USS) for use at the time of diagnosis of PLE in dogs, which is predictive of outcome. ANIMALS: Fifty-seven dogs with PLE prospectively recruited from 3 referral hospitals in the United Kingdom. METHODS: An USS based on the presence and severity of 5 variables: appetite, weight loss, and body, muscle, and coat condition and scored out of 15, with higher scores reflecting worse undernutrition, was calculated at the time of diagnosis. Follow-up information was obtained for at least 6 months. RESULTS: Dogs that failed to achieve clinical remission within 6 months had higher USS at diagnosis compared with dogs that achieved remission (median, 7.5; range, 2-14 and median, 5; range, 0-14, respectively). The USS at diagnosis gave an area under the receiver operating characteristic curve (AUC) of 0.656 for predicting nonclinical remission within 6 months, whereas a score consisting of just epaxial muscle loss and coat condition resulted in a larger AUC of 0.728. CONCLUSIONS AND CLINICAL IMPORTANCE: Of the 5 variables assessed in the USS, a combination of epaxial muscle loss and coat condition was most predictive of not achieving clinical remission within 6 months in dogs with PLE. Additional studies will help determine the effect of changes in USS and the 5 associated variables after diagnosis on outcome variables in these dogs.

Value of Probiotics in Canine and Feline Gastroenterology.

Probiotics/or synbiotics products for small animals do not fulfill the criteria required to qualify as a probiotic. Studies explaining modes of action are lacking. Outcome measures are inconsistent, with some trials assessing only nonspecific routine diagnostic parameters or fecal scores. Preliminary evidence shows that specific preparations are beneficial in parvovirus infections and acute hemorrhagic diarrhea syndrome in dogs and in Tritrichomonas fetus infection in cats. In dogs, inflammatory bowel disease specific probiotics can decrease clinical severity. More studies focusing on functional outcomes in dogs and cats with well-defined diseases to allow evidence-based clinical use of probiotics and synbiotics are needed.

Use of contrast-enhanced ultrasound for the diagnosis of idiopathic renal hematuria in a dog.

Background: Idiopathic renal hematuria (IRH) generally occurs in healthy large-breed dogs that are <2-years-old. It is characterized by recurrent bleeding from the kidneys of unidentified cause. The final diagnosis is reached through the exclusion of primary urinary system and systemic causes of hematuria along with the direct visualization by cystoscopy of hematic urine jets at the ureteral orifice. Case Description: An 8-year-old female neutered Whippet was presented for investigation of a 4-week history of chronic intermittent macroscopic hematuria. Physical examination, systolic blood pressure, extensive laboratory workup (including coagulation profile and platelet count), urine culture, thoracic and abdominal radiographs, pneumocystogram, and double-contrast cystography were all unremarkable. B-mode ultrasound showed no abnormalities apart from a moderate amount of suspended echogenic amorphous material visible within the urinary bladder lumen. In the contrast-enhanced ultrasound (CEUS) study, a large amount of echogenic ill-defined material was noted projecting into the urinary bladder lumen from the right ureterovesical junction in the fundamental mode. This material was more conspicuous and markedly contrast-enhancing in the harmonic mode. Ultrasound contrast medium has the unique property to strictly remain within the vessels without interstitial trapping or elimination by the kidneys. Indeed, the presence of the micro-bubbles into the urinary tract lumen in the CEUS study was interpreted as a direct sign of active urinary tract bleeding. A diagnosis of IRH was reached through CEUS and B-mode ultrasound along with an extensive laboratory workup and periodic follow-up of the patient. No therapy was administered and at a 1-year follow-up, the patient was alive with no current episodes of macroscopic hematuria reported. Conclusion: To the authors' knowledge, this is the first report describing the use of CEUS for the characterization of IRH. CEUS could represent a safe, non-invasive, affordable novel alternative technique to cystoscopy or cystotomy for the real-time diagnosis of IRH.