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1.
PLoS One ; 16(11): e0259414, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-34727141

RESUMEN

An experiment was conducted to evaluate the feed intake, nutrient digestibility and selected rumen parameters in feedlot bulls fed diets containing different feed additives. Six rumen-cannulated Nellore bulls (age = 8 ± 1.0 months; initial BW = 225 ± 13.2 kg) were distributed in a 6 × 6 Latin square design. Six experimental diets based on 30% corn silage and 70% concentrate on a dry matter (DM) basis were evaluated. Diets differed in feed additive on a DM basis, as follows: 1.4% bicarbonate and magnesium oxide in 3:1 ratio (BOX); 36 ppm lasalocid sodium (LAS); 30 ppm monensin sodium (MON); 25 ppm virginiamycin (VIR); 30 ppm monensin sodium plus 25 ppm virginiamycin (MV); and 3.15% commercial mineral supplement containing D-limonene and exogenous α-amylase (EOA). The experiment lasted 144 d, with six periods of 24 d. Each period consisted of 14 d for dietary adaptation, 3 d for feces and urine collection, and 7 d for omasal and ruminal digesta collection. Bulls fed the BOX diet showed greater (P < 0.05) intake of DM, organic matter (OM), neutral detergent fiber (apNDF), crude protein (CP), and starch compared to the other diets. Diets with LAS, MON, VIR, MV, or EOA did not influence (P > 0.05) the DM, OM, apNDF, CP, or starch intake of feedlot bulls. Bulls fed the EOA diet showed greater (trend; P = 0.09) ruminal digestibility of starch compared to the other diets. The feed additives did not affect (P > 0.05) the intestinal or total tract digestibility of starch, rumen pH, microbial efficiency, total rumen fluid, dilution rate, rate of intake, rate of degradation, or passage rate of the DM, OM, apNDF, and starch. In conclusion, LAS, MON, VIR, MV, and EOA diets reduced nutrient intake compared to BOX. Although all feed additives presented similar effects on rumen pH, temperature, and kinetics the presence of exogenous α-amylase in the EOA diet may increase ruminal starch digestibility and apparent total tract digestibility of DM and OM.


Asunto(s)
Ingestión de Alimentos , Rumen , Animales , Monensina , Nutrientes , Ensilaje
2.
PLoS One ; 15(8): e0237381, 2020.
Artículo en Inglés | MEDLINE | ID: mdl-32764802

RESUMEN

Two experiments were conducted: (1) to evaluate the effect of ensiling time and grain source on dietary nitrogen fractions; and (2) to verify the influence of concentrate level, processing method and grain source on intake, microbial efficiency, and digestibility by young Nellore bulls. In Experiment 1, corn and sorghum grains were milled, reconstituted to 35% moisture, and ensiled in a bag silo for 10 different times. There were three replications per ensiling time and grain source. Samples from each replication were analyzed in triplicate for total nitrogen (N), non-protein nitrogen (NPN), soluble N, insoluble N, and neutral detergent insoluble nitrogen (NDIN). In Experiment 2, five Nellore bulls were used in a 5 × 5 Latin square design. Four diets were comprised of 28.4% corn silage, 10.7% supplement, and 60.9% dry ground corn, dry ground sorghum, reconstituted and ensiled corn, or reconstituted and ensiled ground sorghum. An additional diet comprised of 45% corn silage, 10.7% supplement, and 44.3% dry ground corn (Roughage+) was used. Each experimental period lasted 22 days, with an adaptation period of 14 days followed by 5 days of total feces and urine collection and 3 days of collecting omasal samples. Data were analyzed using the MIXED procedure of SAS 9.4. The reconstitution and ensiling process reduced (P < 0.05) the insoluble N fraction, increased (P < 0.05) non-protein nitrogen of corn and sorghum grains, tended (P = 0.052) to increase microbial efficiency, and increased (P < 0.05) intestinal and total digestion of dry matter (DM), organic matter (OM), crude protein (CP), and starch. The concentrate level affected neither (P > 0.05) DM intake nor rumen pH. On the other hand, bulls fed diets based on 72% concentrate showed greater (P < 0.05) DM, OM, and CP digestibility compared with those fed a diet based on 55% concentrate. In addition, animals fed diets based on corn grains (both reconstituted and ensiled or dry) presented greater (P < 0.05) intestinal and total starch digestion compared to those fed sorghum grain. Therefore, the reconstitution process can reduce the insoluble N fraction and increase nutrient availability.


Asunto(s)
Dieta , Digestión , Nitrógeno/análisis , Sorghum/química , Zea mays/química , Animales , Bovinos , Concentración de Iones de Hidrógeno , Masculino , Rumen/química
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