Living with a tumor: A case of osteosarcoma involving the medullary region in Phrynops cf. P. geoffroanus (Testudines: Chelidae).

The individual Geoffroy's side-necked turtle, Phrynops cf. P. geoffroanus, was diagnosed postmortem with osteosarcoma associated with the forelimb through morphological and histological analysis. Osteosarcoma stands as the most prevalent primary malignant bone tumor in tetrapods. The tumor presents itself as a large mass in the distal epiphysis, characterized by spicular outgrowths and a rugose external texture. Histologically, the afflicted humerus displayed a high degree of vascularity and exhibited an extensive bone resorption process involving the medullary and endosteal regions. Notably, a clear transition between the bone marrow and cortical bone was absent, indicative of a remodeling process featuring Haversian bone system apposition. Additionally, the diaphyseal region displayed the progression of neoplastic bone tissue along the bone. For comparative purposes, we describe a humeral thin section from a healthy specimen revealing compact primary bone interrupted by cyclical growth marks which differs from the continuous growth observed in the neoplastic humerus. To assess the neoplastic bone growth rate at the mid-diaphysis level, phylogenetic eigenvector maps (PEM) were employed, utilizing osteocyte density and vascular density as explanatory variables. The findings indicated that the osteosarcoma exhibited a slow-growing nature, suggesting that the turtle had to live with this condition for years. As the neoplasia continued to expand, it likely led to disadvantages for the pathological Phrynops individual due to humeral deformity. Furthermore, malignancy was associated with angiogenesis and the invasion of the medullary region by neoplastic bone tissue, raising the likelihood of metastasis as an additional factor contributing to the individual's sickness. The presence of numerous vascular canals in the diaphyseal thin section suggested a low-grade central osteosarcoma. It is worth noting that osseous neoplasms are rarely documented in Testudines, making this case of osteosarcoma in a South American freshwater chelid specimen a unique and rare occurrence.

Osteohistological characterization of notosuchian osteoderms: Evidence for an overlying thick leathery layer of skin.

Osteoderms are mineralized structures embedded in the dermis, known for nonavian archosaurs, squamates, xenarthrans, and amphibians. Herein, we compared the osteoderm histology of Brazilian Notosuchia of Cretaceous age using three neosuchians for comparative purposes. Microanatomical analyses showed that most of them present a diploe structure similar to those of other pseudosuchians, lizards, and turtles. This structure contains two cortices (the external cortex composed of an outer and an inner layers, and the basal cortex) and a core in-between them. Notosuchian osteoderms show high bone compactness (>0.85) with varying degrees of cancellous bone in the core. The neosuchian Guarinisuchus shows the lowest bone compactness with a well-developed cancellous layer. From an ontogenetic perspective, most tissues are formed through periosteal ossification, although the mineralized tissues observed in baurusuchid LPRP/USP 0634 suggest a late metaplastic development. Histology suggests that the ossification center of notosuchian osteoderm is located at the keel. Interestingly, we identified Sharpey's fibers running perpendicularly to the outer layer of the external cortex in Armadillosuchus arrudai, Itasuchus jesuinoi, and Baurusuchidae (LPRP/USP 0642). This feature indicates a tight attachment within the dermis, and it is evidence for the presence of an overlying thick leathery layer of skin over these osteoderms. These data allow a better understanding of the osteohistological structure of crocodylomorph dermal bones, and highlight their structural diversity. We suggest that the vascular canals present in some sampled osteoderms connecting the inner layer of the external cortex and the core with the external surface may increase osteoderm surface and the capacity of heat transfer in terrestrial notosuchians.

Osteohistology and microanatomy of a new specimen of Cearachelys placidoi (Testudines: Pleurodira) a side-necked turtle from the lower Cretaceous of Brazil.

Pelomedusoides had a recognised diverse Early Cretaceous fauna of turtles in Northeast Brazil. Within them, the bothremydid Cearachelys placidoi has a relatively abundant record. This diversity contrasts with the scarcity of osteohistological studies in this group. The relatively well-preserved shell of C. placidoi (UFRPE 5600), from the Aptian age of Araripe Basin, is studied here for the purpose of filling this gap. We performed the osteohistological description of the plastron of a new specimen of C. placidoi. Also, to conduct a comparative study, we carried out histological sections in bones of the recent chelid Phrynops sp. Our histological findings indicate similarities between the histological pattern of freshwater turtles and the coastal marine C. placidoi. Moreover, osteohistological features imply metaplastic incorporation of dermal interwoven structural fiber bundles. Lastly, the xiphiplastron of C. placidoi shows an increase of bone compactness over its craniocaudal axis. This microanatomical feature is exclusive to Cearachelys so far.

Os Pelomedusoides tiveram uma reconhecida biodiversidade durante o Cretáceo inferior do Nordeste brasileiro. Dentre eles, o bothremydideo, Cearachelys placidoi tem um registro relativamente abundante. Essa diversidade contrasta com a escassez de estudos osteohistológicos neste grupo. Em relativa boa preservação, o casco de C. placidoi (UFRPE 5600), de idade aptiana da Bacia do Araripe, é estudado aqui com o propósito de preencher esta lacuna. Nós realizamos a descrição osteohistológica do plastrão do novo espécime de C. placidoi. Ademais, para conduzir o estudo comparativo, nós realizamos secções histológicas em ossos do quelídeo recente, Phrynops sp. Nossas descobertas histológicas indicam similaridades entre o padrão histológico das tartarugas de água doce e da marinha costeira C. placidoi. Além disso, as características osteohistológicas implicam na incorporação metaplástica de feixes de fibras dérmicas entrelaçadas. Finalmente, o xifiplastrão de C. placidoi mostra um aumento da compactação óssea ao longo do eixo craniocaudal. Esta característica microanatômica é exclusiva de Cearachelys até o momento.