Development of a highly sensitive glucose nanocomposite biosensor based on recombinant enzyme from corn.

BACKGROUND: Enzymes are biocatalysts that play a vital role in the production of biomolecules. Plants can be a valuable and cost-effective source for producing well-structured recombinant enzymes. Glucose is one of the most important biological molecules, providing energy to most living systems. An electrochemical method for immobilization of enzyme is promising because it is economic, generates less component waste, improves the signal-to-noise ratio, leads to a lower limit of detection, and stabilizes and protects the enzyme structure. RESULTS: A glucose biosensor was constructed using polyaniline (PANI) and a recombinant enzyme from corn, plant-produced manganese peroxidase (PPMP), with polymerization of aniline as a monomer in the presence of gold nanoparticles (AuNPs)-glucose oxidase (GOx), and bovine serum albumin. Using linear sweep voltammetry and cyclic voltammetry techniques, PANI-AuNPs-GOx-PPMP/Au electrode exhibited a superior sensing property with a wider linear range of 0.005-16.0 mm, and a lower detection limit of 0.001 mm compared to PANI-GOx-PPMP/Au electrode and PANI-GOx-PPMP/AuNPs/Au electrode. The biosensor selectivity was assessed by determining glucose concentrations in the presence of ascorbic acid, dopamine, aspartame, and caffeine. CONCLUSION: We conclude that a plant-produced Mn peroxidase enzyme combined with conductive polymers and AuNPs results in a promising nanocomposite biosensor for detecting glucose. The use of such devices for quality control in the food industry can have a significant economic impact. © 2022 Society of Chemical Industry.

Narrow pH response multilayer films with controlled release of ibuprofen on magnesium alloy.

An intelligent narrow pH-triggered multilayer film was prepared on magnesium alloys, aiming to solve the implant infections during the implantation period and improve the corrosion resistance of magnesium alloys. The encapsulation of ibuprofen by chitosan (IBU@CS) makes the release of IBU sensitive to narrow pH (pH 6.8-7.4). Positive charged IBU@CS was assembled with heparin (Hep) to fabricate (Hep/IBU@CS)10 film on AZ31 alloys using layer-by-layer method. The microstructure, composition and anticorrosion properties of the film were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and electrochemical experiments. Cellular activity was studied by MTT cell viability assay. The results showed that the Hep/IBU@CS multilayer films improved the corrosion resistance of magnesium alloys. The in vitro test demonstrated that the release of IBU in the film presented narrow pH sensitivity. The films showed no obvious signs of cytotoxicity conformed by the MTT assay and presented antibacterial properties. These preliminary results demonstrate the potential use of the Hep/IBU@CS multilayer films on magnesium-based implants.

Magnetic nanocellulose-magnetite aerogel for easy oil adsorption.

HYPOTHESIS: Cellulose aerogels are a new category of high-efficiency adsorbents for treating oil spills and water pollution. However, the hydrophilic properties and recyclability of aerogels after adsorption hamper developments and applications. Combining both hydrophobic and magnetic properties are expected to improve their adsorption capacity and functionality. EXPERIMENTS: In this study, the effect of oleic acid (OA) and nanomagnetite on the preparation of magnetic nanocellulose aerogels (called as NCA/OA/Fe3O4) by a mechanical mixing combined with freeze-drying method have been investigated. FINDINGS: It has been found that the optimal condition for fabricating this NCA/OA/Fe3O4 aerogel is 0.4 wt% nanocellulose, 3 mg mL-1 OA and 0.5 wt% Fe3O4 in the aqueous solution. This aerogel has a very low density of 9.2 mg cm-3 and demonstrates a high adsorption capacity of 68.06 g g-1 for cyclohexane. In addition, this aerogel adsorbent demonstrates an excellent magnetic responsivity and can be easily recycled by a permanent magnet after adsorption. As a consequence, this hydrophobic magnetic NCA/OA/Fe3O4 aerogel is promising not only for easy oil and organic solvent adsorption but also potentially for other magnetic related applications.