Asunto(s)
Lesión Renal Aguda/diagnóstico , Amanita/patogenicidad , Lesión Renal Aguda/etiología , Lesión Renal Aguda/terapia , Anciano , Creatinina/sangre , Soluciones Cristaloides/uso terapéutico , Femenino , Fluidoterapia , Humanos , Riñón/diagnóstico por imagen , Riñón/patología , Masculino , Persona de Mediana Edad , Intoxicación por Setas/complicaciones , Intoxicación por Setas/diagnóstico , UltrasonografíaRESUMEN
A yellow, aerobic, Gram-stain-negative, non-motile, rod-shaped and non-flagellated bacterial strain, designated T16R-129T, was isolated from the rhizosphere of a tomato plant collected at a farm located on Buyeo-gun of Chungcheongnam-do, South Korea. Strain T16R-129T grew at 15-40 °C and pH 7.0-9.0, and did not require NaCl for growth. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T16R-129T clustered with members of the genus Terrimonas, and it shared highest similarity with Terrimonas arctica R9-86T (96.1â%), Terrimonas pekingensis QHT (95.9â%), Terrimonas lutea DYT (94.9â%), Terrimonas crocea M1-33108T (95.4â%) and Terrimonas rhizosphaerae CR94T (95.3â%). The major isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C15â:â0, iso-C17â:â0 3-OH and iso-C15â:â1 G. The polar lipids of strain T16R-129T were phosphatidylethanolamine, two unidentified aminolipids, two unidentified aminophospholipids and five unidentified polar lipids. The G+C content of the genomic DNA was 46.0 mol%. On the basis of data from this polyphasic taxonomic study, strain T16R-129T represents a novel species in the genus Terrimonas, for which the name Terrimonas terrae sp. nov. is proposed; the type strain is T16R-129T (=KACC 18787T=JCM 31603T).
Asunto(s)
Bacteroidetes/clasificación , Filogenia , Rizosfera , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
An aerobic, Gram-stain-negative, non-spore-forming, non-flagellated, rod-shaped or filamentous bacterial strain, T16R-86T, was isolated from rhizosphere of a tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. It grew at the temperature range 10-37 °C (optimum, 28 °C) and pH range 6.0-9.0 (optimum, pH 7.0), and tolerated up to 2â% (w/v) NaCl. According to 16S rRNA gene sequence analysis, strain T16R-86T shared the highest similarity with Chitinophaga barathri YLT18T (96.8â%) and C. pinensis DSM 2588T (96.7â%), forming a subcluster with C. barathri YLT18T, C. cymbidii R156-2T and C. niabensis JS13-10T in the phylogenetic tree. The major fatty acids were iso-C15â:â0, C16â:â1ω5c and iso-C17â:â0 3-OH. The predominant respiratory quinone was menaquinone MK-7. Polar lipids were phosphatidylethanolamine, five unknown aminolipids, an unknown aminophospholipid, one unknown phospholipid and two unknown lipids. The DNA G+C content was 53.6 mol%. The phenotypic, chemotaxonomic and phylogenetic data showed that strain T16R-86T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga rhizosphaerae sp. nov. is proposed. The type strain is T16R-86T (=KACC 18790T=JCM 31600T).
Asunto(s)
Bacteroidetes/clasificación , Filogenia , Rizosfera , Microbiología del Suelo , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
A bacterial strain, designated T16E-198T, was isolated from the rhizosphere of tomato plant collected from a farm on Buyeo-gun, Chungcheongnam-do, South Korea. The strain was aerobic, Gram-stain-negative, rod-shaped, non-flagellated and yellow-pigmented. Strain T16E-198T was mesophilic, catalase- and oxidase-positive and with flexirubin-type pigments. A phylogenetic tree based on 16S rRNA gene sequences showed that strain T16E-198T formed a lineage with Parafilimonas terrae 5GHs7-2T, sharing highest sequence similarity of 98.4â% with it and less than 93â% with all the other validly published species. The major fatty acids were iso-C15â:â0, iso-C17â:â0 3-OH and iso-C15â:â1 G. The predominant menaquinone was MK-7. The polar lipids were phosphatidylethanolamine, one unknown aminophospholipid, five unknown aminolipids and five unknown lipids. The DNA G+C content was 41.2 mol%. On the basis of the phenotypic, phylogenetic and chemotaxonomic data presented, strain T16E-198T is considered to represent a novel species of the genus Parafilimonas, for which the name Parafilimonas rhizosphaerae sp. nov. is proposed; the type strain is T16E-198T (=KACC 18786T=JCM 31601T).
Asunto(s)
Bacteroidetes/clasificación , Filogenia , Rizosfera , Microbiología del Suelo , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
A bacterial strain, designated T20R-70T, was isolated from tomato rhizosphere soil collected in Yecheon-gun, Gyeongsangbuk-do in South Korea. Growth was observed within the ranges 10-40 °C (optimally at 28-30 °C), pH 7.0-8.0 (optimally at pH 7.0) and 0-1â% NaCl (optimally at 0â%). The 16S rRNA gene sequence showed the highest similarities with those of Lysobacter hankyongensis KTCe-2T (98.7â%), Lysobacter brunescens KCTC 12130T (98.0â%), 'Lysobacter daecheongensis' Dae08 (97.2â%) and Lysobacter oligotrophicus 107-E2T (97.1â%). The phylogenetic tree showed that strain T20R-70T formed a clade with Lysobacterhankyongensis KTCe-2T and Lysobacterbrunescens KCTC 12130T. The dominant fatty acids (>10â%) were iso-C15â:â0, iso-C16â:â0, iso-C17â:â1ω9c and summed feature 3 (including iso-C15â:â0 2-OH and/or iso-C16â:â1ω7c). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The major respiratory quinone was Q-8. DNA-DNA hybridization data revealed that strain T20R-70T had a hybridization value of 42±4â% (mean±sd) to the most closely related species of the genus Lysobacter. The DNA G+C content was 63.0 mol%. The physiological, biochemical and chemotaxonomic data allowed the discrimination of the new isolate from its phylogenetic relatives. Strain T20R-70T is thus considered to be a representative of a novel species of the genus Lysobacter, for which the name Lysobactersolanacearum sp. nov. is proposed. The type strain is T20R-70T (=KACC 18656T=NBRC 111881T).
Asunto(s)
Lysobacter/clasificación , Filogenia , Rizosfera , Microbiología del Suelo , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , ADN Bacteriano/genética , Ácidos Grasos/química , Lysobacter/genética , Lysobacter/aislamiento & purificación , Hibridación de Ácido Nucleico , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
A bacterial strain, designated T16R-265T, was isolated from rhizosphere of tomato in Buyeo-gun, Chungcheongnam-do, South Korea. The strain was Gram-stain-negative, aerobic, non-flagellated and rod- or long-rod-shaped. It grew at the range of 10-40 °C (optimum 28-30 °C), pH 6.0-9.0 (optimum pH 7.0) and with 0-3 % (w/v) NaCl (optimum 0 %). 16S rRNA gene sequence analysis showed that strain T16R-265T had the highest sequence similarity (98.0 %) with Flavitalea soli KIS20-3T and low sequence similarities of below 95 % with all other taxa. The phylogenetic tree revealed that the strain formed a robust monophyletic line with Flavitalea soli KIS20-3T separable from other clades. The predominant quinone of strain T16R-265T was MK-7, the polar lipids consisted of large amounts of phosphatidylethanolamine and an unknown lipid, and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G. DNA-DNA hybridization values with Flavitalea soli KACC 17319T were below 70 %. The DNA G+C content of strain T16R-265T is 46.2 mol%. Based on phylogenetic evidence and the results of phenotypic, genotypic and chemotaxonomic analyses, strain T16R-265T represents a novel species of a new genus, for which the name Pseudoflavitalea rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of the type species is T16R-265T (=KACC 18655T=NBRC 111880T). We also propose the reclassification of Flavitalea soli as Pseudoflavitalea soli comb. nov. (type strain KIS20-3T=KACC 17319T=JCM 19937T).
Asunto(s)
Filogenia , Rizosfera , Solanum lycopersicum/microbiología , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Hibridación de Ácido Nucleico , Fosfatidiletanolaminas/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
A rod-shaped, yellow, Gram-stain-negative, non-flagellated, aerobic bacterium, designated 5516J-17T, was isolated from an air sample collected from Jeju Island, Republic of Korea. It grew in the temperature range of 10-37 °C (optimum 28-30 °C), pH 6.0-11.0 (optimum, pH 7.0) and 0-1 % NaCl (w/v). Phylogenetic trees generated using 16S rRNA gene sequences revealed that strain 5516J-17T belongs to the genus Spirosoma, showing 96.9 % sequence similarity to the most closely related species, Spirosoma linguale DSM 74T. The cellular fatty acids comprised large amounts (>10 % of total fatty acids) of summed feature 3 (C16:1ω7c and/or C16:1ω6c) and C16:1ω5c, and moderate amounts (5-10 % of total fatty acids) of iso-C17:0 3-OH, iso-C15:0 and C16:0. The DNA G+C content was 55.7 mol % and MK-7 was the predominant isoprenoid quinone. Polar lipids were phosphatidylethanolamine, two unknown aminophospholipids, one unknown aminolipid and one unknown lipid. On the basis of this phenotypic and polyphasic taxonomy study, it is suggested that strain 5516J-17T represents a novel species within the genus Spirosoma, with the proposed name Spirosoma aerophilum. The type strain is 5516J-17T (= KACC 17323T = DSM 28388T = JCM 19950T).
Asunto(s)
Movimientos del Aire , Cytophagaceae/clasificación , Filogenia , Técnicas de Tipificación Bacteriana , Composición de Base , Cytophagaceae/genética , Cytophagaceae/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Fosfatidiletanolaminas/química , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
Strain 5GH22-11T, which was isolated from greenhouse soil in the Yangpyeong region, Gyeonggi province, Republic of Korea, was characterized to be an aerobic, Gram-stain-negative, flagellated, rod-shaped bacterium. It could grow at temperatures from 10 to 33 °C (optimum of 28-30 °C), in the pH range of 6.0-10.0 (optimum of pH 7.0) and without NaCl. 16S rRNA gene sequence analysis showed that strain 5GH18-14T showed the highest sequence similarities with Lysobacter niabensis GH34-4T (98.6 %), Lysobacter yangpyeongensis GH19-3T (98.1 %), 'Lysobacter fragariae' THG-DN8.7 (97.9 %), Lysobacter terrae THG-A13T (97.3 %), 'Lysobacter rhizosphaerae' THG-DN8.3 (97.2 %), 'Lysobacter tyrosinelyticus' THG-DN8.2 (97.2 %) and Lysobacter oryzae YC6269T (97.2 %), revealing less than 95.5 % sequence similarities with all other species with validly published names. Phylogenetic trees also indicated that strain 5GH18-14T formed a compact subcluster with L. niabensis GH34-4T, L. yangpyeongensis GH19-3T, L. terrae THG-A13T and L. oryzae YC6269T within the genus Lysobacter. The predominant quinone of strain 5GH18-14T was Q-8. The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylmonomethylethanolamine in large amounts, and moderate or small amounts of three unknown phospholipids and two unknown aminophospholipids. DNA-DNA hybridization values with closely related species were below 70 %. The DNA G+C content was 65.9âmol%. Based on the phylogenetic, physiological and chemotaxonomic data, it has been demonstrated that strain 5GH18-14T represents a novel species of the genus Lysobacter, for which the name Lysobacter terricola sp. nov. is proposed. The type strain is 5GH18-14T ( = KACC 16954T = JCM 30862T).
RESUMEN
A Gram-stain-negative, rod-shaped, non-flagellated, mesophilic, yellow-pigmented, aerobic bacterium, designated strain KIS20-3T, was isolated from a soil sample of Baengnyeong Island in Onjin county, Republic of Korea. According to 16S rRNA gene sequence analysis, the isolate showed highest similarities with Flavitalea populi HY-50RT (94.5 %), Niastella populi THYL-44T (94.2 %) and Flavitalea gansuensis JCN-23T (93.7 %). The neighbour-joining tree based on 16S rRNA gene sequences showed that strain KIS20-3T formed a subcluster with members of the genus Flavitalea, and this subcluster was closely related to members of the genera Niastella, Segetibacter and Parasegetibacter within the family Chitinophagaceae. The major fatty acids of strain KIS20-3T were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G, and the predominant isoprenoid quinone was menaquinone 7. The polar lipid profile comprised large amounts of phosphatidylethanolamine and one unknown polar lipid, and moderate or small amounts of four unknown aminophospholipids, two unknown aminolipids, three unknown lipids and one unknown phospholipid. The G+C content of the DNA of strain KIS20-3T was 55.7âmol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain KIS20-3T represents a novel species of the genus Flavitalea, for which the name Flavitalea soli sp. nov. is proposed. The type strain is KIS20-3T ( = KACC 17319T = JCM 19937T).
RESUMEN
An aerobic, Gram-stain-negative, non-flagellated, long rod or rod-shaped bacterial strain, 5GH22-11(T), was isolated from a soil sample of a greenhouse in Gongju, Republic of Korea. According to 16S rRNA gene sequence analysis, strain 5GH22-11(T) showed the highest sequence similarities with Niastella populi THYL-44(T) (97.1%), Niastella koreensis GR20-10T (95.7%) and Niastella yeongjuensis GR20-13(T) (95.6%), and < 3% sequence similarity with all other species with validly published names. The phylogenetic tree also showed strain 5GH22-11(T) formed a compact cluster with members of the genus Niastella. DNA-DNA hybridization revealed strain 5GH22-11(T) is a novel species, showing far less than 70% DNA-DNA relatedness with Niastella populi THYL-44(T). Strain 5GH22-11(T) contained iso-C15 : 0 (34.3%), iso-C17 : 0 3-OH (24.8%) and iso-C15 : 1 G (18.2%) as the major fatty acids; phosphatidylethanolamine, six unknown aminolipids, two unknown lipids and one unknown phospholipid as the polar lipids; and MK-7 as the predominant isoprenoid quinone. The DNA G+C content was 41.4 mol%. Based on these phylogenetic, physiological and chemotaxonomic data, it was demonstrated that strain 5GH22-11(T) represents a novel species of the genus Niastella, for which the name Niastella gongjuensis sp. nov. is proposed. The type strain is 5GH22-11(T) ( = KACC 17339(T) [corrected] = JCM 19941(T)).
Asunto(s)
Bacteroidetes , Técnicas de Tipificación Bacteriana , Bacteroidetes/clasificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/análisis , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico/genética , Fosfolípidos/análisis , Filogenia , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Suelo , Microbiología del SueloRESUMEN
A Gram-staining-negative, aerobic, non-flagellated, rod-shaped bacterial strain, KIS55-21(T), was isolated from a soil sample from Chuja Island, Jeju Province, Republic of Korea. Strain KIS55-21(T) grew optimally at pH 7.0, at 28-30°C and in the presence of 0% (w/v) NaCl. Neighbor-joining and maximum-likelihood trees based on the 16S rRNA gene sequences revealed that strain KIS55-21(T) fell within the family Xanthomonadaceae and was closely related to Metallibacterium scheffleri DKE(T). Strain KIS55-21(T) exhibited the highest 16S rRNA gene sequence similarity (92.6%) to that of M. scheffleri DKE(T), with similarities of less than 92.0% to those of the genera Dokdonella, Rhodanobacter, Aquimonas, and Frateuria. Strain KIS55-21(T) contained ubiquinone-8 (Q-8) as the predominant ubiquinone, iso-C17:0, summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl), anteiso-C17:0 and C16:0 as the major fatty acids, and phosphatidylethanolamine, aminophospholipid, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylmonomethylethanolamine as the main polar lipids. The DNA G+C content of strain KIS55-21(T) was 65.9 mol%. Differential phenotypic and chemotaxonomic properties and phylogenetic data of strain KIS55-21(T) demonstrated that this strain is distinguishable from closely related genera within the family Xanthomonadaceae. On the basis of the data presented, strain KIS55-21(T) is considered to represent a novel genus and species, for which the name Chujaibacter soli gen. nov., sp. nov. is proposed. The type strain is KIS55-21(T) (=KACC 16971(T) =DSM 28578(T)).
Asunto(s)
Microbiología del Suelo , Xanthomonadaceae/genética , Xanthomonadaceae/aislamiento & purificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/análisis , Genoma Bacteriano , Fosfolípidos/análisis , Filogenia , ARN Ribosómico 16S , República de Corea , Análisis de Secuencia de ADN , Xanthomonadaceae/clasificación , Xanthomonadaceae/crecimiento & desarrolloRESUMEN
A Gram-stain-negative, variably shaped, non-flagellated, yellow-pigmented, aerobic bacterium, designated strain SGM2-10(T), was isolated from a paddy soil sample from the Suwon region, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate was related most closely to Parasegetibacter luojiensis RHYL-37(T). Strain SGM2-10(T) showed highest 16S rRNA gene sequence similarity to P. luojiensis RHYL-37(T) (95.1â%), Flavitalea populi HY-50R(T) (95.0â%) and Flavitalea gansuensis JCN-23(T) (94.4â%). No other species in the family Chitinophagaceae shared more than 94.1â% 16S rRNA gene sequence similarity with strain SGM2-10(T). The major fatty acids of strain SGM2-10(T) were iso-C15â:â0, iso-C15â:â1 G and iso-C17â:â0 3-OH. The only menaquinone was MK-7. The polar lipids comprised phosphatidylethanolamine, seven unknown lipids and ten unknown aminolipids. The G+C content of the DNA of strain SGM2-10(T) was 46.7 mol%. On the basis of the results of the polyphasic characterization presented in this study, it is concluded that strain SGM2-10(T) represents a novel species of the genus Parasegetibacter, for which the name Parasegetibacter terrae sp. nov. is proposed. The type strain is SGM2-10(T) (â=âKACC 17341(T)â=âJCM 19942(T)). The description of the genus Parasegetibacter has also been emended.
Asunto(s)
Bacteroidetes/clasificación , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Bacteroidetes/genética , Bacteroidetes/aislamiento & purificación , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Fosfatidiletanolaminas/química , Pigmentación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaAsunto(s)
Antioxidantes/farmacología , Coprinus/metabolismo , Sesquiterpenos/farmacología , Antioxidantes/química , Benzotiazoles/química , Compuestos de Bifenilo/química , Hidroxianisol Butilado/química , Cromanos/química , Coprinus/química , Medios de Cultivo/química , Concentración 50 Inhibidora , Estructura Molecular , Picratos/química , Sesquiterpenos/química , Ácidos Sulfónicos/químicaRESUMEN
The fungal genus Suillus Micheli ex S. F. Gray plays important roles in the survival and growth of plant seedlings. Humans have utilized these ectomycorrhizal fungi to enhance the nutrient uptake and defense systems of plants, particularly in the reforestation of coniferous forests. The genus Suillus is easily distinguishable by its distinctive morphological features, although the morphology of the fruiting body does not facilitate reliable interspecies discrimination. On the basis of micro-morphological features and internal transcribed spacer sequence analysis, we found that 51 of 117 Korean Suillus specimens had initially been misidentified. The list of the 12 Suillus species previously recorded in Korea was re-evaluated and revised to only eight distinct species: S. americanus, S. bovinus, S. granulatus, S. grevillei, S. luteus, S. pictus, S. placidus, and S. viscidus. We provide taxonomical descriptions for six of these species from the sample specimens.
RESUMEN
Distinguishing individual Russula species can be very difficult due to extensive phenotypic plasticity and obscure morphological and anatomical discontinuities. In this study, we use the internal transcribed spacer (ITS) and 28S nuclear ribosomal large subunit (LSU) markers to identify and study the genetic diversity of species in the Russula subgenus Compacta in Korea. We focus on two morphologically similar species that are often misidentified for each other: R. nigricans and R. subnigricans. Based on molecular phylogenetic analyses, we identify three subgroups of R. nigricans, with two from Asia and one from Europe/North America. Surprisingly, we find Korean R. subnigricans are more closely related to R. eccentrica from North America than the type specimen of R. subnigricans from Japan. These molecular data, along with habitat data, reveal that Korean R. subnigricans had previously been misclassified and should now be recognized as R. eccentrica. Both ITS and LSU exhibit high interspecific and low intraspecific variation for R. eccentrica, R. nigricans, and R. subnigricans. These markers provide enough resolutional power to differentiate these species and uncover phylogeographic structure, and will be powerful tools for future ecological studies of Russula.
Asunto(s)
Agaricales/clasificación , Agaricales/genética , ADN de Hongos/genética , ADN Intergénico , ADN Ribosómico/genética , Bases de Datos de Ácidos Nucleicos , Ecosistema , Europa (Continente) , Marcadores Genéticos , Variación Genética , Japón , América del Norte , Filogenia , Filogeografía , República de Corea , Subunidades Ribosómicas GrandesRESUMEN
A bacterial strain, designated RHs22(T), was isolated from a soil sample cultivated with rice in the Suwon region of South Korea. The cells were aerobic, Gram-stain-negative, non-spore-forming, non-flagellated rods or occasionally filaments. The strain grew at 10-37 °C (optimum, 28-30 °C), at pH 5.0-10.0 (optimum, 7.0) and in the presence of 0-1â% (w/v) NaCl (optimum, 0â%). Phylogenetically, the strain was closely related to members of the genus Spirosoma, as its 16S rRNA gene sequence had similarity of 90.3-92.1â% with respect to those of members of the genus Spirosoma, showing the highest sequence similarity with Spirosoma panaciterrae DSM 21099(T). Strain RHs22(T) revealed relatively low sequence similarities of less than 90â% with all the other species with validly published names. It contained MK-7 as the predominant menaquinone and summed feature 3 (C16â:â1ω6c and/or C16â:â1ω7c), C16â:â1ω5c, iso-C15â:â0 and iso-C17â:â0 3-OH as the main fatty acids. The polar lipids of strain RHs22(T) were phosphatidylethanolamine, one unknown aminolipid, two unknown aminophospholipids, one unknown phospholipid and five unknown lipids. The DNA G+C content was 57.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study indicate that strain RHs22(T) represents a novel species of the genus Spirosoma, for which the name Spirosoma oryzae sp. nov. is proposed. The type strain is RHs22(T) (â=âKACC 17324(T)â=âDSM 28354(T)). An emended description of the genus Spirosoma is also proposed.
Asunto(s)
Cytophagaceae/clasificación , Oryza/microbiología , Filogenia , Microbiología del Suelo , Técnicas de Tipificación Bacteriana , Composición de Base , Cytophagaceae/genética , Cytophagaceae/aislamiento & purificación , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Fosfolípidos/química , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/químicaRESUMEN
A novel strain, designated 5GHs31-2(T), was isolated from a greenhouse soil sample collected from Yongin city, South Korea. Cells of strain 5GHs31-2(T) were Gram-stain-negative, rod-shaped, polar flagellated and yellow-pigmented. The isolate was aerobic, catalase-negative and oxidase-positive and grew optimally at 28-30 °C and pH 7.0. Strain 5GHs31-2(T) revealed the highest 16S rRNA gene sequence similarities with Fulvimonas soli LMG 19981(T) (97.6%), Dyella thiooxydans ATSB10(T) (97.5%) and Frateuria terrea VA24(T) (97.4%). Furthermore, the neighbour-joining tree showed that strain 5GHs31-2(T) was a representative of a member of the genus Fulvimonas. Strain 5GHs31-2(T) contained iso-C(16â:â0), summed feature 9 (iso-C(17â:â1)ω9c and/or C(16â:â0) 10-methyl), iso-C(15â:â0) and iso-C(17â:â0) as the major fatty acids, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an aminolipid as the main polar lipids and Q-8 as the predominant quinone. The genomic DNA G+C content of 5GHs31-2(T) was 73.0 mol%. According to DNA-DNA hybridization experiments, DNA-DNA relatedness values between strain 5GHs31-2(T) and its closest phylogenetic neighbours were below 70%. Based on the taxonomic data, strain 5GHs31-2(T) represents a novel species of the genus Fulvimonas, for which the name Fulvimonas yonginensis sp. nov. is proposed. The type strain is 5GHs31-2(T) (â=âKACC 16952(T)â=âDSM 28344(T)).
Asunto(s)
Filogenia , Microbiología del Suelo , Xanthomonadaceae/clasificación , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Vitamina K 2/análogos & derivados , Vitamina K 2/química , Xanthomonadaceae/genética , Xanthomonadaceae/aislamiento & purificaciónRESUMEN
A Gram-stain-negative bacterium, strain 5410S-62T, was isolated from an air sample collected in Suwon, Republic of Korea. It was aerobic, motile, mesophilic and formed rod-shaped cells. Colonies on R2A agar were convex, circular and pale orange with entire margins. Growth occurred at pH 5-9 (optimally at pH 7) and at 10-40 °C (optimally at 28 °C). It did not grow in the presence of 1% NaCl. Comparative analyses of 16S rRNA gene sequences demonstrated that the novel strain was closely related to members of the genus Noviherbaspirillum. Strain 5410S-62T showed the highest sequence similarity (98.2%) to Glaciimonas singularis A2-57T. It also showed high 16S rRNA gene sequence similarity (98.1-95.6%) to members of the genus Noviherbaspirillum (98.1% to Noviherbaspirillum aurantiacum SUEMI08T, 97.8% to Noviherbaspirillum soli SUEMI10T and Noviherbaspirillum canariense SUEMI03T, 97.6% to Noviherbaspirillum psychrotolerans PB1T and 95.6% to Noviherbaspirillum malthae CC-AFH3T). The strain contained summed feature 3 (C16:1ω6c and/or C16:1ω7c), C16:0 and summed feature 8 (C18:1ω6c and/or C18:1ω7c) as major fatty acids, Q-8 as the only ubiquinone and large amounts of phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. Strain 5410S-62T revealed less than 70% DNA-DNA relatedness with the type strains of closely related species of the genera Noviherbaspirillum and Herbaspirillum and Glaciimonas singularis. Based on the physiological, biochemical and chemotaxonomic data obtained in this study, it is proposed that strain 5410S-62T represents a novel species, Noviherbaspirillum suwonense sp. nov., with 5410S-62T (=KACC 16657T=âNBRC 108944T) as the type strain.
Asunto(s)
Microbiología del Aire , Oxalobacteraceae/clasificación , Filogenia , Técnicas de Tipificación Bacteriana , Composición de Base , ADN Bacteriano/genética , Ácidos Grasos/química , Datos de Secuencia Molecular , Hibridación de Ácido Nucleico , Oxalobacteraceae/genética , Oxalobacteraceae/aislamiento & purificación , ARN Ribosómico 16S/genética , República de Corea , Análisis de Secuencia de ADN , Ubiquinona/químicaRESUMEN
Two bacterial strains, designated JS4-4(T) and SHS5-24(T), were isolated from forest soil of Jeju Island and fresh water of Seoho lake in Suwon city, respectively, South Korea. Both strains were Gram-stain-negative, aerobic, motile rods. Strains JS4-4(T) and SHS5-24(T) showed high sequence similarities (97.6-95.8â%) and (96.5-95.6â%), respectively, to the members of the genus Undibacterium. The sequence similarity between strains JS4-4(T) and SHS5-24(T) was 97.0â%. A phylogenetic tree showed that these strains fell within the radius of the genus Undibacterium. The main fatty acids of strains JS4-4(T) and SHS5-24(T) were summed feature 3 (C16â:â1ω7c and/or C16â:â1ω6c) (50.1 and 58.7â%, respectively) and C16â:â0 (28.3 and 24.5â%, respectively). Both strains had ubiquinone 8 as the only respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids. Strain JS4-4(T) showed <70â% DNA-DNA hybridization with members of the genus Undibacterium. Thus, based on the evidence of a polyphasic study, it is proposed that strains JS4-4(T) and SHS5-24(T) represent two novel species, for which the names Undibacterium jejuense sp. nov. (type strain JS4-4(T)â=âKACC 12607(T)â=âNBRC 108922(T)) and Undibacterium seohonense sp. nov. (type strain SHS5-24(T)â=âKACC 16656(T)â=âNBRC 108929(T)) are proposed.