Exploration of collective tactical variables in elite netball: An analysis of team and sub-group positioning behaviours.

Collective tactical behaviours are aspects of player interactions that are particularly important in netball, due to its unique restrictions on player movement (players unable to move when in possession of the ball and positional spatial restrictions). The aim of this study was to explore variables representing collective tactical behaviours in netball. A local positioning system provided player positions of one team throughout seven elite-level netball matches. The positions were analysed to provide mean, variability (standard deviation) and irregularity (normalised approximate entropy) for each attack and defence possession (470 and 423, respectively) for the team and positional subgroups (forwards, midcourts and defenders) for 10 position-related variables. Correlational analyses showed collective tactical variables could be grouped as lateral and longitudinal dispersion variables. The variables were each analysed after log transformation with a linear mixed model to compare attack and defence and to estimate standardised effects on attack and defence of possession outcome, possession duration, score difference, match time, opposition strength and season time. During attack, the team and all sub-groups adopted greater lateral dispersion between players, while on defence there was generally greater longitudinal dispersion. The team also showed increased longitudinal dispersion when home and opposition possessions ended in a score. Additionally, greater irregularity was observed in active sub-groups (forwards on attack, defenders on defence). Score difference and opposition strength had trivial-small but generally unclear effects. In conclusion, these effects show that analysis of player positions on attack and defence is a promising avenue for coaches and analysts to modify collective tactical behaviours in netball.

A Framework for Clinicians to Improve the Decision-Making Process in Return to Sport.

Return-to-sport (RTS) decisions are critical to clinical sports medicine and are often characterised by uncertainties, such as re-injury risk, time pressure induced by competition schedule and social stress from coaches, families and supporters. RTS decisions have implications not only for the health and performance of an athlete, but also the sports organisation. RTS decision-making is a complex process, which relies on evaluating multiple biopsychosocial factors, and is influenced by contextual factors. In this narrative review, we outline how RTS decision-making of clinicians could be evaluated from a decision analysis perspective. To begin with, the RTS decision could be explained as a sequence of steps, with a decision basis as the core component. We first elucidate the methodological considerations in gathering information from RTS tests. Second, we identify how decision-making frameworks have evolved and adapt decision-making theories to the RTS context. Third, we discuss the preferences and perspectives of the athlete, performance coach and manager. We conclude by proposing a framework for clinicians to improve the quality of RTS decisions and make recommendations for daily practice and research.

Characteristics of Complex Systems in Sports Injury Rehabilitation: Examples and Implications for Practice.

Complex systems are open systems consisting of many components that can interact among themselves and the environment. New forms of behaviours and patterns often emerge as a result. There is a growing recognition that most sporting environments are complex adaptive systems. This acknowledgement extends to sports injury and is reflected in the individual responses of athletes to both injury and rehabilitation protocols. Consequently, practitioners involved in return to sport decision making (RTS) are encouraged to view return to sport decisions through the complex systems lens to improve decision-making in rehabilitation. It is important to clarify the characteristics of this theoretical framework and provide concrete examples to which practitioners can easily relate. This review builds on previous literature by providing an overview of the hallmark features of complex systems and their relevance to RTS research and daily practice. An example of how characteristics of complex systems are exhibited is provided through a case of anterior cruciate ligament injury rehabilitation. Alternative forms of scientific inquiry, such as the use of computational and simulation-based techniques, are also discussed-to move the complex systems approach from the theoretical to the practical level.

Positional Differences in the Most Demanding Scenarios of External Load Variables in Elite Futsal Matches.

The aims of this study were to analyze the peak physical demands in elite futsal by quantifying the most demanding scenarios of match play and to identify the differences between playing positions (defenders, wingers, and pivots) and the seasonal trend for five different rolling average time windows (30, 60, 120, 180, and 300 s). The most demanding scenarios of external load from distance, speed, acceleration, and deceleration variables were obtained from 14 elite futsal players using a local positioning system during 15 official matches in the premier Spanish Futsal League (2018-2019 season). The results showed an extremely large effect of the time window for all dependent variables in all positional groups. Another important finding of this study was that, in regard to the seasonal trend, only defenders reported clear moderate-large positive trends for high-speed running (>18 km⋅h-1) efforts, high-acceleration efforts, and high-deceleration efforts. Finally, moderate-large individual differences in player means for all dependent variables and clear differences between games for most dependent variables were found, suggesting how likely contextual factors may exert an influence on how "demanding" the most demanding scenarios are. The findings of this study provide coaches and strength and conditioning coaches further knowledge of the peak physical demands in elite futsal competition. This valuable information may lead to a more precise position-specific training prescription.

Convergent Validity of CR100-Based Session Ratings of Perceived Exertion in Elite Youth Football Players of Different Ages.

PURPOSE: To assess the convergent validity of internal load measured with the CR100 scale in youth football players of 3 age groups. METHODS: A total of 59 players, age 12-17 years, from the youth academy of a professional football club were involved in this study. Convergent validity was examined by calculating the correlation between session ratings of perceived exertion (sRPE) and Edwards load, a commonly used load index derived from the heart rate, with the data originating from 1 competitive season. The magnitude of the relationship between sRPE and Edwards load was obtained with weighted mean correlations and by assessing the effect of the change of the Edwards load on sRPE. Differences between the individuals' intercepts and slopes were assessed by interpreting the SD representing the random effects (player identity and the interaction of player identity and scaled Edwards load). Probabilistic decisions about true (infinite sample) magnitudes accounting for sampling uncertainty were based on 1-sided hypothesis tests of substantial magnitudes, followed by reference Bayesian analysis. RESULTS: Very high relationships exist between the sRPE and Edwards load across all age groups, with no meaningful differences in the magnitudes of the relationships between groups. Moderate to large differences between training sessions and games were found in the slopes of the relationships between the sRPE and Edwards load in all age groups. Finally, mostly small to moderate differences were observed between individuals for the intercepts and slopes of the relationships between the sRPE and Edwards load. CONCLUSION: Practitioners working in youth team sports can safely use the CR100 scale to track internal load.

Moving Toward a More Comprehensive Analysis of Acceleration Profiles in Elite Youth Football.

In football, having greater acceleration ability may decide the most important moments within matches. Up to now, commonly used acceleration variables have typically been investigated in isolation, with each variable suffering from unique limitations. Subsequently, any findings may provide a limited representation of what specific acceleration demands had actually occurred. Without gaining a comprehensive understanding of acceleration demands in football, it appears difficult to identify how to best monitor and maximize the long-term development of acceleration ability in footballers, all whilst doing so in a safe, sport-specific manner. Moving toward a more comprehensive analysis of acceleration profiles addresses this, as it can provide a more robust, informative understanding of the unique acceleration demands of competitive match-play. This perspective article aims to discuss the benefits of adopting a more comprehensive analysis of the acceleration demands during competitive matches for football players, by simultaneously analyzing high-intensity accelerations, repeated high acceleration ability (RHAA), and average acceleration. We discuss examples of the calculation and application of a more comprehensive acceleration profile at a team level throughout the course of an entire elite youth football season, as well as on an individual level. Monitoring acceleration profiles more comprehensively not only appears important from a training load/injury prevention perspective, but also, equips coaches and conditioning staff with the specific information necessary to develop and prescribe individualized, acceleration-emphasized training protocols that are replicable to the demands of match-play. Examples of such protocols are provided.

Criterion Validity of Catapult ClearSky T6 Local Positioning System for Measuring Inter-Unit Distance.

The validity of a local positioning system (LPS) to measure inter-unit distance was investigated during a team sport movement circuit. Eight recreationally active, female indoor team-sport players completed a circuit, comprising seven types of movements (walk, jog, jump, sprint, 45° change of direction and shuffle), on an indoor court. Participants wore a receiver tag (ClearSky T6, Catapult Sports) and seven reflective markers, to allow for a comparison with the reference system (©Vicon Motion Systems, Oxford Metrics, UK). Inter-unit distance was collected for each combination of participants. Validity was assessed via root mean square error, mean bias and percentage of variance accounted for, both as an overall dataset and split into distance bands. The results presented a mean root mean square error of 0.20 ± 0.05 m, and mean bias detected an overestimation for all distance bands. The LPS shows acceptable accuracy for measuring inter-unit distance, opening up opportunities to utilise player tracking for tactical variables indoors.

Validity of Session Rating of Perceived Exertion Assessed via the CR100 Scale to Track Internal Load in Elite Youth Football Players.

PURPOSE: To assess the convergent validity of the Borg CR100® scale to track internal training load (TL) in youth football players. METHODS: A total of 19 youth football players (age = 15 [1] y, height = 175.9 [12.3] cm, and body mass = 69 [15.4] kg) were monitored for 27 sessions, including training and games. Internal TL was assessed via session rating of perceived exertion (sRPE) and 2 heart-rate-based methods (Banister training impulse and Edwards TL). The correlations between sRPE and heart-rate-based TL, the differences in individual player intercepts and slopes, and the differences between types of sessions (training vs games) were assessed using a general linear mixed model with magnitude-based inferences. RESULTS: Correlations between sRPE and Banister training impulse were very large at overall group level (r = .77; 90% confidence limits, .72-.80) and individual level (range .70-.95). Correlations between sRPE and Edwards TL were very large at overall group level (r = .84; 90% confidence limit, .82-.86) and large to very large at individual level (range .64-.93). A very likely small difference was found in the comparison between games and training sessions for the relationship between sRPE and Banister training impulse. CONCLUSIONS: The Borg CR100 scale is a valid method for monitoring TL in youth football players.

Interunit Reliability and Effect of Data-Processing Methods of Global Positioning Systems.

PURPOSE: To establish the interunit reliability of a range of global positioning system (GPS)-derived movement indicators, to determine the variation between manufacturers, and to investigate the difference between software-derived and raw data. METHODS: A range of movement variables were obtained from 27 GPS units from 3 manufacturers (GPSports EVO, 10 Hz, n = 10; STATSports Apex, 10 Hz, n = 10; and Catapult S5, 10 Hz, n = 7) that measured the same team-sport simulation session while positioned on a sled. The interunit reliability was determined using the coefficient of variation (%) and 90% confidence limits, whereas between-manufacturers comparisons and comparisons of software versus raw processed data were established using standardized effect sizes and 90% confidence limits. RESULTS: The interunit reliability for both software and raw processed data ranged from good to poor (coefficient of variation = 0.2%; ±1.5% to 78.2%; ±1.5%), with distance, speed, and maximal speed exhibiting the best reliability. There were substantial differences between manufacturers, particularly for threshold-based acceleration and deceleration variables (effect sizes; ±90% confidence limits: -2.0; ±0.1 to 1.9; ±0.1), and there were substantial differences between data-processing methods for a range of movement indicators. CONCLUSIONS: The interunit reliability of most movement indicators was deemed as good regardless of processing method, suggesting that practitioners can have confidence within systems. Standardized data-processing methods are recommended, due to the large differences between data outputs from various manufacturer-derived software.

Match analysis in Rugby Union: performance indicators of Rugby Championship and Super Rugby teams.

BACKGROUND: A number of key performance indicators (KPIs) have been found to discriminate between winning and losing performances in Rugby Union competitions. This study aimed to investigate the KPIs of winning and losing teams during recent Rugby Championship and Super Rugby seasons. METHODS: Data from 139 matches during the 2014-2015 Rugby Championship and 2015 Super Rugby were extracted from the SANZAAR website and retrospectively analyzed. Seventeen KPIs were used to describe the differences between winning and losing performances in different aspects of the game. Magnitude-based inferences, effect size with 90% confidence interval, percentage changes, and statistical significance were calculated. RESULTS: Winning teams during the Rugby Championship were most likely to score more tries, and very likely to gain more meters, beat more defenders, and miss less tackles compared to losing teams. In Super Rugby, winning teams were most likely to score more tries, execute more clean breaks, kicks in play and very likely to win more turnovers and concede a lower number of turnovers. The number of tackles made, and meters gained were also likely higher in Super Rugby winning teams compared to the losing counterparts. CONCLUSIONS: A limited number of KPIs differentiated were observed between winning and losing performances in the Rugby Championship, whereas numerous attacking and defensive KPIs were found to discriminate between winning and losing teams during Super Rugby. The method of data extraction, analysis and interpretation used herein could assist with game strategies and in turn better prepare teams and players for competition.

Running Intensities in Elite Youth Soccer by Age and Position.

Duthie, GM, Thornton, HR, Delaney, JA, Connolly, DR, and Serpiello, FR. Running intensities in elite youth soccer by age and position. J Strength Cond Res 32(10): 2918-2924, 2018-The purpose of this investigation was to examine differences between the peak running speed, acceleration, and metabolic power of elite youth soccer across a range of age levels by position. Ninety-six elite junior soccer players were assessed between 2015 and 2017. Ninety-six elite junior soccer players (at time of match: age, 15.8 ± 0.9 years; body mass, 69.1 ± 8.0 kg) were assessed during 61 games within the 2015, 2016, and 2017 season, for a total of 441 individual match observations (4.8 ± 3.3 matches per player, range 1-13). Participants were classified by age group: under 15 (U15, n = 121, 14.7 ± 0.3 years), under 16 (U16, n = 176, 15.8 ± 0.3 years), or under 17 (U17, n = 144, 16.7 ± 0.4 years), and according to their playing position: Attacker (ATT), Defender (DEF), Mid-Fielder (MID), or Wide (WIDE). Participants wore global positioning system units during each match, where speed (m·min), acceleration/deceleration (m·s), and metabolic power (Pmet) were established. A 1- to 10-minute moving average was applied to establish the intercept (c) and slope (n) of running intensity variables as a power law y = cx relationship. Linear mixed models were used to examine differences in the intercept and slope between age group and player position. There were no substantial differences in peak (intercept) or decline (slope) in running intensity between playing levels. Several differences were observed in the peak running speeds (m·min), particularly peak running speeds of ATT and DEF being substantially lower than the MID. Despite variability between positions, we suggest that the magnitude of these differences would not warrant the prescription of different running intensities across positions at the elite junior level. These findings describe the peak running intensities of elite junior soccer, useful in the monitoring and prescription of training to ensure that players are prepared for the most demanding periods of competition.

The Occurrence of Repeated High Acceleration Ability (RHAA) in Elite Youth Football.

The aim of the present study was to investigate the occurrence of Repeated High-Acceleration Ability (RHAA) bouts in elite youth football games using 10-Hz GPS devices and two relative thresholds derived from players' actual maximal acceleration. Thirty-six outfield soccer players (age 14.9±0.6 years) participated in the study. Players wore 10-Hz GPS units during 41 official games. High accelerations were defined as efforts commencing above a threshold corresponding to 70% (T70%) or 80% (T80%) of the average 5-m acceleration obtained during a 40-m sprint test; RHAA bouts were defined as ≥3 efforts with ≤45 s recovery between efforts. Results were analysed via generalised linear mixed model and magnitude-based inferential statistics. On average, 8.0±4.6 and 5.1±3.5 bouts were detected in an entire game using T70% and T80%, respectively. When all positions were analysed together, there was a very-likely small difference in the number of RHAA bouts between first and second half for T70% and T80%, respectively. RHAA bouts occur frequently in elite youth football, with small differences between halves and between playing positions within the first or second half in most variables assessed.

Different Training Modalities Improve Energy Cost and Performance in Master Runners.

Purpose: The aim of this study was to compare the effects of continuous moderate-intensity and discontinuous high-intensity training on running performance in master runners. Methods: Thirty-four male master runners (47.2 ± 7.4 years) were assigned to three different groups: continuous moderate-intensity training (CMIT), discontinuous high-intensity training (DHIT), and control group (CON). CMIT and DHIT performed 8-week of supervised training (3 session·wk-1; ~35 km·wk-1) while CON maintained their normal training habits (3-4 session·wk-1; ~50 km·wk-1). Peak oxygen consumption ([Formula: see text]O2peak) and peak running speed (vpeak) during incremental treadmill exercise, gas exchange threshold (GET), speed at GET, energy cost of running (Cr), and 5-km performance were evaluated before and after training. Results: Following the training period, both CMIT and DHIT significantly reduced Cr (-4.4 and -4.9%, respectively, P < 0.05), increased speed at GET (3.4 and 5.7%, P < 0.05) and improved 5-km time-trial performance (3.1 and 2.2%, P < 0.05) whereas no differences were found for [Formula: see text]O2peak and GET (as %[Formula: see text]O2peak). After training, vpeak improved only for DHIT (6%, P < 0.05). No differences were found in any variable for CON. Conclusions: This study indicates that both CMIT and DHIT may positively affect running performance in middle-aged master runners. This improvement was achieved despite a significant reduction of the amount of weekly training volume.

Acute exercise alters skeletal muscle mitochondrial respiration and H2O2 emission in response to hyperinsulinemic-euglycemic clamp in middle-aged obese men.

Obesity, sedentary lifestyle and aging are associated with mitochondrial dysfunction and impaired insulin sensitivity. Acute exercise increases insulin sensitivity in skeletal muscle; however, whether mitochondria are involved in these processes remains unclear. The aim of this study was to investigate the effects of insulin stimulation at rest and after acute exercise on skeletal muscle mitochondrial respiratory function (JO2) and hydrogen peroxide emission (JH2O2), and the associations with insulin sensitivity in obese, sedentary men. Nine men (means ± SD: 57 ± 6 years; BMI 33 ± 5 kg.m2) underwent hyperinsulinemic-euglycemic clamps in two separate trials 1-3 weeks apart: one under resting conditions, and another 1 hour after high-intensity exercise (4x4 min cycling at 95% HRpeak). Muscle biopsies were obtained at baseline, and pre/post clamp to measure JO2 with high-resolution respirometry and JH2O2 via Amplex UltraRed from permeabilized fibers. Post-exercise, both JO2 and JH2O2 during ADP stimulated state-3/OXPHOS respiration were lower compared to baseline (P<0.05), but not after subsequent insulin stimulation. JH2O2 was lower post-exercise and after subsequent insulin stimulation compared to insulin stimulation in the rest trial during succinate supported state-4/leak respiration (P<0.05). In contrast, JH2O2 increased during complex-I supported leak respiration with insulin after exercise compared with resting conditions (P<0.05). Resting insulin sensitivity and JH2O2 during complex-I leak respiration were positively correlated (r = 0.77, P<0.05). We conclude that in obese, older and sedentary men, acute exercise modifies skeletal muscle mitochondrial respiration and H2O2 emission responses to hyperinsulinemia in a respiratory state-specific manner, which may have implications for metabolic diseases involving insulin resistance.

Long-term Practice with Domain-Specific Task Constraints Influences Perceptual Skills.

The long-term impact of practice with different task constraints on perceptual skill is relatively un-explored. This study examined the influence of extensive practice, i.e., more than a 1000 h of structured practice, with domain-specific task constraints on perceptual skill associated with the passing action. Despite performing the same passing skill, it is not known whether long-term exposure to specific soccer or futsal task constraints influences the players' attunement to environmental information. This study examined this issue by assessing the attention orientation of soccer (n = 24) and futsal players (n = 24) during modified games (6 vs. 6). Futsal players had higher scanning behavior during ball reception and control (40% more ball-player attention alternations) while soccer players mainly scanned the environment when not in ball possession (25% more attention alternations). We suggest that the behavioral differences found are elicited by the extensive domain-specific practice. That is, the higher number of players in soccer, and by a more intense game and easier to control ball in futsal. This study provides new insights into the long-term effects of practicing with specific task constraints.

Acute High-Intensity Interval Exercise-Induced Redox Signaling Is Associated with Enhanced Insulin Sensitivity in Obese Middle-Aged Men.

Background: Obesity and aging are associated with increased oxidative stress, activation of stress and mitogen activated protein kinases (SAPK), and the development of insulin resistance and metabolic disease. In contrast, acute exercise also increases oxidative stress and SAPK signaling, yet is reported to enhance insulin sensitivity and reduce the risk of metabolic disease. This study explored this paradox by investigating the effect of a single session of high-intensity interval-exercise (HIIE) on redox status, muscle SAPK and insulin protein signaling in eleven middle-aged obese men. Methods: Participants completed a 2 h hyperinsulinaemic-euglycaemic clamp at rest, and 60 min after HIIE (4 × 4 mins at 95% HRpeak; 2 min recovery periods), separated by 1-3 weeks. Results: Irrespective of exercise-induced changes to redox status, insulin stimulation both at rest and after HIIE similarly increased plasma superoxide dismutase activity, plasma catalase activity, and skeletal muscle 4-HNE; and significantly decreased plasma TBARS and hydrogen peroxide. The SAPK signaling pathways of p38 MAPK, NF-κB p65, and JNK, and the distal insulin signaling protein AS160Ser588, were activated with insulin stimulation at rest and to a greater extent with insulin stimulation after a prior bout of HIIE. Higher insulin sensitivity after HIIE was associated with higher insulin-stimulated SOD activity, JNK, p38 MAPK and NF-κB phosphorylation (r = 0.63, r = 0.71, r = 0.72, r = 0.71; p < 0.05, respectively). Conclusion:These findings support a role for redox homeostasis and SAPK signaling in insulin-stimulated glucose uptake which may contribute to the enhancement of insulin sensitivity in obese men 3 h after HIIE.

The effect of hyperinsulinaemic-euglycaemic clamp and exercise on bone remodeling markers in obese men.

Bone remodelling markers (BRMs) are suppressed following a glucose load and during glucose infusion. As exercise increases indices of bone health and improves glucose handling, we hypothesised that, at rest, hyperinsulinaemic-euglycaemic clamp will suppress BRMs in obese men and that exercise prior to the clamp will prevent this suppression. Eleven obese nondiabetic men (age 58.1±2.2 years, body mass index=33.1±1.4 kg m(-2) mean±s.e.m.) had a hyperinsulinaemic-euglycaemic clamp (HEC) at rest (Control) and 60 min post exercise (four bouts × 4 min cycling at 95% of hazard ratiopeak). Blood samples were analysed for serum insulin, glucose, bone formation markers, total osteocalcin (tOC) and procollagen type 1 N-terminal propeptide (P1NP), and the bone resorption marker, ß-isomerised C-terminal telopeptides (ß-CTx). In the control trial (no exercise), tOC, P1NP and ß-CTx decreased with HEC by >10% compared with baseline (P<0.05). Fasting serum glucose, but not insulin, tended to correlate negatively with the BRMs (ß range -0.57 to -0.66, p range 0.051-0.087). ß-CTx, but not OC or P1NP, increased within 60 min post exercise (∼16%, P<0.01). During the post-exercise HEC, the glucose infusion rate was ∼30% higher compared with the no exercise trial. Despite this, BRMs were only suppressed to a similar extent as in the control session (10%). HEC suppressed BRMs in obese men. Exercise did not prevent this suppression of BRMs by HEC but improved glucose handling during the trial. It remains to be tested whether an exercise intervention of longer duration may be able to prevent the effect of HEC on bone remodelling.

Training Diaries during Altitude Training Camp in Two Olympic Champions: An Observational Case Study.

Traditionally, Live High-Train High (LHTH) interventions were adopted when athletes trained and lived at altitude to try maximising the benefits offered by hypoxic exposure and improving sea level performance. Nevertheless, scientific research has proposed that the possible benefits of hypoxia would be offset by the inability to maintain high training intensity at altitude. However, elite athletes have been rarely recruited as an experimental sample, and training intensity has almost never been monitored during altitude research. This case study is an attempt to provide a practical example of successful LHTH interventions in two Olympic gold medal athletes. Training diaries were collected and total training volumes, volumes at different intensities, and sea level performance recorded before, during and after a 3-week LHTH camp. Both athletes successfully completed the LHTH camp (2090 m) maintaining similar absolute training intensity and training volume at high-intensity (> 91% of race pace) compared to sea level. After the LHTH intervention both athletes obtained enhancements in performance and they won an Olympic gold medal. In our opinion, LHTH interventions can be used as a simple, yet effective, method to maintain absolute, and improve relative training intensity in elite endurance athletes. Key PointsElite endurance athletes, with extensive altitude training experience, can maintain similar absolute intensity during LHTH compared to sea level.LHTH may be considered as an effective method to increase relative training intensity while maintaining the same running/walking pace, with possible beneficial effects on sea level performance.Training intensity could be the key factor for successful high-level LHTH camp.

The effect of acute exercise on undercarboxylated osteocalcin and insulin sensitivity in obese men.

Acute exercise improves insulin sensitivity for hours after the exercise is ceased. The skeleton contributes to glucose metabolism and insulin sensitivity via osteocalcin (OC) in its undercarboxylated (ucOC) form in mice. We tested the hypothesis that insulin sensitivity over the hours after exercise is associated with circulating levels of ucOC. Eleven middle-aged (58.1 ± 2.2 years mean ± SEM), obese (body mass index [BMI] = 33.1 ± 1.4 kg/m(2) ) nondiabetic men completed a euglycemic-hyperinsulinemic clamp at rest (rest-control) and at 60 minutes after exercise (4 × 4 minutes of cycling at 95% of HRpeak ). Insulin sensitivity was determined by glucose infusion rate relative to body mass (GIR, mL/kg/min) as well as GIR per unit of insulin (M-value). Blood samples and five muscle biopsies were obtained; two at the resting-control session, one before and one after clamping, and three in the exercise session, at rest, 60 minutes after exercise, and after the clamp. Exercise increased serum ucOC (6.4 ± 2.1%, p = 0.013) but not total OC (p > 0.05). Blood glucose was ∼6% lower and insulin sensitivity was ∼35% higher after exercise compared with control (both p < 0.05). Phosphorylated (P)-AKT (Ak thymoma) was higher after exercise and insulin compared with exercise alone (no insulin) and insulin alone (no exercise, all p < 0.05). In a multiple-linear regression including BMI, age, and aerobic fitness, ucOC was associated with whole-body insulin sensitivity at rest (ß = 0.59, p = 0.023) and after exercise (ß = 0.66, p = 0.005). Insulin sensitivity, after acute exercise, is associated with circulating levels of ucOC in obese men. Whether ucOC has a direct effect on skeletal muscle insulin sensitivity after exercise is yet to be determined.

The effects of osteoarthritis and age on skeletal muscle strength, Na+-K+-ATPase content, gene and isoform expression.

Knee osteoarthritis (OA) is a debilitating disorder prevalent in older populations that is accompanied by declines in muscle mass, strength, and physical activity. In skeletal muscle, the Na(+)-K(+) pump (NKA) is pivotal in ion homeostasis and excitability and is modulated by disuse and exercise training. This study examined the effects of OA and aging on muscle NKA in 36 older adults (range 55-81 yr), including 19 with OA (69.9 ± 6.5 yr, mean ± SD) and 17 asymptomatic controls (CON, 66.8 ± 6.4 yr). Participants completed knee extensor strength testing and a physical activity questionnaire. A vastus lateralis muscle biopsy was analyzed for NKA content ([(3)H]ouabain binding sites), α1-3- and ß1-3-isoform protein abundance (immunoblotting), and mRNA (real-time RT-PCR). The association between age and NKA content was investigated within the OA and CON groups and in pooled data. The NKA content was also contrasted between subgroups below and above the median age of 68.5 yr. OA had lower strength (-40.8%, P = 0.005), but higher NKA α2- (∼34%, P = 0.006) and α3-protein (100%, P = 0.016) abundance than CON and performed more incidental physical activity (P = 0.035). No differences were found between groups for NKA content, abundance of other NKA isoforms, or gene expression. There was a negative correlation between age and NKA content within OA (r = -0.63, P = 0.03) and with both groups combined (r = -0.47, P = 0.038). The NKA content was 25.5% lower in the older (69-81 yr) than in the younger (55-68 yr) subgroup. Hence older age, but not knee OA, was related to lowered muscle NKA content in older adults.